Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,634,417 T→G 16.8% K249N (AAA→AAC)  ispG ← 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,634,4170TG16.8% 434.9 / 105.9 213K249N (AAA→AAC) ispG1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (108/69);  new base G (25/11);  total (133/80)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 222 total aligned reads displayed.

CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGA  >  CP009273/2634270‑2634564
                                                                                                                                                   |                                                                                                                                                   
cATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACt                                                                                                                                                     >  2:706069/1‑148 (MQ=255)
cATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACt                                                                                                                                                     <  1:1584318/148‑1 (MQ=255)
cATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACt                                                                                                                                                     <  2:429085/148‑1 (MQ=255)
 aTCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACtt                                                                                                                                                    <  2:1508738/148‑1 (MQ=255)
    ggtgtgtTGATATCTTCCAGGGGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACTGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCTGCGATTTCAAAATATCGAAACCGACTTTGa                                                                                                                                                 <  1:759366/145‑1 (MQ=255)
    ggAGTGATTATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGa                                                                                                                                                 <  1:2975418/148‑1 (MQ=255)
     gAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAt                                                                                                                                                >  1:1351098/1‑148 (MQ=255)
     gAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAt                                                                                                                                                >  1:3309456/1‑148 (MQ=255)
        tgatgaTATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGAtctc                                                                                                                                             <  2:2003835/148‑1 (MQ=255)
         gatgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATctct                                                                                                                                            >  1:683406/1‑148 (MQ=255)
         gatgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATctct                                                                                                                                            >  1:1784227/1‑148 (MQ=255)
         gatgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATctct                                                                                                                                            >  2:2990075/1‑148 (MQ=255)
          atgatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCtt                                                                                                                                           >  2:3151920/1‑148 (MQ=255)
            gatATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCg                                                                                                                                         <  1:3182266/148‑1 (MQ=255)
               atCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGAcc                                                                                                                                      >  2:280958/1‑148 (MQ=255)
               atCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGAcc                                                                                                                                      >  2:1729517/1‑148 (MQ=255)
                tCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCg                                                                                                                                     <  2:1869793/148‑1 (MQ=255)
                 cTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCgg                                                                                                                                    >  1:3605307/1‑148 (MQ=255)
                  ttCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGa                                                                                                                                   <  1:2983806/148‑1 (MQ=255)
                   tCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGAt                                                                                                                                  >  2:3490589/1‑148 (MQ=255)
                      aGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCgg                                                                                                                               >  2:3421206/1‑148 (MQ=255)
                        gCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGgcc                                                                                                                             >  1:2670625/1‑148 (MQ=255)
                                ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGa                                                                                                                     >  1:3025961/1‑148 (MQ=255)
                                ccAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGa                                                                                                                     <  2:595057/148‑1 (MQ=255)
                                   gcgcGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAc                                                                                                                  >  2:952439/1‑148 (MQ=255)
                                      cgTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATAcgcg                                                                                                               >  2:91695/1‑148 (MQ=255)
                                         tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAg                                                                                                            <  1:2082551/148‑1 (MQ=255)
                                         tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAg                                                                                                            <  2:1376745/148‑1 (MQ=255)
                                         tAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAg                                                                                                            >  2:2247511/1‑148 (MQ=255)
                                            ccGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCgt                                                                                                         <  2:1784227/148‑1 (MQ=255)
                                                aCCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCg                                                                                                     >  2:3003220/1‑148 (MQ=255)
                                                  cGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGcc                                                                                                   >  1:72027/1‑148 (MQ=255)
                                                   gATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCg                                                                                                  <  1:2752626/148‑1 (MQ=255)
                                                   gATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCg                                                                                                  >  2:2326184/1‑148 (MQ=255)
                                                      aaCATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCTATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATg                                                                                               <  1:1389286/148‑1 (MQ=255)
                                                             aaTTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAg                                                                                        <  2:3125583/148‑1 (MQ=255)
                                                               ttCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAc                                                                                      >  2:2725225/1‑148 (MQ=255)
                                                                tCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAca                                                                                     >  2:902815/1‑148 (MQ=255)
                                                                      cgcgAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGAcagcagca                                                                               <  1:1974075/148‑1 (MQ=255)
                                                                        cgAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGa                                                                             <  1:3003220/148‑1 (MQ=255)
                                                                          aaCAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGAcc                                                                           <  2:2853384/148‑1 (MQ=255)
                                                                          aaCAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGAcc                                                                           <  2:3243688/148‑1 (MQ=255)
                                                                            cAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTa                                                                         >  1:2307616/1‑148 (MQ=255)
                                                                            cAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTa                                                                         >  1:3224100/1‑148 (MQ=255)
                                                                              ggTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTaaa                                                                       <  1:615189/148‑1 (MQ=255)
                                                                               gTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCGAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAc                                                                      >  1:777176/1‑148 (MQ=255)
                                                                               gTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAc                                                                      >  1:2847596/1‑148 (MQ=255)
                                                                               gTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAAc                                                                      >  1:2847256/1‑148 (MQ=255)
                                                                                 cGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCa                                                                    <  1:591435/148‑1 (MQ=255)
                                                                                   gtcAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAAt                                                                  <  1:2494082/146‑1 (MQ=255)
                                                                                   ggCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAAt                                                                  <  1:2850025/148‑1 (MQ=255)
                                                                                       ggCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCg                                                              <  1:486195/148‑1 (MQ=255)
                                                                                        gCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCgg                                                             >  1:2057092/1‑148 (MQ=255)
                                                                                          gATGAAGTGGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGACACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGAGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCACTGGCGGAt                                                           <  2:3101285/148‑1 (MQ=255)
                                                                                            tGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAttt                                                         >  2:689191/1‑148 (MQ=255)
                                                                                            tGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAttt                                                         <  1:3421206/148‑1 (MQ=255)
                                                                                             gAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAtttt                                                        >  2:2786741/1‑148 (MQ=255)
                                                                                             gAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGAtttt                                                        >  2:874040/1‑148 (MQ=255)
                                                                                              aaGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTa                                                       >  1:1508424/1‑148 (MQ=255)
                                                                                              aaGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTa                                                       <  2:1847326/148‑1 (MQ=255)
                                                                                              aaGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTa                                                       >  1:3408298/1‑148 (MQ=255)
                                                                                               aGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTAc                                                      >  2:1559154/1‑148 (MQ=255)
                                                                                                 ttGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTg                                                    >  2:3799585/1‑148 (MQ=255)
                                                                                                   gATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGcc                                                  <  1:2586344/148‑1 (MQ=255)
                                                                                                      cccTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCg                                               >  2:1150674/1‑148 (MQ=255)
                                                                                                       ccTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGc                                              <  1:2175563/148‑1 (MQ=255)
                                                                                                         tCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTg                                            >  1:1068415/1‑148 (MQ=255)
                                                                                                         tCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTg                                            >  1:3492033/1‑148 (MQ=255)
                                                                                                         tCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTg                                            >  1:3492658/1‑148 (MQ=255)
                                                                                                               aCGGATACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGca                                      >  1:1383375/1‑148 (MQ=255)
                                                                                                                   aTACGCAGCGATTTCAAAATATCGAAACCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGcaccac                                  >  1:2124198/1‑148 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCt                             <  2:3605307/148‑1 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCt                             <  2:2910520/148‑1 (MQ=255)
                                                                                                                        cAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCt                             <  1:3138743/148‑1 (MQ=255)
                                                                                                                              tttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTg                       >  1:2257911/1‑148 (MQ=255)
                                                                                                                              tttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTg                       >  2:1903730/1‑148 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGa                      >  2:1169449/1‑148 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGa                      >  1:1156817/1‑148 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGa                      >  2:1170623/1‑148 (MQ=255)
                                                                                                                               ttCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGa                      >  1:2728532/1‑148 (MQ=255)
                                                                                                                                 cAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATc                    <  2:494134/148‑1 (MQ=255)
                                                                                                                                   aaaTATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATccc                  <  1:416289/148‑1 (MQ=255)
                                                                                                                                      tatCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAg               >  2:2555227/1‑148 (MQ=255)
                                                                                                                                      tatCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAg               <  1:706069/148‑1 (MQ=255)
                                                                                                                                      tatCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAg               >  1:803943/1‑148 (MQ=255)
                                                                                                                                       atCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGa              <  1:263684/148‑1 (MQ=255)
                                                                                                                                            aaCCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCaa         <  1:1681830/148‑1 (MQ=255)
                                                                                                                                             aCCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAAc        >  1:2712031/1‑148 (MQ=255)
                                                                                                                                             aCCGACGTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAAc        >  2:2136126/1‑148 (MQ=255)
                                                                                                                                               cGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACgg      <  1:2218376/148‑1 (MQ=255)
                                                                                                                                               cGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACgg      <  2:3303141/148‑1 (MQ=255)
                                                                                                                                               cGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACgg      >  1:3816193/1‑148 (MQ=255)
                                                                                                                                                 aCTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCt    >  2:3072342/1‑148 (MQ=255)
                                                                                                                                                 aCTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCt    >  2:2534566/1‑148 (MQ=255)
                                                                                                                                                  cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAActgctg   >  1:3647672/1‑148 (MQ=255)
                                                                                                                                                  cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTg   >  2:1038848/1‑148 (MQ=255)
                                                                                                                                                  cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTg   <  2:2307312/148‑1 (MQ=255)
                                                                                                                                                  cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTg   <  1:3741756/148‑1 (MQ=255)
                                                                                                                                                  cTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTg   >  1:2414464/1‑148 (MQ=255)
                                                                                                                                                   tttGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTga  <  1:910255/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATCGGAGTGATGATATCTTCCAGGCGTTGCTCCAGCGCGTTAACCGTACCGATAACATCAAATTCCTGACGCGAACAGGTCGGGCAGGCGATGAAGTTGATCCCTCGCGAACGGATACGCAGCGATTTCAAAATATCGAAACCGACTTTGATCTCTTCGACCGGATCGGCCGCCAGCGATACGCGCAGCGTGTCGCCGATGCCTTCAGACAGCAGCAGACCTAAACCAATGGCGGATTTTACTGCCCCGCTGCGCGCACCACCGGCTTCGGTGATCCCCAGATGCAACGGCTGA  >  CP009273/2634270‑2634564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A