Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,489,397 C→A 91.8% F676L (TTC→TTA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,489,3970CA91.8% 739.4 / 53.5 244F676L (TTC→TTA) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base C (11/9);  new base A (100/122);  total (112/132)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 245 total aligned reads displayed.

CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489250‑3489546
                                                                                                                                                   |                                                                                                                                                     
cGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAgg                                                                                                                                                     >  2:1718414/1‑150 (MQ=255)
    cATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtc                                                                                                                                                 <  1:1898101/150‑1 (MQ=255)
     aTGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtct                                                                                                                                                <  2:2858357/150‑1 (MQ=255)
       gCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTcc                                                                                                                                              >  2:3201805/1‑150 (MQ=255)
       gCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTcc                                                                                                                                              <  1:1420179/150‑1 (MQ=255)
        ccTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCg                                                                                                                                             <  1:277889/150‑1 (MQ=255)
          tATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGc                                                                                                                                           >  2:2638198/1‑150 (MQ=255)
           aTGCGACAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                          <  2:2794627/150‑1 (MQ=255)
           aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                          <  2:2277550/150‑1 (MQ=255)
           aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                          <  2:872437/150‑1 (MQ=255)
           aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTGAGGTGTCTCCGGCt                                                                                                                                          >  2:2935367/1‑150 (MQ=255)
            tGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTg                                                                                                                                         <  2:411910/150‑1 (MQ=255)
             gCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGt                                                                                                                                        >  1:1835831/1‑150 (MQ=255)
                    cACTGCGTATGGCGACAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTg                                                                                                                                 <  2:2355424/150‑1 (MQ=255)
                    cACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAACGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTg                                                                                                                                 <  2:1196628/150‑1 (MQ=255)
                       tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                              <  1:964982/150‑1 (MQ=255)
                       tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                              >  2:3594150/1‑150 (MQ=255)
                       tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                              >  2:706575/1‑150 (MQ=255)
                        gCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATg                                                                                                                             <  1:268215/150‑1 (MQ=255)
                        gCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATg                                                                                                                             <  1:3342135/150‑1 (MQ=255)
                        gCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAAGGACGTTAGGTGTCTCCGGCTGTACCCGTGAATg                                                                                                                             >  2:3747923/1‑150 (MQ=255)
                         cGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGt                                                                                                                            <  1:1323385/150‑1 (MQ=255)
                          gTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGtt                                                                                                                           >  1:3688505/1‑150 (MQ=255)
                            aTGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCa                                                                                                                         >  2:2865297/1‑150 (MQ=255)
                              ggCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGa                                                                                                                       >  1:1284639/1‑150 (MQ=255)
                                 gAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGc                                                                                                                    >  1:2196108/1‑150 (MQ=255)
                                   aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                                  <  2:184160/150‑1 (MQ=255)
                                   aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCTGAAGCTc                                                                                                                  >  1:3590290/1‑150 (MQ=255)
                                   aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                                  >  2:571220/1‑150 (MQ=255)
                                      ccTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAgg                                                                                                               <  2:239298/150‑1 (MQ=255)
                                         gCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTa                                                                                                            <  2:1933368/150‑1 (MQ=255)
                                           gTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTaaa                                                                                                          >  1:3416118/1‑150 (MQ=255)
                                               gTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACg                                                                                                      <  1:1170084/150‑1 (MQ=255)
                                               gTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACg                                                                                                      <  2:406947/150‑1 (MQ=255)
                                                 aGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTg                                                                                                    <  1:3752633/150‑1 (MQ=255)
                                                  gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                                   <  1:2117165/150‑1 (MQ=255)
                                                    aCCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGt                                                                                                 >  2:2230666/1‑150 (MQ=255)
                                                       tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatt                                                                                              >  2:2246191/1‑150 (MQ=255)
                                                       tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatt                                                                                              >  1:2261654/1‑150 (MQ=255)
                                                        ggTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatta                                                                                             >  2:1691970/1‑150 (MQ=255)
                                                           gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                          <  1:2725418/150‑1 (MQ=255)
                                                           gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                          <  1:894002/150‑1 (MQ=255)
                                                            ccGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGc                                                                                         <  1:2918442/150‑1 (MQ=255)
                                                                 aCGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTg                                                                                    >  1:2743069/1‑150 (MQ=255)
                                                                  cGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGa                                                                                   >  2:652671/1‑150 (MQ=255)
                                                                  cGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGa                                                                                   <  2:1340975/150‑1 (MQ=255)
                                                                      gTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaaaa                                                                               >  2:405577/1‑150 (MQ=255)
                                                                       ttGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAg                                                                              <  2:3005822/150‑1 (MQ=255)
                                                                        tGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAgg                                                                             <  1:2006082/150‑1 (MQ=255)
                                                                        tGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAgg                                                                             <  1:647372/150‑1 (MQ=255)
                                                                         gtcGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGc                                                                            <  2:619193/148‑1 (MQ=255)
                                                                         ggCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGc                                                                            >  1:2512063/1‑150 (MQ=255)
                                                                         ggCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGc                                                                            <  1:3812188/150‑1 (MQ=255)
                                                                            gACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTgg                                                                         <  2:2549903/150‑1 (MQ=255)
                                                                               aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                      <  1:1226526/150‑1 (MQ=255)
                                                                                    cGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTa                                                                 <  1:3198947/150‑1 (MQ=255)
                                                                                       ccTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGt                                                              >  1:4164423/1‑150 (MQ=255)
                                                                                         tCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGttt                                                            <  2:4250822/150‑1 (MQ=255)
                                                                                         tCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGttt                                                            <  2:2138865/150‑1 (MQ=255)
                                                                                          cGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTg                                                           <  1:983461/150‑1 (MQ=255)
                                                                                          cGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTg                                                           <  1:2835871/150‑1 (MQ=255)
                                                                                           ggCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGc                                                          >  1:3989183/1‑150 (MQ=255)
                                                                                            gCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAACTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCg                                                         <  2:510935/150‑1 (MQ=255)
                                                                                                tgAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTaa                                                     <  2:3407495/149‑1 (MQ=255)
                                                                                                  aaCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcg                                                   >  1:806909/1‑150 (MQ=255)
                                                                                                    cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                                 <  1:2310086/150‑1 (MQ=255)
                                                                                                    cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                                 >  1:3778709/1‑150 (MQ=255)
                                                                                                      ggAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggcgg                                               <  2:3088717/150‑1 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                              >  1:4276577/1‑150 (MQ=255)
                                                                                                        aaaaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCa                                             >  2:2292206/1‑150 (MQ=255)
                                                                                                           aCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGa                                          <  1:3472034/150‑1 (MQ=255)
                                                                                                           aCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGa                                          <  1:3473266/150‑1 (MQ=255)
                                                                                                             cGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGaaa                                        >  1:1605414/1‑150 (MQ=255)
                                                                                                                tACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcg                                     >  2:2147182/1‑150 (MQ=255)
                                                                                                                 aCAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcgc                                    >  1:697088/1‑150 (MQ=255)
                                                                                                                   aaaGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGt                                  >  2:1561278/1‑150 (MQ=255)
                                                                                                                   aaaGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGt                                  >  2:1170460/1‑150 (MQ=255)
                                                                                                                      ggCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAt                               <  1:2033487/150‑1 (MQ=255)
                                                                                                                       gCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATg                              <  1:1064403/150‑1 (MQ=255)
                                                                                                                          tCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCgg                           <  1:1517874/150‑1 (MQ=255)
                                                                                                                          tCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCgg                           >  2:3889798/1‑150 (MQ=255)
                                                                                                                            cGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTACCGGCGGCATGAAACCGCGTCATGCGGAt                         >  2:4176556/1‑150 (MQ=255)
                                                                                                                            cGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGAt                         >  1:3954395/1‑150 (MQ=255)
                                                                                                                             gTACGCCGCACCAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                        <  2:2325501/150‑1 (MQ=255)
                                                                                                                             gTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                        <  2:2397656/150‑1 (MQ=255)
                                                                                                                             gTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                        <  2:3017102/150‑1 (MQ=255)
                                                                                                                              tACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATct                       <  1:3900319/150‑1 (MQ=255)
                                                                                                                              tACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATct                       <  1:1687979/150‑1 (MQ=255)
                                                                                                                                cgccgcACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgc                     >  2:2908025/1‑150 (MQ=255)
                                                                                                                                cgccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgc                     >  1:3643025/1‑150 (MQ=255)
                                                                                                                                   cgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTgg                  <  2:3329818/150‑1 (MQ=255)
                                                                                                                                     cacaAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcg                <  2:1807631/150‑1 (MQ=255)
                                                                                                                                     cacaAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcg                >  1:848394/1‑150 (MQ=255)
                                                                                                                                      acaAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcgg               <  2:3984765/150‑1 (MQ=255)
                                                                                                                                       caAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcggc              <  2:3179202/150‑1 (MQ=255)
                                                                                                                                         aaaTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGcggcgg            >  1:3601946/1‑150 (MQ=255)
                                                                                                                                             gAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGatat        <  1:2901437/150‑1 (MQ=255)
                                                                                                                                              aaGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatc       <  1:2346258/150‑1 (MQ=255)
                                                                                                                                               aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg      <  1:4027021/150‑1 (MQ=255)
                                                                                                                                                gTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcga     >  1:1002267/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489250‑3489546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489254‑3489546
                                                                                                                                               |                                                                                                                                                     
CATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTC                                                                                                                                                 <  SRR19973079.1908203/150‑1 (MQ=60)
CATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTC                                                                                                                                                 <  SRR19973079.1908209/150‑1 (MQ=60)
 ATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCT                                                                                                                                                >  SRR19973079.1543005/1‑150 (MQ=60)
  TGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTC                                                                                                                                               >  SRR19973079.2903568/1‑150 (MQ=60)
   GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973079.1427885/150‑1 (MQ=60)
   GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973079.3630490/150‑1 (MQ=60)
    CCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTGGGTGTCTCCG                                                                                                                                             >  SRR19973079.2024621/1‑150 (MQ=60)
    CCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCG                                                                                                                                             >  SRR19973079.636172/1‑150 (MQ=60)
    CCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCG                                                                                                                                             <  SRR19973079.279615/150‑1 (MQ=60)
     CTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGG                                                                                                                                            >  SRR19973079.2159428/1‑150 (MQ=60)
      TATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGC                                                                                                                                           >  SRR19973079.4171553/1‑150 (MQ=60)
      TATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGC                                                                                                                                           >  SRR19973079.1666021/1‑150 (MQ=60)
         GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGT                                                                                                                                        >  SRR19973079.1845491/1‑150 (MQ=60)
         GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGT                                                                                                                                        >  SRR19973079.1845148/1‑150 (MQ=60)
         GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGT                                                                                                                                        >  SRR19973079.1302792/1‑150 (MQ=60)
         GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGT                                                                                                                                        <  SRR19973079.3792377/150‑1 (MQ=60)
         GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGT                                                                                                                                        <  SRR19973079.2640747/150‑1 (MQ=60)
          CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       >  SRR19973079.3911016/1‑150 (MQ=60)
          AGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       <  SRR19973079.2355393/150‑1 (MQ=60)
              TGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCG                                                                                                                                   <  SRR19973079.1411155/150‑1 (MQ=60)
              AGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCG                                                                                                                                   >  SRR19973079.1525904/1‑150 (MQ=60)
                   TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973079.970490/150‑1 (MQ=60)
                   TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973079.506758/150‑1 (MQ=60)
                    GCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATG                                                                                                                             <  SRR19973079.269915/150‑1 (MQ=60)
                    GCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATG                                                                                                                             <  SRR19973079.3359280/150‑1 (MQ=60)
                    GCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATG                                                                                                                             <  SRR19973079.2286739/150‑1 (MQ=60)
                     CGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGT                                                                                                                            <  SRR19973079.1330842/150‑1 (MQ=60)
                      GTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTT                                                                                                                           <  SRR19973079.1142128/150‑1 (MQ=60)
                      GTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTT                                                                                                                           >  SRR19973079.3707321/1‑150 (MQ=60)
                       TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          <  SRR19973079.1291795/150‑1 (MQ=60)
                       TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          <  SRR19973079.3693869/150‑1 (MQ=60)
                        ATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCA                                                                                                                         <  SRR19973079.2239062/150‑1 (MQ=60)
                        ATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCA                                                                                                                         <  SRR19973079.3807808/150‑1 (MQ=60)
                          GGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGA                                                                                                                       >  SRR19973079.1291955/1‑150 (MQ=60)
                             GAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGC                                                                                                                    >  SRR19973079.2207404/1‑150 (MQ=60)
                              AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                   >  SRR19973079.2854449/1‑150 (MQ=60)
                              AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                   >  SRR19973079.3387444/1‑150 (MQ=60)
                              AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                   >  SRR19973079.3387915/1‑150 (MQ=60)
                               AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCTGAAGCTC                                                                                                                  >  SRR19973079.3608552/1‑150 (MQ=60)
                               AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTC                                                                                                                  <  SRR19973079.4005294/150‑1 (MQ=60)
                                AACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCA                                                                                                                 >  SRR19973079.2821403/1‑150 (MQ=60)
                                    TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973079.3935443/1‑150 (MQ=60)
                                    TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973079.165067/1‑150 (MQ=60)
                                      CGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAA                                                                                                           >  SRR19973079.361374/1‑150 (MQ=60)
                                       GTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAA                                                                                                          >  SRR19973079.1229443/1‑150 (MQ=60)
                                       GTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAA                                                                                                          >  SRR19973079.3433756/1‑150 (MQ=60)
                                        TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                         >  SRR19973079.372758/1‑150 (MQ=60)
                                        TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                         >  SRR19973079.372541/1‑150 (MQ=60)
                                        TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                         >  SRR19973079.278850/1‑150 (MQ=60)
                                          GGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAC                                                                                                       <  SRR19973079.724113/150‑1 (MQ=60)
                                          GGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAC                                                                                                       <  SRR19973079.724067/150‑1 (MQ=60)
                                           GTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTATAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACG                                                                                                      <  SRR19973079.975632/150‑1 (MQ=60)
                                           GTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACG                                                                                                      <  SRR19973079.1176821/150‑1 (MQ=60)
                                             AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    >  SRR19973079.890071/1‑150 (MQ=60)
                                             AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    <  SRR19973079.3771856/150‑1 (MQ=60)
                                              GCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGG                                                                                                   <  SRR19973079.2128127/150‑1 (MQ=60)
                                                   TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              >  SRR19973079.3810474/1‑150 (MQ=60)
                                                   TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              >  SRR19973079.2273384/1‑150 (MQ=60)
                                                     GTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTAT                                                                                            >  SRR19973079.94303/1‑150 (MQ=60)
                                                      TGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATC                                                                                           <  SRR19973079.2243997/150‑1 (MQ=60)
                                                       GCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCG                                                                                          <  SRR19973079.2739511/150‑1 (MQ=60)
                                                       GCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCG                                                                                          <  SRR19973079.899192/150‑1 (MQ=60)
                                                        CCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGC                                                                                         <  SRR19973079.2933101/150‑1 (MQ=60)
                                                        CCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGC                                                                                         <  SRR19973079.2933269/150‑1 (MQ=60)
                                                             ACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTG                                                                                    >  SRR19973079.4080887/1‑150 (MQ=60)
                                                             ACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTG                                                                                    >  SRR19973079.2757221/1‑150 (MQ=60)
                                                               GGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAA                                                                                  >  SRR19973079.1947078/1‑150 (MQ=60)
                                                                  GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                               >  SRR19973079.839835/1‑150 (MQ=60)
                                                                    TGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGG                                                                             <  SRR19973079.2016692/150‑1 (MQ=60)
                                                                    TGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGG                                                                             <  SRR19973079.651029/150‑1 (MQ=60)
                                                                     GGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGC                                                                            <  SRR19973079.3831697/150‑1 (MQ=60)
                                                                     GGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGC                                                                            >  SRR19973079.2524786/1‑150 (MQ=60)
                                                                           AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCATGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      >  SRR19973079.2507932/1‑150 (MQ=60)
                                                                           AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      <  SRR19973079.1233569/150‑1 (MQ=60)
                                                                           AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      >  SRR19973079.3514168/1‑150 (MQ=60)
                                                                             CGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTCAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCT                                                                    >  SRR19973079.2645906/1‑150 (MQ=60)
                                                                                CGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTA                                                                 <  SRR19973079.3215273/150‑1 (MQ=60)
                                                                                   CCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGT                                                              >  SRR19973079.4185554/1‑150 (MQ=60)
                                                                                      CGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTG                                                           <  SRR19973079.989025/150‑1 (MQ=60)
                                                                                      CGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTG                                                           <  SRR19973079.2850335/150‑1 (MQ=60)
                                                                                       GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                          <  SRR19973079.1024851/150‑1 (MQ=60)
                                                                                       GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                          >  SRR19973079.4011926/1‑150 (MQ=60)
                                                                                       GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                          >  SRR19973079.4009201/1‑150 (MQ=60)
                                                                                         CGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGG                                                        <  SRR19973079.2742008/150‑1 (MQ=60)
                                                                                           TGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAATTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTA                                                      <  SRR19973079.1492205/150‑1 (MQ=60)
                                                                                           TGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTA                                                      <  SRR19973079.1315215/150‑1 (MQ=60)
                                                                                           TGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTA                                                      <  SRR19973079.3172282/150‑1 (MQ=60)
                                                                                              AACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACG                                                   >  SRR19973079.811521/1‑150 (MQ=60)
                                                                                                CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                 <  SRR19973079.2322073/150‑1 (MQ=60)
                                                                                                CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                 <  SRR19973079.2322077/150‑1 (MQ=60)
                                                                                                CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                 >  SRR19973079.3798067/1‑150 (MQ=60)
                                                                                                   GAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGC                                              >  SRR19973079.4298184/1‑150 (MQ=60)
                                                                                                      AACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATG                                           >  SRR19973079.305382/1‑150 (MQ=60)
                                                                                                       ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          <  SRR19973079.3491169/150‑1 (MQ=60)
                                                                                                       ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          <  SRR19973079.197600/150‑1 (MQ=60)
                                                                                                       ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          <  SRR19973079.3598648/150‑1 (MQ=60)
                                                                                                       ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          <  SRR19973079.3489932/150‑1 (MQ=60)
                                                                                                       ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          <  SRR19973079.3490008/150‑1 (MQ=60)
                                                                                                        CCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAA                                         <  SRR19973079.3320114/150‑1 (MQ=60)
                                                                                                         CGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAA                                        >  SRR19973079.1614224/1‑150 (MQ=60)
                                                                                                          GCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAC                                       <  SRR19973079.3479263/150‑1 (MQ=60)
                                                                                                             ACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGC                                    >  SRR19973079.700869/1‑150 (MQ=60)
                                                                                                             ACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGC                                    >  SRR19973079.700868/1‑150 (MQ=60)
                                                                                                              CAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTTTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCG                                   >  SRR19973079.2075514/1‑150 (MQ=60)
                                                                                                                  GGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAT                               <  SRR19973079.2044183/150‑1 (MQ=60)
                                                                                                                   GCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATG                              <  SRR19973079.1070218/150‑1 (MQ=60)
                                                                                                                   GCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATG                              <  SRR19973079.4263927/150‑1 (MQ=60)
                                                                                                                      TCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGG                           <  SRR19973079.1526238/150‑1 (MQ=60)
                                                                                                                        CGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGAT                         >  SRR19973079.3974319/1‑150 (MQ=60)
                                                                                                                          TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973079.3920744/150‑1 (MQ=60)
                                                                                                                          TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973079.1697057/150‑1 (MQ=60)
                                                                                                                          TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973079.3920101/150‑1 (MQ=60)
                                                                                                                            CGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGC                     >  SRR19973079.3661495/1‑150 (MQ=60)
                                                                                                                            CGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGC                     >  SRR19973079.3661523/1‑150 (MQ=60)
                                                                                                                                 CACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCG                >  SRR19973079.853303/1‑150 (MQ=60)
                                                                                                                                 CACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCG                >  SRR19973079.854829/1‑150 (MQ=60)
                                                                                                                                     AAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGG            >  SRR19973079.3620237/1‑150 (MQ=60)
                                                                                                                                        TGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATA         <  SRR19973079.1181137/150‑1 (MQ=60)
                                                                                                                                         GAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATAT        <  SRR19973079.2916057/150‑1 (MQ=60)
                                                                                                                                          AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC       >  SRR19973079.2117750/1‑150 (MQ=60)
                                                                                                                                          AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC       <  SRR19973079.2358437/150‑1 (MQ=60)
                                                                                                                                           AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      <  SRR19973079.2710267/150‑1 (MQ=60)
                                                                                                                                           AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      <  SRR19973079.4047344/150‑1 (MQ=60)
                                                                                                                                            GTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGA     >  SRR19973079.1007924/1‑150 (MQ=60)
                                                                                                                                             TTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGAT    <  SRR19973079.1334075/150‑1 (MQ=60)
                                                                                                                                               CGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  <  SRR19973079.276242/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
CATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489254‑3489546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑