Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,634,417 T→G K249N (AAA→AAC)  ispG ← 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,634,4170TG100.0% 956.8 / NA 241K249N (AAA→AAC) ispG1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (133/108);  total (133/108)

Only 100 of 246 total aligned reads displayed.

CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTG  >  CP009273/2634270‑2634563
                                                                                                                                                   |                                                                                                                                                  
   cGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGAt                                                                                                                                               <  2:1189363/150‑1 (MQ=255)
     gAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGAtct                                                                                                                                             <  2:3517579/150‑1 (MQ=255)
     gAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGAtct                                                                                                                                             <  1:299455/150‑1 (MQ=255)
      aGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGAtctc                                                                                                                                            >  2:811303/1‑150 (MQ=255)
      aGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGAtctc                                                                                                                                            >  1:3451303/1‑150 (MQ=255)
       gTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATctct                                                                                                                                           <  1:2675322/150‑1 (MQ=255)
       gTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATctct                                                                                                                                           <  1:3745132/150‑1 (MQ=255)
         gatgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTc                                                                                                                                         >  2:1239011/1‑150 (MQ=255)
            gatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGAc                                                                                                                                      >  2:3244685/1‑150 (MQ=255)
            gatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGAc                                                                                                                                      <  1:1493557/150‑1 (MQ=255)
             atatCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGAcc                                                                                                                                     >  2:550060/1‑150 (MQ=255)
               atCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCgg                                                                                                                                   >  2:357485/1‑150 (MQ=255)
                   tCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCg                                                                                                                               <  2:2279899/150‑1 (MQ=255)
                   tCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCg                                                                                                                               >  1:809469/1‑150 (MQ=255)
                      aGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGgcc                                                                                                                            <  1:1846110/150‑1 (MQ=255)
                           ttGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAg                                                                                                                       <  1:683850/150‑1 (MQ=255)
                            tGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGc                                                                                                                      >  2:678218/1‑150 (MQ=255)
                            tGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGc                                                                                                                      >  2:771587/1‑150 (MQ=255)
                             gCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCg                                                                                                                     >  1:2207851/1‑150 (MQ=255)
                             gCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCg                                                                                                                     >  2:2220341/1‑150 (MQ=255)
                               tCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGAt                                                                                                                   <  1:1985590/150‑1 (MQ=255)
                                ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATa                                                                                                                  <  2:3027507/150‑1 (MQ=255)
                                 cAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAc                                                                                                                 >  1:3375609/1‑150 (MQ=255)
                                  aGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAcg                                                                                                                >  1:2344402/1‑150 (MQ=255)
                                    cgcgTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAcgcg                                                                                                              <  2:601149/150‑1 (MQ=255)
                                     gcgTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACgcgc                                                                                                             >  1:2572069/1‑150 (MQ=255)
                                         tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCg                                                                                                         >  2:1952032/1‑150 (MQ=255)
                                         tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCg                                                                                                         >  2:40671/1‑150 (MQ=255)
                                           aCCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgtg                                                                                                       <  1:1992191/150‑1 (MQ=255)
                                             cGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTc                                                                                                     <  1:3222322/150‑1 (MQ=255)
                                             cGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTc                                                                                                     <  2:2776342/150‑1 (MQ=255)
                                                aCCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGcc                                                                                                  >  1:207442/1‑150 (MQ=255)
                                                aCCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGcc                                                                                                  <  2:1072435/150‑1 (MQ=255)
                                                     tAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGc                                                                                             >  1:3075034/1‑150 (MQ=255)
                                                       aCATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCt                                                                                           >  1:2786279/1‑150 (MQ=255)
                                                       aCATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCt                                                                                           >  1:1978321/1‑150 (MQ=255)
                                                       aCATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCt                                                                                           >  1:1878397/1‑150 (MQ=255)
                                                         aTCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTc                                                                                         <  2:2762939/150‑1 (MQ=255)
                                                           cAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAg                                                                                       <  2:3446554/150‑1 (MQ=255)
                                                           cAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAg                                                                                       >  1:961012/1‑150 (MQ=255)
                                                              aTTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAca                                                                                    >  2:1387241/1‑150 (MQ=255)
                                                              aTTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAca                                                                                    <  2:2975980/150‑1 (MQ=255)
                                                               ttCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGACACGCGCAGCGTGTCGCCGATGCCTTCAGAcag                                                                                   <  1:3462449/150‑1 (MQ=255)
                                                                 ccTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagca                                                                                 <  2:1962750/150‑1 (MQ=255)
                                                                    gACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagcagca                                                                              >  1:284814/1‑150 (MQ=255)
                                                                    gACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagcagca                                                                              >  1:2380795/1‑150 (MQ=255)
                                                                     aCGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagcagcag                                                                             <  2:2385818/150‑1 (MQ=255)
                                                                         gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                         <  1:1181543/150‑1 (MQ=255)
                                                                         gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                         <  2:2494430/150‑1 (MQ=255)
                                                                         gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                         >  1:733071/1‑150 (MQ=255)
                                                                         gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                         >  2:3625115/1‑150 (MQ=255)
                                                                         gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                         >  1:2946131/1‑150 (MQ=255)
                                                                          aaCAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTa                                                                        <  2:2854108/150‑1 (MQ=255)
                                                                             aGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAc                                                                     <  2:1937109/150‑1 (MQ=255)
                                                                              ggTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAcc                                                                    >  1:1568856/1‑150 (MQ=255)
                                                                                 cGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAAt                                                                 >  2:1286846/1‑150 (MQ=255)
                                                                                    gCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGc                                                              <  1:3586860/150‑1 (MQ=255)
                                                                                       ggCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGa                                                           <  1:1545022/150‑1 (MQ=255)
                                                                                        gCGATGAAGTTGATCCCTCGCGAACGGTTACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAt                                                          <  1:2517328/150‑1 (MQ=255)
                                                                                         cGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAtt                                                         <  2:3438933/150‑1 (MQ=255)
                                                                                         cGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAtt                                                         >  2:1504914/1‑150 (MQ=255)
                                                                                          gatgatGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAttt                                                        <  2:806314/150‑1 (MQ=255)
                                                                                          gATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAttt                                                        <  2:3343544/150‑1 (MQ=255)
                                                                                           aTGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAtttt                                                       <  2:3240519/150‑1 (MQ=255)
                                                                                             gAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTAc                                                     <  2:1671987/150‑1 (MQ=255)
                                                                                             gAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTAc                                                     >  2:2569197/1‑150 (MQ=255)
                                                                                                gTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGc                                                  >  2:1683960/1‑150 (MQ=255)
                                                                                                 ttGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGcc                                                 <  1:3162491/150‑1 (MQ=255)
                                                                                                  tGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCACTGGCGGATTTTACTGccc                                                >  2:2884066/1‑150 (MQ=255)
                                                                                                      cccTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCt                                            <  1:3535110/150‑1 (MQ=255)
                                                                                                           gcgAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTgcgcg                                       <  2:1362546/150‑1 (MQ=255)
                                                                                                             gAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGca                                     >  2:290166/1‑150 (MQ=255)
                                                                                                               aCGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcacc                                   >  1:3320420/1‑150 (MQ=255)
                                                                                                                  gATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTgcg                                         <  1:2463949/141‑1 (MQ=255)
                                                                                                                  gATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTgcg                                         >  2:2463949/1‑141 (MQ=255)
                                                                                                                   aTACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCg                               >  1:807861/1‑150 (MQ=255)
                                                                                                                   aTACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCg                               >  2:334570/1‑150 (MQ=255)
                                                                                                                     aCGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGc                             >  1:432950/1‑150 (MQ=255)
                                                                                                                      cGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCt                            <  1:1239011/150‑1 (MQ=255)
                                                                                                                       gaaGCGATATCAAAATATCGAAACCGACGTTGATCTCTACGACCGGAACGGCCCCCAGCGAAACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCACTGTCGGATTTTACTGCCCCGCTGCGCGCACCACCgccaa                           >  2:1139048/3‑146 (MQ=255)
                                                                                                                       gCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCtt                           >  1:747621/1‑150 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTc                          <  2:987170/150‑1 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTc                          <  2:1206199/150‑1 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTc                          <  1:159765/150‑1 (MQ=255)
                                                                                                                         aGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCg                         >  2:952239/1‑150 (MQ=255)
                                                                                                                           cGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGt                       >  2:961337/1‑150 (MQ=255)
                                                                                                                           cGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGt                       <  1:2170705/150‑1 (MQ=255)
                                                                                                                            gATTTCAAAATATCGAAACCGACGGTGATCTCGTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGNg                      <  1:1295145/150‑1 (MQ=255)
                                                                                                                             aTTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGa                     >  1:665515/1‑150 (MQ=255)
                                                                                                                              tttCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAt                    <  1:3244685/150‑1 (MQ=255)
                                                                                                                              tttCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAt                    <  2:3153492/150‑1 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATc                   <  1:1314541/150‑1 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATc                   >  2:2294174/1‑150 (MQ=255)
                                                                                                                                     atatCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGa             >  2:1552076/1‑150 (MQ=255)
                                                                                                                                      tatCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCACTGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGAt            <  2:2726860/150‑1 (MQ=255)
                                                                                                                                       atCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATg           >  1:2844725/1‑150 (MQ=255)
                                                                                                                                            aaCCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACg      >  1:2277030/1‑150 (MQ=255)
                                                                                                                                             aCCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACgg     >  1:100477/1‑150 (MQ=255)
                                                                                                                                             aCCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACgg     >  2:214266/1‑150 (MQ=255)
                                                                                                                                               cGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCt   <  1:678218/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTG  >  CP009273/2634270‑2634563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGATC  >  CP009273/2634269‑2634566
                                                                                                                                                    |                                                                                                                                                     
CCATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGAGAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGT                                                                                                                                                      <  SRR19973061.3294243/150‑1 (MQ=60)
 CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCTATTTCAAAATATCGAAACCGACGTT                                                                                                                                                     <  SRR19973061.1927096/150‑1 (MQ=60)
 CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTT                                                                                                                                                     <  SRR19973061.2436906/150‑1 (MQ=60)
 CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTT                                                                                                                                                     <  SRR19973061.429648/150‑1 (MQ=60)
 CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGCCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTT                                                                                                                                                     >  SRR19973061.734382/1‑150 (MQ=60)
      GAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCT                                                                                                                                                <  SRR19973061.300252/150‑1 (MQ=60)
       AGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTC                                                                                                                                               >  SRR19973061.3460161/1‑150 (MQ=60)
        GTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCT                                                                                                                                              <  SRR19973061.2682205/150‑1 (MQ=60)
        GTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCT                                                                                                                                              <  SRR19973061.2377065/150‑1 (MQ=60)
        GTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCT                                                                                                                                              <  SRR19973061.3754716/150‑1 (MQ=60)
             GATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGAC                                                                                                                                         <  SRR19973061.1497301/150‑1 (MQ=60)
               TATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCG                                                                                                                                       >  SRR19973061.2205146/1‑150 (MQ=60)
                   TTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATC                                                                                                                                   >  SRR19973061.684501/1‑150 (MQ=60)
                    TCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCG                                                                                                                                  <  SRR19973061.1302454/150‑1 (MQ=60)
                    TCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCG                                                                                                                                  >  SRR19973061.811585/1‑150 (MQ=60)
                       AGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCC                                                                                                                               <  SRR19973061.2213712/150‑1 (MQ=60)
                       AGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCC                                                                                                                               <  SRR19973061.1850728/150‑1 (MQ=60)
                          CGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCC                                                                                                                            <  SRR19973061.1187380/150‑1 (MQ=60)
                           GTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCA                                                                                                                           >  SRR19973061.3747140/1‑150 (MQ=60)
                            TTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAG                                                                                                                          <  SRR19973061.685623/150‑1 (MQ=60)
                              GCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCG                                                                                                                        >  SRR19973061.2213324/1‑150 (MQ=60)
                              GCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCG                                                                                                                        >  SRR19973061.2213397/1‑150 (MQ=60)
                                TCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGAT                                                                                                                      <  SRR19973061.1990550/150‑1 (MQ=60)
                                TCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGAT                                                                                                                      <  SRR19973061.1990561/150‑1 (MQ=60)
                                  CAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAC                                                                                                                    >  SRR19973061.3384284/1‑150 (MQ=60)
                                   AGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACG                                                                                                                   >  SRR19973061.2350357/1‑150 (MQ=60)
                                      GCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGC                                                                                                                >  SRR19973061.2578501/1‑150 (MQ=60)
                                      GCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGC                                                                                                                >  SRR19973061.3224831/1‑150 (MQ=60)
                                      GCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGC                                                                                                                >  SRR19973061.2578650/1‑150 (MQ=60)
                                        GTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGACGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGACCGCCAGCGATACGCGCAG                                                                                                              >  SRR19973061.3221599/1‑150 (MQ=60)
                                        GTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAG                                                                                                              >  SRR19973061.800516/1‑150 (MQ=60)
                                          TAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCG                                                                                                            <  SRR19973061.1412236/150‑1 (MQ=60)
                                          TAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCG                                                                                                            <  SRR19973061.3314237/150‑1 (MQ=60)
                                            ACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTG                                                                                                          <  SRR19973061.1997111/150‑1 (MQ=60)
                                            ACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTG                                                                                                          <  SRR19973061.1997180/150‑1 (MQ=60)
                                              CGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTC                                                                                                        <  SRR19973061.3231006/150‑1 (MQ=60)
                                              CGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTC                                                                                                        <  SRR19973061.3230605/150‑1 (MQ=60)
                                               GTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCG                                                                                                       >  SRR19973061.3778491/1‑150 (MQ=60)
                                                TACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGC                                                                                                      >  SRR19973061.976038/1‑150 (MQ=60)
                                                TACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGC                                                                                                      >  SRR19973061.2733872/1‑150 (MQ=60)
                                                TACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGC                                                                                                      >  SRR19973061.2733964/1‑150 (MQ=60)
                                                 ACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCC                                                                                                     >  SRR19973061.208064/1‑150 (MQ=60)
                                                 ACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCC                                                                                                     >  SRR19973061.307991/1‑150 (MQ=60)
                                                 ACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCC                                                                                                     >  SRR19973061.1290052/1‑150 (MQ=60)
                                                      TAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGC                                                                                                >  SRR19973061.3082969/1‑150 (MQ=60)
                                                       AACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCC                                                                                               <  SRR19973061.2084085/150‑1 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              >  SRR19973061.2793444/1‑150 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              >  SRR19973061.67147/1‑150 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              >  SRR19973061.1883073/1‑150 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              <  SRR19973061.2391683/150‑1 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              >  SRR19973061.1883069/1‑150 (MQ=60)
                                                        ACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCT                                                                                              >  SRR19973061.1983268/1‑150 (MQ=60)
                                                            CAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAG                                                                                          >  SRR19973061.963437/1‑150 (MQ=60)
                                                                TTCCTGACGCGAACTGGTCGGGCTGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCTAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGACACGCGCAGCGTGTCGCCGATGCCTTCAGACAG                                                                                      <  SRR19973061.1570713/150‑1 (MQ=60)
                                                                TTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCTATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGACACGCGCAGCGTGTCGCCGATGCCTTCAGACAG                                                                                      <  SRR19973061.3471295/150‑1 (MQ=60)
                                                                TTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAG                                                                                      <  SRR19973061.3471417/150‑1 (MQ=60)
                                                                TTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGACACGCGCAGCGTGTCGCCGATGCCTTCAGACAG                                                                                      <  SRR19973061.3471323/150‑1 (MQ=60)
                                                                  CCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCA                                                                                    <  SRR19973061.306534/150‑1 (MQ=60)
                                                                    TNACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGC                                                                                  >  SRR19973061.1859261/1‑150 (MQ=60)
                                                                    TGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGC                                                                                  >  SRR19973061.2807496/1‑150 (MQ=60)
                                                                     GACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCA                                                                                 >  SRR19973061.2386858/1‑150 (MQ=60)
                                                                     GACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCA                                                                                 >  SRR19973061.285578/1‑150 (MQ=60)
                                                                     GACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCA                                                                                 >  SRR19973061.3478432/1‑150 (MQ=60)
                                                                         CGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACC                                                                             <  SRR19973061.1593434/150‑1 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            >  SRR19973061.734805/1‑150 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            >  SRR19973061.734993/1‑150 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            >  SRR19973061.2953788/1‑150 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            >  SRR19973061.2953716/1‑150 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            >  SRR19973061.2953478/1‑150 (MQ=60)
                                                                          GAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCT                                                                            <  SRR19973061.1184527/150‑1 (MQ=60)
                                                                             CAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAA                                                                         <  SRR19973061.2568876/150‑1 (MQ=60)
                                                                               GGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACC                                                                       >  SRR19973061.1572755/1‑150 (MQ=60)
                                                                                   GGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATG                                                                   <  SRR19973061.507380/150‑1 (MQ=60)
                                                                                     GCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGC                                                                 <  SRR19973061.3596053/150‑1 (MQ=60)
                                                                                        GGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGA                                                              <  SRR19973061.2063493/150‑1 (MQ=60)
                                                                                        GGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGA                                                              <  SRR19973061.1548789/150‑1 (MQ=60)
                                                                                        GGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGA                                                              <  SRR19973061.1548868/150‑1 (MQ=60)
                                                                                         GCGATGAAGTTGATCCCTCGCGAACGGTTACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAT                                                             <  SRR19973061.2523744/150‑1 (MQ=60)
                                                                                          CGATGAAGTTGATCCCTCGCGATCGGATACGAAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATT                                                            <  SRR19973061.882542/150‑1 (MQ=60)
                                                                                            ATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTT                                                          >  SRR19973061.1410385/1‑150 (MQ=60)
                                                                                            ATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTT                                                          >  SRR19973061.3312720/1‑150 (MQ=60)
                                                                                              GAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTAC                                                        >  SRR19973061.1550708/1‑150 (MQ=60)
                                                                                                  TTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCC                                                    <  SRR19973061.3170613/150‑1 (MQ=60)
                                                                                                       CCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCT                                               <  SRR19973061.3544171/150‑1 (MQ=60)
                                                                                                       CCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCT                                               <  SRR19973061.3544166/150‑1 (MQ=60)
                                                                                                       CCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCT                                               >  SRR19973061.2029176/1‑150 (MQ=60)
                                                                                                          ttccgatctGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCGTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCG                                            <  SRR19973061.2470223/141‑1 (MQ=60)
                                                                                                             CGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGC                                         >  SRR19973061.3417954/1‑150 (MQ=60)
                                                                                                               AACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCAC                                       >  SRR19973061.3342228/1‑150 (MQ=60)
                                                                                                               AACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCAC                                       >  SRR19973061.3417803/1‑150 (MQ=60)
                                                                                                               AACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCAC                                       >  SRR19973061.825844/1‑150 (MQ=60)
                                                                                                                ACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACC                                      >  SRR19973061.3328982/1‑150 (MQ=60)
                                                                                                                    ATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCG                                  >  SRR19973061.809979/1‑150 (MQ=60)
                                                                                                                    ATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCG                                  >  SRR19973061.809975/1‑150 (MQ=60)
                                                                                                                      ACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGTCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGC                                >  SRR19973061.2346127/1‑150 (MQ=60)
                                                                                                                      ACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGC                                >  SRR19973061.434131/1‑150 (MQ=60)
                                                                                                                       CGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCT                               <  SRR19973061.1242089/150‑1 (MQ=60)
                                                                                                                        GCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTT                              >  SRR19973061.749416/1‑150 (MQ=60)
                                                                                                                        GCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTT                              >  SRR19973061.749584/1‑150 (MQ=60)
                                                                                                                         CAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTC                             <  SRR19973061.160265/150‑1 (MQ=60)
                                                                                                                         CAGCGATTTCAAAATATCGAAACCGACGTTGATCTCGTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTC                             <  SRR19973061.2518288/150‑1 (MQ=60)
                                                                                                                            CGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGT                          <  SRR19973061.2176157/150‑1 (MQ=60)
                                                                                                                             GATTTCAAAATATCGAAACCGACGGTGATCTCGTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGNG                         <  SRR19973061.1298367/150‑1 (MQ=60)
                                                                                                                              ATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGA                        >  SRR19973061.1256806/1‑150 (MQ=60)
                                                                                                                              ATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGA                        >  SRR19973061.667226/1‑150 (MQ=60)
                                                                                                                               TTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAT                       <  SRR19973061.1091272/150‑1 (MQ=60)
                                                                                                                               TTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAT                       <  SRR19973061.3253033/150‑1 (MQ=60)
                                                                                                                               TTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAT                       >  SRR19973061.2619781/1‑150 (MQ=60)
                                                                                                                                TTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATC                      <  SRR19973061.1317840/150‑1 (MQ=60)
                                                                                                                                      ATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGA                >  SRR19973061.1646961/1‑150 (MQ=60)
                                                                                                                                      ATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGA                >  SRR19973061.3124991/1‑150 (MQ=60)
                                                                                                                                       TATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGAT               >  SRR19973061.2894428/1‑150 (MQ=60)
                                                                                                                                       TATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGAT               <  SRR19973061.789955/150‑1 (MQ=60)
                                                                                                                                        ATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATG              <  SRR19973061.1691552/150‑1 (MQ=60)
                                                                                                                                        ATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATG              >  SRR19973061.2852014/1‑150 (MQ=60)
                                                                                                                                        ATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATG              >  SRR19973061.2852011/1‑150 (MQ=60)
                                                                                                                                             AACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACG         >  SRR19973061.2282775/1‑150 (MQ=60)
                                                                                                                                             AACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACG         >  SRR19973061.2282782/1‑150 (MQ=60)
                                                                                                                                              ACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGG        >  SRR19973061.100808/1‑150 (MQ=60)
                                                                                                                                                CGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCT      <  SRR19973061.679976/150‑1 (MQ=60)
                                                                                                                                                 GACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTG     >  SRR19973061.673444/1‑150 (MQ=60)
                                                                                                                                                  ACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGA    <  SRR19973061.827829/150‑1 (MQ=60)
                                                                                                                                                   CGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGAT   >  SRR19973061.2290284/1‑150 (MQ=60)
                                                                                                                                                   CGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGAT   >  SRR19973061.3712275/1‑150 (MQ=60)
                                                                                                                                                    GTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGATC  >  SRR19973061.3561964/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGATC  >  CP009273/2634269‑2634566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑