Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,485 C→T 17.6% intergenic (‑302/‑222) hemB ← / → yaiT 5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,4850CT17.6% 516.0 / 90.8 199intergenic (‑302/‑222)hemB/yaiT5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base C (84/79);  new base T (14/21);  total (99/100)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 200 total aligned reads displayed.

GAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCG  >  CP009273/385336‑385633
                                                                                                                                                     |                                                                                                                                                    
   aaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaa                                                                                                                                                   <  2:2391649/150‑1 (MQ=255)
    atatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaa                                                                                                                                                  <  2:4394071/150‑1 (MQ=255)
     tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaaa                                                                                                                                                 >  1:360442/1‑150 (MQ=255)
     tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaaa                                                                                                                                                 >  1:301781/1‑150 (MQ=255)
      atTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAAc                                                                                                                                                <  2:3014852/150‑1 (MQ=255)
      atTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAAc                                                                                                                                                <  2:1245458/150‑1 (MQ=255)
      atTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAAc                                                                                                                                                <  1:997815/150‑1 (MQ=255)
       tttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa                                                                                                                                               <  1:965534/150‑1 (MQ=255)
        ttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaa                                                                                                                                              <  1:678403/150‑1 (MQ=255)
          ttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACaaaa                                                                                                                                            >  1:2760344/1‑150 (MQ=255)
          ttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaaa                                                                                                                                            <  2:3782677/150‑1 (MQ=255)
             aTCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTa                                                                                                                                         >  2:2587415/1‑150 (MQ=255)
              tCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTAt                                                                                                                                        <  1:1851050/150‑1 (MQ=255)
                  tatttatAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGc                                                                                                                                    >  1:3629607/1‑150 (MQ=255)
                   atttatAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCa                                                                                                                                   >  1:2042173/1‑150 (MQ=255)
                   atttatAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCa                                                                                                                                   >  2:2820450/1‑150 (MQ=255)
                   atttatAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCa                                                                                                                                   <  2:2737432/150‑1 (MQ=255)
                     ttatAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAAc                                                                                                                                 >  2:1703253/1‑150 (MQ=255)
                      tataGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACa                                                                                                                                <  2:2578488/150‑1 (MQ=255)
                       ataGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACAt                                                                                                                               >  1:1873691/1‑150 (MQ=255)
                       ataGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACAt                                                                                                                               >  2:3325722/1‑150 (MQ=255)
                         aGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGt                                                                                                                             >  1:4214750/1‑150 (MQ=255)
                         aGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGt                                                                                                                             <  2:1793581/150‑1 (MQ=255)
                         aGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGATTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGt                                                                                                                             <  2:4628125/150‑1 (MQ=255)
                            tGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGa                                                                                                                          >  1:2459360/1‑150 (MQ=255)
                              ttGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGaaa                                                                                                                        <  1:219636/150‑1 (MQ=255)
                                ggTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATa                                                                                                                      >  2:2901089/1‑150 (MQ=255)
                                   tattatTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACtg                                                                                                                   >  2:1750918/1‑150 (MQ=255)
                                          ttttGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCtttt                                                                                                            <  2:4107063/150‑1 (MQ=255)
                                           tttGGAGTTTTGATGCGATGCTATAAATTGAATAATTAAAACATTGTTGCATATTCAAAGGATAATTAACTGGTTTGTGTTTGTGATATTTATTATCAGTATGCTTAAAAATCAAAATAAAATTAAACATGTTTAAATACTGTACTTTTa                                                                                                           >  2:3134653/1‑150 (MQ=255)
                                           tttGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTa                                                                                                           >  2:2819765/1‑150 (MQ=255)
                                             tGGAGTTTGGTTGCGCTGATATAAATTGAATAATTAAATCTTTGTTGCATAGTCATAGGATATTTATCTGGTTTGTGTTTGTGATTTTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCTACATGTTGAAATACTGTGCTTTTATg                                                                                                         <  1:2960125/150‑1 (MQ=255)
                                              ggAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGa                                                                                                        >  2:2278662/1‑150 (MQ=255)
                                                aGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAAt                                                                                                      >  1:3378313/1‑150 (MQ=255)
                                                     ggTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATTCTACAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGAt                                                                                                 >  1:2995645/1‑150 (MQ=255)
                                                        tGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCg                                                                                              >  1:1436701/1‑150 (MQ=255)
                                                         gcgcTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCgt                                                                                             <  2:77006/150‑1 (MQ=255)
                                                           gctgctATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCgtgt                                                                                           >  1:4630458/1‑150 (MQ=255)
                                                             tgctATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGttt                                                                                         >  2:3199886/1‑150 (MQ=255)
                                                              gctATACATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGtttt                                                                                        <  2:3418416/150‑1 (MQ=255)
                                                                tataAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTtc                                                                                      <  1:530568/150‑1 (MQ=255)
                                                                  taAATTGAATATTTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTtctc                                                                                    >  2:3520769/1‑150 (MQ=255)
                                                                  taAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTtctc                                                                                    >  1:3867649/1‑150 (MQ=255)
                                                                   aaaTTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTcc                                                                                   <  2:1410114/150‑1 (MQ=255)
                                                                    aaTTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCa                                                                                  >  1:976411/1‑150 (MQ=255)
                                                                         aataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGACTTTGATGCGTGTTTTTCTCCATAAAt                                                                             >  2:3532826/1‑150 (MQ=255)
                                                                          ataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAGTTTGATGCGTGTTTTTCTCCATAAAtt                                                                            >  2:2171863/1‑150 (MQ=255)
                                                                          ataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAGTTTGATGCGTGTTTTTCTCCATAAAtt                                                                            >  2:3213716/1‑150 (MQ=255)
                                                                          ataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAAtt                                                                            >  1:3839379/1‑150 (MQ=255)
                                                                          ataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAAtt                                                                            <  2:1576142/150‑1 (MQ=255)
                                                                            aatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATtat                                                                          >  2:3860478/1‑150 (MQ=255)
                                                                            aatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATtat                                                                          >  1:3174072/1‑150 (MQ=255)
                                                                              ttAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATtatat                                                                        >  1:2881635/1‑150 (MQ=255)
                                                                               tAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATtatata                                                                       >  1:4573957/1‑150 (MQ=255)
                                                                               tAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATtatata                                                                       >  1:2328625/1‑150 (MQ=255)
                                                                                aaaaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTatatat                                                                      >  2:4119489/1‑150 (MQ=255)
                                                                                  aaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGt                                                                    >  2:3394044/1‑150 (MQ=255)
                                                                                  aaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGt                                                                    >  2:3502147/1‑150 (MQ=255)
                                                                                  aaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGt                                                                    >  2:1545810/1‑150 (MQ=255)
                                                                                  aaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGt                                                                    >  2:1545832/1‑150 (MQ=255)
                                                                                  aaCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGt                                                                    >  1:1905047/1‑150 (MQ=255)
                                                                                   aCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTTTTTGTGATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGTTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTc                                                                   <  2:3364226/150‑1 (MQ=255)
                                                                                   aCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTc                                                                   <  1:1024939/150‑1 (MQ=255)
                                                                                     tttGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCca                                                                 <  2:1054468/150‑1 (MQ=255)
                                                                                       tgttgCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCcaca                                                               >  1:1251289/1‑150 (MQ=255)
                                                                                         ttgCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACAtt                                                             <  1:1055474/150‑1 (MQ=255)
                                                                                          tgCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTc                                                            >  2:3258699/1‑150 (MQ=255)
                                                                                           gCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCg                                                           <  1:1597504/150‑1 (MQ=255)
                                                                                           gCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCg                                                           >  2:4405022/1‑150 (MQ=255)
                                                                                           gCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCg                                                           >  2:2966595/1‑150 (MQ=255)
                                                                                              tatTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGAc                                                        <  1:3705123/150‑1 (MQ=255)
                                                                                              tatTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGAc                                                        <  1:3705096/150‑1 (MQ=255)
                                                                                              tatTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGAc                                                        >  1:1457265/1‑150 (MQ=255)
                                                                                               atTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACt                                                       >  1:1719180/1‑150 (MQ=255)
                                                                                                   aTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAgg                                                   >  1:869396/1‑150 (MQ=255)
                                                                                                   aTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAgg                                                   >  1:1918596/1‑150 (MQ=255)
                                                                                                   aTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAgg                                                   >  1:2917769/1‑150 (MQ=255)
                                                                                                    tAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACGTAggg                                                  >  2:100459/1‑150 (MQ=255)
                                                                                                      ggATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGa                                                >  2:4240224/1‑150 (MQ=255)
                                                                                                         tatTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAg                                             >  1:3106543/1‑150 (MQ=255)
                                                                                                         tatTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAg                                             >  2:730960/1‑150 (MQ=255)
                                                                                                              aTCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGaataa                                        >  2:1285166/1‑150 (MQ=255)
                                                                                                                cTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTTAAATACTGAGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAAtt                                      >  2:4305123/1‑150 (MQ=255)
                                                                                                                  ggTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGa                                    >  1:2587099/1‑150 (MQ=255)
                                                                                                                      tgtgtttgtgATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCa                                <  1:3216721/150‑1 (MQ=255)
                                                                                                                         ttttgtgATATTTATTATGCGTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTc                             <  1:4342666/149‑1 (MQ=255)
                                                                                                                         gtttttgATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTc                             <  1:4567431/150‑1 (MQ=255)
                                                                                                                             gtgATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCt                         <  2:1826162/150‑1 (MQ=255)
                                                                                                                                  atttattATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAAc                    <  1:4363373/150‑1 (MQ=255)
                                                                                                                                       ttatGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACgcagc               <  1:3102698/150‑1 (MQ=255)
                                                                                                                                            cGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAAt          >  1:3672562/1‑150 (MQ=255)
                                                                                                                                             gTATGCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATc         <  1:3392540/150‑1 (MQ=255)
                                                                                                                                             gTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATc         <  2:1070478/150‑1 (MQ=255)
                                                                                                                                             gTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCAGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATc         <  2:1070576/150‑1 (MQ=255)
                                                                                                                                               aTGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGt       <  2:2459360/150‑1 (MQ=255)
                                                                                                                                               aTGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGt       <  2:2042173/150‑1 (MQ=255)
                                                                                                                                                tGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGtt      >  1:2416752/1‑150 (MQ=255)
                                                                                                                                                 gCTTTAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTa     <  2:2118775/150‑1 (MQ=255)
                                                                                                                                                   ttCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAgc   <  1:1815642/150‑1 (MQ=255)
                                                                                                                                                    tCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAgcg  <  2:2257872/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCG  >  CP009273/385336‑385633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A