Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,815 G→T 45.9% pseudogene (109/1458 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,8150GT45.9% 115.2 / 287.9 207pseudogene (109/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base G (59/53);  new base T (44/51);  total (103/104)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 207 total aligned reads displayed.

TGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG  >  CP009273/385667‑385960
                                                                                                                                                    |                                                                                                                                                 
tGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGa                                                                                                                                                  >  2:3708754/1‑150 (MQ=255)
tGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGa                                                                                                                                                  >  2:290905/1‑150 (MQ=255)
   tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATaa                                                                                                                                               <  1:1926338/150‑1 (MQ=255)
   tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATaa                                                                                                                                               <  1:1135983/150‑1 (MQ=255)
    ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATCAt                                                                                                                                              >  1:132883/1‑150 (MQ=255)
    ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAAt                                                                                                                                              >  1:2306086/1‑150 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                          >  2:1105615/1‑150 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCg                                                                                                                                          >  2:1900620/1‑150 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCg                                                                                                                                          <  1:303444/150‑1 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCg                                                                                                                                          <  1:599949/150‑1 (MQ=255)
         aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGt                                                                                                                                         >  2:4213915/1‑150 (MQ=255)
         aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGt                                                                                                                                         >  2:2025761/1‑150 (MQ=255)
           cTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTa                                                                                                                                       >  1:4099054/1‑150 (MQ=255)
           cTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTa                                                                                                                                       >  2:1564103/1‑150 (MQ=255)
             tattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACa                                                                                                                                     <  1:2560796/150‑1 (MQ=255)
               ttaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGc                                                                                                                                   >  2:2587599/1‑150 (MQ=255)
               ttaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGc                                                                                                                                   >  2:2099566/1‑150 (MQ=255)
                taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCa                                                                                                                                  >  1:2946808/1‑150 (MQ=255)
                  cGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACt                                                                                                                                >  1:1368915/1‑150 (MQ=255)
                       cTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAtt                                                                                                                           >  1:3947531/1‑150 (MQ=255)
                       cTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGAtt                                                                                                                           >  1:3319258/1‑150 (MQ=255)
                       cTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGAtt                                                                                                                           <  2:3500038/150‑1 (MQ=255)
                       cTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGAtt                                                                                                                           <  2:1826716/150‑1 (MQ=255)
                        tGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTg                                                                                                                          <  1:2361366/150‑1 (MQ=255)
                         gaggagGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTgg                                                                                                                         <  1:3752214/150‑1 (MQ=255)
                             aggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATc                                                                                                                     <  1:1042647/150‑1 (MQ=255)
                              gggATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCa                                                                                                                    >  2:4296119/1‑150 (MQ=255)
                                gATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCata                                                                                                                  <  2:2749233/150‑1 (MQ=255)
                                  tGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATg                                                                                                                >  1:3817069/1‑150 (MQ=255)
                                    aCAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGa                                                                                                              >  1:1787281/1‑150 (MQ=255)
                                      aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                            >  2:829108/1‑150 (MQ=255)
                                      aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                            >  2:1290269/1‑150 (MQ=255)
                                            aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcag                                                                                                      >  1:1478978/1‑150 (MQ=255)
                                                cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAc                                                                                                  >  1:47635/1‑150 (MQ=255)
                                                  ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                                <  2:1107110/150‑1 (MQ=255)
                                                   gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                               >  2:888908/1‑150 (MQ=255)
                                                    aaaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATCTCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTa                                                                                              >  2:4158623/1‑150 (MQ=255)
                                                    aaaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGCTCATATGGATAAGGCAGGCACTGTa                                                                                              >  2:586045/1‑150 (MQ=255)
                                                     aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAg                                                                                             >  1:2519336/1‑150 (MQ=255)
                                                             cTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAc                                                                                     >  2:3780027/1‑150 (MQ=255)
                                                               tGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTa                                                                                   <  2:3237381/150‑1 (MQ=255)
                                                                 tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                 >  1:4148591/1‑150 (MQ=255)
                                                                 tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                 <  1:874637/150‑1 (MQ=255)
                                                                  agtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGt                                                                                <  2:3569635/150‑1 (MQ=255)
                                                                   gtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTa                                                                               >  2:2833057/1‑150 (MQ=255)
                                                                    taTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                              >  1:2917194/1‑150 (MQ=255)
                                                                      tCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                            <  2:3553184/150‑1 (MQ=255)
                                                                       cacaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                           >  2:858636/1‑150 (MQ=255)
                                                                       cacaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                           <  2:3299443/150‑1 (MQ=255)
                                                                        acaATTATCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTTATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACta                                                                          <  2:2829772/150‑1 (MQ=255)
                                                                        acaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACta                                                                          <  1:2116013/150‑1 (MQ=255)
                                                                             tAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTg                                                                     <  2:2629887/150‑1 (MQ=255)
                                                                                cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGAACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                  <  1:2414668/150‑1 (MQ=255)
                                                                                 aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                 >  2:3681784/1‑150 (MQ=255)
                                                                                  ttGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtttt                                                                <  1:993465/150‑1 (MQ=255)
                                                                                     gCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTc                                                             <  1:2701357/150‑1 (MQ=255)
                                                                                      cTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCt                                                            <  2:1918042/150‑1 (MQ=255)
                                                                                        tGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTAt                                                          <  1:3560737/150‑1 (MQ=255)
                                                                                           aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                       <  1:3369667/150‑1 (MQ=255)
                                                                                           aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                       <  1:3519904/150‑1 (MQ=255)
                                                                                              ctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaac                                                    <  1:1057177/150‑1 (MQ=255)
                                                                                               tGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaaca                                                   >  2:1950154/1‑150 (MQ=255)
                                                                                                ggCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCCGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaa                                                  >  2:471529/1‑150 (MQ=255)
                                                                                                    aTCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAcc                                              >  1:2181514/1‑150 (MQ=255)
                                                                                                    aTCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATACGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAcc                                              >  2:3165777/1‑150 (MQ=255)
                                                                                                      cACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCaa                                            >  1:3994211/1‑150 (MQ=255)
                                                                                                        ccTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATg                                          >  2:960535/1‑150 (MQ=255)
                                                                                                         gccccatgCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                         <  1:592110/143‑1 (MQ=255)
                                                                                                         ctctCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                         <  2:1368915/150‑1 (MQ=255)
                                                                                                         ctctCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                         >  1:2456675/1‑150 (MQ=255)
                                                                                                          tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                        <  1:697629/150‑1 (MQ=255)
                                                                                                          tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                        <  1:1766423/150‑1 (MQ=255)
                                                                                                          tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                        <  1:2062296/150‑1 (MQ=255)
                                                                                                          tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                        <  2:4037553/150‑1 (MQ=255)
                                                                                                           ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTg                                       >  1:2584711/1‑150 (MQ=255)
                                                                                                            tcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGa                                      <  2:239178/150‑1 (MQ=255)
                                                                                                             cAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAt                                     >  2:2304877/1‑150 (MQ=255)
                                                                                                             cAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAt                                     <  1:856866/150‑1 (MQ=255)
                                                                                                              aGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAtt                                    <  2:4116519/150‑1 (MQ=255)
                                                                                                                 cTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCg                                 <  2:2946808/150‑1 (MQ=255)
                                                                                                                 cTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCg                                 >  1:4243436/1‑150 (MQ=255)
                                                                                                                  tAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGa                                >  1:1712888/1‑150 (MQ=255)
                                                                                                                     tGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                             <  1:3107264/150‑1 (MQ=255)
                                                                                                                      gcagcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAg                            <  1:1773571/150‑1 (MQ=255)
                                                                                                                        agcaAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                          >  2:3000175/1‑150 (MQ=255)
                                                                                                                           aaaCTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCtt                       <  1:907089/150‑1 (MQ=255)
                                                                                                                            aaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGTTGATTTCGATCAGTCCttt                      <  2:3109449/150‑1 (MQ=255)
                                                                                                                             aCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTa                     >  2:4063508/1‑150 (MQ=255)
                                                                                                                               tATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAAc                   >  2:1633606/1‑150 (MQ=255)
                                                                                                                               tATGATACCTGGACTTATATCGATAAAACCGTTACAGAACTTGAAAGCGATCATATGTATAAGGCATGCACTGTATATGGAAACTATTTAAACTATAGTGGTTTTGTCTATTACAACAAAAACAATGGTGATTTCTATCAGTCATTTAAc                   >  1:2740644/1‑150 (MQ=255)
                                                                                                                                aTGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                  <  2:2366425/150‑1 (MQ=255)
                                                                                                                                aTGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                  >  2:3965553/1‑150 (MQ=255)
                                                                                                                                  gATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGc                <  2:1228026/150‑1 (MQ=255)
                                                                                                                                   aTACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg               <  1:3648781/150‑1 (MQ=255)
                                                                                                                                     aCCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAt             >  1:1790385/1‑150 (MQ=255)
                                                                                                                                       cTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAc           <  2:3099941/150‑1 (MQ=255)
                                                                                                                                        tGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAcc          <  2:780648/150‑1 (MQ=255)
                                                                                                                                        tGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAcc          >  1:3069955/1‑150 (MQ=255)
                                                                                                                                           aCTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGtt       <  1:2095317/150‑1 (MQ=255)
                                                                                                                                               atatCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACg   <  2:1928213/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG  >  CP009273/385667‑385960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  CP009273/385666‑385964
                                                                                                                                                     |                                                                                                                                                     
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCT                                                                                                                                                       <  SRR19973079.819390/150‑1 (MQ=60)
   ATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATA                                                                                                                                                    >  SRR19973079.406212/1‑150 (MQ=60)
    TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAA                                                                                                                                                   <  SRR19973079.1936629/150‑1 (MQ=60)
    TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAA                                                                                                                                                   <  SRR19973079.1936487/150‑1 (MQ=60)
    TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAA                                                                                                                                                   <  SRR19973079.1142490/150‑1 (MQ=60)
     GGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATCAT                                                                                                                                                  >  SRR19973079.133958/1‑150 (MQ=60)
     GGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAAT                                                                                                                                                  >  SRR19973079.2318054/1‑150 (MQ=60)
       ATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCC                                                                                                                                                <  SRR19973079.234035/150‑1 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCG                                                                                                                                              <  SRR19973079.603441/150‑1 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCG                                                                                                                                              <  SRR19973079.2489639/150‑1 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCG                                                                                                                                              <  SRR19973079.304876/150‑1 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCG                                                                                                                                              <  SRR19973079.305231/150‑1 (MQ=60)
           ACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACGTATATCTATCATCCCGTT                                                                                                                                            >  SRR19973079.3574875/1‑150 (MQ=60)
            CTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTA                                                                                                                                           >  SRR19973079.4119833/1‑150 (MQ=60)
              TATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACA                                                                                                                                         <  SRR19973079.2573816/150‑1 (MQ=60)
              TATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACA                                                                                                                                         <  SRR19973079.389196/150‑1 (MQ=60)
                 TACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCA                                                                                                                                      >  SRR19973079.2961920/1‑150 (MQ=60)
                 TACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCA                                                                                                                                      >  SRR19973079.1364183/1‑150 (MQ=60)
                   CGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACT                                                                                                                                    >  SRR19973079.1376511/1‑150 (MQ=60)
                        CTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATT                                                                                                                               >  SRR19973079.3967440/1‑150 (MQ=60)
                        CTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATT                                                                                                                               >  SRR19973079.3336224/1‑150 (MQ=60)
                        CTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATT                                                                                                                               >  SRR19973079.3336244/1‑150 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973079.2373615/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973079.1786545/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973079.3458359/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973079.3984660/150‑1 (MQ=60)
                          GAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGG                                                                                                                             <  SRR19973079.3771434/150‑1 (MQ=60)
                              AGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATC                                                                                                                         <  SRR19973079.1048481/150‑1 (MQ=60)
                              AGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATC                                                                                                                         <  SRR19973079.1048413/150‑1 (MQ=60)
                                   TGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATG                                                                                                                    >  SRR19973079.3836603/1‑150 (MQ=60)
                                     ACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACgtgttggggatcagaggga                                                                                                                  >  SRR19973079.3297827/1‑131 (MQ=60)
                                     ACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGA                                                                                                                  >  SRR19973079.1796621/1‑150 (MQ=60)
                                             ACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAG                                                                                                          >  SRR19973079.3711077/1‑150 (MQ=60)
                                             ACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAG                                                                                                          >  SRR19973079.1487062/1‑150 (MQ=60)
                                             ACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAG                                                                                                          >  SRR19973079.1486937/1‑150 (MQ=60)
                                             ACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAG                                                                                                          <  SRR19973079.2185470/150‑1 (MQ=60)
                                              CTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATACTCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGG                                                                                                         <  SRR19973079.1522523/150‑1 (MQ=60)
                                                CCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCA                                                                                                       >  SRR19973079.310482/1‑150 (MQ=60)
                                                 CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                      >  SRR19973079.48123/1‑150 (MQ=60)
                                                  TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACT                                                                                                     <  SRR19973079.688858/150‑1 (MQ=60)
                                                    GAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGT                                                                                                   <  SRR19973079.2745812/150‑1 (MQ=60)
                                                      AAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAG                                                                                                 >  SRR19973079.2532243/1‑150 (MQ=60)
                                                      AAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAG                                                                                                 >  SRR19973079.2532074/1‑150 (MQ=60)
                                                         AGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATG                                                                                              <  SRR19973079.426738/150‑1 (MQ=60)
                                                                  TAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATG                                                                                     >  SRR19973079.4169647/1‑150 (MQ=60)
                                                                  TAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATG                                                                                     <  SRR19973079.879704/150‑1 (MQ=60)
                                                                   AGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGT                                                                                    >  SRR19973079.972255/1‑150 (MQ=60)
                                                                    GTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTA                                                                                   >  SRR19973079.749292/1‑150 (MQ=60)
                                                                     TATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAA                                                                                  >  SRR19973079.2932013/1‑150 (MQ=60)
                                                                     TATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAA                                                                                  <  SRR19973079.2312673/150‑1 (MQ=60)
                                                                        CACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACT                                                                               <  SRR19973079.3777963/150‑1 (MQ=60)
                                                                         ACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTA                                                                              <  SRR19973079.2126860/150‑1 (MQ=60)
                                                                         ACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTA                                                                              <  SRR19973079.2126974/150‑1 (MQ=60)
                                                                            ATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAG                                                                           >  SRR19973079.2448194/1‑150 (MQ=60)
                                                                            ATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAG                                                                           >  SRR19973079.2645382/1‑150 (MQ=60)
                                                                             TTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGT                                                                          <  SRR19973079.1986661/150‑1 (MQ=60)
                                                                                 CATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGAACTGTAGATGGCAACTATGTAAACTATAGTGGTT                                                                      <  SRR19973079.2427116/150‑1 (MQ=60)
                                                                                   TTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTT                                                                    <  SRR19973079.999080/150‑1 (MQ=60)
                                                                                      GCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTC                                                                 <  SRR19973079.2715346/150‑1 (MQ=60)
                                                                                         TGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTAT                                                              <  SRR19973079.3578932/150‑1 (MQ=60)
                                                                                          GCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATT                                                             <  SRR19973079.173638/150‑1 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           <  SRR19973079.3387020/150‑1 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           <  SRR19973079.3537983/150‑1 (MQ=60)
                                                                                               CTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAAC                                                        <  SRR19973079.1062972/150‑1 (MQ=60)
                                                                                                     ATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACC                                                  >  SRR19973079.2192680/1‑150 (MQ=60)
                                                                                                       CACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAA                                                >  SRR19973079.4014250/1‑150 (MQ=60)
                                                                                                          gccccatGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGG                                             <  SRR19973079.595661/143‑1 (MQ=60)
                                                                                                          gccccatGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGG                                             <  SRR19973079.595573/143‑1 (MQ=60)
                                                                                                          CTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGG                                             >  SRR19973079.2469268/1‑150 (MQ=60)
                                                                                                           TCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                            <  SRR19973079.701413/150‑1 (MQ=60)
                                                                                                           TCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                            <  SRR19973079.1775713/150‑1 (MQ=60)
                                                                                                           TCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                            <  SRR19973079.2073099/150‑1 (MQ=60)
                                                                                                           TCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                            <  SRR19973079.2382962/150‑1 (MQ=60)
                                                                                                           TCTCAGGCTAATGCAGCAAACTATGAGACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                            <  SRR19973079.2073113/150‑1 (MQ=60)
                                                                                                            CTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTG                                           >  SRR19973079.2596179/1‑150 (MQ=60)
                                                                                                            CTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTG                                           >  SRR19973079.2597953/1‑150 (MQ=60)
                                                                                                              CAGGCTAATGGAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAT                                         <  SRR19973079.863591/150‑1 (MQ=60)
                                                                                                              CAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAT                                         <  SRR19973079.861815/150‑1 (MQ=60)
                                                                                                               AGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATT                                        >  SRR19973079.483098/1‑150 (MQ=60)
                                                                                                                  CTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCG                                     >  SRR19973079.4264907/1‑150 (MQ=60)
                                                                                                                   TAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGA                                    >  SRR19973079.1722037/1‑150 (MQ=60)
                                                                                                                      TGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCA                                 <  SRR19973079.3123299/150‑1 (MQ=60)
                                                                                                                       GCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAG                                <  SRR19973079.1782874/150‑1 (MQ=60)
                                                                                                                       GCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAG                                <  SRR19973079.1782766/150‑1 (MQ=60)
                                                                                                                            AAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTT                           <  SRR19973079.3108128/150‑1 (MQ=60)
                                                                                                                            AAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTT                           <  SRR19973079.912335/150‑1 (MQ=60)
                                                                                                                                TATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAAC                       >  SRR19973079.572442/1‑150 (MQ=60)
                                                                                                                                TATGATACCTGGACTTATATCGATAAAACCGTTACAGAACTTGAAAGCGATCATATGTATAAGGCATGCACTGTATATGGAAACTATTTAAACTATAGTGGTTTTGTCTATTACAACAAAAACAATGGTGATTTCTATCAGTCATTTAAC                       >  SRR19973079.2754792/1‑150 (MQ=60)
                                                                                                                                    ATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCG                   <  SRR19973079.3667322/150‑1 (MQ=60)
                                                                                                                                      ACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAT                 >  SRR19973079.1799583/1‑150 (MQ=60)
                                                                                                                                      ACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAT                 >  SRR19973079.1799730/1‑150 (MQ=60)
                                                                                                                                         TGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACC              >  SRR19973079.1210014/1‑150 (MQ=60)
                                                                                                                                         TGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACC              >  SRR19973079.3085848/1‑150 (MQ=60)
                                                                                                                                         TGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACC              >  SRR19973079.893028/1‑150 (MQ=60)
                                                                                                                                           GACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGT            >  SRR19973079.2187555/1‑150 (MQ=60)
                                                                                                                                            ACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTT           <  SRR19973079.2106220/150‑1 (MQ=60)
                                                                                                                                            ACTTATATCTATAATCCCGTTACAGAAATTTATTGGGATCATTTGGATAAGGCAGGCACTGTATTTGGAAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCATTGGTGATTTCGATAAGTCCTTTAACGGCGATACCGTT           <  SRR19973079.4151362/150‑1 (MQ=60)
                                                                                                                                            ACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTT           >  SRR19973079.2840393/1‑150 (MQ=60)
                                                                                                                                              TTATATCTATAATCCCGTTACAGCACTTTATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATTGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAA         <  SRR19973079.2937931/150‑1 (MQ=60)
                                                                                                                                              TTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAA         <  SRR19973079.3059981/150‑1 (MQ=60)
                                                                                                                                               TATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC        >  SRR19973079.4283042/1‑150 (MQ=60)
                                                                                                                                               TATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC        >  SRR19973079.107926/1‑150 (MQ=60)
                                                                                                                                               TATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC        <  SRR19973079.3593027/150‑1 (MQ=60)
                                                                                                                                                    CTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCAC   <  SRR19973079.498431/150‑1 (MQ=60)
                                                                                                                                                    CTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCAC   <  SRR19973079.3878768/150‑1 (MQ=60)
                                                                                                                                                     TATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  SRR19973079.833461/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  CP009273/385666‑385964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑