Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,489,397 C→A 100% F676L (TTC→TTA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,489,3970CA97.7% 873.2 / 9.9 259F676L (TTC→TTA) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (110/143);  total (114/145)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 259 total aligned reads displayed.

CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  CP009273/3489250‑3489545
                                                                                                                                                   |                                                                                                                                                    
 ggTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgt                                                                                                                                                   >  1:1332149/1‑150 (MQ=255)
 ggTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgt                                                                                                                                                   >  2:1606183/1‑150 (MQ=255)
   tCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGgtgt                                                                                                                                                 >  1:2212440/1‑150 (MQ=255)
     tttacTATGCGAAAGCACTGGGTTTTGCGAAACCCTGCGTGGGTAGCACCTGGTGCCGCTTCGGCGTTGGCGACAGAGTCGGCCTCGGCGTGGTACTGGAAAACCGCTAAATAGGCTTCCGTAAGCAGCAAAAATTGAAGTTAGGTGtct                                                                                                                                               <  2:4139376/146‑1 (MQ=255)
      tGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGtctc                                                                                                                                              >  2:2034296/1‑150 (MQ=255)
          tATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGc                                                                                                                                          >  1:3987355/1‑150 (MQ=255)
           aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                         <  1:3149626/150‑1 (MQ=255)
           aTGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCt                                                                                                                                         <  2:3286332/150‑1 (MQ=255)
             gCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGt                                                                                                                                       >  2:3448221/1‑150 (MQ=255)
             gCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGt                                                                                                                                       <  2:2191008/150‑1 (MQ=255)
              cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                      <  2:1520058/150‑1 (MQ=255)
              cGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTa                                                                                                                                      <  2:1246699/150‑1 (MQ=255)
               gAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAc                                                                                                                                     <  1:1965349/150‑1 (MQ=255)
               gAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAc                                                                                                                                     >  2:2783135/1‑150 (MQ=255)
                aaaGCTCTGCTTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGTCGTGGAACTTGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAcc                                                                                                                                    <  1:875284/150‑1 (MQ=255)
                 aaGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACCGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAccc                                                                                                                                   <  1:3461641/150‑1 (MQ=255)
                     aCTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGa                                                                                                                               >  2:110040/1‑150 (MQ=255)
                         cGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGt                                                                                                                           <  1:1292124/150‑1 (MQ=255)
                            aTGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCa                                                                                                                        <  2:2485603/150‑1 (MQ=255)
                              ggCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGa                                                                                                                      >  1:409075/1‑150 (MQ=255)
                               gCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGaa                                                                                                                     <  2:3038146/150‑1 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                    <  1:972837/150‑1 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                    >  2:2881100/1‑150 (MQ=255)
                                cGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAg                                                                                                                    <  1:3129545/150‑1 (MQ=255)
                                  aaaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCt                                                                                                                  <  2:3136093/150‑1 (MQ=255)
                                  aaaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCt                                                                                                                  <  1:942080/150‑1 (MQ=255)
                                  aaaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCt                                                                                                                  >  2:3323949/1‑150 (MQ=255)
                                  aaaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCt                                                                                                                  <  1:1535358/150‑1 (MQ=255)
                                  aaaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCt                                                                                                                  >  1:2223873/1‑150 (MQ=255)
                                   aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAATGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                                 >  1:2388902/1‑150 (MQ=255)
                                   aaaCCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTc                                                                                                                 <  2:3914126/150‑1 (MQ=255)
                                        tGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGt                                                                                                            <  2:2749466/150‑1 (MQ=255)
                                        tGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGt                                                                                                            >  2:3287568/1‑150 (MQ=255)
                                          cGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTaa                                                                                                          <  1:723894/150‑1 (MQ=255)
                                            tGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAg                                                                                                        <  1:2043283/150‑1 (MQ=255)
                                              ggTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAc                                                                                                      <  2:302415/150‑1 (MQ=255)
                                                tAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGt                                                                                                    >  2:4051161/1‑150 (MQ=255)
                                                 aGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTg                                                                                                   >  2:419119/1‑150 (MQ=255)
                                                 aGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTg                                                                                                   >  2:3068752/1‑150 (MQ=255)
                                                  gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                                  <  2:1352148/150‑1 (MQ=255)
                                                  gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                                  >  1:413585/1‑150 (MQ=255)
                                                  gCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTgg                                                                                                  >  1:3520969/1‑150 (MQ=255)
                                                      cTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtat                                                                                              <  2:535580/150‑1 (MQ=255)
                                                       tGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGtatt                                                                                             <  2:1438055/150‑1 (MQ=255)
                                                          tGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATc                                                                                          >  2:1061862/1‑150 (MQ=255)
                                                          tGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGGGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATc                                                                                          <  2:2228278/150‑1 (MQ=255)
                                                           gCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCg                                                                                         <  2:499553/150‑1 (MQ=255)
                                                                 aCGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTg                                                                                   >  1:1821395/1‑150 (MQ=255)
                                                                  cGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGa                                                                                  >  2:1469451/1‑150 (MQ=255)
                                                                   ggCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaa                                                                                 >  2:3876017/1‑150 (MQ=255)
                                                                    gCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaa                                                                                <  1:1618575/150‑1 (MQ=255)
                                                                      gTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGaaaaa                                                                              <  1:2094069/150‑1 (MQ=255)
                                                                        tGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAgg                                                                            <  2:37739/150‑1 (MQ=255)
                                                                        tGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAgg                                                                            <  2:773851/150‑1 (MQ=255)
                                                                          gCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCt                                                                          <  2:685657/150‑1 (MQ=255)
                                                                           cGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTg                                                                         <  2:802359/150‑1 (MQ=255)
                                                                           cGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTg                                                                         >  2:3808321/1‑150 (MQ=255)
                                                                            gACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTgg                                                                        <  1:1978162/150‑1 (MQ=255)
                                                                             aCAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGa                                                                       <  2:1691127/150‑1 (MQ=255)
                                                                             aCAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGa                                                                       <  2:398043/150‑1 (MQ=255)
                                                                               aGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAc                                                                     <  1:373587/150‑1 (MQ=255)
                                                                                gCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAcc                                                                    <  1:1178662/150‑1 (MQ=255)
                                                                                      gCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATg                                                              >  1:1441192/1‑150 (MQ=255)
                                                                                       ccTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGt                                                             <  2:937208/150‑1 (MQ=255)
                                                                                           ggCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGc                                                         >  1:2678185/1‑150 (MQ=255)
                                                                                           ggCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGc                                                         >  1:2678176/1‑150 (MQ=255)
                                                                                            gCGTGGAACTGGAAAACCGCTACATAGTCTTCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCg                                                        <  1:3644408/150‑1 (MQ=255)
                                                                                            gCGTGGAACTGGAAAACCGCTACAAAGGCTTCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGTATGTTCTGAAGCTCTGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCg                                                        <  2:2416963/150‑1 (MQ=255)
                                                                                               tGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTa                                                     >  2:2151151/1‑150 (MQ=255)
                                                                                                 gAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTNTGTTTGCGGTAAc                                                   <  2:3736597/150‑1 (MQ=255)
                                                                                                  aaCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcg                                                  <  1:3174199/150‑1 (MQ=255)
                                                                                                    cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                                >  1:3916913/1‑150 (MQ=255)
                                                                                                    cTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAAcggc                                                <  2:3848955/150‑1 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                             >  2:46150/1‑150 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                             >  2:1272561/1‑150 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                             <  1:1047912/150‑1 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                             >  2:3943883/1‑150 (MQ=255)
                                                                                                       gAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACggcggc                                             >  2:3943839/1‑150 (MQ=255)
                                                                                                          aaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGACCCTGTATGTTTGCGGTAACGGCGGCATg                                          >  2:3349755/1‑150 (MQ=255)
                                                                                                          aaCCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATg                                          <  1:2017097/150‑1 (MQ=255)
                                                                                                             cGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCCGAAGATCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGGATGTTTGCGGTAACGGCGGCATGaaa                                       >  2:1958085/1‑150 (MQ=255)
                                                                                                              gCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAc                                      >  1:3956218/1‑150 (MQ=255)
                                                                                                              gCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAc                                      >  1:4065608/1‑150 (MQ=255)
                                                                                                                tACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACcg                                    <  2:2866515/150‑1 (MQ=255)
                                                                                                                      ggCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAt                              <  1:2993932/150‑1 (MQ=255)
                                                                                                                        cATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGc                            <  1:2538508/150‑1 (MQ=255)
                                                                                                                             gTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATc                       >  1:433498/1‑150 (MQ=255)
                                                                                                                              tACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATct                      <  1:3282239/150‑1 (MQ=255)
                                                                                                                                  ccgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATctgctg                  <  2:2266666/150‑1 (MQ=255)
                                                                                                                                   cgcACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTgg                 <  2:364335/150‑1 (MQ=255)
                                                                                                                                     cacaAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcg               <  1:2555704/150‑1 (MQ=255)
                                                                                                                                       caAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTggcggc             >  2:1829341/1‑150 (MQ=255)
                                                                                                                                         aaaTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGcggcgg           >  1:379344/1‑150 (MQ=255)
                                                                                                                                           aTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGat         <  2:558768/150‑1 (MQ=255)
                                                                                                                                            tGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGata        <  2:3904855/150‑1 (MQ=255)
                                                                                                                                            tGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGata        <  1:2773921/150‑1 (MQ=255)
                                                                                                                                             gAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGatat       <  1:2828109/150‑1 (MQ=255)
                                                                                                                                             gAAGGTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGatat       <  1:2828020/150‑1 (MQ=255)
                                                                                                                                               aGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcg     >  2:2502224/1‑150 (MQ=255)
                                                                                                                                                  tCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATatcgatc  <  1:1677179/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  CP009273/3489250‑3489545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  CP009273/3489248‑3489545
                                                                                                                                                     |                                                                                                                                                    
ACCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTA                                                                                                                                                      >  SRR19973074.430523/1‑150 (MQ=60)
 CCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAG                                                                                                                                                     <  SRR19973074.81068/150‑1 (MQ=60)
  CGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGG                                                                                                                                                    <  SRR19973074.497268/150‑1 (MQ=60)
   GGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGT                                                                                                                                                   >  SRR19973074.1334640/1‑150 (MQ=60)
     TCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGT                                                                                                                                                 >  SRR19973074.2216603/1‑150 (MQ=60)
       ATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCT                                                                                                                                               <  SRR19973074.960630/150‑1 (MQ=60)
        TGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTC                                                                                                                                              <  SRR19973074.2291655/150‑1 (MQ=60)
          CCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGTGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCG                                                                                                                                            >  SRR19973074.289904/1‑150 (MQ=60)
            TATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGC                                                                                                                                          >  SRR19973074.3994714/1‑150 (MQ=60)
            TATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGC                                                                                                                                          >  SRR19973074.3994761/1‑150 (MQ=60)
             ATGCGAAATCACTGCGTATGGCGAAAACCTGCGTGGGGAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCAGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                         <  SRR19973074.3155420/150‑1 (MQ=60)
             ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                         <  SRR19973074.3878822/150‑1 (MQ=60)
             ATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCT                                                                                                                                         <  SRR19973074.3155409/150‑1 (MQ=60)
                 GAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTAC                                                                                                                                     <  SRR19973074.1969100/150‑1 (MQ=60)
                  AAAGCTCTGCTTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGTCGTGGAACTTGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACC                                                                                                                                    <  SRR19973074.876968/150‑1 (MQ=60)
                   AAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACCGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCC                                                                                                                                   <  SRR19973074.3467699/150‑1 (MQ=60)
                   AAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACCGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCC                                                                                                                                   <  SRR19973074.3468000/150‑1 (MQ=60)
                    AGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCG                                                                                                                                  <  SRR19973074.2649276/150‑1 (MQ=60)
                     GCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGT                                                                                                                                 <  SRR19973074.2925592/150‑1 (MQ=60)
                      CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                >  SRR19973074.141975/1‑150 (MQ=60)
                      CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTG                                                                                                                                >  SRR19973074.2099584/1‑150 (MQ=60)
                      CACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGGGGACCTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTAAGGTGTCTCCGGCTGTAACCGTG                                                                                                                                >  SRR19973074.2364124/1‑150 (MQ=60)
                           CGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGT                                                                                                                           >  SRR19973074.2412519/1‑150 (MQ=60)
                           CGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGT                                                                                                                           <  SRR19973074.1294575/150‑1 (MQ=60)
                                GGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGA                                                                                                                      >  SRR19973074.409859/1‑150 (MQ=60)
                                GGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGA                                                                                                                      >  SRR19973074.1312159/1‑150 (MQ=60)
                                  CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                    <  SRR19973074.3135274/150‑1 (MQ=60)
                                  CGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAG                                                                                                                    <  SRR19973074.974668/150‑1 (MQ=60)
                                    AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                  <  SRR19973074.1538269/150‑1 (MQ=60)
                                    AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                  <  SRR19973074.943875/150‑1 (MQ=60)
                                    AAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCT                                                                                                                  >  SRR19973074.2228061/1‑150 (MQ=60)
                                     AAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAATGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTC                                                                                                                 >  SRR19973074.2393330/1‑150 (MQ=60)
                                        CCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGG                                                                                                              <  SRR19973074.3854198/150‑1 (MQ=60)
                                           GCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTA                                                                                                           <  SRR19973074.3136812/150‑1 (MQ=60)
                                            CGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAA                                                                                                          <  SRR19973074.725252/150‑1 (MQ=60)
                                            CGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAA                                                                                                          >  SRR19973074.1827691/1‑150 (MQ=60)
                                             GTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAA                                                                                                         >  SRR19973074.1640208/1‑150 (MQ=60)
                                              TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                        <  SRR19973074.2047131/150‑1 (MQ=60)
                                              TGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAG                                                                                                        <  SRR19973074.2047473/150‑1 (MQ=60)
                                                GGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGAC                                                                                                      <  SRR19973074.2171256/150‑1 (MQ=60)
                                                 GTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACG                                                                                                     >  SRR19973074.3149919/1‑150 (MQ=60)
                                                  TAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGT                                                                                                    >  SRR19973074.4086928/1‑150 (MQ=60)
                                                   AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                   >  SRR19973074.1567723/1‑150 (MQ=60)
                                                   AGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTG                                                                                                   >  SRR19973074.1567161/1‑150 (MQ=60)
                                                    GCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGG                                                                                                  >  SRR19973074.3527448/1‑150 (MQ=60)
                                                    GCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGG                                                                                                  >  SRR19973074.414376/1‑150 (MQ=60)
                                                     CACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGG                                                                                                 >  SRR19973074.3987916/1‑150 (MQ=60)
                                                     CACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGG                                                                                                 >  SRR19973074.3987895/1‑150 (MQ=60)
                                                       CCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTA                                                                                               <  SRR19973074.2697918/150‑1 (MQ=60)
                                                            TGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATC                                                                                          <  SRR19973074.3680785/150‑1 (MQ=60)
                                                                   ACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTG                                                                                   >  SRR19973074.1824867/1‑150 (MQ=60)
                                                                     GGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAA                                                                                 <  SRR19973074.2017318/150‑1 (MQ=60)
                                                                      GCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAA                                                                                <  SRR19973074.1621648/150‑1 (MQ=60)
                                                                        GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                              >  SRR19973074.4085983/1‑150 (MQ=60)
                                                                        GTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAA                                                                              <  SRR19973074.2097988/150‑1 (MQ=60)
                                                                          TGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGG                                                                            <  SRR19973074.2661374/150‑1 (MQ=60)
                                                                            GCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCT                                                                          <  SRR19973074.1114029/150‑1 (MQ=60)
                                                                              GACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGG                                                                        <  SRR19973074.1526139/150‑1 (MQ=60)
                                                                              GACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGG                                                                        <  SRR19973074.1981821/150‑1 (MQ=60)
                                                                              GACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGG                                                                        <  SRR19973074.1981927/150‑1 (MQ=60)
                                                                                 AGCGTCGGCCTCGGCGTGGAACTGGAAACCCGCTACAACGGCATCCATACGCCGCACACACTGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                     <  SRR19973074.1240019/150‑1 (MQ=60)
                                                                                 AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                     >  SRR19973074.417211/1‑150 (MQ=60)
                                                                                 AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                     <  SRR19973074.374286/150‑1 (MQ=60)
                                                                                 AGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAAC                                                                     <  SRR19973074.374287/150‑1 (MQ=60)
                                                                                  GCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACC                                                                    <  SRR19973074.1180907/150‑1 (MQ=60)
                                                                                    GTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGTCTGGAAACTG                                                                  >  SRR19973074.362024/1‑150 (MQ=60)
                                                                                    GTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTG                                                                  >  SRR19973074.362036/1‑150 (MQ=60)
                                                                                        GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                              >  SRR19973074.1443765/1‑150 (MQ=60)
                                                                                        GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                              >  SRR19973074.1443883/1‑150 (MQ=60)
                                                                                        GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                              <  SRR19973074.3390021/150‑1 (MQ=60)
                                                                                        GCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATG                                                              >  SRR19973074.1983227/1‑150 (MQ=60)
                                                                                            CGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTG                                                          >  SRR19973074.3789516/1‑150 (MQ=60)
                                                                                            CGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTG                                                          >  SRR19973074.202494/1‑150 (MQ=60)
                                                                                             GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                         >  SRR19973074.2683158/1‑150 (MQ=60)
                                                                                             GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                         >  SRR19973074.2683166/1‑150 (MQ=60)
                                                                                             GGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGC                                                         >  SRR19973074.2683167/1‑150 (MQ=60)
                                                                                              GCGTGGAACTGGAAAACCGCTACATAGTCTTCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCG                                                        <  SRR19973074.3651146/150‑1 (MQ=60)
                                                                                              GCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCG                                                        <  SRR19973074.222101/150‑1 (MQ=60)
                                                                                                 TGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTA                                                     <  SRR19973074.308160/150‑1 (MQ=60)
                                                                                                    AACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACG                                                  <  SRR19973074.3180041/150‑1 (MQ=60)
                                                                                                      CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                >  SRR19973074.3924117/1‑150 (MQ=60)
                                                                                                      CTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGC                                                >  SRR19973074.3924178/1‑150 (MQ=60)
                                                                                                       TGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCG                                               >  SRR19973074.1286963/1‑150 (MQ=60)
                                                                                                       TGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCG                                               <  SRR19973074.1665455/150‑1 (MQ=60)
                                                                                                       TGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCG                                               >  SRR19973074.933127/1‑150 (MQ=60)
                                                                                                         GAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGC                                             <  SRR19973074.1049839/150‑1 (MQ=60)
                                                                                                            AACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATG                                          <  SRR19973074.2020918/150‑1 (MQ=60)
                                                                                                                GCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAC                                      >  SRR19973074.3197709/1‑150 (MQ=60)
                                                                                                                GCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAC                                      >  SRR19973074.1715542/1‑150 (MQ=60)
                                                                                                                GCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAC                                      >  SRR19973074.3963510/1‑150 (MQ=60)
                                                                                                                GCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAC                                      >  SRR19973074.4073127/1‑150 (MQ=60)
                                                                                                                   ACAAAGGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGC                                   >  SRR19973074.82916/1‑150 (MQ=60)
                                                                                                                        GGCATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCAT                              <  SRR19973074.2999494/150‑1 (MQ=60)
                                                                                                                          CATCCGTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGC                            <  SRR19973074.2543237/150‑1 (MQ=60)
                                                                                                                               GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                       >  SRR19973074.65200/1‑150 (MQ=60)
                                                                                                                               GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                       >  SRR19973074.4141979/1‑150 (MQ=60)
                                                                                                                               GTACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATC                       >  SRR19973074.434334/1‑150 (MQ=60)
                                                                                                                                TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                      <  SRR19973074.4142344/150‑1 (MQ=60)
                                                                                                                                TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                      <  SRR19973074.3288332/150‑1 (MQ=60)
                                                                                                                                TACGCCGCACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCT                      <  SRR19973074.2038135/150‑1 (MQ=60)
                                                                                                                                       CACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCG               <  SRR19973074.2560474/150‑1 (MQ=60)
                                                                                                                                        ACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGG              >  SRR19973074.3469671/1‑150 (MQ=60)
                                                                                                                                        ACAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGG              >  SRR19973074.2559629/1‑150 (MQ=60)
                                                                                                                                         CAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGC             <  SRR19973074.1850708/150‑1 (MQ=60)
                                                                                                                                         CAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGC             >  SRR19973074.2696767/1‑150 (MQ=60)
                                                                                                                                         CAAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGC             <  SRR19973074.907551/150‑1 (MQ=60)
                                                                                                                                          AAAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCG            >  SRR19973074.2786793/1‑150 (MQ=60)
                                                                                                                                           AAATGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGG           >  SRR19973074.380058/1‑150 (MQ=60)
                                                                                                                                            AATGAATTTAGTTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGA          <  SRR19973074.2442101/150‑1 (MQ=60)
                                                                                                                                              TGAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATA        <  SRR19973074.2779012/150‑1 (MQ=60)
                                                                                                                                               GAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATAT       <  SRR19973074.2833342/150‑1 (MQ=60)
                                                                                                                                               GAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATAT       <  SRR19973074.2833274/150‑1 (MQ=60)
                                                                                                                                               GAAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATAT       <  SRR19973074.2833261/150‑1 (MQ=60)
                                                                                                                                               GAAGGTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATAT       <  SRR19973074.2833253/150‑1 (MQ=60)
                                                                                                                                                AAGTTAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATC      >  SRR19973074.3221352/1‑150 (MQ=60)
                                                                                                                                                    TCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  <  SRR19973074.1680326/150‑1 (MQ=60)
                                                                                                                                                    TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  SRR19973074.1329679/1‑150 (MQ=60)
                                                                                                                                                    TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  SRR19973074.1329687/1‑150 (MQ=60)
                                                                                                                                                    TAGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  <  SRR19973074.3827787/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ACCGGTCATGCCTATGCGAAAGCACTGCGTATGGCGAAAACCTGCGTGGGTAGCACCTGGTGCCGCTACGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTCATGCGGATCTGCTGGCGGCGGATATCGATC  >  CP009273/3489248‑3489545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑