Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,017,285 G→T 89.2% D871Y (GAC→TAC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,017,2850GT89.2% 728.7 / 74.2 257D871Y (GAC→TAC) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base G (19/9);  new base T (119/110);  total (138/119)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGT  >  CP009273/3017138‑3017431
                                                                                                                                                   |                                                                                                                                                  
aaCACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAc                                                                                                                                                  >  1:3332089/1‑150 (MQ=255)
 acacGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACg                                                                                                                                                 <  2:274166/150‑1 (MQ=255)
  cacGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGc                                                                                                                                                >  1:940109/1‑150 (MQ=255)
          cGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTc                                                                                                                                        <  2:3596038/150‑1 (MQ=255)
             gTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCAcc                                                                                                                                     <  2:600266/150‑1 (MQ=255)
               gAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCg                                                                                                                                   <  1:3729353/150‑1 (MQ=255)
                aaaTCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGt                                                                                                                                  >  1:1184583/1‑150 (MQ=255)
                   tCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTaa                                                                                                                               >  1:275786/1‑150 (MQ=255)
                     cGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcg                                                                                                                             <  1:76308/150‑1 (MQ=255)
                     cGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcg                                                                                                                             <  1:2410240/150‑1 (MQ=255)
                      gCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgc                                                                                                                            >  2:3906726/1‑150 (MQ=255)
                      gCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgc                                                                                                                            >  2:1997817/1‑150 (MQ=255)
                       ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgcg                                                                                                                           <  1:491560/150‑1 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                         >  2:1315288/1‑150 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                         >  1:337613/1‑150 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                         >  1:2511620/1‑150 (MQ=255)
                          ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                        >  2:1052556/1‑150 (MQ=255)
                          ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                        >  1:736626/1‑150 (MQ=255)
                          ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                        >  2:93662/1‑150 (MQ=255)
                            aTAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGAtt                                                                                                                      <  1:274255/150‑1 (MQ=255)
                              aaaTTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTa                                                                                                                    >  1:1007770/1‑150 (MQ=255)
                                      cGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTa                                                                                                            <  2:1917535/150‑1 (MQ=255)
                                       gCCTTGCTGGACTGCTGTACACCGGTCAATCCAGAGTTAGCGTTGGGACACATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTAc                                                                                                           <  2:152452/150‑1 (MQ=255)
                                       gCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTAc                                                                                                           <  1:3406186/150‑1 (MQ=255)
                                         cTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACgg                                                                                                         >  2:3160496/1‑150 (MQ=255)
                                         cTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACgg                                                                                                         <  2:2028360/150‑1 (MQ=255)
                                          ttGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGgc                                                                                                        >  1:1850868/1‑150 (MQ=255)
                                          ttGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCGACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGgc                                                                                                        <  2:2042438/150‑1 (MQ=255)
                                           tGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGgcg                                                                                                       <  2:2242128/150‑1 (MQ=255)
                                             cTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCa                                                                                                     >  1:3277674/1‑150 (MQ=255)
                                               ggACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAcc                                                                                                   >  1:3614157/1‑150 (MQ=255)
                                               ggACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAcc                                                                                                   >  1:3745465/1‑150 (MQ=255)
                                                 aCTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAccta                                                                                                 >  2:3498040/1‑148 (MQ=255)
                                                   tGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGaaa                                                                                               <  2:1288080/150‑1 (MQ=255)
                                                    gCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGaaaa                                                                                              <  2:2122228/150‑1 (MQ=255)
                                                     cGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAAt                                                                                             <  2:4281012/150‑1 (MQ=255)
                                                      ggTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAAtt                                                                                            <  1:2922734/150‑1 (MQ=255)
                                                       gTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTg                                                                                           >  1:1138980/1‑150 (MQ=255)
                                                         acacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGt                                                                                         <  2:3069418/150‑1 (MQ=255)
                                                         acacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGt                                                                                         <  2:739172/150‑1 (MQ=255)
                                                         acacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGt                                                                                         <  2:2500238/150‑1 (MQ=255)
                                                          cacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTg                                                                                        >  1:1736662/1‑150 (MQ=255)
                                                           acCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGa                                                                                       >  1:4165287/1‑150 (MQ=255)
                                                            ccGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGAc                                                                                      <  2:3743632/150‑1 (MQ=255)
                                                            ccGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCAGCGTGCTATCGCCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGAc                                                                                      <  1:1049638/150‑1 (MQ=255)
                                                              ggTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAt                                                                                    >  1:3178880/1‑150 (MQ=255)
                                                                   aTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgc                                                                               <  1:823181/150‑1 (MQ=255)
                                                                    tCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgcg                                                                              <  2:608122/150‑1 (MQ=255)
                                                                      cAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTg                                                                            <  1:2035358/150‑1 (MQ=255)
                                                                       agagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTCACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGa                                                                           >  1:161255/1‑150 (MQ=255)
                                                                       agagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGa                                                                           >  1:2333457/1‑150 (MQ=255)
                                                                       agagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGa                                                                           >  2:2161419/1‑150 (MQ=255)
                                                                         agTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAtt                                                                         <  1:2287849/150‑1 (MQ=255)
                                                                            tAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTcc                                                                      <  1:1955340/150‑1 (MQ=255)
                                                                             aGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcg                                                                     >  1:354472/1‑150 (MQ=255)
                                                                             aGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATGTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcg                                                                     >  2:3851813/1‑150 (MQ=255)
                                                                               cGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcgcg                                                                   >  1:2653744/1‑150 (MQ=255)
                                                                                gTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc                                                                  >  2:176556/1‑150 (MQ=255)
                                                                                gTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc                                                                  >  2:2435644/1‑150 (MQ=255)
                                                                                  tGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCtg                                                                >  2:3658908/1‑150 (MQ=255)
                                                                                       cAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTg                                                           >  2:1744753/1‑150 (MQ=255)
                                                                                        aaaTCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTgg                                                          >  1:1353585/1‑150 (MQ=255)
                                                                                         aaTCTACGGTGCCACCCTGCGTACTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACTCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGt                                                         <  2:3615269/150‑1 (MQ=255)
                                                                                                ggTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                                  >  2:1978442/1‑150 (MQ=255)
                                                                                                  tGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcga                                                <  2:3961490/150‑1 (MQ=255)
                                                                                                   gCCACCCTGCGAGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgac                                               <  2:1060092/150‑1 (MQ=255)
                                                                                                         cTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaaa                                         >  1:1562765/1‑150 (MQ=255)
                                                                                                         cTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaaa                                         >  2:843473/1‑150 (MQ=255)
                                                                                                            cGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTc                                      <  2:2914945/150‑1 (MQ=255)
                                                                                                             gTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCg                                     >  1:1287807/1‑150 (MQ=255)
                                                                                                              tGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCgg                                    <  2:3901411/150‑1 (MQ=255)
                                                                                                                cTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                  <  2:1098223/150‑1 (MQ=255)
                                                                                                                cTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                  >  1:3999978/1‑150 (MQ=255)
                                                                                                                 tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                                 <  1:919691/150‑1 (MQ=255)
                                                                                                                 tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                                 <  2:3022110/150‑1 (MQ=255)
                                                                                                                 tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                                 >  2:4268513/1‑150 (MQ=255)
                                                                                                                  aTCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCg                                <  2:3379338/150‑1 (MQ=255)
                                                                                                                  aTCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCg                                <  2:1184583/150‑1 (MQ=255)
                                                                                                                        cacaGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                          >  2:890111/1‑150 (MQ=255)
                                                                                                                          caGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGc                        >  1:4108309/1‑150 (MQ=255)
                                                                                                                            gTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGa                      >  2:3499344/1‑150 (MQ=255)
                                                                                                                             tATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGaa                     <  2:4102510/150‑1 (MQ=255)
                                                                                                                              aTGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGaaa                    >  2:860430/1‑150 (MQ=255)
                                                                                                                               tGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                   <  2:3313237/150‑1 (MQ=255)
                                                                                                                               tGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                   <  2:3592115/150‑1 (MQ=255)
                                                                                                                                gAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATc                  <  2:1697265/150‑1 (MQ=255)
                                                                                                                                gAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATc                  <  2:3886653/150‑1 (MQ=255)
                                                                                                                                 aGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCg                 >  2:1428216/1‑150 (MQ=255)
                                                                                                                                 aGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCg                 >  1:3094476/1‑150 (MQ=255)
                                                                                                                                 aGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCg                 <  2:3430883/150‑1 (MQ=255)
                                                                                                                                  gCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGa                >  1:2172588/1‑150 (MQ=255)
                                                                                                                                   cGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt               <  2:3829390/150‑1 (MQ=255)
                                                                                                                                    gAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc              <  1:2593742/150‑1 (MQ=255)
                                                                                                                                      agagATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtctc            >  2:15138/1‑150 (MQ=255)
                                                                                                                                         gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGa         >  1:2631332/1‑150 (MQ=255)
                                                                                                                                         gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGa         >  2:309113/1‑150 (MQ=255)
                                                                                                                                          aTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaa        >  1:3489406/1‑150 (MQ=255)
                                                                                                                                          aTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaa        <  2:500911/150‑1 (MQ=255)
                                                                                                                                            cATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAt      <  1:4026763/150‑1 (MQ=255)
                                                                                                                                            cATTTAGTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAt      <  2:1673048/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGT  >  CP009273/3017138‑3017431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  CP009273/3017136‑3017433
                                                                                                                                                     |                                                                                                                                                    
TCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATT                                                                                                                                                      <  SRR19973079.1177087/150‑1 (MQ=60)
TCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATT                                                                                                                                                      <  SRR19973079.1308571/150‑1 (MQ=60)
  AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973079.3349166/1‑150 (MQ=60)
  AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973079.3349160/1‑150 (MQ=60)
  AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973079.3123429/1‑150 (MQ=60)
  AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973079.2505142/1‑150 (MQ=60)
    CACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGC                                                                                                                                                  >  SRR19973079.945526/1‑150 (MQ=60)
    CACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGC                                                                                                                                                  >  SRR19973079.945492/1‑150 (MQ=60)
    CACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGC                                                                                                                                                  >  SRR19973079.418835/1‑150 (MQ=60)
     ACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCC                                                                                                                                                 <  SRR19973079.407130/150‑1 (MQ=60)
        CGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAA                                                                                                                                              >  SRR19973079.1019028/1‑150 (MQ=60)
                 GAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCG                                                                                                                                     <  SRR19973079.3748456/150‑1 (MQ=60)
                  AAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGT                                                                                                                                    >  SRR19973079.3396752/1‑150 (MQ=60)
                  AAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATATATGACGCCGAAGGTCACCCGT                                                                                                                                    >  SRR19973079.1191400/1‑150 (MQ=60)
                    ATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTA                                                                                                                                  >  SRR19973079.2825360/1‑150 (MQ=60)
                     TCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAA                                                                                                                                 >  SRR19973079.277506/1‑150 (MQ=60)
                       CGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACG                                                                                                                               <  SRR19973079.2422714/150‑1 (MQ=60)
                       CGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACG                                                                                                                               <  SRR19973079.2422672/150‑1 (MQ=60)
                       CGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACG                                                                                                                               <  SRR19973079.77026/150‑1 (MQ=60)
                       CGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACG                                                                                                                               >  SRR19973079.2165405/1‑150 (MQ=60)
                         CCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCG                                                                                                                             <  SRR19973079.494547/150‑1 (MQ=60)
                           TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           >  SRR19973079.2524341/1‑150 (MQ=60)
                           TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           <  SRR19973079.2412569/150‑1 (MQ=60)
                           TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           >  SRR19973079.339490/1‑150 (MQ=60)
                            GGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGA                                                                                                                          >  SRR19973079.2420313/1‑150 (MQ=60)
                            GGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGA                                                                                                                          >  SRR19973079.2738028/1‑150 (MQ=60)
                            GGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGA                                                                                                                          >  SRR19973079.348363/1‑150 (MQ=60)
                            GGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGA                                                                                                                          >  SRR19973079.740656/1‑150 (MQ=60)
                            GGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGA                                                                                                                          >  SRR19973079.740536/1‑150 (MQ=60)
                             GATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGAT                                                                                                                         >  SRR19973079.85505/1‑150 (MQ=60)
                              ATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATT                                                                                                                        <  SRR19973079.275973/150‑1 (MQ=60)
                              ATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATT                                                                                                                        <  SRR19973079.275980/150‑1 (MQ=60)
                              ATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATT                                                                                                                        >  SRR19973079.1268389/1‑150 (MQ=60)
                                AAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTA                                                                                                                      >  SRR19973079.1013443/1‑150 (MQ=60)
                                  ATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACG                                                                                                                    >  SRR19973079.3762814/1‑150 (MQ=60)
                                         GCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTAC                                                                                                             <  SRR19973079.3423766/150‑1 (MQ=60)
                                            TTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGC                                                                                                          >  SRR19973079.1860646/1‑150 (MQ=60)
                                               CTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCA                                                                                                       >  SRR19973079.3294273/1‑150 (MQ=60)
                                               CTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCA                                                                                                       >  SRR19973079.3294962/1‑150 (MQ=60)
                                                 GGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACC                                                                                                     >  SRR19973079.3764661/1‑150 (MQ=60)
                                                 GGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACC                                                                                                     >  SRR19973079.3632498/1‑150 (MQ=60)
                                                 GGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACC                                                                                                     >  SRR19973079.3632465/1‑150 (MQ=60)
                                                      GCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAA                                                                                                <  SRR19973079.2615455/150‑1 (MQ=60)
                                                      GCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAA                                                                                                <  SRR19973079.431410/150‑1 (MQ=60)
                                                        GGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATT                                                                                              <  SRR19973079.2937614/150‑1 (MQ=60)
                                                         GTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTG                                                                                             >  SRR19973079.1145505/1‑150 (MQ=60)
                                                            CACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTG                                                                                          >  SRR19973079.3498416/1‑150 (MQ=60)
                                                            CACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTG                                                                                          >  SRR19973079.1745874/1‑150 (MQ=60)
                                                            CACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTG                                                                                          >  SRR19973079.1746063/1‑150 (MQ=60)
                                                             ACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGA                                                                                         >  SRR19973079.641295/1‑150 (MQ=60)
                                                             ACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGA                                                                                         >  SRR19973079.561007/1‑150 (MQ=60)
                                                             ACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGA                                                                                         >  SRR19973079.4186419/1‑150 (MQ=60)
                                                             ACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGA                                                                                         <  SRR19973079.2798419/150‑1 (MQ=60)
                                                              CCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGAC                                                                                        <  SRR19973079.1055446/150‑1 (MQ=60)
                                                              CCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGAC                                                                                        <  SRR19973079.2293579/150‑1 (MQ=60)
                                                              CCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCAGCGTGCTATCGCCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGAC                                                                                        <  SRR19973079.1055426/150‑1 (MQ=60)
                                                                GGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAT                                                                                      >  SRR19973079.3195144/1‑150 (MQ=60)
                                                                  TCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTC                                                                                    <  SRR19973079.3282087/150‑1 (MQ=60)
                                                                    AATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCG                                                                                  <  SRR19973079.3081302/150‑1 (MQ=60)
                                                                    AATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCG                                                                                  <  SRR19973079.3082255/150‑1 (MQ=60)
                                                                     ATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGC                                                                                 <  SRR19973079.827921/150‑1 (MQ=60)
                                                                        CAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTG                                                                              <  SRR19973079.2046059/150‑1 (MQ=60)
                                                                        CAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTG                                                                              <  SRR19973079.94525/150‑1 (MQ=60)
                                                                         AGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTCACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGA                                                                             >  SRR19973079.162496/1‑150 (MQ=60)
                                                                         AGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGA                                                                             >  SRR19973079.2345582/1‑150 (MQ=60)
                                                                           AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           <  SRR19973079.2299774/150‑1 (MQ=60)
                                                                           AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           <  SRR19973079.1986597/150‑1 (MQ=60)
                                                                           AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           <  SRR19973079.1987385/150‑1 (MQ=60)
                                                                           AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           <  SRR19973079.2299722/150‑1 (MQ=60)
                                                                              TAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCC                                                                        <  SRR19973079.1965753/150‑1 (MQ=60)
                                                                              TAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCC                                                                        <  SRR19973079.1527933/150‑1 (MQ=60)
                                                                               AGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCG                                                                       >  SRR19973079.356392/1‑150 (MQ=60)
                                                                                GCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGC                                                                      <  SRR19973079.1663416/150‑1 (MQ=60)
                                                                                GCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGC                                                                      <  SRR19973079.1663285/150‑1 (MQ=60)
                                                                                 CGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCG                                                                     >  SRR19973079.2667578/1‑150 (MQ=60)
                                                                                 CGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCG                                                                     >  SRR19973079.2667477/1‑150 (MQ=60)
                                                                                   TTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCT                                                                   <  SRR19973079.1303596/150‑1 (MQ=60)
                                                                                   TTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCT                                                                   <  SRR19973079.3371326/150‑1 (MQ=60)
                                                                                    TGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTG                                                                  <  SRR19973079.1475882/150‑1 (MQ=60)
                                                                                     GGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGT                                                                 <  SRR19973079.1129420/150‑1 (MQ=60)
                                                                                          AAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGG                                                            >  SRR19973079.1361144/1‑150 (MQ=60)
                                                                                                         CCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATA                                             <  SRR19973079.1257091/150‑1 (MQ=60)
                                                                                                           CTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAA                                           >  SRR19973079.1571386/1‑150 (MQ=60)
                                                                                                           CTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAA                                           <  SRR19973079.2593061/150‑1 (MQ=60)
                                                                                                           CTGCGTGCTATCGGCCACAGTAGGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAA                                           <  SRR19973079.406706/150‑1 (MQ=60)
                                                                                                            TGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAG                                          <  SRR19973079.3209296/150‑1 (MQ=60)
                                                                                                             GCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGT                                         >  SRR19973079.3544461/1‑150 (MQ=60)
                                                                                                             GCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGT                                         >  SRR19973079.3544485/1‑150 (MQ=60)
                                                                                                               GTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCG                                       >  SRR19973079.2653662/1‑150 (MQ=60)
                                                                                                               GTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCG                                       >  SRR19973079.1295133/1‑150 (MQ=60)
                                                                                                                  CTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCC                                    >  SRR19973079.4020052/1‑150 (MQ=60)
                                                                                                                  CTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCC                                    >  SRR19973079.4020054/1‑150 (MQ=60)
                                                                                                                   TATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCC                                   <  SRR19973079.1858199/150‑1 (MQ=60)
                                                                                                                   TATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCC                                   <  SRR19973079.925012/150‑1 (MQ=60)
                                                                                                                        GCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCG                              >  SRR19973079.1912964/1‑150 (MQ=60)
                                                                                                                          CACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGG                            <  SRR19973079.2612647/150‑1 (MQ=60)
                                                                                                                           ACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGG                           >  SRR19973079.821122/1‑150 (MQ=60)
                                                                                                                           ACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGG                           >  SRR19973079.821190/1‑150 (MQ=60)
                                                                                                                            CAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGC                          >  SRR19973079.4129148/1‑150 (MQ=60)
                                                                                                                            CAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGC                          >  SRR19973079.4130822/1‑150 (MQ=60)
                                                                                                                               TATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAA                       >  SRR19973079.3697869/1‑150 (MQ=60)
                                                                                                                                 TGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAT                     >  SRR19973079.1578819/1‑150 (MQ=60)
                                                                                                                                  GAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATC                    >  SRR19973079.1175133/1‑150 (MQ=60)
                                                                                                                                   AGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCG                   >  SRR19973079.3110469/1‑150 (MQ=60)
                                                                                                                                    GCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGA                  >  SRR19973079.2183679/1‑150 (MQ=60)
                                                                                                                                     CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 >  SRR19973079.3595153/1‑150 (MQ=60)
                                                                                                                                     CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 >  SRR19973079.2190158/1‑150 (MQ=60)
                                                                                                                                     CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 >  SRR19973079.96081/1‑150 (MQ=60)
                                                                                                                                      GAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATC                <  SRR19973079.2607053/150‑1 (MQ=60)
                                                                                                                                           GATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGA           >  SRR19973079.2644923/1‑150 (MQ=60)
                                                                                                                                            ATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAA          >  SRR19973079.3507361/1‑150 (MQ=60)
                                                                                                                                              CATTTATTACGCCGAAGGTCACCCGTTACCGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAGTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAT        <  SRR19973079.4047084/150‑1 (MQ=60)
                                                                                                                                                TTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCG      <  SRR19973079.379277/150‑1 (MQ=60)
                                                                                                                                                TTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCG      <  SRR19973079.377250/150‑1 (MQ=60)
                                                                                                                                                   ATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTG   >  SRR19973079.623051/1‑150 (MQ=60)
                                                                                                                                                    TTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  SRR19973079.1694732/1‑150 (MQ=60)
                                                                                                                                                    TTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  SRR19973079.1695441/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  CP009273/3017136‑3017433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑