Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,634,417 T→G 23.1% K249N (AAA→AAC)  ispG ← 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,634,4170TG23.1% 518.4 / 248.8 334K249N (AAA→AAC) ispG1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (147/110);  new base G (39/38);  total (186/148)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 346 total aligned reads displayed.

TCCATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGATC  >  CP009273/2634268‑2634566
                                                                                                                                                     |                                                                                                                                                     
  cATCGGAGTGATGATATCTTCCAGGAGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACttt                                                                                                                                                     >  2:1090757/1‑150 (MQ=255)
   aTCGGAGTGATGATATCTTCCTGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTg                                                                                                                                                    <  1:1948186/150‑1 (MQ=255)
   aTCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTg                                                                                                                                                    >  2:3983587/1‑150 (MQ=255)
     cGGAGTGATGATATCTTCCTGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAt                                                                                                                                                  <  1:4583506/150‑1 (MQ=255)
        aGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAtctc                                                                                                                                               <  2:2319079/150‑1 (MQ=255)
        aGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAtctc                                                                                                                                               >  2:4938575/1‑150 (MQ=255)
         gTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATctct                                                                                                                                              >  1:249773/1‑150 (MQ=255)
         gTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATctct                                                                                                                                              <  2:1689760/150‑1 (MQ=255)
            atgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCg                                                                                                                                           <  1:3378931/150‑1 (MQ=255)
               atatCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATTAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGAcc                                                                                                                                        <  1:5178737/150‑1 (MQ=255)
                 atCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCgg                                                                                                                                      >  2:4616570/1‑150 (MQ=255)
                   cTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGAt                                                                                                                                    >  2:1472532/1‑150 (MQ=255)
                      ccAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGTCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCgg                                                                                                                                 >  1:3984516/1‑150 (MQ=255)
                       cAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGgc                                                                                                                                >  2:1808209/1‑150 (MQ=255)
                        aGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGgcc                                                                                                                               >  2:3576320/1‑150 (MQ=255)
                        aGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGgcc                                                                                                                               >  2:3714813/1‑150 (MQ=255)
                        aGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGgcc                                                                                                                               >  2:3037506/1‑150 (MQ=255)
                                 tCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGTTACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGAt                                                                                                                      <  1:4501338/150‑1 (MQ=255)
                                  ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATa                                                                                                                     <  2:2227612/150‑1 (MQ=255)
                                  ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATa                                                                                                                     >  2:2866969/1‑150 (MQ=255)
                                  ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATa                                                                                                                     >  2:1344775/1‑150 (MQ=255)
                                   cAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAc                                                                                                                    >  1:4321401/1‑150 (MQ=255)
                                           tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGAGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCg                                                                                                            <  1:4795192/150‑1 (MQ=255)
                                            aaCCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgt                                                                                                           >  2:2436113/1‑150 (MQ=255)
                                            aaCCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgt                                                                                                           >  1:2606735/1‑150 (MQ=255)
                                             aCCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgtg                                                                                                          <  1:2324316/150‑1 (MQ=255)
                                             aCCGTACCGATAACATCAAAGTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgtg                                                                                                          <  2:3403620/150‑1 (MQ=255)
                                               cGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTc                                                                                                        >  1:5159527/1‑150 (MQ=255)
                                                gTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCg                                                                                                       >  1:4679522/1‑150 (MQ=255)
                                                gTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCg                                                                                                       <  2:898961/150‑1 (MQ=255)
                                                  aCCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGcc                                                                                                     >  1:2787554/1‑150 (MQ=255)
                                                  aCCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGcc                                                                                                     <  1:289424/150‑1 (MQ=255)
                                                     gATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGAt                                                                                                  >  1:1859478/1‑150 (MQ=255)
                                                     gATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGAt                                                                                                  >  2:3901068/1‑150 (MQ=255)
                                                      aTAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATg                                                                                                 >  2:2146488/1‑150 (MQ=255)
                                                      aTAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATg                                                                                                 <  2:2800365/150‑1 (MQ=255)
                                                       tAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGc                                                                                                >  1:1198278/1‑150 (MQ=255)
                                                       tAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGc                                                                                                >  2:4883242/1‑150 (MQ=255)
                                                         aCATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCt                                                                                              >  1:1344214/1‑150 (MQ=255)
                                                          cATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCtt                                                                                             <  1:4625260/150‑1 (MQ=255)
                                                          cATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCtt                                                                                             >  1:724276/1‑150 (MQ=255)
                                                           aTCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTc                                                                                            >  1:3352742/1‑150 (MQ=255)
                                                           aTCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTc                                                                                            <  1:2738970/150‑1 (MQ=255)
                                                           aTCAAAGTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTc                                                                                            <  2:633163/150‑1 (MQ=255)
                                                            tCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCa                                                                                           >  2:3522635/1‑150 (MQ=255)
                                                               aaTTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAc                                                                                        >  2:2562265/1‑150 (MQ=255)
                                                                   ccggccgcgACCCGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCTGCGATTTCAACAGATCGAACCCGACTTTGATCTCTTCGCCCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagca                                                                                    <  2:4476116/145‑1 (MQ=255)
                                                                        cgcgAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGa                                                                               >  1:1082974/1‑150 (MQ=255)
                                                                        cgcgAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGa                                                                               <  1:2157943/150‑1 (MQ=255)
                                                                           gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                            >  1:4857675/1‑150 (MQ=255)
                                                                           gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                            >  1:409560/1‑150 (MQ=255)
                                                                           gAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCt                                                                            >  2:1773298/1‑150 (MQ=255)
                                                                            aaCAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTa                                                                           >  1:1660777/1‑150 (MQ=255)
                                                                                ggTCGGGCAGGCGTTGATGTTGATCCCTCGCGAACGGATACGCAGCTATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAcc                                                                       <  1:2402639/150‑1 (MQ=255)
                                                                                   cGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAAt                                                                    <  1:973501/150‑1 (MQ=255)
                                                                                    gggCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATg                                                                   <  2:2068589/150‑1 (MQ=255)
                                                                                     ggCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATgg                                                                  <  2:3194314/150‑1 (MQ=255)
                                                                                          gCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAt                                                             <  1:2217686/150‑1 (MQ=255)
                                                                                              tGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTa                                                         <  2:4134991/150‑1 (MQ=255)
                                                                                                  gTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGc                                                     <  1:1003174/150‑1 (MQ=255)
                                                                                                   ttGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACATAAACCAATGGCGGATTTTACTGcc                                                    >  1:4050274/1‑150 (MQ=255)
                                                                                                     gATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGcccc                                                  >  1:87966/1‑150 (MQ=255)
                                                                                                     gATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGcccc                                                  >  1:1575039/1‑150 (MQ=255)
                                                                                                      aTCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCg                                                 >  2:2016327/1‑150 (MQ=255)
                                                                                                      aTCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCg                                                 >  2:4670002/1‑150 (MQ=255)
                                                                                                         ccTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTg                                              <  1:3572406/150‑1 (MQ=255)
                                                                                                         ccTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTg                                              <  1:1515745/150‑1 (MQ=255)
                                                                                                               gAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGca                                        >  2:3779324/1‑150 (MQ=255)
                                                                                                                 aCGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcacc                                      >  1:1268967/1‑150 (MQ=255)
                                                                                                                  cGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcacca                                     >  1:2472496/1‑150 (MQ=255)
                                                                                                                   ggATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcaccac                                    >  2:568711/1‑150 (MQ=255)
                                                                                                                   ggATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcaccac                                    >  2:3247869/1‑150 (MQ=255)
                                                                                                                    gATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCaccacc                                   <  1:4369087/150‑1 (MQ=255)
                                                                                                                    gATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCaccacc                                   <  1:3911516/150‑1 (MQ=255)
                                                                                                                     aTACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCg                                  >  1:2055945/1‑150 (MQ=255)
                                                                                                                      tACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCgg                                 >  1:613914/1‑150 (MQ=255)
                                                                                                                         gCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCtt                              >  2:5192741/1‑150 (MQ=255)
                                                                                                                           aGCGATTTCAAAATATCGAAACCGACTATGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATGTTACTGCCCCGCTGCGCGCACCACCGGCTTCg                            >  1:1589730/1‑150 (MQ=255)
                                                                                                                              gATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTg                         <  2:1619689/150‑1 (MQ=255)
                                                                                                                                tttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGAt                       <  1:1795533/150‑1 (MQ=255)
                                                                                                                                 ttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATc                      <  2:4691308/150‑1 (MQ=255)
                                                                                                                                 ttCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATc                      <  1:3644273/150‑1 (MQ=255)
                                                                                                                                  tCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATcc                     <  2:4228762/150‑1 (MQ=255)
                                                                                                                                  tCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATcc                     <  2:3281330/150‑1 (MQ=255)
                                                                                                                                    aaaaTATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATcccc                   >  1:4883818/1‑150 (MQ=255)
                                                                                                                                     aaaTATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCa                  <  1:1739603/150‑1 (MQ=255)
                                                                                                                                     aaaTATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCa                  <  2:151266/150‑1 (MQ=255)
                                                                                                                                        tatCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGAt               <  1:3214735/150‑1 (MQ=255)
                                                                                                                                         atCTAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATTCGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAATCCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATg              <  1:469362/150‑1 (MQ=255)
                                                                                                                                           cGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCa            <  1:298749/150‑1 (MQ=255)
                                                                                                                                            gAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCACTGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCaa           >  1:1883849/1‑150 (MQ=255)
                                                                                                                                            gAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCaa           <  2:1198278/150‑1 (MQ=255)
                                                                                                                                              aaCCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACg         <  2:510953/150‑1 (MQ=255)
                                                                                                                                                 cGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCt      >  2:4219246/1‑150 (MQ=255)
                                                                                                                                                  gACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTg     <  2:4739779/150‑1 (MQ=255)
                                                                                                                                                   aCTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTga    >  2:3135384/1‑150 (MQ=255)
                                                                                                                                                    cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTgat   >  2:4409778/1‑150 (MQ=255)
                                                                                                                                                     tttGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTgatc  <  2:3457028/150‑1 (MQ=255)
                                                                                                                                                     tttGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTgatc  >  2:3382584/1‑150 (MQ=255)
                                                                                                                                                     tttGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTgatc  <  1:4137925/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGATC  >  CP009273/2634268‑2634566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A