Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,940,498 G→T 87.9% intergenic (‑61/‑10) yobI ← / → yebB uncharacterized protein/DUF830 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,940,4980GT87.9% 651.6 / 92.8 265intergenic (‑61/‑10)yobI/yebBuncharacterized protein/DUF830 family protein
Reads supporting (aligned to +/- strand):  ref base G (14/18);  new base T (109/124);  total (123/142)
Fisher's exact test for biased strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 268 total aligned reads displayed.

ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGT  >  CP009273/1940350‑1940645
                                                                                                                                                    |                                                                                                                                                   
acaGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAGAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATgg                                                                                                                                                     <  1:3006832/149‑1 (MQ=255)
   gTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAAt                                                                                                                                                  >  2:868024/1‑149 (MQ=255)
   gTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAAt                                                                                                                                                  <  2:3621091/149‑1 (MQ=255)
    tataATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATg                                                                                                                                                 >  1:1227466/1‑149 (MQ=255)
    tataATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATg                                                                                                                                                 <  1:875074/149‑1 (MQ=255)
    tataATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATg                                                                                                                                                 >  1:2478498/1‑149 (MQ=255)
       aaTTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACa                                                                                                                                              >  2:2417497/1‑149 (MQ=255)
        aTTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACaa                                                                                                                                             <  1:2813137/149‑1 (MQ=255)
         tttATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAc                                                                                                                                            >  2:3720734/1‑149 (MQ=255)
         tttATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAc                                                                                                                                            >  1:3177814/1‑149 (MQ=255)
            aTTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTg                                                                                                                                         <  2:4326023/149‑1 (MQ=255)
              tCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGaa                                                                                                                                       >  1:1648169/1‑149 (MQ=255)
                 aCAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAatat                                                                                                                                    <  1:1177960/149‑1 (MQ=255)
                  cAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATatt                                                                                                                                   <  2:1553558/149‑1 (MQ=255)
                  cAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATatt                                                                                                                                   <  2:1281314/149‑1 (MQ=255)
                       tataTTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATattaatta                                                                                                                              <  1:1131079/149‑1 (MQ=255)
                          atTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTAccc                                                                                                                           <  2:4737430/149‑1 (MQ=255)
                          atTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTAccc                                                                                                                           <  2:1937132/149‑1 (MQ=255)
                          atTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTAccc                                                                                                                           >  1:3233533/1‑149 (MQ=255)
                            tgtaaGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCctg                                                                                                                         <  2:1073692/146‑1 (MQ=255)
                            tGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCctg                                                                                                                         >  2:3927529/1‑149 (MQ=255)
                                    aaTATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAatat                                                                                                                 <  1:1756200/149‑1 (MQ=255)
                                    aaTATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAatat                                                                                                                 >  1:1392913/1‑149 (MQ=255)
                                    aaTATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAatat                                                                                                                 <  2:3493141/149‑1 (MQ=255)
                                     atatatTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGACTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATg                                                                                                                <  2:3974896/149‑1 (MQ=255)
                                      tatTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGa                                                                                                               <  2:1230692/149‑1 (MQ=255)
                                       atTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGaa                                                                                                              >  1:3601121/1‑149 (MQ=255)
                                       atTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGaa                                                                                                              <  1:1479375/149‑1 (MQ=255)
                                           tAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTg                                                                                                          <  2:3951533/149‑1 (MQ=255)
                                             atatTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGga                                                                                                        <  1:1979075/149‑1 (MQ=255)
                                               atTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGgaga                                                                                                      >  2:1136448/1‑149 (MQ=255)
                                                ttACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat                                                                                                     <  2:701819/149‑1 (MQ=255)
                                                ttACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat                                                                                                     <  1:4543075/149‑1 (MQ=255)
                                                  aCATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATACGAAATTGGAGatat                                                                                                   <  2:2767209/149‑1 (MQ=255)
                                                  aCATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATACGAAATTGGAGatat                                                                                                   <  2:374142/149‑1 (MQ=255)
                                                   cATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATc                                                                                                  >  1:4031905/1‑149 (MQ=255)
                                                     tatTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGt                                                                                                <  1:1641278/149‑1 (MQ=255)
                                                      atTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAATAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTc                                                                                               <  1:3932297/149‑1 (MQ=255)
                                                      atTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTc                                                                                               <  1:3875926/149‑1 (MQ=255)
                                                       ttCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCt                                                                                              >  1:4577263/1‑149 (MQ=255)
                                                       ttCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCt                                                                                              <  1:2157673/149‑1 (MQ=255)
                                                        tCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt                                                                                             >  2:1747450/1‑149 (MQ=255)
                                                        tCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt                                                                                             <  1:4286211/149‑1 (MQ=255)
                                                         cAGTGAAGAAATGCGTAATAACAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCttt                                                                                            <  2:2144594/149‑1 (MQ=255)
                                                          aGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTa                                                                                           <  1:2085629/149‑1 (MQ=255)
                                                           gTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACACTGCTGAATATGAAATTGGAGATATCGTCTTTAc                                                                                          >  2:2234686/1‑149 (MQ=255)
                                                             gaagaaATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTTCGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACAt                                                                                        >  2:1428633/1‑149 (MQ=255)
                                                             gaagaaATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACAt                                                                                        <  1:2926972/149‑1 (MQ=255)
                                                                  aaTGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGtata                                                                                   <  1:1797535/149‑1 (MQ=255)
                                                                  aaTGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGtata                                                                                   <  2:3641279/149‑1 (MQ=255)
                                                                       gTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGtgc                                                                              <  2:4297306/149‑1 (MQ=255)
                                                                       gTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGtgc                                                                              >  2:4067268/1‑149 (MQ=255)
                                                                       gTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGtgc                                                                              <  2:1907785/149‑1 (MQ=255)
                                                                            aaaaaTATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCt                                                                         >  2:2858445/1‑149 (MQ=255)
                                                                               aaTATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTAt                                                                      <  1:1868366/149‑1 (MQ=255)
                                                                                   taCAGTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTgg                                                                  <  1:415376/149‑1 (MQ=255)
                                                                                     cATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTc                                                                <  1:577777/149‑1 (MQ=255)
                                                                                      aTTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCa                                                               >  1:1602555/1‑149 (MQ=255)
                                                                                         gcgcCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAat                                                            >  2:3701800/1‑149 (MQ=255)
                                                                                         gcgcCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAat                                                            >  1:1019298/1‑149 (MQ=255)
                                                                                            cctcctGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATc                                                         >  2:3820495/1‑149 (MQ=255)
                                                                                             ctcctGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCa                                                        >  1:3016006/1‑149 (MQ=255)
                                                                                               cctGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGc                                                      <  1:4069325/149‑1 (MQ=255)
                                                                                                 tGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTg                                                    >  2:3530017/1‑149 (MQ=255)
                                                                                                   aaaaaaTAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCa                                                  >  1:2196590/1‑149 (MQ=255)
                                                                                                   aaaaaaTAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCa                                                  >  1:1500101/1‑149 (MQ=255)
                                                                                                     aaaaTAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATc                                                >  2:3118710/1‑149 (MQ=255)
                                                                                                       aataaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCaa                                              <  2:2634691/149‑1 (MQ=255)
                                                                                                        ataaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCaaa                                             >  1:2447885/1‑149 (MQ=255)
                                                                                                        ataaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCaaa                                             >  2:1852324/1‑149 (MQ=255)
                                                                                                        ataaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCaaa                                             >  1:4735646/1‑149 (MQ=255)
                                                                                                         taaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAAt                                            >  1:3419302/1‑149 (MQ=255)
                                                                                                          aaatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAAtt                                           <  2:1333658/149‑1 (MQ=255)
                                                                                                           aatTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTg                                          <  2:4458799/149‑1 (MQ=255)
                                                                                                                    tGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTaa                                 <  2:2741454/149‑1 (MQ=255)
                                                                                                                     gCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAAt                                >  2:4688570/1‑149 (MQ=255)
                                                                                                                         ttACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACg                            <  2:304252/149‑1 (MQ=255)
                                                                                                                           aCGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTc                          >  2:2922687/1‑149 (MQ=255)
                                                                                                                           aCGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTc                          >  1:1803027/1‑149 (MQ=255)
                                                                                                                             gTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCgg                        >  1:262625/1‑149 (MQ=255)
                                                                                                                               atatTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGa                      >  2:3333395/1‑149 (MQ=255)
                                                                                                                               atatTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGa                      >  1:3487842/1‑149 (MQ=255)
                                                                                                                                tatTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGAt                     <  2:2268431/149‑1 (MQ=255)
                                                                                                                                 atTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATc                    <  2:4190614/149‑1 (MQ=255)
                                                                                                                                 atTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATc                    <  1:1671567/149‑1 (MQ=255)
                                                                                                                                  tttGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCa                   >  1:1906937/1‑149 (MQ=255)
                                                                                                                                  tttGTATCAATTTCAATGTAATGACAACGGGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCa                   <  1:3812347/149‑1 (MQ=255)
                                                                                                                                   ttGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCAt                  <  1:4609600/149‑1 (MQ=255)
                                                                                                                                      tATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTAt               >  2:2543066/1‑149 (MQ=255)
                                                                                                                                      tATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTAt               >  2:1516951/1‑149 (MQ=255)
                                                                                                                                      tATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTAt               >  2:1032464/1‑149 (MQ=255)
                                                                                                                                       aTCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATc              >  2:3193503/1‑149 (MQ=255)
                                                                                                                                          tATTTCAATGTAATGACAACGTGAATATTAATTACCCGGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGt           <  2:339798/149‑1 (MQ=255)
                                                                                                                                            tttCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCa         >  1:653581/1‑149 (MQ=255)
                                                                                                                                            tttCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCa         <  1:2417497/149‑1 (MQ=255)
                                                                                                                                            tttCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCa         >  2:4755068/1‑149 (MQ=255)
                                                                                                                                            tttCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCa         <  1:3700683/149‑1 (MQ=255)
                                                                                                                                              tCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATa       <  1:4722196/149‑1 (MQ=255)
                                                                                                                                               cAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATaa      <  2:1793302/149‑1 (MQ=255)
                                                                                                                                                   ggAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGt  <  1:594759/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGT  >  CP009273/1940350‑1940645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  >  CP009273/1940350‑1940646
                                                                                                                                                    |                                                                                                                                                    
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAGAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGA                                                                                                                                                     <  SRR19973071.3017568/150‑1 (MQ=60)
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTA                                                                                                                                                     <  SRR19973071.3763884/150‑1 (MQ=60)
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTA                                                                                                                                                     <  SRR19973071.3763889/150‑1 (MQ=60)
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTA                                                                                                                                                     <  SRR19973071.3548659/150‑1 (MQ=60)
   GTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATG                                                                                                                                                  >  SRR19973071.2487515/1‑150 (MQ=60)
   GTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATG                                                                                                                                                  >  SRR19973071.1231778/1‑150 (MQ=60)
    TATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGA                                                                                                                                                 <  SRR19973071.1618893/150‑1 (MQ=60)
    TATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGA                                                                                                                                                 <  SRR19973071.878203/150‑1 (MQ=60)
        ATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAC                                                                                                                                             >  SRR19973071.3188886/1‑150 (MQ=60)
        ATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAC                                                                                                                                             <  SRR19973071.2823073/150‑1 (MQ=60)
        ATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAC                                                                                                                                             <  SRR19973071.2176475/150‑1 (MQ=60)
        ATTTAGTCTACATGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAACAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAAC                                                                                                                                             <  SRR19973071.2823079/150‑1 (MQ=60)
           TATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTG                                                                                                                                          <  SRR19973071.1654187/150‑1 (MQ=60)
           TAGTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTG                                                                                                                                          <  SRR19973071.1654138/150‑1 (MQ=60)
            ATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGA                                                                                                                                         >  SRR19973071.3586525/1‑150 (MQ=60)
             TTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAA                                                                                                                                        >  SRR19973071.1654173/1‑150 (MQ=60)
             TTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAA                                                                                                                                        >  SRR19973071.1654137/1‑150 (MQ=60)
               CTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATA                                                                                                                                      >  SRR19973071.2049971/1‑150 (MQ=60)
                 ACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATT                                                                                                                                    <  SRR19973071.1182016/150‑1 (MQ=60)
                    GGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATTACAACGTGAATATTAAT                                                                                                                                 >  SRR19973071.681104/1‑150 (MQ=60)
                    GGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAAT                                                                                                                                 >  SRR19973071.1468874/1‑150 (MQ=60)
                     GTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATT                                                                                                                                >  SRR19973071.1799985/1‑150 (MQ=60)
                       TATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTAC                                                                                                                              <  SRR19973071.1374212/150‑1 (MQ=60)
                       TATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTAC                                                                                                                              <  SRR19973071.1135036/150‑1 (MQ=60)
                         TATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCC                                                                                                                            >  SRR19973071.3244910/1‑150 (MQ=60)
                           TTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTG                                                                                                                          >  SRR19973071.2546916/1‑150 (MQ=60)
                             GGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCT                                                                                                                        >  SRR19973071.143825/1‑150 (MQ=60)
                             GGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCT                                                                                                                        >  SRR19973071.1687656/1‑150 (MQ=60)
                             GGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCT                                                                                                                        <  SRR19973071.4707317/150‑1 (MQ=60)
                             GGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCT                                                                                                                        <  SRR19973071.3791451/150‑1 (MQ=60)
                              GAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTG                                                                                                                       <  SRR19973071.4116865/150‑1 (MQ=60)
                              GAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTG                                                                                                                       <  SRR19973071.1719559/150‑1 (MQ=60)
                               AAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGA                                                                                                                      >  SRR19973071.4590970/1‑150 (MQ=60)
                                 GCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAAT                                                                                                                    <  SRR19973071.2453504/150‑1 (MQ=60)
                                   AAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATAT                                                                                                                  >  SRR19973071.1398048/1‑150 (MQ=60)
                                    AATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATG                                                                                                                 <  SRR19973071.1762756/150‑1 (MQ=60)
                                      TATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAA                                                                                                               >  SRR19973071.3613663/1‑150 (MQ=60)
                                       ATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAA                                                                                                              <  SRR19973071.1484758/150‑1 (MQ=60)
                                       ATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAA                                                                                                              <  SRR19973071.1485030/150‑1 (MQ=60)
                                        TTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAAT                                                                                                             >  SRR19973071.3191497/1‑150 (MQ=60)
                                         TTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATT                                                                                                            <  SRR19973071.210667/150‑1 (MQ=60)
                                          TTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTG                                                                                                           <  SRR19973071.2721122/150‑1 (MQ=60)
                                             ATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAG                                                                                                        <  SRR19973071.4155981/150‑1 (MQ=60)
                                             ATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAG                                                                                                        <  SRR19973071.1986238/150‑1 (MQ=60)
                                               ATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAGAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGAT                                                                                                      <  SRR19973071.1493812/150‑1 (MQ=60)
                                                TTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATA                                                                                                     <  SRR19973071.4559259/150‑1 (MQ=60)
                                                TTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATA                                                                                                     <  SRR19973071.4559281/150‑1 (MQ=60)
                                                  ACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATC                                                                                                   >  SRR19973071.905140/1‑150 (MQ=60)
                                                  ACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATC                                                                                                   <  SRR19973071.3043335/150‑1 (MQ=60)
                                                  ACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATC                                                                                                   <  SRR19973071.3043383/150‑1 (MQ=60)
                                                  ACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATC                                                                                                   >  SRR19973071.4046110/1‑150 (MQ=60)
                                                   CATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCG                                                                                                  >  SRR19973071.1914786/1‑150 (MQ=60)
                                                   CATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCG                                                                                                  >  SRR19973071.4312515/1‑150 (MQ=60)
                                                     TATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTC                                                                                                <  SRR19973071.103995/150‑1 (MQ=60)
                                                     TATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTC                                                                                                <  SRR19973071.1647243/150‑1 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAATAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               <  SRR19973071.3946140/150‑1 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               <  SRR19973071.3890426/150‑1 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               >  SRR19973071.4593581/1‑150 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               <  SRR19973071.3889633/150‑1 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               <  SRR19973071.2647347/150‑1 (MQ=60)
                                                      ATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCT                                                                                               <  SRR19973071.3889611/150‑1 (MQ=60)
                                                       TTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTT                                                                                              <  SRR19973071.2165648/150‑1 (MQ=60)
                                                        TCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTT                                                                                             <  SRR19973071.4301381/150‑1 (MQ=60)
                                                        TCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTT                                                                                             <  SRR19973071.4301185/150‑1 (MQ=60)
                                                        TCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTT                                                                                             <  SRR19973071.3489071/150‑1 (MQ=60)
                                                        TCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTT                                                                                             <  SRR19973071.804032/150‑1 (MQ=60)
                                                         CAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTA                                                                                            >  SRR19973071.1505590/1‑150 (MQ=60)
                                                          AGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTAC                                                                                           <  SRR19973071.2093314/150‑1 (MQ=60)
                                                           GTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACA                                                                                          >  SRR19973071.1878005/1‑150 (MQ=60)
                                                            TGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACAT                                                                                         <  SRR19973071.4051351/150‑1 (MQ=60)
                                                             GAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATG                                                                                        <  SRR19973071.4740797/150‑1 (MQ=60)
                                                             GAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATG                                                                                        <  SRR19973071.2937461/150‑1 (MQ=60)
                                                             GAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATG                                                                                        <  SRR19973071.2937497/150‑1 (MQ=60)
                                                               AGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTA                                                                                      <  SRR19973071.599213/150‑1 (MQ=60)
                                                                caaatccgaacaaacaatataccttgtcccacctgcAAAAATAAATTTTTTATGCTATTACGTATATTTGGATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTAT                                                                                     <  SRR19973071.2615492/114‑1 (MQ=60)
                                                                 AAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATA                                                                                    <  SRR19973071.2598775/150‑1 (MQ=60)
                                                                  AATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAG                                                                                   <  SRR19973071.1804229/150‑1 (MQ=60)
                                                                  AATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAG                                                                                   >  SRR19973071.3927087/1‑150 (MQ=60)
                                                                       GTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCT                                                                              <  SRR19973071.1641149/150‑1 (MQ=60)
                                                                       GTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCT                                                                              <  SRR19973071.2911640/150‑1 (MQ=60)
                                                                       GTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCT                                                                              >  SRR19973071.2457404/1‑150 (MQ=60)
                                                                               AATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATT                                                                      <  SRR19973071.1875443/150‑1 (MQ=60)
                                                                               AATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATT                                                                      <  SRR19973071.1875258/150‑1 (MQ=60)
                                                                                   TACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGT                                                                  <  SRR19973071.417325/150‑1 (MQ=60)
                                                                                   TACAGTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGT                                                                  <  SRR19973071.416751/150‑1 (MQ=60)
                                                                                     CATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCA                                                                >  SRR19973071.1608198/1‑150 (MQ=60)
                                                                                     CATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCA                                                                <  SRR19973071.2053912/150‑1 (MQ=60)
                                                                                     CATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCA                                                                <  SRR19973071.579943/150‑1 (MQ=60)
                                                                                     CATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTAGATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGACTATGAACTTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCA                                                                >  SRR19973071.3432104/1‑150 (MQ=60)
                                                                                      ATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAA                                                               >  SRR19973071.1153634/1‑150 (MQ=60)
                                                                                        TGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAAT                                                             >  SRR19973071.1022994/1‑150 (MQ=60)
                                                                                          CGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATAT                                                           >  SRR19973071.580258/1‑150 (MQ=60)
                                                                                            CCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCA                                                         >  SRR19973071.3026764/1‑150 (MQ=60)
                                                                                            CCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCA                                                         >  SRR19973071.3026489/1‑150 (MQ=60)
                                                                                              TCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGC                                                       >  SRR19973071.4780594/1‑150 (MQ=60)
                                                                                              TCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGC                                                       >  SRR19973071.1218896/1‑150 (MQ=60)
                                                                                               CCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCT                                                      <  SRR19973071.4083732/150‑1 (MQ=60)
                                                                                                  GAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCA                                                   >  SRR19973071.2204710/1‑150 (MQ=60)
                                                                                                  GAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCA                                                   >  SRR19973071.1505527/1‑150 (MQ=60)
                                                                                                       AATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAA                                              >  SRR19973071.2455783/1‑150 (MQ=60)
                                                                                                       AATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAA                                              >  SRR19973071.2456780/1‑150 (MQ=60)
                                                                                                       AATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAA                                              >  SRR19973071.2129865/1‑150 (MQ=60)
                                                                                                       AATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAA                                              >  SRR19973071.4752406/1‑150 (MQ=60)
                                                                                                        ATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAAT                                             >  SRR19973071.3431407/1‑150 (MQ=60)
                                                                                                          AAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTG                                           <  SRR19973071.183284/150‑1 (MQ=60)
                                                                                                           AATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGC                                          >  SRR19973071.3017885/1‑150 (MQ=60)
                                                                                                           AATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGC                                          >  SRR19973071.3355101/1‑150 (MQ=60)
                                                                                                                   ATGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAA                                  >  SRR19973071.4744121/1‑150 (MQ=60)
                                                                                                                    TGCTATTACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAAT                                 >  SRR19973071.2274500/1‑150 (MQ=60)
                                                                                                                          TACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTC                           >  SRR19973071.1809531/1‑150 (MQ=60)
                                                                                                                          TACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTC                           >  SRR19973071.1809740/1‑150 (MQ=60)
                                                                                                                          TACGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTC                           >  SRR19973071.1809752/1‑150 (MQ=60)
                                                                                                                            CGTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGG                         >  SRR19973071.263604/1‑150 (MQ=60)
                                                                                                                             GTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGG                        >  SRR19973071.1176156/1‑150 (MQ=60)
                                                                                                                             GTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGG                        >  SRR19973071.4608609/1‑150 (MQ=60)
                                                                                                                             GTATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGG                        >  SRR19973071.4608659/1‑150 (MQ=60)
                                                                                                                             GTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGG                        <  SRR19973071.330272/150‑1 (MQ=60)
                                                                                                                              TATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGA                       <  SRR19973071.1208104/150‑1 (MQ=60)
                                                                                                                              TATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGA                       >  SRR19973071.3500111/1‑150 (MQ=60)
                                                                                                                               ATATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGAT                      >  SRR19973071.180278/1‑150 (MQ=60)
                                                                                                                                 ATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCA                    >  SRR19973071.1913933/1‑150 (MQ=60)
                                                                                                                                 ATTTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCA                    <  SRR19973071.1677711/150‑1 (MQ=60)
                                                                                                                                  TTTGTATCAATTTCAATGTAATGACAACGGGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCAT                   <  SRR19973071.3825876/150‑1 (MQ=60)
                                                                                                                                   TTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATT                  <  SRR19973071.2220840/150‑1 (MQ=60)
                                                                                                                                   TTGTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATT                  <  SRR19973071.4626039/150‑1 (MQ=60)
                                                                                                                                     GTATCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTAT                <  SRR19973071.3667097/150‑1 (MQ=60)
                                                                                                                                       ATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCG              <  SRR19973071.998307/150‑1 (MQ=60)
                                                                                                                                        TCTATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGG             <  SRR19973071.3802623/150‑1 (MQ=60)
                                                                                                                                          TATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTC           >  SRR19973071.50285/1‑150 (MQ=60)
                                                                                                                                          TATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTC           <  SRR19973071.36302/150‑1 (MQ=60)
                                                                                                                                           ATTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCA          >  SRR19973071.655966/1‑150 (MQ=60)
                                                                                                                                            TTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCAT         <  SRR19973071.3713789/150‑1 (MQ=60)
                                                                                                                                            TTTCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCAT         <  SRR19973071.2426228/150‑1 (MQ=60)
                                                                                                                                              TCAATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAA       <  SRR19973071.4738933/150‑1 (MQ=60)
                                                                                                                                                AATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACG     >  SRR19973071.4035138/1‑150 (MQ=60)
                                                                                                                                                AATGTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACG     >  SRR19973071.4035130/1‑150 (MQ=60)
                                                                                                                                                   GTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  >  SRR19973071.438727/1‑150 (MQ=60)
                                                                                                                                                   GTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  >  SRR19973071.438666/1‑150 (MQ=60)
                                                                                                                                                   GTAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  <  SRR19973071.871130/150‑1 (MQ=60)
                                                                                                                                                   GGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  <  SRR19973071.596975/150‑1 (MQ=60)
                                                                                                                                                   GGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  <  SRR19973071.597113/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
ACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTTTACATGTATAGGTGCTGCCTTATTTGGTCAAATATCAGCTGCATCAAATTGCTGGAGTAATCACGTCGGGATCATTATCGGTCATAACGGTG  >  CP009273/1940350‑1940646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑