Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,489,397 C→A 100% F676L (TTC→TTA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,489,3970CA99.7% 1037.5 / ‑4.9 317F676L (TTC→TTA) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (155/159);  total (158/159)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGA  >  CP009273/3489250‑3489543
                                                                                                                                                   |                                                                                                                                                  
cGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAgg                                                                                                                                                  >  2:4368380/1‑150 (MQ=255)
cGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAgg                                                                                                                                                  <  2:2622476/150‑1 (MQ=255)
cGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAgg                                                                                                                                                  >  1:237238/1‑150 (MQ=255)
    cATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtc                                                                                                                                              >  2:1730081/1‑150 (MQ=255)
       gCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTcc                                                                                                                                           <  1:3225139/150‑1 (MQ=255)
        ccTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCg                                                                                                                                          <  2:233761/150‑1 (MQ=255)
          tATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGc                                                                                                                                        <  2:3071795/150‑1 (MQ=255)
           aTGCGAAAGCACTGCGTATGGAGAAAACCTGCGTGGGTAGCACCTCGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                       >  1:4851639/1‑150 (MQ=255)
            tGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTg                                                                                                                                      >  1:2637805/1‑150 (MQ=255)
             gCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGt                                                                                                                                     <  1:4916841/150‑1 (MQ=255)
              cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                    <  1:4998916/150‑1 (MQ=255)
                aaaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAcc                                                                                                                                  >  2:2803350/1‑150 (MQ=255)
                 aaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAccc                                                                                                                                 >  1:33405/1‑150 (MQ=255)
                 aaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAccc                                                                                                                                 >  2:3810501/1‑150 (MQ=255)
                  aGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCg                                                                                                                                <  2:2882386/150‑1 (MQ=255)
                       tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                           <  1:360998/150‑1 (MQ=255)
                       tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGAGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                           <  1:3486699/150‑1 (MQ=255)
                        gCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCCTCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATg                                                                                                                          >  2:141425/1‑150 (MQ=255)
                          gTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGtt                                                                                                                        <  1:5145710/150‑1 (MQ=255)
                           tATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTc                                                                                                                       <  2:3493453/150‑1 (MQ=255)
                           tATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTc                                                                                                                       <  2:2132189/150‑1 (MQ=255)
                           tATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTc                                                                                                                       >  1:2717575/1‑150 (MQ=255)
                            aTGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCa                                                                                                                      >  2:1179170/1‑150 (MQ=255)
                             tGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAg                                                                                                                     >  2:4597267/1‑150 (MQ=255)
                             tGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAg                                                                                                                     >  2:892386/1‑150 (MQ=255)
                             tGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAg                                                                                                                     >  2:189038/1‑150 (MQ=255)
                             tGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAg                                                                                                                     >  2:2085072/1‑150 (MQ=255)
                             tGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAg                                                                                                                     >  2:336586/1‑150 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                  >  2:3733803/1‑150 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                  >  1:3743827/1‑150 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                  >  1:4586947/1‑150 (MQ=255)
                                     aCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTTGAACTGGAAAACCGCTACTAAGGCATCCGTTCGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAg                                                                                                             <  1:4158788/150‑1 (MQ=255)
                                        tGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGt                                                                                                          >  1:997478/1‑150 (MQ=255)
                                         gCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTa                                                                                                         <  2:5040091/150‑1 (MQ=255)
                                            tGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAg                                                                                                      <  2:4268494/150‑1 (MQ=255)
                                             gggTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGa                                                                                                     <  2:2009062/150‑1 (MQ=255)
                                              ggTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCTGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAc                                                                                                    >  2:4379416/1‑150 (MQ=255)
                                              ggTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAc                                                                                                    >  1:1616295/1‑150 (MQ=255)
                                              ggTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAc                                                                                                    <  1:2994974/150‑1 (MQ=255)
                                                tAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGt                                                                                                  >  2:4151138/1‑150 (MQ=255)
                                                 aGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTg                                                                                                 <  1:1852662/150‑1 (MQ=255)
                                                 aGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTg                                                                                                 >  2:698406/1‑150 (MQ=255)
                                                  gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                                <  1:3634939/150‑1 (MQ=255)
                                                    aCCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGt                                                                                              <  1:3832536/150‑1 (MQ=255)
                                                     ccTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGta                                                                                             <  2:4576297/150‑1 (MQ=255)
                                                      cTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtat                                                                                            <  2:718581/150‑1 (MQ=255)
                                                       tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatt                                                                                           <  1:1664498/150‑1 (MQ=255)
                                                       tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatt                                                                                           <  1:1347787/150‑1 (MQ=255)
                                                              gCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCa                                                                                    >  1:1185165/1‑150 (MQ=255)
                                                               cTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAc                                                                                   >  1:3285631/1‑150 (MQ=255)
                                                                tACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACt                                                                                  <  2:3798056/150‑1 (MQ=255)
                                                                tACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACt                                                                                  >  1:1044439/1‑150 (MQ=255)
                                                                 aCGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTg                                                                                 <  2:2900424/150‑1 (MQ=255)
                                                                 aCGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTg                                                                                 >  1:1103915/1‑150 (MQ=255)
                                                                      gTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaaaa                                                                            <  1:3162405/150‑1 (MQ=255)
                                                                      gTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaaaa                                                                            <  2:758354/150‑1 (MQ=255)
                                                                           cGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTg                                                                       <  2:2461966/150‑1 (MQ=255)
                                                                              cAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGaa                                                                    <  2:4105496/150‑1 (MQ=255)
                                                                                gCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAcc                                                                  <  1:2194177/150‑1 (MQ=255)
                                                                                   tCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGt                                                               <  2:512188/150‑1 (MQ=255)
                                                                                    cGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTa                                                              <  2:4471523/150‑1 (MQ=255)
                                                                                     ggCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTAt                                                             <  2:3046914/150‑1 (MQ=255)
                                                                                              gTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGt                                                    <  2:3563340/150‑1 (MQ=255)
                                                                                                 gAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAc                                                 <  1:4046681/150‑1 (MQ=255)
                                                                                                   aCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcgg                                               >  1:436906/1‑150 (MQ=255)
                                                                                                   aCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcgg                                               >  1:1940993/1‑150 (MQ=255)
                                                                                                        aaaaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCa                                          <  1:3485648/150‑1 (MQ=255)
                                                                                                          aaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATg                                        >  1:2573130/1‑150 (MQ=255)
                                                                                                          aaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATg                                        <  2:4681107/150‑1 (MQ=255)
                                                                                                           aCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGa                                       >  2:1635124/1‑150 (MQ=255)
                                                                                                           aCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGa                                       >  1:3973145/1‑150 (MQ=255)
                                                                                                            ccGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAACCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGaa                                      <  1:1223269/150‑1 (MQ=255)
                                                                                                             cGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGaaa                                     >  2:3560196/1‑150 (MQ=255)
                                                                                                              gCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAc                                    >  2:2277098/1‑150 (MQ=255)
                                                                                                               cTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAcc                                   <  2:634429/150‑1 (MQ=255)
                                                                                                                tACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcg                                  <  1:4706381/150‑1 (MQ=255)
                                                                                                                tACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcg                                  >  2:917689/1‑150 (MQ=255)
                                                                                                                tACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcg                                  >  1:2775200/1‑150 (MQ=255)
                                                                                                                      ggCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAt                            <  2:3249118/150‑1 (MQ=255)
                                                                                                                      ggCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAt                            >  2:4901071/1‑150 (MQ=255)
                                                                                                                        cATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGc                          >  2:1482547/1‑150 (MQ=255)
                                                                                                                        cATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGc                          >  2:2551547/1‑150 (MQ=255)
                                                                                                                           ccGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGa                       >  1:1014826/1‑150 (MQ=255)
                                                                                                                             gTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                     >  1:2879119/1‑150 (MQ=255)
                                                                                                                              tACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATct                    <  1:84937/150‑1 (MQ=255)
                                                                                                                               aCGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctg                   <  2:1696092/150‑1 (MQ=255)
                                                                                                                                 gccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgct                 <  2:1103915/150‑1 (MQ=255)
                                                                                                                                  ccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGa                       >  1:452941/1‑143 (MQ=255)
                                                                                                                                  ccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgctg                <  2:3900996/150‑1 (MQ=255)
                                                                                                                                   cgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTgg               <  1:2448058/150‑1 (MQ=255)
                                                                                                                                   cgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTgg               >  2:3125898/1‑150 (MQ=255)
                                                                                                                                      accacaTGAAGTTAGGTGTCTCCGGCTGGACCCGTGCATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAACAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcgg            <  2:4851639/145‑1 (MQ=255)
                                                                                                                                       caAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTCTCGCCACTGAAAAAGGCTGGAACCTGTATGTGTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcggc           <  2:3018601/150‑1 (MQ=255)
                                                                                                                                       caAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcggc           <  2:126767/150‑1 (MQ=255)
                                                                                                                                         aaaTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGcggcgg         <  2:5163087/150‑1 (MQ=255)
                                                                                                                                           aTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGat       <  1:687458/150‑1 (MQ=255)
                                                                                                                                               aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg   >  1:1268643/1‑150 (MQ=255)
                                                                                                                                               aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg   >  1:4134518/1‑150 (MQ=255)
                                                                                                                                               aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAAGTGGGTATTATCTCCACTGAAAAAGGCTGGAACATGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg   >  1:3210993/1‑150 (MQ=255)
                                                                                                                                                gTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcga  >  1:3117077/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGA  >  CP009273/3489250‑3489543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489250‑3489546
                                                                                                                                                   |                                                                                                                                                     
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGG                                                                                                                                                     <  SRR19973063.1725246/150‑1 (MQ=60)
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGG                                                                                                                                                     >  SRR19973063.237952/1‑150 (MQ=60)
 GGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGT                                                                                                                                                    >  SRR19973063.3236565/1‑150 (MQ=60)
   TCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGT                                                                                                                                                  <  SRR19973063.4546079/150‑1 (MQ=60)
     ATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCT                                                                                                                                                >  SRR19973063.1459781/1‑150 (MQ=60)
       GCCTATGCGAAAGCACTGCGTATGGCTAAAACCTGCGTGGGTAGCACCTGGTGCCGCTTCGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTTGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973063.4998086/150‑1 (MQ=60)
       GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973063.2551424/150‑1 (MQ=60)
       GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              >  SRR19973063.2754375/1‑150 (MQ=60)
       GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973063.3235501/150‑1 (MQ=60)
       GCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGGAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCC                                                                                                                                              <  SRR19973063.4999677/150‑1 (MQ=60)
           ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                          >  SRR19973063.753998/1‑150 (MQ=60)
           ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                          <  SRR19973063.3858783/150‑1 (MQ=60)
           ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                          >  SRR19973063.2455124/1‑150 (MQ=60)
           ATGCGAAAGCACTGCGTATGGAGAAAACCTGCGTGGGTAGCACCTCGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                          >  SRR19973063.4867263/1‑150 (MQ=60)
            TGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTG                                                                                                                                         >  SRR19973063.2646226/1‑150 (MQ=60)
             GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGT                                                                                                                                        <  SRR19973063.4932717/150‑1 (MQ=60)
             GCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGT                                                                                                                                        <  SRR19973063.2664080/150‑1 (MQ=60)
              CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       <  SRR19973063.5015060/150‑1 (MQ=60)
               GAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAC                                                                                                                                      <  SRR19973063.645611/150‑1 (MQ=60)
                 AAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCC                                                                                                                                    <  SRR19973063.1200250/150‑1 (MQ=60)
                 AAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCC                                                                                                                                    >  SRR19973063.33536/1‑150 (MQ=60)
                  AGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCG                                                                                                                                   <  SRR19973063.4332346/150‑1 (MQ=60)
                    CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                 >  SRR19973063.1429229/1‑150 (MQ=60)
                     ACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGGACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGA                                                                                                                                >  SRR19973063.3174739/1‑150 (MQ=60)
                     ACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGGACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGA                                                                                                                                >  SRR19973063.3174923/1‑150 (MQ=60)
                     ACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGA                                                                                                                                >  SRR19973063.2150928/1‑150 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973063.362036/150‑1 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973063.362055/150‑1 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCAGGCTGTACCCGTGAAT                                                                                                                              >  SRR19973063.889693/1‑150 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGAGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973063.3497839/150‑1 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGAGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973063.3497842/150‑1 (MQ=60)
                       TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGAGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              <  SRR19973063.3497840/150‑1 (MQ=60)
                          GTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTT                                                                                                                           <  SRR19973063.5162274/150‑1 (MQ=60)
                          GTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTT                                                                                                                           >  SRR19973063.513687/1‑150 (MQ=60)
                           TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          >  SRR19973063.1630928/1‑150 (MQ=60)
                           TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          >  SRR19973063.2725713/1‑150 (MQ=60)
                           TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          >  SRR19973063.2726352/1‑150 (MQ=60)
                             TGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAG                                                                                                                        <  SRR19973063.4667997/150‑1 (MQ=60)
                             TGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAG                                                                                                                        <  SRR19973063.5005473/150‑1 (MQ=60)
                                CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                     >  SRR19973063.3845292/1‑150 (MQ=60)
                                CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                     >  SRR19973063.3755789/1‑150 (MQ=60)
                                CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                     <  SRR19973063.4177444/150‑1 (MQ=60)
                                CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                     >  SRR19973063.4601624/1‑150 (MQ=60)
                                CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                     >  SRR19973063.2794916/1‑150 (MQ=60)
                                 GAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGC                                                                                                                    >  SRR19973063.3451236/1‑150 (MQ=60)
                                 GAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGC                                                                                                                    >  SRR19973063.3451204/1‑150 (MQ=60)
                                  AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                   <  SRR19973063.3799653/150‑1 (MQ=60)
                                   AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTC                                                                                                                  >  SRR19973063.1342553/1‑150 (MQ=60)
                                     ACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTTGAACTGGAAAACCGCTACTAAGGCATCCGTTCGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAG                                                                                                                <  SRR19973063.4172089/150‑1 (MQ=60)
                                     ACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAG                                                                                                                <  SRR19973063.4171985/150‑1 (MQ=60)
                                     ACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAG                                                                                                                <  SRR19973063.4171785/150‑1 (MQ=60)
                                        TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973063.1000774/1‑150 (MQ=60)
                                        TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973063.1000488/1‑150 (MQ=60)
                                           GCGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAA                                                                                                          <  SRR19973063.3224929/150‑1 (MQ=60)
                                              GGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAC                                                                                                       >  SRR19973063.1621239/1‑150 (MQ=60)
                                              GGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAC                                                                                                       <  SRR19973063.3004532/150‑1 (MQ=60)
                                                 cggcatacgagatacgtctacgtgactggagttcagacgtgtgctcttccgatctAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    <  SRR19973063.2914051/95‑1 (MQ=60)
                                                 AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    <  SRR19973063.1858446/150‑1 (MQ=60)
                                                 AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    >  SRR19973063.2389575/1‑150 (MQ=60)
                                                  GCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGG                                                                                                   <  SRR19973063.3646605/150‑1 (MQ=60)
                                                    ACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGT                                                                                                 <  SRR19973063.3844728/150‑1 (MQ=60)
                                                    ACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGT                                                                                                 <  SRR19973063.3734974/150‑1 (MQ=60)
                                                    ACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGAACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACTTTGGT                                                                                                 >  SRR19973063.3513546/1‑150 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              >  SRR19973063.2627374/1‑150 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              >  SRR19973063.3321242/1‑150 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              <  SRR19973063.1351834/150‑1 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              >  SRR19973063.636297/1‑150 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              <  SRR19973063.1351806/150‑1 (MQ=60)
                                                       TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              <  SRR19973063.1669575/150‑1 (MQ=60)
                                                          TGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATC                                                                                           <  SRR19973063.5045407/150‑1 (MQ=60)
                                                           GCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCG                                                                                          >  SRR19973063.1206145/1‑150 (MQ=60)
                                                            CCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGC                                                                                         >  SRR19973063.3252905/1‑150 (MQ=60)
                                                              GCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCA                                                                                       >  SRR19973063.1188732/1‑150 (MQ=60)
                                                               CTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAC                                                                                      >  SRR19973063.3296167/1‑150 (MQ=60)
                                                               CTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAC                                                                                      <  SRR19973063.4039271/150‑1 (MQ=60)
                                                               CTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAC                                                                                      <  SRR19973063.71888/150‑1 (MQ=60)
                                                               CTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAC                                                                                      <  SRR19973063.4039329/150‑1 (MQ=60)
                                                                TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     >  SRR19973063.1047603/1‑150 (MQ=60)
                                                                TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     <  SRR19973063.2792249/150‑1 (MQ=60)
                                                                 ACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTG                                                                                    >  SRR19973063.1107277/1‑150 (MQ=60)
                                                                   GGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAA                                                                                  <  SRR19973063.3334031/150‑1 (MQ=60)
                                                                      GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                               <  SRR19973063.3172588/150‑1 (MQ=60)
                                                                      GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                               <  SRR19973063.37920/150‑1 (MQ=60)
                                                                       TTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAG                                                                              >  SRR19973063.4114697/1‑150 (MQ=60)
                                                                        TGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGG                                                                             >  SRR19973063.2636148/1‑150 (MQ=60)
                                                                            GACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGG                                                                         >  SRR19973063.4284654/1‑150 (MQ=60)
                                                                            GACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGG                                                                         <  SRR19973063.1095184/150‑1 (MQ=60)
                                                                               AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      <  SRR19973063.1077105/150‑1 (MQ=60)
                                                                                GCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACC                                                                     <  SRR19973063.2201136/150‑1 (MQ=60)
                                                                                GCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACC                                                                     <  SRR19973063.2201069/150‑1 (MQ=60)
                                                                                    CGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTCAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTA                                                                 >  SRR19973063.3029217/1‑150 (MQ=60)
                                                                                     GGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTAT                                                                <  SRR19973063.2209924/150‑1 (MQ=60)
                                                                                      GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                               <  SRR19973063.3242639/150‑1 (MQ=60)
                                                                                            GCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCG                                                         <  SRR19973063.4924308/150‑1 (MQ=60)
                                                                                            GCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCG                                                         <  SRR19973063.4922487/150‑1 (MQ=60)
                                                                                                GGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAA                                                     <  SRR19973063.1129313/150‑1 (MQ=60)
                                                                                                 GAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAC                                                    <  SRR19973063.4059645/150‑1 (MQ=60)
                                                                                                  AACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACG                                                   <  SRR19973063.1351549/150‑1 (MQ=60)
                                                                                                   ACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGG                                                  >  SRR19973063.1947066/1‑150 (MQ=60)
                                                                                                   ACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGG                                                  >  SRR19973063.438122/1‑150 (MQ=60)
                                                                                                   ACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGG                                                  <  SRR19973063.4827675/150‑1 (MQ=60)
                                                                                                      GGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGG                                               >  SRR19973063.3649262/1‑150 (MQ=60)
                                                                                                        AAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCA                                             <  SRR19973063.3496782/150‑1 (MQ=60)
                                                                                                         AAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCAT                                            <  SRR19973063.2757649/150‑1 (MQ=60)
                                                                                                          AACCGCTACAAAGGCATCCGTACGCCGCACACAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATG                                           <  SRR19973063.4531218/150‑1 (MQ=60)
                                                                                                          AACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATG                                           >  SRR19973063.2581317/1‑150 (MQ=60)
                                                                                                           ACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGA                                          >  SRR19973063.3985869/1‑150 (MQ=60)
                                                                                                            CCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAA                                         <  SRR19973063.1762742/150‑1 (MQ=60)
                                                                                                            CCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAACCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAA                                         <  SRR19973063.1226929/150‑1 (MQ=60)
                                                                                                               CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      <  SRR19973063.3576595/150‑1 (MQ=60)
                                                                                                               CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      >  SRR19973063.3166799/1‑150 (MQ=60)
                                                                                                               CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      <  SRR19973063.3045387/150‑1 (MQ=60)
                                                                                                                TACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCG                                     <  SRR19973063.4721378/150‑1 (MQ=60)
                                                                                                                TACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCG                                     <  SRR19973063.1258628/150‑1 (MQ=60)
                                                                                                                TACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCG                                     >  SRR19973063.2784140/1‑150 (MQ=60)
                                                                                                                TACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCG                                     <  SRR19973063.1258832/150‑1 (MQ=60)
                                                                                                                  CAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCG                                   >  SRR19973063.10195/1‑150 (MQ=60)
                                                                                                                  CAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCG                                   >  SRR19973063.4541159/1‑150 (MQ=60)
                                                                                                                  CAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCG                                   >  SRR19973063.1163322/1‑150 (MQ=60)
                                                                                                                   AAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGT                                  <  SRR19973063.2826063/150‑1 (MQ=60)
                                                                                                                   AAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGT                                  >  SRR19973063.3161008/1‑150 (MQ=60)
                                                                                                                      GGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAT                               <  SRR19973063.3643414/150‑1 (MQ=60)
                                                                                                                         ATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGTGTAAAGACGTGTGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCG                            <  SRR19973063.960510/150‑1 (MQ=60)
                                                                                                                           CCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGA                          >  SRR19973063.1017884/1‑150 (MQ=60)
                                                                                                                             GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                        >  SRR19973063.2888326/1‑150 (MQ=60)
                                                                                                                              TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973063.85230/150‑1 (MQ=60)
                                                                                                                               ACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTG                      <  SRR19973063.2279236/150‑1 (MQ=60)
                                                                                                                               ACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTG                      <  SRR19973063.4700765/150‑1 (MQ=60)
                                                                                                                               ACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTG                      <  SRR19973063.3714410/150‑1 (MQ=60)
                                                                                                                                 GCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCT                    >  SRR19973063.4563005/1‑150 (MQ=60)
                                                                                                                                  CCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGAagatcgg                   >  SRR19973063.454215/1‑143 (MQ=60)
                                                                                                                                   CGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGG                  <  SRR19973063.2455873/150‑1 (MQ=60)
                                                                                                                                   CGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGG                  <  SRR19973063.2455976/150‑1 (MQ=60)
                                                                                                                                   CGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGG                  >  SRR19973063.102489/1‑150 (MQ=60)
                                                                                                                                     CACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCG                <  SRR19973063.1862761/150‑1 (MQ=60)
                                                                                                                                     CACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCG                <  SRR19973063.4779841/150‑1 (MQ=60)
                                                                                                                                         AAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGG            <  SRR19973063.1905682/150‑1 (MQ=60)
                                                                                                                                          AATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGA           <  SRR19973063.2912334/150‑1 (MQ=60)
                                                                                                                                           ATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGAT          >  SRR19973063.1642993/1‑150 (MQ=60)
                                                                                                                                           ATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGAT          <  SRR19973063.689473/150‑1 (MQ=60)
                                                                                                                                              AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC       <  SRR19973063.1954867/150‑1 (MQ=60)
                                                                                                                                               AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      >  SRR19973063.3221360/1‑150 (MQ=60)
                                                                                                                                               AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      >  SRR19973063.1272413/1‑150 (MQ=60)
                                                                                                                                               AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      >  SRR19973063.4147743/1‑150 (MQ=60)
                                                                                                                                               AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      >  SRR19973063.4147682/1‑150 (MQ=60)
                                                                                                                                               AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAAGTGGGTATTATCTCCACTGAAAAAGGCTGGAACATGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      >  SRR19973063.3221316/1‑150 (MQ=60)
                                                                                                                                                GTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGA     >  SRR19973063.3127109/1‑150 (MQ=60)
                                                                                                                                                 TTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGAT    <  SRR19973063.4382354/150‑1 (MQ=60)
                                                                                                                                                 TTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGAT    >  SRR19973063.2156601/1‑150 (MQ=60)
                                                                                                                                                  TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC   <  SRR19973063.105536/150‑1 (MQ=60)
                                                                                                                                                  TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC   >  SRR19973063.327006/1‑150 (MQ=60)
                                                                                                                                                  TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC   <  SRR19973063.4652646/150‑1 (MQ=60)
                                                                                                                                                   AGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  <  SRR19973063.78376/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489250‑3489546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑