Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,798,834 G→A 18.2% pseudogene (1060/1476 nt) arpB → pseudogene, ankyrin repeats

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,798,8340GA18.2% 518.7 / 97.9 198pseudogene (1060/1476 nt)arpBpseudogene, ankyrin repeats
Reads supporting (aligned to +/- strand):  ref base G (81/81);  new base A (19/17);  total (100/98)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 203 total aligned reads displayed.

CTAACGAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGA  >  CP009273/1798685‑1798980
                                                                                                                                                     |                                                                                                                                                  
 tAACGAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATgg                                                                                                                                                   <  1:2063051/150‑1 (MQ=255)
    cGAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGa                                                                                                                                                >  2:585414/1‑150 (MQ=255)
     gAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAg                                                                                                                                               <  1:2880017/150‑1 (MQ=255)
     gAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAg                                                                                                                                               <  1:1660036/150‑1 (MQ=255)
      aaaCACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGc                                                                                                                                              >  2:2114573/1‑150 (MQ=255)
       aaCACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGcc                                                                                                                                             >  1:2360881/1‑150 (MQ=255)
       aaCACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGcc                                                                                                                                             >  1:2514039/1‑150 (MQ=255)
       aaCACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGcc                                                                                                                                             >  1:1766594/1‑150 (MQ=255)
            cTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGAt                                                                                                                                        >  2:1332175/1‑150 (MQ=255)
             tGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGTCAGGAtt                                                                                                                                       >  1:2247934/1‑150 (MQ=255)
                 cTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATAc                                                                                                                                   >  1:3579696/1‑150 (MQ=255)
                      tatCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATAcgccgc                                                                                                                              >  1:2849353/1‑150 (MQ=255)
                           ccATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATgg                                                                                                                         <  2:3116523/150‑1 (MQ=255)
                            cATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGa                                                                                                                        >  2:1125093/1‑150 (MQ=255)
                               gttgtATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGaaaa                                                                                                                     <  1:1926095/150‑1 (MQ=255)
                               gttgtATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGaaaa                                                                                                                     >  1:3286571/1‑150 (MQ=255)
                                 tgtATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAaata                                                                                                                   >  2:174088/1‑150 (MQ=255)
                                  gtATTGATTATATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGATCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAaataa                                                                                                                  <  1:2219311/150‑1 (MQ=255)
                                    aTGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATc                                                                                                                <  2:2351547/150‑1 (MQ=255)
                                    aTGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATc                                                                                                                <  1:897979/150‑1 (MQ=255)
                                     tGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCa                                                                                                               >  2:3471521/1‑150 (MQ=255)
                                     tGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCa                                                                                                               <  1:774028/150‑1 (MQ=255)
                                         ttCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCt                                                                                                           <  1:940598/150‑1 (MQ=255)
                                           cGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCttt                                                                                                         >  1:1147886/1‑150 (MQ=255)
                                            gATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTtg                                                                                                        >  1:674608/1‑150 (MQ=255)
                                             aTGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTtgt                                                                                                       >  1:3981354/1‑150 (MQ=255)
                                               gAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTtgtgt                                                                                                     >  1:472597/1‑150 (MQ=255)
                                                     atCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTcaca                                                                                               >  2:2457047/1‑150 (MQ=255)
                                                       cGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACg                                                                                             <  2:1912541/150‑1 (MQ=255)
                                                       cGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACg                                                                                             <  2:3653374/150‑1 (MQ=255)
                                                          tatCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGtt                                                                                          >  1:1275260/1‑150 (MQ=255)
                                                              tttCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCctc                                                                                      >  2:4323444/1‑150 (MQ=255)
                                                              tttCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCctc                                                                                      >  1:3109999/1‑150 (MQ=255)
                                                                     aTGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTaaaa                                                                               >  1:2553193/1‑150 (MQ=255)
                                                                      tGCCTTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAAt                                                                              <  2:1061310/150‑1 (MQ=255)
                                                                      tGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAAt                                                                              >  2:3837897/1‑150 (MQ=255)
                                                                      tGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAAt                                                                              <  1:1207461/150‑1 (MQ=255)
                                                                         aTTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTaa                                                                           >  2:2274073/1‑150 (MQ=255)
                                                                          ttAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAAt                                                                          <  1:585414/150‑1 (MQ=255)
                                                                            aaCCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCTAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATgg                                                                        <  2:1825740/150‑1 (MQ=255)
                                                                            aaCCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATgg                                                                        <  1:2970886/150‑1 (MQ=255)
                                                                               cACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                     <  1:3015530/150‑1 (MQ=255)
                                                                               cACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                     >  2:1557122/1‑150 (MQ=255)
                                                                               cACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                     <  1:1911112/150‑1 (MQ=255)
                                                                                 cTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCg                                                                   <  1:3897299/150‑1 (MQ=255)
                                                                                  tCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGc                                                                  >  1:51299/1‑150 (MQ=255)
                                                                                  tCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGc                                                                  >  1:2226599/1‑150 (MQ=255)
                                                                                   ccAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGcc                                                                 >  2:1706915/1‑150 (MQ=255)
                                                                                     aaTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCtt                                                               <  1:3548903/150‑1 (MQ=255)
                                                                                     aaTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCtt                                                               >  2:3347795/1‑150 (MQ=255)
                                                                                      aTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCttt                                                              <  2:2947272/150‑1 (MQ=255)
                                                                                        aGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTaa                                                            >  1:2804603/1‑150 (MQ=255)
                                                                                        aGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTaa                                                            >  1:3700528/1‑150 (MQ=255)
                                                                                            caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATAc                                                        >  2:1025968/1‑150 (MQ=255)
                                                                                              agagCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACaa                                                      >  2:49012/1‑150 (MQ=255)
                                                                                                agCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGt                                                    >  1:3443249/1‑150 (MQ=255)
                                                                                                 gCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAACATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGtt                                                   <  2:3845173/150‑1 (MQ=255)
                                                                                                  cTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTg                                                  >  2:660947/1‑150 (MQ=255)
                                                                                                   ttttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGa                                                 >  1:3604296/1‑150 (MQ=255)
                                                                                                   ttttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGa                                                 <  2:2906375/150‑1 (MQ=255)
                                                                                                    tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAg                                                >  2:1518074/1‑150 (MQ=255)
                                                                                                    tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAg                                                >  2:1927611/1‑150 (MQ=255)
                                                                                                     ttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGc                                               <  2:355652/150‑1 (MQ=255)
                                                                                                      tAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCa                                              <  2:3569342/150‑1 (MQ=255)
                                                                                                       aaaCAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCaa                                             <  2:840070/150‑1 (MQ=255)
                                                                                                        aaCAAAAAACTTGTTATGAGTTTTTTAGCCATGAAAATAAATGATGGTGAGCCAGGATTATACGCCGCATTGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCaac                                            <  1:4338483/150‑2 (MQ=255)
                                                                                                         aCAAAAAACTGGTGATGAGTATATTAGACATGAAAATACATGATAGTGAGCCAGGATTATACGCCGAAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAg                                           <  2:3684970/150‑1 (MQ=255)
                                                                                                           aaaaaaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCt                                         >  2:4266639/1‑150 (MQ=255)
                                                                                                              aaaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAAc                                      >  2:2934006/1‑150 (MQ=255)
                                                                                                                 cTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAAcatc                                   <  1:42658/150‑1 (MQ=255)
                                                                                                                  tGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAAcatca                                  >  1:2159350/1‑150 (MQ=255)
                                                                                                                     tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                               >  1:4161338/1‑150 (MQ=255)
                                                                                                                     tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                               <  1:2165543/150‑1 (MQ=255)
                                                                                                                     tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                               >  1:4162000/1‑150 (MQ=255)
                                                                                                                        tgaGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAtt                            >  1:3993633/1‑150 (MQ=255)
                                                                                                                         gaGTATTTTAGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAttt                           <  1:3502593/150‑1 (MQ=255)
                                                                                                                          ggTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATtta                          >  2:3078832/2‑150 (MQ=255)
                                                                                                                           gTATTTTAGCCATGAAAATACATGATGGTGAGTCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttat                         <  2:2247934/150‑1 (MQ=255)
                                                                                                                            tATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatt                        <  1:3848146/150‑1 (MQ=255)
                                                                                                                            tATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatt                        >  2:4151910/1‑150 (MQ=255)
                                                                                                                            tATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatt                        >  2:1003221/1‑150 (MQ=255)
                                                                                                                             aTTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatta                       >  1:3952432/1‑150 (MQ=255)
                                                                                                                              ttttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaa                      >  1:475773/1‑150 (MQ=255)
                                                                                                                              ttttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaa                      >  1:1783038/1‑150 (MQ=255)
                                                                                                                                ttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaaaa                    <  1:333619/150‑1 (MQ=255)
                                                                                                                                  aGCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAgg                  <  1:1500575/150‑1 (MQ=255)
                                                                                                                                   gCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGa                 <  1:1866770/150‑1 (MQ=255)
                                                                                                                                   gCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGa                 <  1:4098038/150‑1 (MQ=255)
                                                                                                                                   gCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGa                 <  1:4056250/150‑1 (MQ=255)
                                                                                                                                   gCCATGAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGa                 >  1:2484771/1‑150 (MQ=255)
                                                                                                                                    ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAg                <  2:3287617/150‑1 (MQ=255)
                                                                                                                                    ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAg                <  2:3175220/150‑1 (MQ=255)
                                                                                                                                        gAAAATACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTAc            >  2:2176786/1‑150 (MQ=255)
                                                                                                                                         aaaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa           <  2:2699765/150‑1 (MQ=255)
                                                                                                                                         aaaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa           <  2:3916667/150‑1 (MQ=255)
                                                                                                                                         aaaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa           <  2:4003887/150‑1 (MQ=255)
                                                                                                                                         aaaaTACATGATAGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa           <  2:745676/150‑1 (MQ=255)
                                                                                                                                              aCATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGcaca      <  1:3154786/150‑1 (MQ=255)
                                                                                                                                                atgataGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAgg    >  2:3217060/1‑150 (MQ=255)
                                                                                                                                                  gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGa  >  2:2297670/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTAACGAAACACCTGGACTTTATATCTCCATGTTGTATGGATTCGATGAAATAATCGATATCTTTCTGAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGA  >  CP009273/1798685‑1798980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A