Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,017,285 G→T 100% D871Y (GAC→TAC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,017,2850GT99.5% 785.0 / ‑4.1 218D871Y (GAC→TAC) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (114/103);  total (115/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGG  >  CP009273/3017138‑3017430
                                                                                                                                                   |                                                                                                                                                 
   acGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGcc                                                                                                                                              <  1:4151296/150‑1 (MQ=255)
   acGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGcc                                                                                                                                              <  1:366914/150‑1 (MQ=255)
    cgcgTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACCGTATGAGCGAAGAGATCATTTATTACGCCg                                                                                                                                             <  1:2141664/150‑1 (MQ=255)
     gcgTCCGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGa                                                                                                                                            <  2:1032889/150‑1 (MQ=255)
       gTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAg                                                                                                                                          <  2:2612307/150‑1 (MQ=255)
       gTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAg                                                                                                                                          <  2:528738/150‑1 (MQ=255)
          cGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGTGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTc                                                                                                                                       >  1:1805628/1‑150 (MQ=255)
           gggTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCa                                                                                                                                      >  2:1898262/1‑150 (MQ=255)
             gTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCAcc                                                                                                                                    >  2:1686855/1‑150 (MQ=255)
              tGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCAccc                                                                                                                                   >  1:1426501/1‑150 (MQ=255)
                aaaTCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGt                                                                                                                                 >  1:1313704/1‑150 (MQ=255)
                  aTCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTa                                                                                                                               <  1:2505285/150‑1 (MQ=255)
                       ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTCAcgcg                                                                                                                          >  2:830864/1‑150 (MQ=255)
                       ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgcg                                                                                                                          >  2:2887376/1‑150 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                        >  2:683085/1‑150 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                        <  2:1863327/150‑1 (MQ=255)
                         tGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                        <  1:516899/150‑1 (MQ=255)
                          ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                       >  2:3811712/1‑150 (MQ=255)
                             tAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGAttt                                                                                                                    <  1:936584/150‑1 (MQ=255)
                                   cTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAg                                                                                                              >  1:3441288/1‑150 (MQ=255)
                                    tACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGt                                                                                                             <  1:2276703/150‑1 (MQ=255)
                                              tGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAc                                                                                                   >  2:2838056/1‑150 (MQ=255)
                                                gACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCg                                                                                                 <  1:3436964/150‑1 (MQ=255)
                                                       gTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTg                                                                                          <  2:3612589/150‑1 (MQ=255)
                                                        tACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTgg                                                                                         >  1:1913764/1‑150 (MQ=255)
                                                        tACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTgg                                                                                         >  1:1644580/1‑150 (MQ=255)
                                                        tACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTgg                                                                                         >  1:1913930/1‑150 (MQ=255)
                                                         acatCGGTCAATCCTGAGTTAGCGTTGGGACAAATATACGGTGCCACACTGCTTGCTATCGGCCTCAGTATGAGCGAAGAGATGATTTATTTCGCCGAAGGTCAGCCGTTAACGCGTGATTTACGCTGTTACGGGTCAGCGAAAATTGGt                                                                                        <  2:1678571/150‑1 (MQ=255)
                                                         acacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGt                                                                                        >  2:1584297/1‑150 (MQ=255)
                                                              ggTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAt                                                                                   >  2:851032/1‑150 (MQ=255)
                                                              ggTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAt                                                                                   <  1:3482375/150‑1 (MQ=255)
                                                               gTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAtt                                                                                  <  2:3973165/150‑1 (MQ=255)
                                                                 cAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTcc                                                                                <  1:1825743/150‑1 (MQ=255)
                                                                   aTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgc                                                                              >  1:3145558/1‑150 (MQ=255)
                                                                   aTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgc                                                                              >  1:315651/1‑150 (MQ=255)
                                                                      cAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTg                                                                           <  1:2808717/150‑1 (MQ=255)
                                                                        gagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAt                                                                         <  1:617289/150‑1 (MQ=255)
                                                                         agTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAtt                                                                        >  1:1784809/1‑150 (MQ=255)
                                                                           ttAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTc                                                                      >  1:2361845/1‑150 (MQ=255)
                                                                            tAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTcc                                                                     >  2:3498741/1‑150 (MQ=255)
                                                                              gCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcgc                                                                   >  1:2886739/1‑150 (MQ=255)
                                                                               cGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcgcg                                                                  <  2:645133/150‑1 (MQ=255)
                                                                               cGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcgcg                                                                  <  1:1755072/150‑1 (MQ=255)
                                                                                gTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc                                                                 >  2:2031484/1‑150 (MQ=255)
                                                                                gTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc                                                                 >  1:2151554/1‑150 (MQ=255)
                                                                                 ttGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCt                                                                <  2:787835/150‑1 (MQ=255)
                                                                                 ttGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAGTCCGCGTGATTTCCGCGCt                                                                <  2:181512/150‑1 (MQ=255)
                                                                                  tGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCtg                                                               >  2:1763381/1‑150 (MQ=255)
                                                                                            cTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGcc                                                     >  2:3395319/1‑150 (MQ=255)
                                                                                             tACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCg                                                    <  1:3220965/150‑1 (MQ=255)
                                                                                             tACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCg                                                    >  2:3023496/1‑150 (MQ=255)
                                                                                             tACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCg                                                    <  1:4006072/150‑1 (MQ=255)
                                                                                              aCGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGa                                                   <  1:3535734/150‑1 (MQ=255)
                                                                                                ggTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                                 >  2:3844528/1‑150 (MQ=255)
                                                                                                ggTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                                 >  2:3844609/1‑150 (MQ=255)
                                                                                                  tGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcga                                               <  1:3311502/150‑1 (MQ=255)
                                                                                                   gCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGcgac                                              <  2:45486/150‑1 (MQ=255)
                                                                                                     cACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacga                                            >  1:768384/1‑150 (MQ=255)
                                                                                                     cACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacga                                            >  1:4039879/1‑150 (MQ=255)
                                                                                                     cACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacga                                            <  2:3301831/150‑1 (MQ=255)
                                                                                                        ccTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaa                                         >  1:2105186/1‑150 (MQ=255)
                                                                                                         cTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaaa                                        >  2:3078884/1‑150 (MQ=255)
                                                                                                          tGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAg                                       <  2:559409/150‑1 (MQ=255)
                                                                                                          tGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAg                                       <  1:3946470/150‑1 (MQ=255)
                                                                                                            cGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTc                                     <  1:2767129/150‑1 (MQ=255)
                                                                                                             gTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCg                                    <  2:2286697/150‑1 (MQ=255)
                                                                                                             gTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCg                                    >  2:4054997/1‑150 (MQ=255)
                                                                                                                cTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                 <  1:3749477/150‑1 (MQ=255)
                                                                                                                 tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                                <  1:1973454/150‑1 (MQ=255)
                                                                                                                    cGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGtt                             <  1:3448636/150‑1 (MQ=255)
                                                                                                                    cGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGtt                             <  1:1121222/150‑1 (MQ=255)
                                                                                                                     ggCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTc                            <  1:1249994/150‑1 (MQ=255)
                                                                                                                     ggCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTc                            <  1:1250223/150‑1 (MQ=255)
                                                                                                                     ggCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTc                            >  1:1585055/1‑150 (MQ=255)
                                                                                                                     ggCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTc                            <  1:3495082/150‑1 (MQ=255)
                                                                                                                      gCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCg                           <  1:2481679/150‑1 (MQ=255)
                                                                                                                      gCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAGTCGGCCCGTTCg                           <  2:926806/150‑1 (MQ=255)
                                                                                                                          caGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGc                       >  1:1556020/1‑150 (MQ=255)
                                                                                                                          caGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGc                       >  2:1852378/1‑150 (MQ=255)
                                                                                                                          caGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGc                       >  2:1239656/1‑150 (MQ=255)
                                                                                                                            gTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGa                     <  2:2554801/150‑1 (MQ=255)
                                                                                                                               tGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                  >  2:2060874/1‑150 (MQ=255)
                                                                                                                               tGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                  <  1:2446027/150‑1 (MQ=255)
                                                                                                                                 aGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCg                >  1:1885088/1‑150 (MQ=255)
                                                                                                                                   cGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt              >  2:2270043/1‑150 (MQ=255)
                                                                                                                                   cGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt              <  1:1694940/150‑1 (MQ=255)
                                                                                                                                   cGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt              <  1:2993091/150‑1 (MQ=255)
                                                                                                                                   cGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt              >  1:2054517/1‑150 (MQ=255)
                                                                                                                                    gAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc             >  2:3101238/1‑150 (MQ=255)
                                                                                                                                    gAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc             >  2:2086460/1‑150 (MQ=255)
                                                                                                                                     aaGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtct            >  2:121158/1‑150 (MQ=255)
                                                                                                                                      agagATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtctc           >  1:3832161/1‑150 (MQ=255)
                                                                                                                                       gagaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg          >  2:1747151/1‑150 (MQ=255)
                                                                                                                                         gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGa        >  2:352006/1‑150 (MQ=255)
                                                                                                                                           tCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaaa      >  1:3867919/1‑150 (MQ=255)
                                                                                                                                             aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc    >  2:1367459/1‑150 (MQ=255)
                                                                                                                                             aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc    >  1:4147560/1‑150 (MQ=255)
                                                                                                                                             aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc    >  1:1672106/1‑150 (MQ=255)
                                                                                                                                             aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc    <  2:795911/150‑1 (MQ=255)
                                                                                                                                               ttattaCGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCgg  <  1:622662/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGG  >  CP009273/3017138‑3017430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  CP009273/3017138‑3017433
                                                                                                                                                   |                                                                                                                                                    
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973074.1361216/1‑150 (MQ=60)
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAC                                                                                                                                                    >  SRR19973074.341654/1‑150 (MQ=60)
 ACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACG                                                                                                                                                   <  SRR19973074.4060350/150‑1 (MQ=60)
   ACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCC                                                                                                                                                 <  SRR19973074.4158943/150‑1 (MQ=60)
   ACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCC                                                                                                                                                 <  SRR19973074.367591/150‑1 (MQ=60)
    CGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACCGTATGAGCGAAGAGATCATTTATTACGCCG                                                                                                                                                <  SRR19973074.2145683/150‑1 (MQ=60)
    CGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACCGTATGAGCGAAGAGATCATTTATTACGCCG                                                                                                                                                <  SRR19973074.2145684/150‑1 (MQ=60)
          CGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGTGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTC                                                                                                                                          >  SRR19973074.1809047/1‑150 (MQ=60)
          CGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGTGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTC                                                                                                                                          >  SRR19973074.1809063/1‑150 (MQ=60)
          CGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTA                                                                                                                                          <  SRR19973074.183457/150‑1 (MQ=60)
              TGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCC                                                                                                                                      >  SRR19973074.1429140/1‑150 (MQ=60)
               GAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCG                                                                                                                                     <  SRR19973074.3507732/150‑1 (MQ=60)
               GAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCG                                                                                                                                     <  SRR19973074.3508007/150‑1 (MQ=60)
                AAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGT                                                                                                                                    >  SRR19973074.1316172/1‑150 (MQ=60)
                AAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTAAATCAAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGATATCATTTATTACGCCGAAGTTCACCCGT                                                                                                                                    >  SRR19973074.2494923/1‑150 (MQ=60)
                 AATCCGCCTTGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTT                                                                                                                                   >  SRR19973074.3333668/1‑150 (MQ=60)
                  ATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTA                                                                                                                                  <  SRR19973074.2509948/150‑1 (MQ=60)
                       CCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCG                                                                                                                             <  SRR19973074.3673689/150‑1 (MQ=60)
                        CTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGT                                                                                                                            >  SRR19973074.2100217/1‑150 (MQ=60)
                         TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           <  SRR19973074.2158420/150‑1 (MQ=60)
                         TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           <  SRR19973074.517930/150‑1 (MQ=60)
                         TGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTG                                                                                                                           >  SRR19973074.1142530/1‑150 (MQ=60)
                           GATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGAT                                                                                                                         >  SRR19973074.182178/1‑150 (MQ=60)
                             TAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTT                                                                                                                       <  SRR19973074.938367/150‑1 (MQ=60)
                              AAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTA                                                                                                                      >  SRR19973074.536556/1‑150 (MQ=60)
                                ATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACG                                                                                                                    <  SRR19973074.998929/150‑1 (MQ=60)
                                   CTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAG                                                                                                                 >  SRR19973074.3447623/1‑150 (MQ=60)
                                    TACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGT                                                                                                                >  SRR19973074.2459320/1‑150 (MQ=60)
                                    TACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGT                                                                                                                <  SRR19973074.2280979/150‑1 (MQ=60)
                                    TACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGT                                                                                                                <  SRR19973074.2280977/150‑1 (MQ=60)
                                        CCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACG                                                                                                            >  SRR19973074.1912783/1‑150 (MQ=60)
                                        CCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACG                                                                                                            >  SRR19973074.1912784/1‑150 (MQ=60)
                                                GACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCG                                                                                                    <  SRR19973074.3443296/150‑1 (MQ=60)
                                                GACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCG                                                                                                    <  SRR19973074.3443367/150‑1 (MQ=60)
                                                GACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCG                                                                                                    <  SRR19973074.4035261/150‑1 (MQ=60)
                                                   TGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAA                                                                                                 >  SRR19973074.3494606/1‑150 (MQ=60)
                                                        TACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGG                                                                                            >  SRR19973074.1917602/1‑150 (MQ=60)
                                                        TACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGG                                                                                            >  SRR19973074.1917567/1‑150 (MQ=60)
                                                        TACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGG                                                                                            >  SRR19973074.1647692/1‑150 (MQ=60)
                                                        TACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGG                                                                                            >  SRR19973074.1917436/1‑150 (MQ=60)
                                                           ACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGA                                                                                         <  SRR19973074.3249266/150‑1 (MQ=60)
                                                              GGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAT                                                                                      <  SRR19973074.2905030/150‑1 (MQ=60)
                                                              GGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAT                                                                                      <  SRR19973074.3488766/150‑1 (MQ=60)
                                                                 CAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCC                                                                                   <  SRR19973074.1829228/150‑1 (MQ=60)
                                                                  AATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCG                                                                                  >  SRR19973074.315197/1‑150 (MQ=60)
                                                                   ATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGC                                                                                 >  SRR19973074.316230/1‑150 (MQ=60)
                                                                   ATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGC                                                                                 >  SRR19973074.3151332/1‑150 (MQ=60)
                                                                   ATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGC                                                                                 <  SRR19973074.824566/150‑1 (MQ=60)
                                                                      CAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTG                                                                              <  SRR19973074.2813905/150‑1 (MQ=60)
                                                                        GAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAT                                                                            <  SRR19973074.618457/150‑1 (MQ=60)
                                                                        GAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAT                                                                            <  SRR19973074.618571/150‑1 (MQ=60)
                                                                         AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           >  SRR19973074.1788190/1‑150 (MQ=60)
                                                                         AGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGGTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATT                                                                           >  SRR19973074.1788166/1‑150 (MQ=60)
                                                                           TTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTC                                                                         >  SRR19973074.1591535/1‑150 (MQ=60)
                                                                           TTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTC                                                                         >  SRR19973074.2366240/1‑150 (MQ=60)
                                                                             AGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCG                                                                       >  SRR19973074.2915340/1‑150 (MQ=60)
                                                                              GCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGC                                                                      >  SRR19973074.2892094/1‑150 (MQ=60)
                                                                               CGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCG                                                                     <  SRR19973074.2457242/150‑1 (MQ=60)
                                                                               CGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCG                                                                     <  SRR19973074.1758341/150‑1 (MQ=60)
                                                                               CGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCG                                                                     <  SRR19973074.1758340/150‑1 (MQ=60)
                                                                                GTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGC                                                                    >  SRR19973074.2155606/1‑150 (MQ=60)
                                                                                  TGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTG                                                                  >  SRR19973074.2114809/1‑150 (MQ=60)
                                                                                   GGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAGATTGGTGACATTCCGCGTGATTTCCGCGCTGT                                                                 >  SRR19973074.1919141/1‑150 (MQ=60)
                                                                                         AATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGT                                                           <  SRR19973074.1985288/150‑1 (MQ=60)
                                                                                           TCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGC                                                         <  SRR19973074.727217/150‑1 (MQ=60)
                                                                                             TACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCG                                                       <  SRR19973074.4013477/150‑1 (MQ=60)
                                                                                             TACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCG                                                       <  SRR19973074.3226915/150‑1 (MQ=60)
                                                                                              ACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGA                                                      <  SRR19973074.3542247/150‑1 (MQ=60)
                                                                                              ACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGA                                                      <  SRR19973074.1443121/150‑1 (MQ=60)
                                                                                                GGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGC                                                    <  SRR19973074.3484696/150‑1 (MQ=60)
                                                                                                  TGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGA                                                  <  SRR19973074.3317648/150‑1 (MQ=60)
                                                                                                     CACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGA                                               >  SRR19973074.769859/1‑150 (MQ=60)
                                                                                                     CACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGA                                               >  SRR19973074.4047347/1‑150 (MQ=60)
                                                                                                        CCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAA                                            >  SRR19973074.1587760/1‑150 (MQ=60)
                                                                                                        CCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAA                                            >  SRR19973074.2109129/1‑150 (MQ=60)
                                                                                                          TGCGTGCTATGGGGCACAGTAGGAGCGAAGAGATCATTTATTACGCCGAAGTTGACCAGTTAACGCGTGATTTTCGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGTTTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAT                                          <  SRR19973074.3953616/150‑1 (MQ=60)
                                                                                                          TGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAG                                          <  SRR19973074.375533/150‑1 (MQ=60)
                                                                                                          TGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAG                                          <  SRR19973074.3953739/150‑1 (MQ=60)
                                                                                                            CGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTC                                        <  SRR19973074.2772215/150‑1 (MQ=60)
                                                                                                            CGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTC                                        <  SRR19973074.2772340/150‑1 (MQ=60)
                                                                                                              TGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGG                                      >  SRR19973074.2708720/1‑150 (MQ=60)
                                                                                                              TGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGG                                      >  SRR19973074.1258955/1‑150 (MQ=60)
                                                                                                              TGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAGTCGG                                      <  SRR19973074.2839135/150‑1 (MQ=60)
                                                                                                                CTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCC                                    <  SRR19973074.3756709/150‑1 (MQ=60)
                                                                                                                CTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCC                                    <  SRR19973074.3756372/150‑1 (MQ=60)
                                                                                                                 TATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCC                                   <  SRR19973074.1977212/150‑1 (MQ=60)
                                                                                                                  ATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCG                                  >  SRR19973074.430014/1‑150 (MQ=60)
                                                                                                                  ATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCG                                  >  SRR19973074.430066/1‑150 (MQ=60)
                                                                                                                    CGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTT                                <  SRR19973074.1123338/150‑1 (MQ=60)
                                                                                                                    CGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTT                                <  SRR19973074.3454976/150‑1 (MQ=60)
                                                                                                                    CGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTT                                <  SRR19973074.3454971/150‑1 (MQ=60)
                                                                                                                     GGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTC                               >  SRR19973074.1588067/1‑150 (MQ=60)
                                                                                                                     GGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTC                               <  SRR19973074.3501491/150‑1 (MQ=60)
                                                                                                                     GGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTC                               <  SRR19973074.1252391/150‑1 (MQ=60)
                                                                                                                     GGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTC                               <  SRR19973074.1252621/150‑1 (MQ=60)
                                                                                                                     GGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTC                               <  SRR19973074.1252394/150‑1 (MQ=60)
                                                                                                                      GCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCG                              <  SRR19973074.2486281/150‑1 (MQ=60)
                                                                                                                      GCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCG                              <  SRR19973074.1657866/150‑1 (MQ=60)
                                                                                                                          CAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGC                          >  SRR19973074.1558978/1‑150 (MQ=60)
                                                                                                                               TGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAT                     <  SRR19973074.2450555/150‑1 (MQ=60)
                                                                                                                               TGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAT                     <  SRR19973074.2448045/150‑1 (MQ=60)
                                                                                                                               TGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAT                     <  SRR19973074.3920317/150‑1 (MQ=60)
                                                                                                                                GAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATC                    <  SRR19973074.3217426/150‑1 (MQ=60)
                                                                                                                                 AGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCG                   >  SRR19973074.1888694/1‑150 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 <  SRR19973074.2998653/150‑1 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 <  SRR19973074.1698106/150‑1 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 <  SRR19973074.2998730/150‑1 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 <  SRR19973074.1698054/150‑1 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 <  SRR19973074.1698112/150‑1 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 >  SRR19973074.2058379/1‑150 (MQ=60)
                                                                                                                                   CGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCAGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAT                 >  SRR19973074.1446782/1‑150 (MQ=60)
                                                                                                                                      AGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTC              >  SRR19973074.3839145/1‑150 (MQ=60)
                                                                                                                                           TCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAA         >  SRR19973074.3874989/1‑150 (MQ=60)
                                                                                                                                           TCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAA         >  SRR19973074.3874950/1‑150 (MQ=60)
                                                                                                                                            CATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAT        <  SRR19973074.3970940/150‑1 (MQ=60)
                                                                                                                                             ATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATC       >  SRR19973074.4155247/1‑150 (MQ=60)
                                                                                                                                             ATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATC       >  SRR19973074.4155201/1‑150 (MQ=60)
                                                                                                                                             ATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATC       >  SRR19973074.1675250/1‑150 (MQ=60)
                                                                                                                                               TTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGG     <  SRR19973074.2115945/150‑1 (MQ=60)
                                                                                                                                               TTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGG     <  SRR19973074.623833/150‑1 (MQ=60)
                                                                                                                                                  TTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  <  SRR19973074.4059279/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
AACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGT  >  CP009273/3017138‑3017433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑