Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,489,397 C→A F676L (TTC→TTA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,489,3970CA100.0% 703.8 / NA 212F676L (TTC→TTA) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (94/118);  total (94/118)

Only 100 of 212 total aligned reads displayed.

GGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG  >  CP009273/3489251‑3489542
                                                                                                                                                  |                                                                                                                                                 
ggTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgt                                                                                                                                                >  2:3723290/1‑150 (MQ=255)
ggTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgt                                                                                                                                                >  1:1838452/1‑150 (MQ=255)
 gTCATGCCTATGCGAAAGCTCTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgtg                                                                                                                                               <  1:305856/150‑1 (MQ=255)
 gTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgtg                                                                                                                                               <  1:1619175/150‑1 (MQ=255)
    aTGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtct                                                                                                                                            <  2:26258/150‑1 (MQ=255)
    aTGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtct                                                                                                                                            >  1:644206/1‑150 (MQ=255)
     tGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtctc                                                                                                                                           >  1:2690652/1‑150 (MQ=255)
      gCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTcc                                                                                                                                          >  2:342274/1‑150 (MQ=255)
          aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                      <  2:445189/150‑1 (MQ=255)
           tGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATACGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTg                                                                                                                                     <  2:909190/150‑1 (MQ=255)
             cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                   >  1:94912/1‑150 (MQ=255)
             cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                   <  1:1762454/150‑1 (MQ=255)
             cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                   <  1:2866662/150‑1 (MQ=255)
             cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                   <  1:222714/150‑1 (MQ=255)
               aaaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAcc                                                                                                                                 >  2:2494631/1‑150 (MQ=255)
                aaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAccc                                                                                                                                >  2:2331768/1‑150 (MQ=255)
                aaGCACTGCGTATGGCGAAAACCTGCGTGGGGAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAccc                                                                                                                                <  1:242383/150‑1 (MQ=255)
                   cACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTg                                                                                                                             >  1:2714415/1‑150 (MQ=255)
                    aCTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGa                                                                                                                            >  2:1805103/1‑150 (MQ=255)
                      tGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAt                                                                                                                          <  2:3530947/150‑1 (MQ=255)
                           aTGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCa                                                                                                                     <  2:3503174/150‑1 (MQ=255)
                             ggCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGa                                                                                                                   <  1:965422/150‑1 (MQ=255)
                               cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                 >  2:2663208/1‑150 (MQ=255)
                                gAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGc                                                                                                                <  1:2569084/150‑1 (MQ=255)
                                  aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                              <  1:3495508/150‑1 (MQ=255)
                                  aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                              >  1:843763/1‑150 (MQ=255)
                                   aaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCa                                                                                                             <  2:117481/150‑1 (MQ=255)
                                    aCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAg                                                                                                            >  1:2102676/1‑150 (MQ=255)
                                       tGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGt                                                                                                         >  1:1178863/1‑150 (MQ=255)
                                               tAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGt                                                                                                 >  2:3167555/1‑150 (MQ=255)
                                               tAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGt                                                                                                 >  2:2961145/1‑150 (MQ=255)
                                                 gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                               <  2:35926/150‑1 (MQ=255)
                                                 gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                               <  2:3148602/150‑1 (MQ=255)
                                                  cACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTggg                                                                                              >  1:1033649/1‑150 (MQ=255)
                                                   aCCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGt                                                                                             <  1:2954839/150‑1 (MQ=255)
                                                    ccTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGta                                                                                            <  2:2766901/150‑1 (MQ=255)
                                                     cTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtat                                                                                           <  2:1099264/150‑1 (MQ=255)
                                                     cTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtat                                                                                           <  2:2115518/150‑1 (MQ=255)
                                                      tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatc                                                                                          <  1:2592836/150‑2 (MQ=255)
                                                       ggTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatta                                                                                         <  1:2994689/150‑1 (MQ=255)
                                                       ggTGCCGCTACGGCGTGGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatta                                                                                         <  1:3676073/150‑1 (MQ=255)
                                                          gNCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTCTTATCg                                                                                      >  1:1493322/1‑150 (MQ=255)
                                                          gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                      <  2:17662/150‑1 (MQ=255)
                                                          gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                      <  2:2037534/150‑1 (MQ=255)
                                                          gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                      <  1:3412620/150‑1 (MQ=255)
                                                          gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                      <  2:3250712/150‑1 (MQ=255)
                                                            cGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGcc                                                                                    >  2:878080/1‑150 (MQ=255)
                                                             gCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCa                                                                                   >  1:1621922/1‑150 (MQ=255)
                                                              cTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAc                                                                                  >  2:3533006/1‑150 (MQ=255)
                                                               tACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACt                                                                                 <  2:2942984/150‑1 (MQ=255)
                                                               tACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACt                                                                                 <  1:1030757/150‑1 (MQ=255)
                                                                   gCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaa                                                                             <  2:3680902/150‑1 (MQ=255)
                                                                         gCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCt                                                                       <  1:3267854/150‑1 (MQ=255)
                                                                            aCAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGa                                                                    <  1:1791548/150‑1 (MQ=255)
                                                                              aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                  <  1:406913/150‑1 (MQ=255)
                                                                              aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                  >  1:294032/1‑150 (MQ=255)
                                                                              aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                  >  2:811083/1‑150 (MQ=255)
                                                                              aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                  <  1:2593590/150‑1 (MQ=255)
                                                                               gCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAcc                                                                 <  2:3234645/150‑1 (MQ=255)
                                                                                cGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCt                                                                >  1:2402840/1‑150 (MQ=255)
                                                                                 gTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTg                                                               >  2:1667141/1‑150 (MQ=255)
                                                                                   cGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTa                                                             >  2:2416339/1‑150 (MQ=255)
                                                                                    ggCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTAt                                                            >  2:375265/1‑150 (MQ=255)
                                                                                     gCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATg                                                           <  1:3710402/150‑1 (MQ=255)
                                                                                       cTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGtt                                                         <  1:3700565/150‑1 (MQ=255)
                                                                                          ggCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGc                                                      <  1:2518090/150‑1 (MQ=255)
                                                                                          ggCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGc                                                      <  1:1942273/150‑1 (MQ=255)
                                                                                            cGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCgg                                                    >  2:2281875/1‑150 (MQ=255)
                                                                                                gAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAc                                                <  2:1039544/150‑1 (MQ=255)
                                                                                                 aaCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcg                                               >  1:133838/1‑150 (MQ=255)
                                                                                                 aaCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcg                                               <  1:2007882/150‑1 (MQ=255)
                                                                                                   cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                             <  1:112093/150‑1 (MQ=255)
                                                                                                   cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                             <  2:2503599/150‑1 (MQ=255)
                                                                                                   cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                             <  2:2503623/150‑1 (MQ=255)
                                                                                                   cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                             <  1:113667/150‑1 (MQ=255)
                                                                                                   cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                             >  2:1289108/1‑150 (MQ=255)
                                                                                                      gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                          <  2:2118711/150‑1 (MQ=255)
                                                                                                         aaCCGCTACATAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCAta                                       <  1:888444/150‑2 (MQ=255)
                                                                                                         aaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATg                                       <  1:536514/150‑1 (MQ=255)
                                                                                                           ccGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGaa                                     <  1:1215498/150‑1 (MQ=255)
                                                                                                              cTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAcc                                  >  1:1665367/1‑150 (MQ=255)
                                                                                                              cTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAcc                                  <  2:3626339/150‑1 (MQ=255)
                                                                                                                aCAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcgc                                >  2:2136317/1‑150 (MQ=255)
                                                                                                                  aaaGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGt                              >  1:3653566/1‑150 (MQ=255)
                                                                                                                  aaaGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGt                              >  1:2359041/1‑150 (MQ=255)
                                                                                                                   aaGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTc                             >  1:1239588/1‑150 (MQ=255)
                                                                                                                    aGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCa                            >  1:1281283/1‑150 (MQ=255)
                                                                                                                     ggCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTCTTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAt                           >  1:1185571/1‑150 (MQ=255)
                                                                                                                      gCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATg                          <  2:370944/150‑1 (MQ=255)
                                                                                                                          ccGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGa                      >  1:3144016/1‑150 (MQ=255)
                                                                                                                            gTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                    <  1:2198258/150‑1 (MQ=255)
                                                                                                                             tACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATct                   <  1:2280441/150‑1 (MQ=255)
                                                                                                                                 ccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgctg               <  2:1910590/150‑1 (MQ=255)
                                                                                                                                   gcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggc             <  1:3789275/150‑1 (MQ=255)
                                                                                                                                    cacaAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcg            >  2:659907/1‑150 (MQ=255)
                                                                                                                                         aaTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGa       <  1:2776022/150‑1 (MQ=255)
                                                                                                                                          aTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGat      >  2:1583234/1‑150 (MQ=255)
                                                                                                                                          aTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGat      <  2:3066697/150‑1 (MQ=255)
                                                                                                                                             aaGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatc   <  2:1838452/150‑1 (MQ=255)
                                                                                                                                              aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg  <  1:2663208/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG  >  CP009273/3489251‑3489542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489249‑3489546
                                                                                                                                                    |                                                                                                                                                     
CCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAG                                                                                                                                                      <  SRR19973061.2905904/150‑1 (MQ=60)
CCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAG                                                                                                                                                      <  SRR19973061.2904942/150‑1 (MQ=60)
  GGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGT                                                                                                                                                    >  SRR19973061.1843058/1‑150 (MQ=60)
   GTCATGCCTATGCGAAAGCTCTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTG                                                                                                                                                   <  SRR19973061.306661/150‑1 (MQ=60)
   GTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTG                                                                                                                                                   <  SRR19973061.1685200/150‑1 (MQ=60)
   GTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTG                                                                                                                                                   <  SRR19973061.1623211/150‑1 (MQ=60)
    TCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGT                                                                                                                                                  >  SRR19973061.891669/1‑150 (MQ=60)
    TCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGT                                                                                                                                                  >  SRR19973061.1276261/1‑150 (MQ=60)
      ATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCT                                                                                                                                                >  SRR19973061.645853/1‑150 (MQ=60)
       TGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTC                                                                                                                                               >  SRR19973061.2698740/1‑150 (MQ=60)
       TGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTC                                                                                                                                               >  SRR19973061.2697569/1‑150 (MQ=60)
            ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                          <  SRR19973061.3584830/150‑1 (MQ=60)
               CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       <  SRR19973061.223367/150‑1 (MQ=60)
               CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       <  SRR19973061.2874006/150‑1 (MQ=60)
               CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       >  SRR19973061.95231/1‑150 (MQ=60)
               CGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTA                                                                                                                                       <  SRR19973061.1766871/150‑1 (MQ=60)
                GAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAC                                                                                                                                      >  SRR19973061.1195637/1‑150 (MQ=60)
                  AAGCACTGCGTATGGCGAAAACCTGCGTGGGGAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCC                                                                                                                                    <  SRR19973061.243076/150‑1 (MQ=60)
                     CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                 >  SRR19973061.3225894/1‑150 (MQ=60)
                     CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                 >  SRR19973061.2950556/1‑150 (MQ=60)
                     CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                 >  SRR19973061.2721402/1‑150 (MQ=60)
                       CTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAA                                                                                                                               >  SRR19973061.3059812/1‑150 (MQ=60)
                        TGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAAT                                                                                                                              >  SRR19973061.3074617/1‑150 (MQ=60)
                           GTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGACGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGAACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTT                                                                                                                           >  SRR19973061.3688917/1‑150 (MQ=60)
                            TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          >  SRR19973061.1537344/1‑150 (MQ=60)
                            TATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTC                                                                                                                          >  SRR19973061.2863080/1‑150 (MQ=60)
                               GGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGA                                                                                                                       <  SRR19973061.2368585/150‑1 (MQ=60)
                               GGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGA                                                                                                                       <  SRR19973061.967861/150‑1 (MQ=60)
                                GCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAA                                                                                                                      <  SRR19973061.2418011/150‑1 (MQ=60)
                                  GAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGC                                                                                                                    <  SRR19973061.2575653/150‑1 (MQ=60)
                                    AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTC                                                                                                                  <  SRR19973061.3504440/150‑1 (MQ=60)
                                    AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTC                                                                                                                  >  SRR19973061.845963/1‑150 (MQ=60)
                                     AACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCA                                                                                                                 <  SRR19973061.2500984/150‑1 (MQ=60)
                                     AACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCA                                                                                                                 <  SRR19973061.1059245/150‑1 (MQ=60)
                                      ACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAG                                                                                                                >  SRR19973061.1310801/1‑150 (MQ=60)
                                      ACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAG                                                                                                                >  SRR19973061.2107957/1‑150 (MQ=60)
                                       CCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGG                                                                                                               >  SRR19973061.2629418/1‑150 (MQ=60)
                                         TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973061.1181844/1‑150 (MQ=60)
                                         TGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGT                                                                                                             >  SRR19973061.1181826/1‑150 (MQ=60)
                                             TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                         >  SRR19973061.1944969/1‑150 (MQ=60)
                                                  AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                    >  SRR19973061.228203/1‑150 (MQ=60)
                                                   GCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGG                                                                                                   >  SRR19973061.1127794/1‑150 (MQ=60)
                                                    CACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGG                                                                                                  >  SRR19973061.1036299/1‑150 (MQ=60)
                                                    CACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGG                                                                                                  >  SRR19973061.2214026/1‑150 (MQ=60)
                                                     ACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGT                                                                                                 <  SRR19973061.2963906/150‑1 (MQ=60)
                                                     ACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGT                                                                                                 <  SRR19973061.2962461/150‑1 (MQ=60)
                                                        TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACATAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATT                                                                                              <  SRR19973061.2598482/150‑1 (MQ=60)
                                                        TGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATC                                                                                              <  SRR19973061.2599486/150‑1 (MQ=60)
                                                         GGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTA                                                                                             <  SRR19973061.3002417/150‑1 (MQ=60)
                                                         GGTGCCGCTACGGCGTGGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTA                                                                                             <  SRR19973061.3685469/150‑1 (MQ=60)
                                                            GNCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTCTTATCG                                                                                          >  SRR19973061.1497066/1‑150 (MQ=60)
                                                            GCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGACCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGATCAGGGTAAAGACGTGGGTATTATCG                                                                                          >  SRR19973061.2839003/1‑150 (MQ=60)
                                                            GCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCG                                                                                          <  SRR19973061.3421382/150‑1 (MQ=60)
                                                               GCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCA                                                                                       >  SRR19973061.1625968/1‑150 (MQ=60)
                                                               GCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCA                                                                                       >  SRR19973061.1625551/1‑150 (MQ=60)
                                                                CTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCAC                                                                                      >  SRR19973061.3672477/1‑150 (MQ=60)
                                                                 TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     <  SRR19973061.96526/150‑1 (MQ=60)
                                                                 TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     <  SRR19973061.1033584/150‑1 (MQ=60)
                                                                 TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     <  SRR19973061.548306/150‑1 (MQ=60)
                                                                 TACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACT                                                                                     <  SRR19973061.1033396/150‑1 (MQ=60)
                                                                    GGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAA                                                                                  >  SRR19973061.2532961/1‑150 (MQ=60)
                                                                       GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCTGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                               <  SRR19973061.2770836/150‑1 (MQ=60)
                                                                           GCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCT                                                                           <  SRR19973061.3276271/150‑1 (MQ=60)
                                                                            CGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTG                                                                          >  SRR19973061.1772453/1‑150 (MQ=60)
                                                                              ACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGA                                                                        <  SRR19973061.1796032/150‑1 (MQ=60)
                                                                                AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      >  SRR19973061.294819/1‑150 (MQ=60)
                                                                                AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      <  SRR19973061.2599903/150‑1 (MQ=60)
                                                                                AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      <  SRR19973061.2600243/150‑1 (MQ=60)
                                                                                AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                      <  SRR19973061.408024/150‑1 (MQ=60)
                                                                                  CGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCT                                                                    >  SRR19973061.2408973/1‑150 (MQ=60)
                                                                                  CGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCT                                                                    <  SRR19973061.1798940/150‑1 (MQ=60)
                                                                                       GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                               <  SRR19973061.3716167/150‑1 (MQ=60)
                                                                                       GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                               >  SRR19973061.1652152/1‑150 (MQ=60)
                                                                                       GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                               <  SRR19973061.3719886/150‑1 (MQ=60)
                                                                                         CTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTT                                                             <  SRR19973061.3710021/150‑1 (MQ=60)
                                                                                            GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                          <  SRR19973061.1947114/150‑1 (MQ=60)
                                                                                            GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                          <  SRR19973061.2524507/150‑1 (MQ=60)
                                                                                              CGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGG                                                        >  SRR19973061.974596/1‑150 (MQ=60)
                                                                                               GTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGT                                                       <  SRR19973061.198768/150‑1 (MQ=60)
                                                                                                TGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTA                                                      <  SRR19973061.3436683/150‑1 (MQ=60)
                                                                                                   AACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACG                                                   <  SRR19973061.2012921/150‑1 (MQ=60)
                                                                                                   AACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACG                                                   >  SRR19973061.134267/1‑150 (MQ=60)
                                                                                                    ACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGG                                                  >  SRR19973061.2696920/1‑150 (MQ=60)
                                                                                                    ACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGG                                                  >  SRR19973061.2427280/1‑150 (MQ=60)
                                                                                                     CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                 <  SRR19973061.112467/150‑1 (MQ=60)
                                                                                                     CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                 <  SRR19973061.114043/150‑1 (MQ=60)
                                                                                                         AAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCA                                             <  SRR19973061.753041/150‑1 (MQ=60)
                                                                                                           AACCGCTACATAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATA                                           <  SRR19973061.890741/150‑1 (MQ=60)
                                                                                                           AACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATG                                           <  SRR19973061.537933/150‑1 (MQ=60)
                                                                                                           AACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATA                                           <  SRR19973061.39601/150‑1 (MQ=60)
                                                                                                             CCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAA                                         <  SRR19973061.1218540/150‑1 (MQ=60)
                                                                                                                CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      <  SRR19973061.589387/150‑1 (MQ=60)
                                                                                                                CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      >  SRR19973061.1669547/1‑150 (MQ=60)
                                                                                                                CTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACC                                      >  SRR19973061.2930340/1‑150 (MQ=60)
                                                                                                                    AAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGT                                  >  SRR19973061.2365037/1‑150 (MQ=60)
                                                                                                                    AAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGT                                  >  SRR19973061.3662901/1‑150 (MQ=60)
                                                                                                                     AAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC                                 >  SRR19973061.1240324/1‑150 (MQ=60)
                                                                                                                     AAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC                                 >  SRR19973061.1242667/1‑150 (MQ=60)
                                                                                                                      AGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCA                                >  SRR19973061.1284446/1‑150 (MQ=60)
                                                                                                                       GGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTCTTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAT                               >  SRR19973061.1188563/1‑150 (MQ=60)
                                                                                                                       GGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAT                               >  SRR19973061.1187060/1‑150 (MQ=60)
                                                                                                                          ATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCTGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCG                            <  SRR19973061.3295465/150‑1 (MQ=60)
                                                                                                                          ATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCG                            <  SRR19973061.2067228/150‑1 (MQ=60)
                                                                                                                            CCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGA                          >  SRR19973061.3152105/1‑150 (MQ=60)
                                                                                                                              GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                        <  SRR19973061.2203184/150‑1 (MQ=60)
                                                                                                                              GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                        <  SRR19973061.2203784/150‑1 (MQ=60)
                                                                                                                               TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAGAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973061.1386367/150‑1 (MQ=60)
                                                                                                                               TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973061.2286900/150‑1 (MQ=60)
                                                                                                                               TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                       <  SRR19973061.2286203/150‑1 (MQ=60)
                                                                                                                                     GCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGC                 <  SRR19973061.1899128/150‑1 (MQ=60)
                                                                                                                                     GCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGC                 <  SRR19973061.3798726/150‑1 (MQ=60)
                                                                                                                                     GCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGC                 <  SRR19973061.3798931/150‑1 (MQ=60)
                                                                                                                                          AAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGG            >  SRR19973061.130759/1‑150 (MQ=60)
                                                                                                                                          AAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGG            >  SRR19973061.525368/1‑150 (MQ=60)
                                                                                                                                           AATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGA           <  SRR19973061.2783163/150‑1 (MQ=60)
                                                                                                                                               AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC       <  SRR19973061.880358/150‑1 (MQ=60)
                                                                                                                                               AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC       <  SRR19973061.343183/150‑1 (MQ=60)
                                                                                                                                                AGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCG      <  SRR19973061.2670053/150‑1 (MQ=60)
                                                                                                                                                  TTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGAT    <  SRR19973061.376282/150‑1 (MQ=60)
                                                                                                                                                   TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC   <  SRR19973061.1809609/150‑1 (MQ=60)
                                                                                                                                                    AGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGACCCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  SRR19973061.3036182/1‑150 (MQ=60)
                                                                                                                                                    AGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  SRR19973061.3787771/1‑150 (MQ=60)
                                                                                                                                                    AGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  SRR19973061.3330949/1‑150 (MQ=60)
                                                                                                                                                    AGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  SRR19973061.2432493/1‑150 (MQ=60)
                                                                                                                                                    AGGTGTCTCAGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  <  SRR19973061.2337686/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATCG  >  CP009273/3489249‑3489546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑