Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 580,366 G→T 100% R242S (CGC→AGC)  ompT ← DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273580,3660GT99.1% 768.7 / ‑1.9 234R242S (CGC→AGC) ompTDLP12 prophage; outer membrane protease VII (outer membrane protein 3b)
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (128/104);  total (128/106)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

TTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTT  >  CP009273/580221‑580509
                                                                                                                                                 |                                                                                                                                               
      tgATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGa                                                                                                                                         <  1:532706/148‑1 (MQ=255)
           aTAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCtt                                                                                                                                    <  1:827156/148‑1 (MQ=255)
            tAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCttt                                                                                                                                   >  1:480672/1‑148 (MQ=255)
              aaGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCttttt                                                                                                                                 <  1:2080592/148‑1 (MQ=255)
              aaGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCttttt                                                                                                                                 <  1:538411/148‑1 (MQ=255)
               aGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTc                                                                                                                                <  2:2269257/148‑1 (MQ=255)
                  gAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCg                                                                                                                             <  1:2860319/148‑1 (MQ=255)
                   aaGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGNCTTCAACATAAACTTTTGCGTTAGNTGNGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTNTGACCTTACTGCTATAAGTGATTCTTTTTCCCgg                                                                                                                            >  1:3401560/1‑148 (MQ=255)
                     gTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGt                                                                                                                          <  2:1473592/148‑1 (MQ=255)
                      tATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTc                                                                                                                         >  1:1465985/1‑148 (MQ=255)
                      tATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTc                                                                                                                         >  2:2378682/1‑148 (MQ=255)
                        ttACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCAt                                                                                                                       <  1:3195658/148‑1 (MQ=255)
                         tACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATa                                                                                                                      >  1:1765424/1‑148 (MQ=255)
                         tACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATa                                                                                                                      <  2:3186600/148‑1 (MQ=255)
                           ccTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAgt                                                                                                                    >  1:3145157/1‑148 (MQ=255)
                           ccTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAgt                                                                                                                    >  2:35379/1‑148 (MQ=255)
                             ttttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAgtgt                                                                                                                  <  1:2133642/148‑1 (MQ=255)
                              tttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGtt                                                                                                                 <  2:1832299/148‑1 (MQ=255)
                              tttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGtt                                                                                                                 <  2:54800/148‑1 (MQ=255)
                               ttttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTc                                                                                                                >  2:2509504/1‑148 (MQ=255)
                                tttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCa                                                                                                               <  1:1960369/148‑1 (MQ=255)
                                tttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCa                                                                                                               >  2:114221/1‑148 (MQ=255)
                                 ttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCAt                                                                                                              <  2:3248488/148‑1 (MQ=255)
                                 ttATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCAt                                                                                                              <  2:1952071/148‑1 (MQ=255)
                                    ttCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGt                                                                                                           >  2:2238040/1‑148 (MQ=255)
                                         aaCCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCa                                                                                                      >  2:2955809/1‑148 (MQ=255)
                                           cccGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAga                                                                                                    <  1:223508/148‑1 (MQ=255)
                                           cccGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAga                                                                                                    >  2:2760115/1‑148 (MQ=255)
                                            ccGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAgat                                                                                                   >  2:848771/1‑148 (MQ=255)
                                              gATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAgatga                                                                                                 >  1:435371/1‑148 (MQ=255)
                                                ttCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGAtt                                                                                               <  1:695185/148‑1 (MQ=255)
                                                ttCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGAtt                                                                                               >  2:1301880/1‑148 (MQ=255)
                                                 tCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACATAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGATCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTc                                                                                              >  1:2998233/1‑148 (MQ=255)
                                                    aTGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAc                                                                                           >  1:1511618/1‑148 (MQ=255)
                                                     tGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAcc                                                                                          <  1:855202/148‑1 (MQ=255)
                                                      gcgcCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAccc                                                                                         >  1:64745/1‑148 (MQ=255)
                                                       cgcCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCa                                                                                        >  1:594016/1‑148 (MQ=255)
                                                        gcCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGGCATAGTGTTCATCGTTATCAGATGATTCCACCCAg                                                                                       <  1:1639185/148‑1 (MQ=255)
                                                          cTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGcc                                                                                     >  1:1177979/1‑148 (MQ=255)
                                                            tCAACATAAACTTTAGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCACGGGTCATAGTGTTCATCGTTATCAGATCATTCCACCCAGCCGc                                                                                   >  1:50162/1‑148 (MQ=255)
                                                               aCATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGt                                                                                <  1:1806221/148‑1 (MQ=255)
                                                                cATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTa                                                                               <  1:2527463/148‑1 (MQ=255)
                                                                 aTAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAt                                                                              <  1:952219/148‑1 (MQ=255)
                                                                   aaaCTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAttt                                                                            >  1:2619829/1‑148 (MQ=255)
                                                                    aaCTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTa                                                                           >  1:1775806/1‑148 (MQ=255)
                                                                     aCTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaa                                                                          <  1:2244948/148‑1 (MQ=255)
                                                                     aCTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaa                                                                          <  1:185617/148‑1 (MQ=255)
                                                                      cTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaaa                                                                         <  1:1360271/148‑1 (MQ=255)
                                                                      cTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaaa                                                                         <  2:2578314/148‑1 (MQ=255)
                                                                      cTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGGCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTaaa                                                                         <  2:1326751/148‑1 (MQ=255)
                                                                       ttttGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAAt                                                                        >  2:1181471/1‑148 (MQ=255)
                                                                              ttAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAc                                                                 >  1:1289043/1‑148 (MQ=255)
                                                                               tAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAcc                                                                >  2:2856640/1‑148 (MQ=255)
                                                                                aGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCg                                                               <  1:793837/148‑1 (MQ=255)
                                                                                 ggTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGa                                                              >  2:1779938/1‑148 (MQ=255)
                                                                                    gtgACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGtt                                                           <  2:3323480/148‑1 (MQ=255)
                                                                                      gACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCa                                                         >  1:2467822/1‑148 (MQ=255)
                                                                                          taataaCCTGCATTGACTGCATCAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAt                                                     <  2:2775917/148‑1 (MQ=255)
                                                                                          taataaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCCAGTTCAAAAt                                                     >  2:718150/1‑148 (MQ=255)
                                                                                           aattaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCAGAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATc                                                    <  2:912490/148‑1 (MQ=255)
                                                                                            ataaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCt                                                   <  1:1918709/148‑1 (MQ=255)
                                                                                             taaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCaaaaaatt                                                  >  1:613752/1‑144 (MQ=255)
                                                                                              aaCCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTc                                                 <  1:1055685/148‑1 (MQ=255)
                                                                                                ccTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATATTCAt                                               >  1:1557772/1‑148 (MQ=255)
                                                                                                 cTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATa                                              >  2:143690/1‑148 (MQ=255)
                                                                                                   gCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAAc                                            >  1:807910/1‑148 (MQ=255)
                                                                                                    cATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTGATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACg                                           >  1:2561852/1‑148 (MQ=255)
                                                                                                     aTTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGa                                          <  2:2093200/148‑1 (MQ=255)
                                                                                                     aTTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGa                                          <  2:2816603/148‑1 (MQ=255)
                                                                                                       tGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATa                                        >  1:1899197/1‑148 (MQ=255)
                                                                                                       tGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATa                                        <  2:1108417/148‑1 (MQ=255)
                                                                                                       tGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATa                                        >  1:3282888/1‑148 (MQ=255)
                                                                                                        gACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATaa                                       <  1:1601108/148‑1 (MQ=255)
                                                                                                         aCTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAAc                                      >  2:1577869/1‑148 (MQ=255)
                                                                                                           tGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACtt                                    >  1:2168325/1‑148 (MQ=255)
                                                                                                            gCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACtta                                   >  1:1504548/1‑147 (MQ=255)
                                                                                                            gCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTc                                   <  2:2044766/148‑1 (MQ=255)
                                                                                                             cAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTcc                                  <  1:1429241/148‑1 (MQ=255)
                                                                                                               aCAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAg                                >  1:2279074/1‑148 (MQ=255)
                                                                                                                cAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGt                               >  2:491801/1‑148 (MQ=255)
                                                                                                                cAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGt                               <  2:1989336/148‑1 (MQ=255)
                                                                                                                      aGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGcc                         >  1:291189/1‑148 (MQ=255)
                                                                                                                       gTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                        <  2:2859855/148‑1 (MQ=255)
                                                                                                                       gTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCa                        <  2:645635/148‑1 (MQ=255)
                                                                                                                        tAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCaa                       >  2:1426293/1‑148 (MQ=255)
                                                                                                                         aaTTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAAt                      >  1:3504697/1‑148 (MQ=255)
                                                                                                                         aaTTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAAt                      >  2:1043336/1‑148 (MQ=255)
                                                                                                                          aTTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCaaag                     >  2:2672063/1‑146 (MQ=255)
                                                                                                                           ttttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTACAGTCAAGCCAATGt                    >  2:3111099/1‑148 (MQ=255)
                                                                                                                            tttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTa                   <  1:1998516/148‑1 (MQ=255)
                                                                                                                            tttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCCAGTTCAAAATATTCATAACGATAAATTACAGTCAAGCCCATGTa                   >  1:2670141/1‑148 (MQ=255)
                                                                                                                             ttGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAg                  <  1:2633120/148‑1 (MQ=255)
                                                                                                                                    ttGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCAttt           <  2:3423811/148‑1 (MQ=255)
                                                                                                                                       aCCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTaa        >  2:1525744/1‑148 (MQ=255)
                                                                                                                                       aCCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATATTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATGTTaa        >  2:607940/1‑148 (MQ=255)
                                                                                                                                        ccTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTaaa       <  2:3040871/148‑1 (MQ=255)
                                                                                                                                        ccTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTaaa       >  2:1953551/1‑148 (MQ=255)
                                                                                                                                          ttACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAAc     <  2:2624202/148‑1 (MQ=255)
                                                                                                                                           tACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACg    <  2:2015272/148‑1 (MQ=255)
                                                                                                                                             cTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACgtt  >  1:1082225/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTT  >  CP009273/580221‑580509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  CP009273/580217‑580515
                                                                                                                                                     |                                                                                                                                                     
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCT                                                                                                                                                       <  SRR19973065.1388581/150‑1 (MQ=60)
 TTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTA                                                                                                                                                      <  SRR19973065.2293411/150‑1 (MQ=60)
  TATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTAT                                                                                                                                                     >  SRR19973065.3444712/1‑150 (MQ=60)
  TATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTAT                                                                                                                                                     >  SRR19973065.220273/1‑150 (MQ=60)
     TATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAG                                                                                                                                                  <  SRR19973065.2283331/150‑1 (MQ=60)
          TGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATT                                                                                                                                             <  SRR19973065.536184/150‑1 (MQ=60)
           GATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTC                                                                                                                                            <  SRR19973065.2743265/150‑1 (MQ=60)
             TCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTT                                                                                                                                          <  SRR19973065.2040890/150‑1 (MQ=60)
              CATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTT                                                                                                                                         >  SRR19973065.483946/1‑150 (MQ=60)
               ATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTT                                                                                                                                        <  SRR19973065.832572/150‑1 (MQ=60)
               ATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTT                                                                                                                                        <  SRR19973065.68966/150‑1 (MQ=60)
                TAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTT                                                                                                                                       <  SRR19973065.2575542/150‑1 (MQ=60)
                TAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTT                                                                                                                                       <  SRR19973065.871870/150‑1 (MQ=60)
                  AAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCC                                                                                                                                     <  SRR19973065.541913/150‑1 (MQ=60)
                  AAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCC                                                                                                                                     <  SRR19973065.541841/150‑1 (MQ=60)
                  AAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCC                                                                                                                                     <  SRR19973065.2093910/150‑1 (MQ=60)
                     TGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGNCTTCAACATAAACTTTTGCGTTAGNTGNGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTNTGACCTTACTGCTATAAGTGATTCTTTTTCCCGG                                                                                                                                  >  SRR19973065.3422351/1‑150 (MQ=60)
                      GAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGG                                                                                                                                 <  SRR19973065.2877883/150‑1 (MQ=60)
                        AGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTC                                                                                                                               >  SRR19973065.1475384/1‑150 (MQ=60)
                        AGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTC                                                                                                                               >  SRR19973065.1475391/1‑150 (MQ=60)
                          TATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCAT                                                                                                                             >  SRR19973065.2640439/1‑150 (MQ=60)
                           ATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATA                                                                                                                            >  SRR19973065.1777003/1‑150 (MQ=60)
                            TTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAG                                                                                                                           <  SRR19973065.3214917/150‑1 (MQ=60)
                            TTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAG                                                                                                                           >  SRR19973065.797184/1‑150 (MQ=60)
                             TACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGT                                                                                                                          >  SRR19973065.3164262/1‑150 (MQ=60)
                                 TTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTC                                                                                                                      <  SRR19973065.2147375/150‑1 (MQ=60)
                                 TTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTC                                                                                                                      <  SRR19973065.2913362/150‑1 (MQ=60)
                                 TTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGGCATAGTGTTC                                                                                                                      <  SRR19973065.1413487/150‑1 (MQ=60)
                                   TTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCAT                                                                                                                    <  SRR19973065.111726/150‑1 (MQ=60)
                                    TTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATC                                                                                                                   <  SRR19973065.1973172/150‑1 (MQ=60)
                                    TTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATC                                                                                                                   <  SRR19973065.1972717/150‑1 (MQ=60)
                                       ATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTT                                                                                                                >  SRR19973065.3058383/1‑150 (MQ=60)
                                       ATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTT                                                                                                                >  SRR19973065.2080432/1‑150 (MQ=60)
                                       ATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTT                                                                                                                <  SRR19973065.2586070/150‑1 (MQ=60)
                                          CGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATC                                                                                                             >  SRR19973065.1975677/1‑150 (MQ=60)
                                            TAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAG                                                                                                           <  SRR19973065.1856209/150‑1 (MQ=60)
                                             AACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGA                                                                                                          >  SRR19973065.3194359/1‑150 (MQ=60)
                                              ACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGAT                                                                                                         >  SRR19973065.729256/1‑150 (MQ=60)
                                               CCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATG                                                                                                        <  SRR19973065.225305/150‑1 (MQ=60)
                                               CCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATG                                                                                                        <  SRR19973065.225164/150‑1 (MQ=60)
                                                CCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGA                                                                                                       >  SRR19973065.829532/1‑150 (MQ=60)
                                                CCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGA                                                                                                       >  SRR19973065.438399/1‑150 (MQ=60)
                                                   ATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973065.1257226/1‑150 (MQ=60)
                                                   ATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACATAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGATCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTC                                                                                                    >  SRR19973065.3016599/1‑150 (MQ=60)
                                                    TTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCC                                                                                                   <  SRR19973065.699812/150‑1 (MQ=60)
                                                      CCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCAC                                                                                                 >  SRR19973065.1521467/1‑150 (MQ=60)
                                                        ATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCC                                                                                               >  SRR19973065.65436/1‑150 (MQ=60)
                                                         TGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCA                                                                                              <  SRR19973065.860731/150‑1 (MQ=60)
                                                         TGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCA                                                                                              <  SRR19973065.860242/150‑1 (MQ=60)
                                                         TGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCA                                                                                              <  SRR19973065.1746114/150‑1 (MQ=60)
                                                         TGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCA                                                                                              <  SRR19973065.860389/150‑1 (MQ=60)
                                                         TGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCA                                                                                              >  SRR19973065.597687/1‑150 (MQ=60)
                                                            GCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCC                                                                                           >  SRR19973065.1185456/1‑150 (MQ=60)
                                                            GCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGGCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCC                                                                                           <  SRR19973065.1650196/150‑1 (MQ=60)
                                                              CTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGC                                                                                         >  SRR19973065.50711/1‑150 (MQ=60)
                                                              CTTCAACATAAACTTTAGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCACGGGTCATAGTGTTCATCGTTATCAGATCATTCCACCCAGCCGC                                                                                         >  SRR19973065.50728/1‑150 (MQ=60)
                                                                 CAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTTCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGT                                                                                      <  SRR19973065.451777/150‑1 (MQ=60)
                                                                 CAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGT                                                                                      >  SRR19973065.2453693/1‑150 (MQ=60)
                                                                   acacaaacactggcctgaggtccgacgaAATAACCTGCACTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAT                                                                                    <  SRR19973065.2968477/122‑1 (MQ=60)
                                                                   ACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTAT                                                                                    <  SRR19973065.1818103/150‑1 (MQ=60)
                                                                    CATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATT                                                                                   <  SRR19973065.2543329/150‑1 (MQ=60)
                                                                     ATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTT                                                                                  <  SRR19973065.958513/150‑1 (MQ=60)
                                                                     ATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTT                                                                                  >  SRR19973065.2636047/1‑150 (MQ=60)
                                                                      TAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTA                                                                                 >  SRR19973065.3532489/1‑150 (MQ=60)
                                                                      TAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTA                                                                                 >  SRR19973065.1787399/1‑150 (MQ=60)
                                                                      TAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTA                                                                                 >  SRR19973065.1787417/1‑150 (MQ=60)
                                                                         ACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAAT                                                                              <  SRR19973065.2259202/150‑1 (MQ=60)
                                                                         ACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAAT                                                                              <  SRR19973065.187075/150‑1 (MQ=60)
                                                                          CTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATG                                                                             <  SRR19973065.1369275/150‑1 (MQ=60)
                                                                             TTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGC                                                                          >  SRR19973065.3059487/1‑150 (MQ=60)
                                                                              TGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCC                                                                         <  SRR19973065.517287/150‑1 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973065.2110154/1‑150 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973065.1297542/1‑150 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       <  SRR19973065.64345/150‑1 (MQ=60)
                                                                                CGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCAC                                                                       >  SRR19973065.3266930/1‑150 (MQ=60)
                                                                                 GTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACC                                                                      >  SRR19973065.1538778/1‑150 (MQ=60)
                                                                                    AGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAG                                                                   <  SRR19973065.799090/150‑1 (MQ=60)
                                                                                        GTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCA                                                               >  SRR19973065.2483371/1‑150 (MQ=60)
                                                                                          GACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCAGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCAGCTGTATTTAAAATTGCCACCGAGTTCAAA                                                             >  SRR19973065.1485506/1‑150 (MQ=60)
                                                                                            CGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAT                                                           <  SRR19973065.1527530/150‑1 (MQ=60)
                                                                                               AATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTTCCACCGAGTTCAAAATCTT                                                        >  SRR19973065.617675/1‑150 (MQ=60)
                                                                                               AATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAAaatt                                                        >  SRR19973065.617677/1‑146 (MQ=60)
                                                                                               AATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCGGTATTTAAATGTGGCACCGAGTTCAAAATCTT                                                        >  SRR19973065.617657/1‑150 (MQ=60)
                                                                                                ATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTC                                                       <  SRR19973065.1931294/150‑1 (MQ=60)
                                                                                                  AACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTGCCACCGAGTTCAAAATCTTCAT                                                     >  SRR19973065.1568140/1‑150 (MQ=60)
                                                                                                  AACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCAT                                                     <  SRR19973065.1062687/150‑1 (MQ=60)
                                                                                                  AACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATATTCAT                                                     >  SRR19973065.1568109/1‑150 (MQ=60)
                                                                                                   ACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATA                                                    <  SRR19973065.1908284/150‑1 (MQ=60)
                                                                                                    CCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTTCCACCCAGTTCAAAAACTTCAAAA                                                   >  SRR19973065.55475/1‑150 (MQ=60)
                                                                                                    CCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAA                                                   >  SRR19973065.1842606/1‑150 (MQ=60)
                                                                                                     CTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAAC                                                  >  SRR19973065.813244/1‑150 (MQ=60)
                                                                                                      TGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTGATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGGATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACG                                                 >  SRR19973065.2577867/1‑150 (MQ=60)
                                                                                                      TGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACG                                                 <  SRR19973065.1802989/150‑1 (MQ=60)
                                                                                                         ATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTGCCACCGAGTTCAAAATCTTCATAACGATA                                              >  SRR19973065.1911710/1‑150 (MQ=60)
                                                                                                         ATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATA                                              >  SRR19973065.1911677/1‑150 (MQ=60)
                                                                                                         ATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATA                                              >  SRR19973065.3302847/1‑150 (MQ=60)
                                                                                                         ATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATA                                              >  SRR19973065.3420553/1‑150 (MQ=60)
                                                                                                            GACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACT                                           <  SRR19973065.1611809/150‑1 (MQ=60)
                                                                                                            GACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACT                                           <  SRR19973065.2396845/150‑1 (MQ=60)
                                                                                                             ACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTT                                          >  SRR19973065.2182251/1‑150 (MQ=60)
                                                                                                             ACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTT                                          >  SRR19973065.2182249/1‑150 (MQ=60)
                                                                                                              CTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTA                                         >  SRR19973065.1514303/1‑150 (MQ=60)
                                                                                                                 CAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAG                                      >  SRR19973065.2293450/1‑150 (MQ=60)
                                                                                                                 CAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAG                                      <  SRR19973065.1438676/150‑1 (MQ=60)
                                                                                                                 CAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAG                                      <  SRR19973065.1438540/150‑1 (MQ=60)
                                                                                                                 CAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAG                                      >  SRR19973065.514920/1‑150 (MQ=60)
                                                                                                                  AACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATCAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTTAATGTGCCACCCAGTTCAAAATCTTCAAAACTATAACTTCCAGT                                     >  SRR19973065.1520659/1‑150 (MQ=60)
                                                                                                                  AACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGT                                     >  SRR19973065.1480683/1‑150 (MQ=60)
                                                                                                                  AACAGAATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGT                                     >  SRR19973065.1355730/1‑150 (MQ=60)
                                                                                                                        ATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCC                               >  SRR19973065.293081/1‑150 (MQ=60)
                                                                                                                        ATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACGTCCAGTCAAGCC                               >  SRR19973065.1962188/1‑150 (MQ=60)
                                                                                                                        ATAGTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACGTCCAGTCAAGCC                               >  SRR19973065.177158/1‑150 (MQ=60)
                                                                                                                           GTAATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAAT                            >  SRR19973065.3525977/1‑150 (MQ=60)
                                                                                                                              ATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCCAGTTCAAAATATTCATAACGATAAATTACAGTCAAGCCCATGTA                         >  SRR19973065.2686680/1‑150 (MQ=60)
                                                                                                                              ATTTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCGGTATATAAATTTGCCACCCAGTTCAAAATCTTAAAAAAGAAAAATTCCAGTCAAGCCAAatta                         >  SRR19973065.2685797/1‑146 (MQ=60)
                                                                                                                                TTTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGG                       <  SRR19973065.2011457/150‑1 (MQ=60)
                                                                                                                                 TTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGG                      <  SRR19973065.2649393/150‑1 (MQ=60)
                                                                                                                                 TTGGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGG                      <  SRR19973065.1642532/150‑1 (MQ=60)
                                                                                                                                   GGTCTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCA                    <  SRR19973065.3401390/150‑1 (MQ=60)
                                                                                                                                      CTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTT                 >  SRR19973065.2606351/1‑150 (MQ=60)
                                                                                                                                      CTTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAAATTCCAGTCAAGCCAATGTAGGGCATTT                 >  SRR19973065.802118/1‑150 (MQ=60)
                                                                                                                                       TTTGACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATATTCATAACGATAACTTACAGTCAAGCCAATGTAGGGCATTTT                >  SRR19973065.1001405/1‑150 (MQ=60)
                                                                                                                                           ACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTTCCACCGAGTTCAAAATCTTCATAACGATAACTTACAGTCAAGCCAATGTAGGGCATTTTAAAA            >  SRR19973065.1236755/1‑150 (MQ=60)
                                                                                                                                           ACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTTCCACCGAGTTCAAAATCTTCATAACGATAAATTCCAGTCAATCCAATGTAGGGCAATTTAAAA            >  SRR19973065.424337/1‑150 (MQ=60)
                                                                                                                                           ACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAA            <  SRR19973065.2840259/150‑1 (MQ=60)
                                                                                                                                           ACCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAA            <  SRR19973065.1077377/150‑1 (MQ=60)
                                                                                                                                            CCTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAAC           <  SRR19973065.2973846/150‑1 (MQ=60)
                                                                                                                                             CTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATTTGCCACCCAGTTCAAAATATTCATAACGAAAACATCCACTCAAGCCAATCTAGGCCAATTTAAAAat          >  SRR19973065.2245023/1‑148 (MQ=60)
                                                                                                                                             CTTACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATATTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACG          >  SRR19973065.2498524/1‑150 (MQ=60)
                                                                                                                                               TACTGCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTT        >  SRR19973065.1089369/1‑150 (MQ=60)
                                                                                                                                                   GCTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTAAAAATATTCATAACGATAACTTCCAGTCAAGCCAAAGTAGGGCATTTTAAAACGTTGTTT    >  SRR19973065.207553/1‑150 (MQ=60)
                                                                                                                                                    CTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG   <  SRR19973065.3236899/150‑1 (MQ=60)
                                                                                                                                                    CTATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTG   >  SRR19973065.581716/1‑150 (MQ=60)
                                                                                                                                                     TATAAGTGATTCTTTTTCCCTGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCTCTGTATTTAAATGTGCCACCTAGTTAAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  <  SRR19973065.440783/150‑1 (MQ=60)
                                                                                                                                                     TATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  SRR19973065.1835301/1‑150 (MQ=60)
                                                                                                                                                     TATAAGTGATTCTTTTTCCCGGGTCAGAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  <  SRR19973065.902803/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTATTATTGTGATCATAAAGTGAAGTATTACCTTTTTTATTCGTAACCCGATTCCATGCGCCTTCAACATAAACTTTTGCGTTAGGTGTGACGTAATAACCTGCATTGACTGCAACAGAATAGTAATTTTGGTCTTTGACCTTACTGCGATAAGTGATTCTTTTTCCCGGGTCATAGTGTTCATCGTTATCAGATGATTCCACCCAGCCGCTGTATTTAAATGTGCCACCGAGTTCAAAATCTTCATAACGATAACTTCCAGTCAAGCCAATGTAGGGCATTTTAAAACGTTGTTTGT  >  CP009273/580217‑580515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑