Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,509,102 G→T 100% S57R (AGC→AGA)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,509,1020GT99.1% 830.2 / ‑2.4 252S57R (AGC→AGA) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (126/123);  total (127/125)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 253 total aligned reads displayed.

ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248
                                                                                                                                                   |                                                                                                                                                  
acggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                  <  1:2768708/150‑1 (MQ=255)
 cggaccggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTGTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCAGCTTCACAATGTTATAGAGCCTGATCAGGTCTCTa                                                                                                                                                 >  1:2443282/1‑150 (MQ=255)
 cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                                 >  2:3194303/1‑150 (MQ=255)
  ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                                >  2:235351/1‑150 (MQ=255)
  ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                                >  2:708640/1‑150 (MQ=255)
   gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAtt                                                                                                                                               <  2:2780684/150‑1 (MQ=255)
    aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTg                                                                                                                                              <  1:1526334/150‑1 (MQ=255)
     acggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGa                                                                                                                                             <  2:4466683/150‑1 (MQ=255)
      cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGat                                                                                                                                            <  2:4811058/150‑1 (MQ=255)
       ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                           <  1:2916566/150‑1 (MQ=255)
       ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                           >  1:2588330/1‑150 (MQ=255)
       ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                           >  1:4481903/1‑150 (MQ=255)
          tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCg                                                                                                                                        <  1:3373539/150‑1 (MQ=255)
           ccTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCgg                                                                                                                                       >  1:96660/1‑150 (MQ=255)
            cTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGc                                                                                                                                      >  1:2512352/1‑150 (MQ=255)
             tGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCa                                                                                                                                     >  1:3017577/1‑150 (MQ=255)
               gCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAg                                                                                                                                   <  2:4714405/150‑1 (MQ=255)
                   ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGata                                                                                                                               >  1:2581458/1‑150 (MQ=255)
                    tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGatat                                                                                                                              <  1:2332407/150‑1 (MQ=255)
                     gttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGAtata                                                                                                                             <  1:1913554/150‑1 (MQ=255)
                       tgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAc                                                                                                                           <  2:1184670/150‑1 (MQ=255)
                       tgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAc                                                                                                                           >  1:635594/1‑150 (MQ=255)
                             ctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGaaa                                                                                                                     >  2:1539699/1‑150 (MQ=255)
                                   ggAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                               >  1:1026197/1‑150 (MQ=255)
                                   ggAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                               <  2:2473137/150‑1 (MQ=255)
                                    gAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgt                                                                                                              >  2:4309217/1‑150 (MQ=255)
                                     aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                                             <  2:4085021/150‑1 (MQ=255)
                                      aCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                            <  1:2881777/150‑1 (MQ=255)
                                       cTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                                                           <  2:3425799/150‑1 (MQ=255)
                                        tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                          <  2:1294291/150‑1 (MQ=255)
                                        tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                          <  1:160909/150‑1 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        >  2:3608000/1‑150 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        >  2:2007333/1‑150 (MQ=255)
                                          aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                        >  1:3322941/1‑150 (MQ=255)
                                             gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                     >  1:4154455/1‑150 (MQ=255)
                                                 aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAcc                                                                                                 <  1:3513784/150‑1 (MQ=255)
                                                 aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAcc                                                                                                 <  2:2929351/150‑1 (MQ=255)
                                                 aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAcc                                                                                                 <  1:4341864/150‑1 (MQ=255)
                                                   cTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGa                                                                                               >  2:3181254/1‑150 (MQ=255)
                                                   cTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGGTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGa                                                                                               >  2:2265478/1‑150 (MQ=255)
                                                    tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAc                                                                                              <  1:3076270/150‑1 (MQ=255)
                                                    tCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCCATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAc                                                                                              >  1:3811376/1‑150 (MQ=255)
                                                     cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAcc                                                                                             <  2:4141386/150‑1 (MQ=255)
                                                      ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                            >  1:2231965/1‑150 (MQ=255)
                                                          cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                        >  2:1708878/1‑150 (MQ=255)
                                                          cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                        <  2:1473156/150‑1 (MQ=255)
                                                           aaTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                       >  1:3012271/1‑150 (MQ=255)
                                                                gTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACa                                                                                  >  2:734099/1‑150 (MQ=255)
                                                                  ttCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTCCaaa                                                                                >  2:4102641/1‑150 (MQ=255)
                                                                   tCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCATTACGCTTATTCACATTGTTATAGAGACTGATCTGGTCTATATTGATATCGGAAAGTATTTAACGATAAATGTGGTTGTTGATAAACACCGACCCGGCACCTACaaaa                                                                               <  2:497765/150‑1 (MQ=255)
                                                                   tCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACaaaa                                                                               >  2:3891311/1‑150 (MQ=255)
                                                                   tCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACaaaa                                                                               >  1:4004987/1‑150 (MQ=255)
                                                                   tCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTAATCAGTACTCATATTCACAATGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTAGAAACACCGACCCGGCACCTACaaaa                                                                               <  2:3588866/150‑1 (MQ=255)
                                                                    cGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                              >  2:5017895/1‑150 (MQ=255)
                                                                     ggtaaaaaacaGCTCGGGTGCGGCATGTAGGTACTCATCAGTACGAATCTTCACATTGTTATAGAGACTGATCTGGTCTCTATTGATATCGGAATGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                                             <  1:4837213/144‑1 (MQ=255)
                                                                      ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                                                            >  1:5080987/1‑150 (MQ=255)
                                                                        aacaaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTc                                                                          <  2:5099319/150‑1 (MQ=255)
                                                                           aaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGACACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                       >  1:456687/1‑150 (MQ=255)
                                                                           aaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                       >  1:1734022/1‑150 (MQ=255)
                                                                            aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTTTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                      <  2:1799853/150‑1 (MQ=255)
                                                                              caGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACACAAGGCTCAACCAg                                                                    >  2:1900290/1‑150 (MQ=255)
                                                                                   cgcgTGCGGCCTGTACGTACTCATCAGTACGCATATTCAAAATGTTATAGAGACTTATCTGGTCTATATTTATATCGGCAAGGATATAACGAGAAATGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACAtt                                                               <  1:1499836/150‑1 (MQ=255)
                                                                                    gcgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTTTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTc                                                              <  1:2558716/150‑1 (MQ=255)
                                                                                      gTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGc                                                            >  1:1104149/1‑150 (MQ=255)
                                                                                        gCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGccc                                                          <  2:1580741/150‑1 (MQ=255)
                                                                                         cGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                                                         >  2:4046413/1‑150 (MQ=255)
                                                                                          ggCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCtt                                                        >  1:848126/1‑150 (MQ=255)
                                                                                          ggCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATCTAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACCTTCGCCCtt                                                        >  1:496975/1‑150 (MQ=255)
                                                                                             cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                     <  2:1319333/150‑1 (MQ=255)
                                                                                               gtacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACAGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACCAAAGGCTCAACCAGACATTCGCCCGTGGGCa                                                   >  2:634902/1‑150 (MQ=255)
                                                                                                tacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTATCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                                                  <  1:1971503/150‑1 (MQ=255)
                                                                                                 acgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaaa                                                 >  1:436910/1‑150 (MQ=255)
                                                                                                   gtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                                               <  2:3822083/150‑1 (MQ=255)
                                                                                                    tacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                                              <  2:836865/150‑1 (MQ=255)
                                                                                                     acTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGcc                                             >  1:1649398/1‑150 (MQ=255)
                                                                                                      cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                            <  2:3227163/150‑1 (MQ=255)
                                                                                                       tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                           >  1:1236774/1‑150 (MQ=255)
                                                                                                       tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                           >  2:3270137/1‑150 (MQ=255)
                                                                                                       taatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                           <  1:2846777/148‑1 (MQ=255)
                                                                                                          tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTa                                        >  1:3156933/1‑150 (MQ=255)
                                                                                                           caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa                                       <  2:2512352/150‑1 (MQ=255)
                                                                                                             gTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAata                                     >  1:1544407/1‑150 (MQ=255)
                                                                                                              tACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatat                                    >  2:4516853/1‑150 (MQ=255)
                                                                                                                 gCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcat                                 >  2:452139/1‑150 (MQ=255)
                                                                                                                     cTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAACTGCCGTTTAATATCATCAAg                             >  2:206989/1‑150 (MQ=255)
                                                                                                                     cTTCACAAGGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                             <  2:4129714/150‑1 (MQ=255)
                                                                                                                          caATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                        >  2:3569598/1‑150 (MQ=255)
                                                                                                                           aaTGTTATTGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAGTAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgggg                       <  1:4664096/150‑1 (MQ=255)
                                                                                                                           aaTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgggg                       <  1:4586050/150‑1 (MQ=255)
                                                                                                                             tGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAt                     <  2:1026197/150‑1 (MQ=255)
                                                                                                                             tGTTATAGAGACTGATCAGGACTATATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAt                     <  1:2345880/150‑1 (MQ=255)
                                                                                                                              gTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATa                    >  1:3485535/1‑150 (MQ=255)
                                                                                                                                      gaCTGAACAGGTCTCTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAACAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc            <  1:1792042/150‑1 (MQ=255)
                                                                                                                                       aCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGccccc           <  2:2581475/150‑1 (MQ=255)
                                                                                                                                           aTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc       <  1:4175849/150‑1 (MQ=255)
                                                                                                                                           aTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc       >  1:2087479/1‑150 (MQ=255)
                                                                                                                                           aTCAGGTATATATTTATATCGGCAAGGATATAACTAGAAAAGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGcc       <  1:3990358/150‑1 (MQ=255)
                                                                                                                                             ctggTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCa     <  1:4793018/148‑1 (MQ=255)
                                                                                                                                               ggTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACt   <  1:5036737/150‑1 (MQ=255)
                                                                                                                                                gTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGACCCCTGCCCACtt  <  2:2579287/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT  >  CP009273/3508955‑3509248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  >  CP009273/3508953‑3509251
                                                                                                                                                     |                                                                                                                                                     
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCT                                                                                                                                                       <  SRR19973063.3579059/150‑1 (MQ=60)
  ACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCT                                                                                                                                                     <  SRR19973063.2777633/150‑1 (MQ=60)
   CTGAACTGATCCTGTCTTTTGTTGAACTCTTCGCTGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTA                                                                                                                                                    <  SRR19973063.2674460/150‑1 (MQ=60)
   CGGACCGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTGTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCAGCTTCACAATGTTATAGAGCCTGATCAGGTCTCTA                                                                                                                                                    >  SRR19973063.2451086/1‑150 (MQ=60)
   CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTA                                                                                                                                                    >  SRR19973063.1805427/1‑150 (MQ=60)
   CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTA                                                                                                                                                    <  SRR19973063.4975708/150‑1 (MQ=60)
     GAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATT                                                                                                                                                  <  SRR19973063.3080553/150‑1 (MQ=60)
      AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                                 >  SRR19973063.1525201/1‑150 (MQ=60)
      AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                                 <  SRR19973063.1530988/150‑1 (MQ=60)
      AACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTG                                                                                                                                                 <  SRR19973063.1531009/150‑1 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                              >  SRR19973063.2596558/1‑150 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                              <  SRR19973063.1609159/150‑1 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                              >  SRR19973063.4496275/1‑150 (MQ=60)
         GGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATA                                                                                                                                              <  SRR19973063.2925868/150‑1 (MQ=60)
           ATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATC                                                                                                                                            <  SRR19973063.1647690/150‑1 (MQ=60)
            TCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCG                                                                                                                                           <  SRR19973063.3384283/150‑1 (MQ=60)
             CCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGG                                                                                                                                          >  SRR19973063.97059/1‑150 (MQ=60)
             CCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGG                                                                                                                                          >  SRR19973063.96989/1‑150 (MQ=60)
              CTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGC                                                                                                                                         >  SRR19973063.2520361/1‑150 (MQ=60)
               TGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCA                                                                                                                                        >  SRR19973063.3027206/1‑150 (MQ=60)
               TGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCA                                                                                                                                        >  SRR19973063.2587485/1‑150 (MQ=60)
               TGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCA                                                                                                                                        >  SRR19973063.2587491/1‑150 (MQ=60)
                GGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCATAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAA                                                                                                                                       <  SRR19973063.2937721/150‑1 (MQ=60)
                     TTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATA                                                                                                                                  >  SRR19973063.2589682/1‑150 (MQ=60)
                     TTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATA                                                                                                                                  >  SRR19973063.2589665/1‑150 (MQ=60)
                      TGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATAT                                                                                                                                 <  SRR19973063.1546341/150‑1 (MQ=60)
                      TGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATAT                                                                                                                                 <  SRR19973063.2339867/150‑1 (MQ=60)
                      TGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATAT                                                                                                                                 <  SRR19973063.4600076/150‑1 (MQ=60)
                       GTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATA                                                                                                                                <  SRR19973063.1919540/150‑1 (MQ=60)
                       GTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATA                                                                                                                                <  SRR19973063.4051750/150‑1 (MQ=60)
                         TGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAC                                                                                                                              >  SRR19973063.637464/1‑150 (MQ=60)
                         TGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAC                                                                                                                              >  SRR19973063.731642/1‑150 (MQ=60)
                          GAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACG                                                                                                                             >  SRR19973063.481918/1‑150 (MQ=60)
                            ACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAG                                                                                                                           >  SRR19973063.586458/1‑150 (MQ=60)
                             CTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGA                                                                                                                          <  SRR19973063.185598/150‑1 (MQ=60)
                              TCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAA                                                                                                                         >  SRR19973063.1733025/1‑150 (MQ=60)
                                     GGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGG                                                                                                                  >  SRR19973063.1029291/1‑150 (MQ=60)
                                     GGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGG                                                                                                                  >  SRR19973063.205795/1‑150 (MQ=60)
                                     GGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGG                                                                                                                  <  SRR19973063.1172034/150‑1 (MQ=60)
                                        ACTGATAGTCAACCTCGGCGCAATTTGGTTCGGGATCACACAGATCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCAAAATGTTATAGAGACTGTTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGT                                                                                                               <  SRR19973063.2891010/150‑1 (MQ=60)
                                        ACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGT                                                                                                               <  SRR19973063.2890992/150‑1 (MQ=60)
                                          TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                             <  SRR19973063.161406/150‑1 (MQ=60)
                                          TGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTG                                                                                                             >  SRR19973063.3929325/1‑150 (MQ=60)
                                           GATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGA                                                                                                            <  SRR19973063.2518848/150‑1 (MQ=60)
                                            ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAG                                                                                                           >  SRR19973063.3333551/1‑150 (MQ=60)
                                               GTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAA                                                                                                        >  SRR19973063.4167741/1‑150 (MQ=60)
                                               GTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAA                                                                                                        >  SRR19973063.4167766/1‑150 (MQ=60)
                                                TAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAAC                                                                                                       >  SRR19973063.4449078/1‑150 (MQ=60)
                                                   ACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACC                                                                                                    <  SRR19973063.4355715/150‑1 (MQ=60)
                                                   ACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACC                                                                                                    <  SRR19973063.3525022/150‑1 (MQ=60)
                                                    CCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCG                                                                                                   <  SRR19973063.2916267/150‑1 (MQ=60)
                                                      TCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAC                                                                                                 <  SRR19973063.3086148/150‑1 (MQ=60)
                                                      TCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAC                                                                                                 <  SRR19973063.3086154/150‑1 (MQ=60)
                                                      TCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCCATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAC                                                                                                 >  SRR19973063.3823506/1‑150 (MQ=60)
                                                        GGCGCAATTTGTTTCGGGAACAAACNGNTNGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTANAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCC                                                                                               >  SRR19973063.4671195/1‑150 (MQ=60)
                                                        GGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCC                                                                                               >  SRR19973063.2239040/1‑150 (MQ=60)
                                                         GCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCG                                                                                              <  SRR19973063.3557052/150‑1 (MQ=60)
                                                            AAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTAATCATTACGCATCTTCACAATTTTATAGAGACTGATCTGGTCTATTTTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCA                                                                                           <  SRR19973063.1975780/150‑1 (MQ=60)
                                                             AATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAC                                                                                          >  SRR19973063.3023908/1‑150 (MQ=60)
                                                             AATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAC                                                                                          >  SRR19973063.3021880/1‑150 (MQ=60)
                                                             AATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAC                                                                                          >  SRR19973063.2232495/1‑150 (MQ=60)
                                                                 TGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTAC                                                                                      >  SRR19973063.76678/1‑150 (MQ=60)
                                                                     TCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAA                                                                                  >  SRR19973063.4017832/1‑150 (MQ=60)
                                                                     TCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAA                                                                                  >  SRR19973063.4017755/1‑150 (MQ=60)
                                                                       GGTAAAAAACAGCTCGGGTGCGGCATGTAGGTACTCATCAGTACGAATCTTCACATTGTTATAGAGACTGATCTGGTCTCTATTGATATCGGAATGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGG                                                                                <  SRR19973063.4852752/150‑1 (MQ=60)
                                                                       GGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGG                                                                                >  SRR19973063.1183377/1‑150 (MQ=60)
                                                                        GGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGC                                                                               >  SRR19973063.5097328/1‑150 (MQ=60)
                                                                        GGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGC                                                                               >  SRR19973063.5097331/1‑150 (MQ=60)
                                                                           ACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCA                                                                            >  SRR19973063.2527098/1‑150 (MQ=60)
                                                                             AAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGACACACCGACCCGGCACCTACAAAAGGCTCAAC                                                                          >  SRR19973063.457975/1‑150 (MQ=60)
                                                                             AAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAC                                                                          >  SRR19973063.4325426/1‑150 (MQ=60)
                                                                             AAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAC                                                                          >  SRR19973063.1739303/1‑150 (MQ=60)
                                                                                CAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAG                                                                       >  SRR19973063.1089274/1‑150 (MQ=60)
                                                                                 AGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGA                                                                      >  SRR19973063.4596285/1‑150 (MQ=60)
                                                                                     CGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATT                                                                  >  SRR19973063.168973/1‑150 (MQ=60)
                                                                                     CGCGTGCGGCCTGTACGTACTCATCAGTACGCATATTCAAAATGTTATAGAGACTTATCTGGTCTATATTTATATCGGCAAGGATATAACGAGAAATGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATT                                                                  <  SRR19973063.1504428/150‑1 (MQ=60)
                                                                                      GCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTTTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTC                                                                 <  SRR19973063.2566865/150‑1 (MQ=60)
                                                                                        GTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGC                                                               >  SRR19973063.1107511/1‑150 (MQ=60)
                                                                                          GCGGCGTTTACTTACTCATCTTTACGAATCTTCACAATTTTATAGAGACTTATCCGGTCGCTTTTGATATCGGCAAGTATATAACGATAAATGTCGGTTTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCC                                                             <  SRR19973063.3968388/150‑1 (MQ=60)
                                                                                            GGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTT                                                           >  SRR19973063.850575/1‑150 (MQ=60)
                                                                                            GGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATCTAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACCTTCGCCCTT                                                           >  SRR19973063.498403/1‑150 (MQ=60)
                                                                                                TGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGC                                                       >  SRR19973063.1586297/1‑150 (MQ=60)
                                                                                                  TACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAA                                                     >  SRR19973063.2403207/1‑150 (MQ=60)
                                                                                                  TACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTATCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAA                                                     <  SRR19973063.1977670/150‑1 (MQ=60)
                                                                                                   ACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAA                                                    >  SRR19973063.438126/1‑150 (MQ=60)
                                                                                                    CGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGTTATCTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAT                                                   <  SRR19973063.3741654/150‑1 (MQ=60)
                                                                                                       ACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCC                                                >  SRR19973063.2277743/1‑150 (MQ=60)
                                                                                                       ACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCC                                                >  SRR19973063.1654432/1‑150 (MQ=60)
                                                                                                       ACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACACTCGCCCTTGGGCAAATGCC                                                >  SRR19973063.3373453/1‑150 (MQ=60)
                                                                                                         TCATCAGTACTAATCTTCACATTGTTATAGAGACTGATCTGGTCTCTATTGATATCGGCAAGGATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGT                                              <  SRR19973063.236061/150‑1 (MQ=60)
                                                                                                         TCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGT                                              >  SRR19973063.1240463/1‑150 (MQ=60)
                                                                                                         TAATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGT                                              <  SRR19973063.2855907/150‑1 (MQ=60)
                                                                                                          TATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTT                                             >  SRR19973063.3485763/1‑150 (MQ=60)
                                                                                                           ATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTT                                            >  SRR19973063.3014145/1‑150 (MQ=60)
                                                                                                            TCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTA                                           >  SRR19973063.3167094/1‑150 (MQ=60)
                                                                                                             CTGTACGCATCTACACAATGTTATAGAGACTGATCAGGTCTATTTTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAA                                          <  SRR19973063.2738976/150‑1 (MQ=60)
                                                                                                              AGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAT                                         >  SRR19973063.2290810/1‑150 (MQ=60)
                                                                                                               GTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATA                                        >  SRR19973063.1549141/1‑150 (MQ=60)
                                                                                                                 ACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATCTCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATC                                      <  SRR19973063.207489/150‑1 (MQ=60)
                                                                                                                 ACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATC                                      <  SRR19973063.250763/150‑1 (MQ=60)
                                                                                                                   GCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCAT                                    >  SRR19973063.3417603/1‑150 (MQ=60)
                                                                                                                    CATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATC                                   >  SRR19973063.955292/1‑150 (MQ=60)
                                                                                                                      TCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAA                                 >  SRR19973063.2842057/1‑150 (MQ=60)
                                                                                                                          CACAATGTTATAGAGACTTATCAGGTATCTTTTGATATCGGCAAGTATATAACTAGAAACGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAG                             <  SRR19973063.1791027/150‑1 (MQ=60)
                                                                                                                          CACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAG                             >  SRR19973063.4994618/1‑150 (MQ=60)
                                                                                                                          CACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAG                             >  SRR19973063.4696746/1‑150 (MQ=60)
                                                                                                                             AATGTTATTGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAAGTAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGG                          <  SRR19973063.4678972/150‑1 (MQ=60)
                                                                                                                             AATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGG                          <  SRR19973063.4600727/150‑1 (MQ=60)
                                                                                                                               TGTTATAGAGACTGATCAGGACTATATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAT                        <  SRR19973063.2353387/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTTATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                       <  SRR19973063.453965/150‑1 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                       >  SRR19973063.3496713/1‑150 (MQ=60)
                                                                                                                                GTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATA                       >  SRR19973063.3496669/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTTATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGTGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                  <  SRR19973063.2883809/150‑1 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                  <  SRR19973063.2883852/150‑1 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                  >  SRR19973063.3100425/1‑150 (MQ=60)
                                                                                                                                     AGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGC                  >  SRR19973063.1413636/1‑150 (MQ=60)
                                                                                                                                      GAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCC                 >  SRR19973063.509061/1‑150 (MQ=60)
                                                                                                                                        GACTGAACAGGTCTCTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAACAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCC               <  SRR19973063.1797587/150‑1 (MQ=60)
                                                                                                                                           TGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTG            >  SRR19973063.4124208/1‑150 (MQ=60)
                                                                                                                                           TGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGAGACTTGCCCCCTG            >  SRR19973063.4935917/1‑150 (MQ=60)
                                                                                                                                            GATAAGGTCTCTATTGATTTCGGCAAGGATATAACGATAAATGTCGGTGTTGAGAAACACCGACCCGGCACCTACAACAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGC           <  SRR19973063.432250/150‑1 (MQ=60)
                                                                                                                                             ATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC          >  SRR19973063.4436118/1‑150 (MQ=60)
                                                                                                                                             ATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC          <  SRR19973063.4189191/150‑1 (MQ=60)
                                                                                                                                             ATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC          >  SRR19973063.2094001/1‑150 (MQ=60)
                                                                                                                                             ATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCC          <  SRR19973063.960350/150‑1 (MQ=60)
                                                                                                                                               CTGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCA        <  SRR19973063.4808330/150‑1 (MQ=60)
                                                                                                                                               CAGGTCTCTATTGATATCGGCTAGTATATACCTAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCA        <  SRR19973063.4807931/150‑1 (MQ=60)
                                                                                                                                                 GGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACT      <  SRR19973063.5052972/150‑1 (MQ=60)
                                                                                                                                                 GGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACT      <  SRR19973063.3204573/150‑1 (MQ=60)
                                                                                                                                                 GGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACT      <  SRR19973063.3204533/150‑1 (MQ=60)
                                                                                                                                                  GTCTCTATTGATATCGGCAAGGATATAACGAGAAACGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTT     <  SRR19973063.4374031/150‑1 (MQ=60)
                                                                                                                                                     TCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  <  SRR19973063.142201/150‑1 (MQ=60)
                                                                                                                                                     TCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  <  SRR19973063.142497/150‑1 (MQ=60)
                                                                                                                                                     GATATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  <  SRR19973063.2913882/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAA  >  CP009273/3508953‑3509251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑