Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 580,366 G→T 100% R242S (CGC→AGC)  ompT ← DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273580,3660GT96.6% 700.9 / 8.9 227R242S (CGC→AGC) ompTDLP12 prophage; outer membrane protease VII (outer membrane protein 3b)
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base T (133/85);  total (137/90)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

TATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG  >  CP009273/580219‑580514
                                                                                                                                                   |                                                                                                                                                    
tattatTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTAt                                                                                                                                                    >  2:153718/1‑150 (MQ=255)
 attattGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCtata                                                                                                                                                   >  2:3693965/1‑150 (MQ=255)
    attGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGt                                                                                                                                                >  1:2770651/1‑150 (MQ=255)
      tgtgATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGa                                                                                                                                              >  1:1412169/1‑150 (MQ=255)
        tgATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGAtt                                                                                                                                            <  1:1126789/150‑1 (MQ=255)
              tAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCttttt                                                                                                                                      >  1:492327/1‑150 (MQ=255)
               aaaGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTc                                                                                                                                     >  1:251771/1‑150 (MQ=255)
                   tGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCgg                                                                                                                                 >  1:4122830/1‑150 (MQ=255)
                    gAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCggg                                                                                                                                <  2:3346958/150‑1 (MQ=255)
                    gAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCggg                                                                                                                                <  2:1345927/150‑1 (MQ=255)
                    gAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCggg                                                                                                                                >  2:77608/1‑150 (MQ=255)
                     aaGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGt                                                                                                                               <  2:3683587/150‑1 (MQ=255)
                     aaGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGt                                                                                                                               <  2:1454055/150‑1 (MQ=255)
                      aGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTc                                                                                                                              <  2:3938588/150‑1 (MQ=255)
                       gTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGTGTCa                                                                                                                             >  2:3371277/1‑150 (MQ=255)
                          ttACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAg                                                                                                                          >  1:4614441/1‑150 (MQ=255)
                          ttACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAg                                                                                                                          >  1:3420038/1‑150 (MQ=255)
                           tACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAgt                                                                                                                         >  2:304140/1‑150 (MQ=255)
                            aCCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAgtg                                                                                                                        >  2:3730727/1‑150 (MQ=255)
                             ccTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAAGTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAgtgt                                                                                                                       >  2:2986359/1‑150 (MQ=255)
                                tttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCa                                                                                                                    >  2:1818387/1‑150 (MQ=255)
                                tttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCa                                                                                                                    >  2:27487/1‑150 (MQ=255)
                                  tttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATc                                                                                                                  >  2:498750/1‑150 (MQ=255)
                                   ttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTCTAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCg                                                                                                                 >  1:1939992/1‑150 (MQ=255)
                                   ttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGtt                                                                                                                      <  1:4409909/145‑1 (MQ=255)
                                    tATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGt                                                                                                                >  2:1778568/1‑150 (MQ=255)
                                    tATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGt                                                                                                                >  1:952678/1‑150 (MQ=255)
                                      ttCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTa                                                                                                              >  2:3253734/1‑150 (MQ=255)
                                        cGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATc                                                                                                            >  1:2409273/1‑150 (MQ=255)
                                        cGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCAGAGTGTTCATCGTTATc                                                                                                            <  1:1043486/150‑1 (MQ=255)
                                         gTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCa                                                                                                           >  2:5145638/1‑150 (MQ=255)
                                            aCCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAgat                                                                                                        >  2:2510246/1‑150 (MQ=255)
                                             cccGATTCCATGCGCCTTCAACATAAACTTTAGCGTTAGGTGTGACGTAATAACCTGCATAGACTGCAACAGAATAGAAATTTTGGTCTTTGACATTACTGCTATAAGAGATTCTTTTTACCGGGTCATAGTGTTCATCGTTATCAgatg                                                                                                       >  2:1092795/1‑150 (MQ=255)
                                              ccGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAgatga                                                                                                      <  1:2643566/150‑1 (MQ=255)
                                                gATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGAtt                                                                                                    >  2:2034736/1‑150 (MQ=255)
                                                gATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGAtt                                                                                                    >  1:664139/1‑150 (MQ=255)
                                                 aTTACATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTc                                                                                                   >  1:4331310/1‑150 (MQ=255)
                                                    ccATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAc                                                                                                <  1:1254709/150‑1 (MQ=255)
                                                     cATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAcc                                                                                               <  2:2344808/150‑1 (MQ=255)
                                                     cATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAcc                                                                                               >  1:848950/1‑150 (MQ=255)
                                                      aTGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAccc                                                                                              >  2:81087/1‑150 (MQ=255)
                                                      aTGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAccc                                                                                              >  1:3956715/1‑150 (MQ=255)
                                                      aTGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAccc                                                                                              <  1:498750/150‑1 (MQ=255)
                                                       tGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCa                                                                                             >  2:4662880/1‑150 (MQ=255)
                                                          gcCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGcc                                                                                          >  2:1917022/1‑150 (MQ=255)
                                                             ttCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACGTTACTGCTATAAGTTATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCt                                                                                       <  1:1748404/150‑1 (MQ=255)
                                                                   aTAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAttt                                                                                 >  2:4546221/1‑150 (MQ=255)
                                                                    tAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTa                                                                                <  2:2114044/150‑1 (MQ=255)
                                                                    tAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTa                                                                                <  2:1896928/150‑1 (MQ=255)
                                                                      aaCTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaaa                                                                              >  2:4323172/1‑150 (MQ=255)
                                                                        cTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAAtg                                                                            >  2:4346203/1‑150 (MQ=255)
                                                                            tGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGcc                                                                        >  2:2835770/1‑150 (MQ=255)
                                                                              cGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAc                                                                      >  1:1549787/1‑150 (MQ=255)
                                                                              cGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAc                                                                      >  1:4745713/1‑150 (MQ=255)
                                                                               gTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAcc                                                                     <  1:1971990/150‑1 (MQ=255)
                                                                                ttAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCg                                                                    >  2:1343847/1‑150 (MQ=255)
                                                                                ttAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCg                                                                    <  1:1156390/150‑1 (MQ=255)
                                                                                 tAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGa                                                                   >  1:2424162/1‑150 (MQ=255)
                                                                                 tAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGa                                                                   <  1:2844595/150‑1 (MQ=255)
                                                                                     tgtgACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTc                                                               <  1:4662880/150‑1 (MQ=255)
                                                                                      gtgACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCa                                                              <  2:1648432/150‑1 (MQ=255)
                                                                                      gtgACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCa                                                              >  2:3415060/1‑150 (MQ=255)
                                                                                       tgACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCaa                                                             <  1:51643/150‑1 (MQ=255)
                                                                                         aCGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCaaaa                                                           <  1:5143299/150‑1 (MQ=255)
                                                                                          cttaataaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAt                                                          >  1:4480654/3‑150 (MQ=255)
                                                                                            taataaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCGCCGAGTTCAAAATCt                                                        >  2:109570/1‑150 (MQ=255)
                                                                                             aataaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCtt                                                       <  1:3856400/150‑1 (MQ=255)
                                                                                               taaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCa                                                     >  2:3916193/1‑150 (MQ=255)
                                                                                                aaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCAt                                                    >  1:3385974/1‑150 (MQ=255)
                                                                                                 aCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATa                                                   <  2:3808491/150‑1 (MQ=255)
                                                                                                   cTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAAc                                                 <  1:4202429/150‑1 (MQ=255)
                                                                                                      cATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGAt                                              <  2:752761/150‑1 (MQ=255)
                                                                                                       aTTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATa                                             >  1:769638/1‑150 (MQ=255)
                                                                                                        ttGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCCGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATaa                                            >  2:3432494/1‑150 (MQ=255)
                                                                                                        ttGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATaa                                            >  1:3184860/1‑150 (MQ=255)
                                                                                                         tGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGCTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAAc                                           <  1:1777712/150‑1 (MQ=255)
                                                                                                          gACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACt                                          >  1:1521846/1‑150 (MQ=255)
                                                                                                           aCTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACtt                                         >  2:593628/1‑150 (MQ=255)
                                                                                                           aCTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACtt                                         >  2:2281203/1‑150 (MQ=255)
                                                                                                              gCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCa                                      <  2:2134930/150‑1 (MQ=255)
                                                                                                                 aCAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTc                                   >  1:3723734/1‑150 (MQ=255)
                                                                                                                   aGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCaa                                 <  2:3681612/150‑1 (MQ=255)
                                                                                                                    gAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAg                                >  2:409573/1‑150 (MQ=255)
                                                                                                                       tAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                             <  1:519137/150‑1 (MQ=255)
                                                                                                                       tAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                             <  1:1859981/150‑1 (MQ=255)
                                                                                                                       tAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                             >  1:2915452/1‑150 (MQ=255)
                                                                                                                       tAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                             >  1:2915582/1‑150 (MQ=255)
                                                                                                                         gTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAAt                           <  2:2770651/150‑1 (MQ=255)
                                                                                                                           aaTTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGt                         >  1:873098/1‑150 (MQ=255)
                                                                                                                              tttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCACTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAgg                      >  1:414768/1‑150 (MQ=255)
                                                                                                                               ttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAggg                     >  1:1680789/1‑150 (MQ=255)
                                                                                                                                 ggTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCa                   <  1:408971/150‑1 (MQ=255)
                                                                                                                                    cTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCAttt                >  2:1936833/1‑150 (MQ=255)
                                                                                                                                      ttGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTa              >  1:2385165/1‑150 (MQ=255)
                                                                                                                                       tGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTaa             >  1:659207/1‑150 (MQ=255)
                                                                                                                                         aCCTTACTGCTATGAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTaaaa           >  2:550226/1‑150 (MQ=255)
                                                                                                                                             tACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACgtt       >  2:594930/1‑150 (MQ=255)
                                                                                                                                              aCTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACgttg      <  1:2491583/150‑1 (MQ=255)
                                                                                                                                              aCTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACgttg      <  2:452055/150‑1 (MQ=255)
                                                                                                                                               cTGCTATACGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACgttgt     <  2:4561737/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG  >  CP009273/580219‑580514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  CP009273/580217‑580515
                                                                                                                                                     |                                                                                                                                                     
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCT                                                                                                                                                       >  SRR19973063.2131941/1‑150 (MQ=60)
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCT                                                                                                                                                       >  SRR19973063.4722231/1‑150 (MQ=60)
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCAGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCT                                                                                                                                                       >  SRR19973063.4722209/1‑150 (MQ=60)
   ATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATA                                                                                                                                                    <  SRR19973063.3755219/150‑1 (MQ=60)
   ATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATA                                                                                                                                                    >  SRR19973063.85256/1‑150 (MQ=60)
      ATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGT                                                                                                                                                 >  SRR19973063.2779498/1‑150 (MQ=60)
      ATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGT                                                                                                                                                 >  SRR19973063.2779584/1‑150 (MQ=60)
        TGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGA                                                                                                                                               >  SRR19973063.1416225/1‑150 (MQ=60)
        TGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGA                                                                                                                                               >  SRR19973063.1416454/1‑150 (MQ=60)
          TGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATT                                                                                                                                             <  SRR19973063.1130209/150‑1 (MQ=60)
                TAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTT                                                                                                                                       >  SRR19973063.493742/1‑150 (MQ=60)
                TAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTT                                                                                                                                       <  SRR19973063.4918695/150‑1 (MQ=60)
                TAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTT                                                                                                                                       >  SRR19973063.3093961/1‑150 (MQ=60)
                 AAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTC                                                                                                                                      >  SRR19973063.252534/1‑150 (MQ=60)
                 AAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTC                                                                                                                                      >  SRR19973063.252501/1‑150 (MQ=60)
                 AAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTC                                                                                                                                      >  SRR19973063.1838063/1‑150 (MQ=60)
                     TGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGG                                                                                                                                  >  SRR19973063.4136027/1‑150 (MQ=60)
                      GAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGG                                                                                                                                 >  SRR19973063.328189/1‑150 (MQ=60)
                            TTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAG                                                                                                                           >  SRR19973063.3430922/1‑150 (MQ=60)
                            TTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAG                                                                                                                           >  SRR19973063.3427951/1‑150 (MQ=60)
                            TTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAG                                                                                                                           >  SRR19973063.4629192/1‑150 (MQ=60)
                             TACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGT                                                                                                                          >  SRR19973063.891561/1‑150 (MQ=60)
                                gatctttagTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTT                                                                                                                       <  SRR19973063.4424050/141‑1 (MQ=60)
                                     TTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTCTAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCG                                                                                                                  >  SRR19973063.1946061/1‑150 (MQ=60)
                                      TATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGT                                                                                                                 >  SRR19973063.1403618/1‑150 (MQ=60)
                                      TATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGT                                                                                                                 >  SRR19973063.955540/1‑150 (MQ=60)
                                         TCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTAT                                                                                                              >  SRR19973063.4408229/1‑150 (MQ=60)
                                          CGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATC                                                                                                             >  SRR19973063.2416979/1‑150 (MQ=60)
                                          CGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCAGAGTGTTCATCGTTATC                                                                                                             <  SRR19973063.1046647/150‑1 (MQ=60)
                                          CGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGGCATAGTGTTCATCGTTATC                                                                                                             <  SRR19973063.3632242/150‑1 (MQ=60)
                                            TAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAG                                                                                                           <  SRR19973063.2462379/150‑1 (MQ=60)
                                            TAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAG                                                                                                           >  SRR19973063.2566083/1‑150 (MQ=60)
                                                CCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGA                                                                                                       >  SRR19973063.3904105/1‑150 (MQ=60)
                                                CCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGA                                                                                                       <  SRR19973063.2652014/150‑1 (MQ=60)
                                                  GATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATT                                                                                                     >  SRR19973063.2896344/1‑150 (MQ=60)
                                                  GATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATT                                                                                                     >  SRR19973063.666082/1‑150 (MQ=60)
                                                  GATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTACCGGGTCATAGTGTTCATCGTTATCAGATGATT                                                                                                     >  SRR19973063.666016/1‑150 (MQ=60)
                                                   ATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973063.4345042/1‑150 (MQ=60)
                                                   ATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973063.2513709/1‑150 (MQ=60)
                                                   ATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973063.4345038/1‑150 (MQ=60)
                                                   ATTACATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973063.4345112/1‑150 (MQ=60)
                                                    TTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCC                                                                                                   >  SRR19973063.1064185/1‑150 (MQ=60)
                                                      CCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAC                                                                                                 <  SRR19973063.1258445/150‑1 (MQ=60)
                                                      CCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGAGTCCAC                                                                                                 >  SRR19973063.3086026/1‑150 (MQ=60)
                                                       CATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACC                                                                                                >  SRR19973063.851401/1‑150 (MQ=60)
                                                        ATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCC                                                                                               >  SRR19973063.3969374/1‑150 (MQ=60)
                                                        ATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCC                                                                                               <  SRR19973063.500184/150‑1 (MQ=60)
                                                        ATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCC                                                                                               <  SRR19973063.701067/150‑1 (MQ=60)
                                                           CGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGC                                                                                            >  SRR19973063.4660859/1‑150 (MQ=60)
                                                               TTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTTGTCTTTGACGTTACTGCTATAAGTTATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCT                                                                                        <  SRR19973063.1753762/150‑1 (MQ=60)
                                                                   ACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAT                                                                                    >  SRR19973063.4502255/1‑150 (MQ=60)
                                                                             TTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGC                                                                          <  SRR19973063.1138161/150‑1 (MQ=60)
                                                                              TGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCC                                                                         <  SRR19973063.4539055/150‑1 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973063.1554536/1‑150 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973063.1554514/1‑150 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973063.4760822/1‑150 (MQ=60)
                                                                                 GTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACC                                                                      >  SRR19973063.4164584/1‑150 (MQ=60)
                                                                                 GTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACC                                                                      <  SRR19973063.1978157/150‑1 (MQ=60)
                                                                                  TTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCG                                                                     <  SRR19973063.1159878/150‑1 (MQ=60)
                                                                                   TAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGA                                                                    <  SRR19973063.415078/150‑1 (MQ=60)
                                                                                   TAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGA                                                                    <  SRR19973063.2844876/150‑1 (MQ=60)
                                                                                   TAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGA                                                                    >  SRR19973063.2431911/1‑150 (MQ=60)
                                                                                   TAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGA                                                                    <  SRR19973063.2853721/150‑1 (MQ=60)
                                                                                    AGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAG                                                                   <  SRR19973063.4993088/150‑1 (MQ=60)
                                                                                       TGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTC                                                                <  SRR19973063.4677752/150‑1 (MQ=60)
                                                                                         TGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAA                                                              <  SRR19973063.51828/150‑1 (MQ=60)
                                                                                           ACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAA                                                            <  SRR19973063.5159852/150‑1 (MQ=60)
                                                                                           ACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAA                                                            <  SRR19973063.2072595/150‑1 (MQ=60)
                                                                                            CTTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAT                                                           >  SRR19973063.4495023/1‑150 (MQ=60)
                                                                                            CTTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAT                                                           >  SRR19973063.4495022/1‑150 (MQ=60)
                                                                                            CGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAT                                                           <  SRR19973063.1627546/150‑1 (MQ=60)
                                                                                             GTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATC                                                          <  SRR19973063.598346/150‑1 (MQ=60)
                                                                                             GTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATC                                                          >  SRR19973063.4103187/1‑150 (MQ=60)
                                                                                             GTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATC                                                          >  SRR19973063.1604267/1‑150 (MQ=60)
                                                                                              TAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCT                                                         <  SRR19973063.2785117/150‑1 (MQ=60)
                                                                                               AATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTT                                                        <  SRR19973063.3868667/150‑1 (MQ=60)
                                                                                                 TAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCA                                                      >  SRR19973063.1855267/1‑150 (MQ=60)
                                                                                                  AACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCAT                                                     >  SRR19973063.3396762/1‑150 (MQ=60)
                                                                                                     CTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAAC                                                  <  SRR19973063.4215839/150‑1 (MQ=60)
                                                                                                      TGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACG                                                 <  SRR19973063.1700425/150‑1 (MQ=60)
                                                                                                         ATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATA                                              >  SRR19973063.771881/1‑150 (MQ=60)
                                                                                                          TTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAA                                             >  SRR19973063.3195099/1‑150 (MQ=60)
                                                                                                          TTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAA                                             <  SRR19973063.1909187/150‑1 (MQ=60)
                                                                                                           TGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGCTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAAC                                            <  SRR19973063.1783201/150‑1 (MQ=60)
                                                                                                            GACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACT                                           >  SRR19973063.1526514/1‑150 (MQ=60)
                                                                                                            GACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACT                                           <  SRR19973063.1956333/150‑1 (MQ=60)
                                                                                                             TCTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTT                                          >  SRR19973063.4684982/1‑150 (MQ=60)
                                                                                                             ACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTT                                          <  SRR19973063.3682455/150‑1 (MQ=60)
                                                                                                                GCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCA                                       <  SRR19973063.1453028/150‑1 (MQ=60)
                                                                                                                 CAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAG                                      >  SRR19973063.4132363/1‑150 (MQ=60)
                                                                                                                   ACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTC                                    >  SRR19973063.3735636/1‑150 (MQ=60)
                                                                                                                    CAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCA                                   <  SRR19973063.2252218/150‑1 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              <  SRR19973063.520641/150‑1 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              <  SRR19973063.1865789/150‑1 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              <  SRR19973063.520652/150‑1 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              >  SRR19973063.2924751/1‑150 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              >  SRR19973063.2924875/1‑150 (MQ=60)
                                                                                                                         TAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCA                              >  SRR19973063.2924881/1‑150 (MQ=60)
                                                                                                                          AGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAA                             <  SRR19973063.1767671/150‑1 (MQ=60)
                                                                                                                           GTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAAT                            <  SRR19973063.5175910/150‑1 (MQ=60)
                                                                                                                             AATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGT                          >  SRR19973063.875623/1‑150 (MQ=60)
                                                                                                                             AATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGT                          >  SRR19973063.875608/1‑150 (MQ=60)
                                                                                                                              ATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTA                         <  SRR19973063.2404343/150‑1 (MQ=60)
                                                                                                                                TTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCACTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGG                       >  SRR19973063.415932/1‑150 (MQ=60)
                                                                                                                                 TTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGG                      <  SRR19973063.1249857/150‑1 (MQ=60)
                                                                                                                                 TTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGG                      >  SRR19973063.1685911/1‑150 (MQ=60)
                                                                                                                                   GGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCA                    <  SRR19973063.410122/150‑1 (MQ=60)
                                                                                                                                     TCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATT                  >  SRR19973063.4016303/1‑150 (MQ=60)
                                                                                                                                        TTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTA               >  SRR19973063.2392803/1‑150 (MQ=60)
                                                                                                                                         TGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAA              >  SRR19973063.2425193/1‑150 (MQ=60)
                                                                                                                                         TGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAA              >  SRR19973063.79704/1‑150 (MQ=60)
                                                                                                                                         TGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAA              >  SRR19973063.661137/1‑150 (MQ=60)
                                                                                                                                            CCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAAC           >  SRR19973063.4846031/1‑150 (MQ=60)
                                                                                                                                             CTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACG          >  SRR19973063.693730/1‑150 (MQ=60)
                                                                                                                                                ACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTG       <  SRR19973063.2499536/150‑1 (MQ=60)
                                                                                                                                                ACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTG       >  SRR19973063.5066335/1‑150 (MQ=60)
                                                                                                                                                 CTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGT      >  SRR19973063.5163690/1‑150 (MQ=60)
                                                                                                                                                    CTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG   <  SRR19973063.3829107/150‑1 (MQ=60)
                                                                                                                                                    CTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG   <  SRR19973063.42460/150‑1 (MQ=60)
                                                                                                                                                     TATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACATCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  SRR19973063.3621355/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  CP009273/580217‑580515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑