Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,815 G→T 37.4% pseudogene (109/1458 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,8150GT37.4% 427.0 / inf 317pseudogene (109/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base G (105/93);  new base T (64/55);  total (169/148)
Fisher's exact test for biased strand distribution p-value = 9.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 319 total aligned reads displayed.

TGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCAC  >  CP009273/385667‑385963
                                                                                                                                                    |                                                                                                                                                    
tGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGa                                                                                                                                                     >  2:3607370/1‑150 (MQ=255)
 gATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTAt                                                                                                                                                    >  2:4693097/1‑150 (MQ=255)
  aTGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCtata                                                                                                                                                   >  1:4630738/1‑150 (MQ=255)
  aTGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATa                                                                                                                                                   >  2:4268696/1‑150 (MQ=255)
   tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATaa                                                                                                                                                  <  1:5120765/150‑1 (MQ=255)
   tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATaa                                                                                                                                                  <  1:2165440/150‑1 (MQ=255)
    ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAAt                                                                                                                                                 >  2:3025154/1‑150 (MQ=255)
    ggATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAAt                                                                                                                                                 >  2:859381/1‑150 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                             >  2:599434/1‑150 (MQ=255)
        cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                             <  1:4326745/150‑1 (MQ=255)
          aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGtt                                                                                                                                           >  1:3432642/1‑150 (MQ=255)
          aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGtt                                                                                                                                           <  1:3928350/150‑1 (MQ=255)
            ttattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTAc                                                                                                                                         >  1:4661640/1‑150 (MQ=255)
              attaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAg                                                                                                                                       >  1:3059068/1‑150 (MQ=255)
              attaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAg                                                                                                                                       <  1:2551050/150‑1 (MQ=255)
                  cGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACt                                                                                                                                   >  1:4542844/1‑150 (MQ=255)
                   gTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACtt                                                                                                                                  <  2:3445924/150‑1 (MQ=255)
                   gTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACtt                                                                                                                                  >  1:735605/1‑150 (MQ=255)
                     cccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGa                                                                                                                                <  1:1434285/150‑1 (MQ=255)
                           ggaggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGa                                                                                                                          >  2:3883621/1‑150 (MQ=255)
                             aggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATc                                                                                                                        <  1:1606092/150‑1 (MQ=255)
                              gggATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCa                                                                                                                       >  1:3459251/1‑150 (MQ=255)
                                 aTGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCatat                                                                                                                    <  2:902694/150‑1 (MQ=255)
                                  tGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATg                                                                                                                   <  2:2720162/150‑1 (MQ=255)
                                   gACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATgg                                                                                                                  >  1:2581575/1‑150 (MQ=255)
                                           cACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggca                                                                                                          <  1:2889637/150‑1 (MQ=255)
                                            aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTAGATTGGGATCATATGGATAaggcag                                                                                                         >  2:2885613/1‑150 (MQ=255)
                                            aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcag                                                                                                         <  2:3877468/150‑1 (MQ=255)
                                                cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAc                                                                                                     >  2:3331641/1‑150 (MQ=255)
                                                 tGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACt                                                                                                    <  1:3833560/150‑1 (MQ=255)
                                                  ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                                   >  2:2721199/1‑150 (MQ=255)
                                                  ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                                   <  2:614362/150‑1 (MQ=255)
                                                  ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTg                                                                                                   <  2:4923407/150‑1 (MQ=255)
                                                   gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                                  <  1:1027823/150‑1 (MQ=255)
                                                   gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGAt                                                                                                                >  2:1388666/1‑136 (MQ=255)
                                                   gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                                  >  2:2346862/1‑150 (MQ=255)
                                                   gAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                                  <  1:448074/150‑1 (MQ=255)
                                                    aaaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTa                                                                                                 >  2:3703828/1‑150 (MQ=255)
                                                     aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAg                                                                                                <  1:3114418/150‑1 (MQ=255)
                                                       aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                              <  2:440892/150‑1 (MQ=255)
                                                          aaaCTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGc                                                                                           >  2:4536418/1‑150 (MQ=255)
                                                           aaCTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCa                                                                                          <  2:1604252/150‑1 (MQ=255)
                                                           aaCTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCa                                                                                          >  2:1183690/1‑150 (MQ=255)
                                                                 tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                    <  2:1640380/150‑1 (MQ=255)
                                                                 tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                    >  2:3121001/1‑150 (MQ=255)
                                                                  agtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGt                                                                                   >  1:4266095/1‑150 (MQ=255)
                                                                    taTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                                 <  1:3344521/150‑1 (MQ=255)
                                                                      tCACACTTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                               <  2:1442338/150‑1 (MQ=255)
                                                                      tCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                               >  2:274884/1‑150 (MQ=255)
                                                                      tCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                               >  2:383917/1‑150 (MQ=255)
                                                                        acaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACta                                                                             >  1:4794679/1‑150 (MQ=255)
                                                                        acaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACta                                                                             <  2:3562945/150‑1 (MQ=255)
                                                                           aTTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAg                                                                          >  2:5052094/1‑150 (MQ=255)
                                                                           aTTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAg                                                                          >  2:2509075/1‑150 (MQ=255)
                                                                           aTTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAg                                                                          >  2:1962032/1‑150 (MQ=255)
                                                                              aGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTgg                                                                       >  1:3227026/1‑150 (MQ=255)
                                                                              aGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTgg                                                                       >  2:4918287/1‑150 (MQ=255)
                                                                                cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                     >  2:3960747/1‑150 (MQ=255)
                                                                                cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                     >  2:1135897/1‑150 (MQ=255)
                                                                                 aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                    <  2:2202945/150‑1 (MQ=255)
                                                                                 aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                    >  2:456911/1‑150 (MQ=255)
                                                                                   tGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTg                                                                  <  2:51649/150‑1 (MQ=255)
                                                                                     gCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTc                                                                <  2:226448/150‑1 (MQ=255)
                                                                                          cACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTa                                                           <  1:1871236/150‑1 (MQ=255)
                                                                                           aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                          >  2:1874370/1‑150 (MQ=255)
                                                                                            ctctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTaca                                                         <  1:3999412/150‑1 (MQ=255)
                                                                                             tctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaa                                                        >  1:1155478/1‑150 (MQ=255)
                                                                                             tctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaa                                                        <  1:951228/150‑1 (MQ=255)
                                                                                                ggCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaa                                                     <  1:798580/150‑1 (MQ=255)
                                                                                                   tATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAacac                                                  >  2:2456112/1‑150 (MQ=255)
                                                                                                     tCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCa                                                <  1:1564751/150‑1 (MQ=255)
                                                                                                      cACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCaa                                               >  2:1712445/1‑150 (MQ=255)
                                                                                                      cACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCaa                                               >  1:415590/1‑150 (MQ=255)
                                                                                                       aCCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAAt                                              >  1:496207/1‑150 (MQ=255)
                                                                                                       aCCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAAt                                              <  1:1375040/150‑1 (MQ=255)
                                                                                                        ccTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATg                                             >  1:2441346/1‑150 (MQ=255)
                                                                                                           ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTg                                          >  2:2480966/1‑150 (MQ=255)
                                                                                                           ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTg                                          <  1:2412076/150‑1 (MQ=255)
                                                                                                             cAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAt                                        >  1:1359686/1‑150 (MQ=255)
                                                                                                             cAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAt                                        <  2:1813224/150‑1 (MQ=255)
                                                                                                                  tAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGa                                   >  2:3203408/1‑150 (MQ=255)
                                                                                                                     tGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                                <  1:48290/150‑1 (MQ=255)
                                                                                                                           aaaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCtt                          >  1:1296489/1‑150 (MQ=255)
                                                                                                                            aaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCttt                         <  1:5038471/150‑1 (MQ=255)
                                                                                                                               tATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAAc                      <  1:3703767/150‑1 (MQ=255)
                                                                                                                                aTGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                     <  2:4630950/150‑1 (MQ=255)
                                                                                                                                  gATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGc                   <  1:585687/150‑1 (MQ=255)
                                                                                                                                  gATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGc                   <  2:666832/150‑1 (MQ=255)
                                                                                                                                   aTACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg                  <  2:2671821/150‑1 (MQ=255)
                                                                                                                                   aTACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg                  >  2:4201412/1‑150 (MQ=255)
                                                                                                                                   aTACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg                  <  1:2038867/150‑1 (MQ=255)
                                                                                                                                     aCCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAt                <  2:1746004/150‑1 (MQ=255)
                                                                                                                                     aCCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGAt                >  2:3982880/1‑150 (MQ=255)
                                                                                                                                       cTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAc              <  2:571385/150‑1 (MQ=255)
                                                                                                                                           aCTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGtt          >  2:129824/1‑150 (MQ=255)
                                                                                                                                             ttATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa        >  1:2786248/1‑150 (MQ=255)
                                                                                                                                              tataTCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAc       >  1:3248250/1‑150 (MQ=255)
                                                                                                                                               atatCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACg      <  1:407452/150‑1 (MQ=255)
                                                                                                                                                 atCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGc    >  2:3374346/1‑150 (MQ=255)
                                                                                                                                                 atCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGc    <  1:2341712/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCAC  >  CP009273/385667‑385963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A