Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,815 G→T 100% pseudogene (109/1458 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,8150GT98.1% 662.5 / 1.5 207pseudogene (109/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (110/92);  total (114/93)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

GATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC  >  CP009273/385668‑385958
                                                                                                                                                   |                                                                                                                                               
 aTGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCtata                                                                                                                                              >  2:1281116/1‑150 (MQ=255)
   ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAAt                                                                                                                                            >  2:1352860/1‑150 (MQ=255)
   ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAAt                                                                                                                                            >  2:1804601/1‑150 (MQ=255)
    gATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATc                                                                                                                                           >  2:2680525/1‑150 (MQ=255)
     aTCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATcc                                                                                                                                          <  2:1761354/150‑1 (MQ=255)
      tCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                         <  1:2789570/150‑1 (MQ=255)
      tCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                         >  1:4240887/1‑150 (MQ=255)
      tCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                         >  1:4241120/1‑150 (MQ=255)
      tCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                         <  1:3796882/150‑1 (MQ=255)
       cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                        >  1:1030329/1‑150 (MQ=255)
       cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                        <  2:1329470/150‑1 (MQ=255)
        aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGt                                                                                                                                       >  2:1425656/1‑150 (MQ=255)
        aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTGGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGt                                                                                                                                       >  2:3096708/1‑150 (MQ=255)
         aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGtt                                                                                                                                      >  2:2001301/1‑150 (MQ=255)
         aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGtt                                                                                                                                      <  2:2225818/150‑1 (MQ=255)
          cTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTa                                                                                                                                     <  1:682801/150‑1 (MQ=255)
           ttattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTAc                                                                                                                                    <  2:4459158/150‑1 (MQ=255)
              ttaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGc                                                                                                                                 <  2:1414004/150‑1 (MQ=255)
              ttaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGc                                                                                                                                 >  2:4836921/1‑150 (MQ=255)
               taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCa                                                                                                                                >  1:3505803/1‑150 (MQ=255)
               taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCa                                                                                                                                >  1:3442860/1‑150 (MQ=255)
               taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGCCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCa                                                                                                                                >  2:1488777/1‑150 (MQ=255)
                   tCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTg                                                                                                                            <  1:3942055/150‑1 (MQ=255)
                    cccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGa                                                                                                                           <  1:3255648/150‑1 (MQ=255)
                     ccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAt                                                                                                                          <  2:122949/150‑1 (MQ=255)
                       tGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTg                                                                                                                        <  1:3807378/150‑1 (MQ=255)
                       tGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTg                                                                                                                        >  2:538042/1‑150 (MQ=255)
                        gaggagGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTgg                                                                                                                       <  1:2234177/150‑1 (MQ=255)
                        gaggagGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTgg                                                                                                                       >  2:3607432/1‑150 (MQ=255)
                        gaggagGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTgg                                                                                                                       >  2:2858230/1‑150 (MQ=255)
                          ggaggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGa                                                                                                                     >  2:3684532/1‑150 (MQ=255)
                           gaggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGAt                                                                                                                    <  2:2127494/150‑1 (MQ=255)
                            aggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATc                                                                                                                   <  2:3956322/150‑1 (MQ=255)
                            aggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCAATAATCCCGTTACAGCACTTGATTGGGATc                                                                                                                   >  1:4726524/1‑150 (MQ=255)
                              ggATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCat                                                                                                                 <  1:4652875/150‑1 (MQ=255)
                                 tGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATg                                                                                                              >  2:2197718/1‑150 (MQ=255)
                                 tGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATg                                                                                                              <  1:1096159/150‑1 (MQ=255)
                                    cAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGAt                                                                                                           >  1:4332935/1‑150 (MQ=255)
                                     aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                          >  1:4574065/1‑150 (MQ=255)
                                           aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcag                                                                                                    <  2:454268/150‑1 (MQ=255)
                                            cTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAgggcagg                                                                                                   <  1:3611676/150‑1 (MQ=255)
                                            cTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcagg                                                                                                   >  1:153155/1‑150 (MQ=255)
                                            cTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcagg                                                                                                   >  1:1421912/1‑150 (MQ=255)
                                              ccTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAggcaggca                                                                                                 >  2:3510875/1‑150 (MQ=255)
                                               cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAc                                                                                                <  1:2416132/150‑1 (MQ=255)
                                                tGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATCCCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACt                                                                                               >  1:1992791/1‑150 (MQ=255)
                                                tGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACt                                                                                               >  2:4070286/1‑150 (MQ=255)
                                                 ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                              <  1:2733975/150‑1 (MQ=255)
                                                 ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                              >  1:4469650/1‑150 (MQ=255)
                                                  gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                             <  1:63122/150‑1 (MQ=255)
                                                  gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                             <  1:63224/150‑1 (MQ=255)
                                                    aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAg                                                                                           <  2:2613821/150‑1 (MQ=255)
                                                      aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                         >  1:2075281/1‑150 (MQ=255)
                                                      aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                         >  1:3995951/1‑150 (MQ=255)
                                                          aaCTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCa                                                                                     >  2:2677237/1‑150 (MQ=255)
                                                           aCTTTTAGTATCACTATTAGCATTGGCTTGCTCTCTTGCTATCTCCTCTCAGGCTAATGCAGCAAACTATGATACCTTGACTTATATCTATAATCCCGTTACAGAACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCaa                                                                                    <  1:505967/150‑1 (MQ=255)
                                                           aCTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCaa                                                                                    <  1:2130816/150‑1 (MQ=255)
                                                                tagtaTCACAATTAGCATTGGCTTGCAGTCTGGCTATCACCTCTCAGGATATTGCATCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGTGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                               <  1:478575/150‑1 (MQ=255)
                                                                 agtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGt                                                                              >  2:4043099/1‑150 (MQ=255)
                                                                  gtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTa                                                                             >  2:1018141/1‑150 (MQ=255)
                                                                     tCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                          <  1:1080784/150‑1 (MQ=255)
                                                                      cacaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                         <  2:361983/150‑1 (MQ=255)
                                                                      cacaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                         <  1:2974786/150‑1 (MQ=255)
                                                                             aGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTgg                                                                  <  2:1899233/150‑1 (MQ=255)
                                                                               cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                >  2:205790/1‑150 (MQ=255)
                                                                                aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                               >  2:85639/1‑150 (MQ=255)
                                                                                  tGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTg                                                             <  1:4096371/150‑1 (MQ=255)
                                                                                  tGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTg                                                             >  2:2494187/1‑150 (MQ=255)
                                                                                   ggCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGt                                                            >  2:4120076/1‑150 (MQ=255)
                                                                                     cTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCt                                                          >  2:2454688/1‑150 (MQ=255)
                                                                                      ttGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTa                                                         >  2:4301191/1‑150 (MQ=255)
                                                                                          aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                     >  1:3323925/1‑150 (MQ=255)
                                                                                          aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                     >  1:841588/1‑150 (MQ=255)
                                                                                          aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                     >  1:1819027/1‑150 (MQ=255)
                                                                                             ctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaac                                                  >  2:3382918/1‑150 (MQ=255)
                                                                                                gCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaac                                               <  1:4959139/150‑1 (MQ=255)
                                                                                                 cTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaaca                                              >  1:1449492/1‑150 (MQ=255)
                                                                                                  tATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAacac                                             >  1:1684492/1‑150 (MQ=255)
                                                                                                  tATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAacac                                             <  2:29271/150‑1 (MQ=255)
                                                                                                  tATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAacac                                             >  1:4804766/1‑150 (MQ=255)
                                                                                                         tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                      <  2:1143709/150‑1 (MQ=255)
                                                                                                               gCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTc                                <  1:4327749/150‑1 (MQ=255)
                                                                                                                  aaTGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAGGGCAACTATGTTAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGAt                             <  2:5043058/150‑1 (MQ=255)
                                                                                                                   aTGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATc                            >  2:1742676/1‑150 (MQ=255)
                                                                                                                   aTGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATc                            >  1:1595536/1‑150 (MQ=255)
                                                                                                                    tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                           <  2:961593/150‑1 (MQ=255)
                                                                                                                      cagcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGt                         >  1:3835128/1‑150 (MQ=255)
                                                                                                                       agcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                        >  2:5102980/1‑150 (MQ=255)
                                                                                                                           aaCTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCttt                    >  1:3808940/1‑150 (MQ=255)
                                                                                                                             cTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTaa                  <  1:2257790/150‑1 (MQ=255)
                                                                                                                               aTGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGTCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                <  1:595282/150‑1 (MQ=255)
                                                                                                                               aTGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                <  1:1281116/150‑1 (MQ=255)
                                                                                                                                tGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACgg               >  2:4272289/1‑150 (MQ=255)
                                                                                                                                tGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACgg               <  2:4753988/150‑1 (MQ=255)
                                                                                                                                    aCCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAt           <  2:459427/150‑1 (MQ=255)
                                                                                                                                     ccTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATa          >  1:2921869/1‑150 (MQ=255)
                                                                                                                                       tGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAcc        >  2:2788938/1‑150 (MQ=255)
                                                                                                                                        ggACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCg       <  2:1366314/150‑1 (MQ=255)
                                                                                                                                            ttATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa   <  2:4726524/150‑1 (MQ=255)
                                                                                                                                            ttATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa   <  2:1316914/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC  >  CP009273/385668‑385958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  CP009273/385666‑385964
                                                                                                                                                     |                                                                                                                                                     
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCT                                                                                                                                                       <  SRR19973063.4620109/150‑1 (MQ=60)
  GATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTAT                                                                                                                                                     >  SRR19973063.2171200/1‑150 (MQ=60)
      GATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATC                                                                                                                                                 >  SRR19973063.4707356/1‑150 (MQ=60)
      GATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATC                                                                                                                                                 <  SRR19973063.1177890/150‑1 (MQ=60)
      GATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATC                                                                                                                                                 <  SRR19973063.1442115/150‑1 (MQ=60)
        TCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCC                                                                                                                                               >  SRR19973063.4254407/1‑150 (MQ=60)
        TCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCC                                                                                                                                               >  SRR19973063.4254590/1‑150 (MQ=60)
        TCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCC                                                                                                                                               <  SRR19973063.2798549/150‑1 (MQ=60)
        TCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCC                                                                                                                                               >  SRR19973063.4254640/1‑150 (MQ=60)
        TCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCC                                                                                                                                               <  SRR19973063.3808983/150‑1 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCG                                                                                                                                              >  SRR19973063.1033610/1‑150 (MQ=60)
         CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCG                                                                                                                                              >  SRR19973063.1033439/1‑150 (MQ=60)
            CTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTA                                                                                                                                           <  SRR19973063.684802/150‑1 (MQ=60)
            CTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTA                                                                                                                                           <  SRR19973063.2755657/150‑1 (MQ=60)
                 TACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCA                                                                                                                                      >  SRR19973063.3517015/1‑150 (MQ=60)
                 TACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCA                                                                                                                                      >  SRR19973063.3453808/1‑150 (MQ=60)
                   CGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACT                                                                                                                                    <  SRR19973063.4277623/150‑1 (MQ=60)
                     TCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTG                                                                                                                                  <  SRR19973063.3954659/150‑1 (MQ=60)
                      CCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGA                                                                                                                                 <  SRR19973063.3266093/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973063.3819458/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              <  SRR19973063.3819501/150‑1 (MQ=60)
                         TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                              >  SRR19973063.4769142/1‑150 (MQ=60)
                          GAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGG                                                                                                                             <  SRR19973063.2241278/150‑1 (MQ=60)
                          GAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGG                                                                                                                             <  SRR19973063.2241263/150‑1 (MQ=60)
                              AGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCAATAATCCCGTTACAGCACTTGATAGGGATC                                                                                                                         >  SRR19973063.4741590/1‑150 (MQ=60)
                                GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCAT                                                                                                                       <  SRR19973063.4667731/150‑1 (MQ=60)
                                GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCAT                                                                                                                       >  SRR19973063.4212311/1‑150 (MQ=60)
                                   TGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATG                                                                                                                    <  SRR19973063.1099495/150‑1 (MQ=60)
                                    GACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGG                                                                                                                   >  SRR19973063.2082985/1‑150 (MQ=60)
                                    GACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGG                                                                                                                   >  SRR19973063.662242/1‑150 (MQ=60)
                                      CAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGAT                                                                                                                 >  SRR19973063.4346740/1‑150 (MQ=60)
                                      CAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGAT                                                                                                                 >  SRR19973063.4346732/1‑150 (MQ=60)
                                       AAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATA                                                                                                                >  SRR19973063.4588696/1‑150 (MQ=60)
                                              CTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAGGGCAGG                                                                                                         <  SRR19973063.1964140/150‑1 (MQ=60)
                                              CTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAGGGCAGG                                                                                                         <  SRR19973063.3623272/150‑1 (MQ=60)
                                              CTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGG                                                                                                         >  SRR19973063.1426237/1‑150 (MQ=60)
                                              CTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGG                                                                                                         >  SRR19973063.153623/1‑150 (MQ=60)
                                                CCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCA                                                                                                       >  SRR19973063.1320843/1‑150 (MQ=60)
                                                CCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCA                                                                                                       >  SRR19973063.480333/1‑150 (MQ=60)
                                                 CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCTTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                      <  SRR19973063.2405156/150‑1 (MQ=60)
                                                 CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                      <  SRR19973063.2423857/150‑1 (MQ=60)
                                                  TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATCCCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGTATAAGGCAGGCACT                                                                                                     >  SRR19973063.1999019/1‑150 (MQ=60)
                                                  TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACT                                                                                                     >  SRR19973063.1095811/1‑150 (MQ=60)
                                                  TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGCCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACT                                                                                                     <  SRR19973063.1493336/150‑1 (MQ=60)
                                                   GGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTG                                                                                                    >  SRR19973063.4483986/1‑150 (MQ=60)
                                                   GGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTG                                                                                                    <  SRR19973063.2742799/150‑1 (MQ=60)
                                                    GAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGT                                                                                                   <  SRR19973063.63215/150‑1 (MQ=60)
                                                    GAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGT                                                                                                   <  SRR19973063.63340/150‑1 (MQ=60)
                                                    GAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGT                                                                                                   <  SRR19973063.63376/150‑1 (MQ=60)
                                                    GAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGT                                                                                                   <  SRR19973063.63442/150‑1 (MQ=60)
                                                        AAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAT                                                                                               >  SRR19973063.4008753/1‑150 (MQ=60)
                                                        AAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAT                                                                                               >  SRR19973063.2081769/1‑150 (MQ=60)
                                                        AAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAT                                                                                               >  SRR19973063.4008766/1‑150 (MQ=60)
                                                           AAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGC                                                                                            <  SRR19973063.2801991/150‑1 (MQ=60)
                                                             ACTTTTAGTATCACTATTAGCATTGGCTTGCTCTCTTGCTATCTCCTCTCAGGCTAATGCAGCAAACTATGATACCTTGACTTATATCTATAATCCCGTTACAGAACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAA                                                                                          <  SRR19973063.507434/150‑1 (MQ=60)
                                                             ACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAA                                                                                          <  SRR19973063.2137473/150‑1 (MQ=60)
                                                             ACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAA                                                                                          <  SRR19973063.3559023/150‑1 (MQ=60)
                                                               TTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACT                                                                                        >  SRR19973063.3482973/1‑150 (MQ=60)
                                                                  TAGTATCACAATTAGCATTGGCTTGCAGTCTGGCTATCACCTCTCAGGATATTGCATCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGTGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATG                                                                                     <  SRR19973063.479933/150‑1 (MQ=60)
                                                                       TCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAC                                                                                <  SRR19973063.1084070/150‑1 (MQ=60)
                                                                        CACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACT                                                                               <  SRR19973063.2984294/150‑1 (MQ=60)
                                                                           AATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATA                                                                            >  SRR19973063.2541494/1‑150 (MQ=60)
                                                                                 CATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTT                                                                      <  SRR19973063.3823871/150‑1 (MQ=60)
                                                                                  ATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTT                                                                     <  SRR19973063.4602837/150‑1 (MQ=60)
                                                                                    TGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTG                                                                   <  SRR19973063.4108983/150‑1 (MQ=60)
                                                                                    TGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTG                                                                   <  SRR19973063.4109497/150‑1 (MQ=60)
                                                                                          GCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATT                                                             >  SRR19973063.3768711/1‑150 (MQ=60)
                                                                                          GCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATT                                                             >  SRR19973063.3819731/1‑150 (MQ=60)
                                                                                          GCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATT                                                             >  SRR19973063.3768707/1‑150 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           >  SRR19973063.844021/1‑150 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           >  SRR19973063.1824685/1‑150 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           >  SRR19973063.3334542/1‑150 (MQ=60)
                                                                                            ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                           >  SRR19973063.3334536/1‑150 (MQ=60)
                                                                                                 GGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAA                                                      >  SRR19973063.2161233/1‑150 (MQ=60)
                                                                                                  GCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAAC                                                     <  SRR19973063.4975172/150‑1 (MQ=60)
                                                                                                   CTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACA                                                    >  SRR19973063.1453915/1‑150 (MQ=60)
                                                                                                    TATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAC                                                   >  SRR19973063.4820128/1‑150 (MQ=60)
                                                                                                    TATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAC                                                   >  SRR19973063.5142456/1‑150 (MQ=60)
                                                                                                    TATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAC                                                   >  SRR19973063.1689622/1‑150 (MQ=60)
                                                                                                     ATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACC                                                  <  SRR19973063.3302219/150‑1 (MQ=60)
                                                                                                         CCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATG                                              <  SRR19973063.3052872/150‑1 (MQ=60)
                                                                                                          CTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGG                                             >  SRR19973063.1593413/1‑150 (MQ=60)
                                                                                                               AGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATT                                        >  SRR19973063.4638877/1‑150 (MQ=60)
                                                                                                                 GCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTC                                      <  SRR19973063.4341539/150‑1 (MQ=60)
                                                                                                                  CTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCG                                     <  SRR19973063.827856/150‑1 (MQ=60)
                                                                                                                     ATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTCAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATC                                  >  SRR19973063.1600430/1‑150 (MQ=60)
                                                                                                                     ATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATC                                  <  SRR19973063.1606518/150‑1 (MQ=60)
                                                                                                                      TGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCA                                 >  SRR19973063.2056111/1‑150 (MQ=60)
                                                                                                                       GCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAG                                <  SRR19973063.4496245/150‑1 (MQ=60)
                                                                                                                        CAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGT                               >  SRR19973063.3847326/1‑150 (MQ=60)
                                                                                                                          GCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCC                             >  SRR19973063.2944072/1‑150 (MQ=60)
                                                                                                                             AACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTT                          >  SRR19973063.3821067/1‑150 (MQ=60)
                                                                                                                               CTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAA                        <  SRR19973063.2264977/150‑1 (MQ=60)
                                                                                                                                TATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTCGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAAC                       <  SRR19973063.3904912/150‑1 (MQ=60)
                                                                                                                                 ATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGTCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACG                      <  SRR19973063.597035/150‑1 (MQ=60)
                                                                                                                                 ATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACG                      <  SRR19973063.1284937/150‑1 (MQ=60)
                                                                                                                                      ACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAT                 <  SRR19973063.1810198/150‑1 (MQ=60)
                                                                                                                                       CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA                >  SRR19973063.2931183/1‑150 (MQ=60)
                                                                                                                                         TGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACC              <  SRR19973063.4852458/150‑1 (MQ=60)
                                                                                                                                           GACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGT            <  SRR19973063.844482/150‑1 (MQ=60)
                                                                                                                                            ACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTT           <  SRR19973063.4414972/150‑1 (MQ=60)
                                                                                                                                               TATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC        <  SRR19973063.4987445/150‑1 (MQ=60)
                                                                                                                                                   TCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCA    <  SRR19973063.2669949/150‑1 (MQ=60)
                                                                                                                                                    CTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCAC   >  SRR19973063.453534/1‑150 (MQ=60)
                                                                                                                                                     TATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  SRR19973063.2476533/1‑150 (MQ=60)
                                                                                                                                                     TATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  <  SRR19973063.539632/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCACG  >  CP009273/385666‑385964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑