Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,815 G→T 100% pseudogene (109/1458 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,8150GT98.4% 834.0 / 3.1 253pseudogene (109/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (139/110);  total (141/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 257 total aligned reads displayed.

TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG  >  CP009273/385670‑385960
                                                                                                                                                 |                                                                                                                                                 
tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCtata                                                                                                                                                >  1:1202927/1‑149 (MQ=255)
tGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCtata                                                                                                                                                >  2:1773295/1‑149 (MQ=255)
    tCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATcc                                                                                                                                            >  1:1055132/1‑149 (MQ=255)
     cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                           >  2:4484329/1‑149 (MQ=255)
     cAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATccc                                                                                                                                           >  2:4308326/1‑149 (MQ=255)
      aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                          >  1:4654203/1‑149 (MQ=255)
      aaCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCg                                                                                                                                          <  2:1702017/149‑1 (MQ=255)
       aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGt                                                                                                                                         <  1:1595692/149‑1 (MQ=255)
       aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGt                                                                                                                                         >  2:3130437/1‑149 (MQ=255)
        cTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGtt                                                                                                                                        <  2:4273515/149‑1 (MQ=255)
         ttattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTa                                                                                                                                       >  1:4764851/1‑149 (MQ=255)
         ttattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTa                                                                                                                                       >  1:1303410/1‑149 (MQ=255)
          tattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTAc                                                                                                                                      >  1:3467318/1‑149 (MQ=255)
            ttaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAg                                                                                                                                    >  2:4222070/1‑149 (MQ=255)
               cGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCAc                                                                                                                                 <  2:4788434/149‑1 (MQ=255)
                gTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACt                                                                                                                                <  1:4195906/149‑1 (MQ=255)
                  cccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTg                                                                                                                              <  2:392408/149‑1 (MQ=255)
                  cccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTg                                                                                                                              <  2:3823841/149‑1 (MQ=255)
                   ccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGa                                                                                                                             >  2:3576932/1‑149 (MQ=255)
                   ccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGa                                                                                                                             >  1:103865/1‑149 (MQ=255)
                      gaggagGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTg                                                                                                                          >  2:1472239/1‑149 (MQ=255)
                       aggaggGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTgg                                                                                                                         <  2:1449810/149‑1 (MQ=255)
                            ggATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCa                                                                                                                    <  1:2856939/149‑1 (MQ=255)
                             gATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCat                                                                                                                   >  1:2222362/1‑149 (MQ=255)
                                   aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGAt                                                                                                             <  2:472489/149‑1 (MQ=255)
                                    aaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                            >  2:3999021/1‑149 (MQ=255)
                                        cACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggc                                                                                                        <  1:3832533/149‑1 (MQ=255)
                                         aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggca                                                                                                       >  1:1928074/1‑149 (MQ=255)
                                         aCTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggca                                                                                                       >  1:1046704/1‑149 (MQ=255)
                                            ccTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggcaggc                                                                                                    >  1:574125/1‑149 (MQ=255)
                                             cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAggcaggca                                                                                                   <  1:589997/149‑1 (MQ=255)
                                             cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAggcaggca                                                                                                   >  2:758522/1‑149 (MQ=255)
                                             cTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAggcaggca                                                                                                   >  1:3478161/1‑149 (MQ=255)
                                              tGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAc                                                                                                  <  1:939443/149‑1 (MQ=255)
                                               ggAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACt                                                                                                 >  2:2245050/1‑149 (MQ=255)
                                                 aaaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGt                                                                                               <  1:3990602/149‑1 (MQ=255)
                                                  aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTa                                                                                              >  1:353010/1‑149 (MQ=255)
                                                   aaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGAACTGTAg                                                                                             >  2:468824/1‑149 (MQ=255)
                                                    aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGa                                                                                            >  2:2021833/1‑149 (MQ=255)
                                                     aGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                           >  2:1707476/1‑149 (MQ=255)
                                                        aaCTTGGAGTATCACAATTAGCAGTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACGGTAGATGGc                                                                                        <  2:1391307/149‑1 (MQ=255)
                                                           ttGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAc                                                                                     <  1:3154345/149‑1 (MQ=255)
                                                           ttGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAc                                                                                     <  2:1170071/149‑1 (MQ=255)
                                                              tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTAt                                                                                  <  1:1270523/149‑1 (MQ=255)
                                                                  aTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                              <  1:2562171/149‑1 (MQ=255)
                                                                  aTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                              >  2:2466281/1‑149 (MQ=255)
                                                                  aTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                              >  2:1125854/1‑149 (MQ=255)
                                                                     acaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                           <  1:3311279/149‑1 (MQ=255)
                                                                     acaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                           >  1:2841653/1‑149 (MQ=255)
                                                                     acaATTAGCATTGGCCTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                           <  1:3037090/149‑1 (MQ=255)
                                                                           aGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTg                                                                     >  2:2475785/1‑149 (MQ=255)
                                                                            gCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTgg                                                                    >  1:4450982/1‑149 (MQ=255)
                                                                             cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGt                                                                   >  1:4286330/1‑149 (MQ=255)
                                                                             cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGt                                                                   >  1:2178116/1‑149 (MQ=255)
                                                                              aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                  >  1:2990749/1‑149 (MQ=255)
                                                                               ttGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCACACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                 <  2:1631121/149‑1 (MQ=255)
                                                                               ttGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                 <  2:209315/149‑1 (MQ=255)
                                                                               ttGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                 >  1:2380870/1‑149 (MQ=255)
                                                                                tGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtttt                                                                <  1:2525872/149‑1 (MQ=255)
                                                                                    ttGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCt                                                            >  1:1996468/1‑149 (MQ=255)
                                                                                        aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTa                                                        >  1:713185/1‑149 (MQ=255)
                                                                                         ctctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                       >  1:4648691/1‑149 (MQ=255)
                                                                                          tctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATTATAACTGGACTTATATCTATAATCCCGTTACATCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAATATGTAAAATATATTGGTTTTTTCTATTaaa                                                      >  1:2948746/1‑147 (MQ=255)
                                                                                            tGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaac                                                    >  1:170516/1‑149 (MQ=255)
                                                                                             ggCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaaca                                                   >  1:4710647/1‑149 (MQ=255)
                                                                                              gCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaa                                                  >  2:1059337/1‑149 (MQ=255)
                                                                                               cTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaac                                                 <  2:2834929/149‑1 (MQ=255)
                                                                                                  tCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAcc                                              >  1:1466686/1‑149 (MQ=255)
                                                                                                      ctctCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATg                                          <  2:4786718/149‑1 (MQ=255)
                                                                                                       tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                         <  2:574125/149‑1 (MQ=255)
                                                                                                       tctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                         <  1:2321087/149‑1 (MQ=255)
                                                                                                        ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGt                                        <  2:3227170/149‑1 (MQ=255)
                                                                                                              cTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTc                                  <  1:956873/149‑1 (MQ=255)
                                                                                                                  tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGAta                              >  2:4611998/1‑148 (MQ=255)
                                                                                                                  tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATc                              >  2:1918693/1‑149 (MQ=255)
                                                                                                                  tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATc                              >  1:1716902/1‑149 (MQ=255)
                                                                                                                   gcagcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                             >  1:3278451/1‑149 (MQ=255)
                                                                                                                    cagcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAg                            >  2:1025333/1‑149 (MQ=255)
                                                                                                                      gcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                          >  2:2134919/1‑149 (MQ=255)
                                                                                                                         aaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCtt                       <  2:1988589/149‑1 (MQ=255)
                                                                                                                          aCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCttt                      <  2:3682693/149‑1 (MQ=255)
                                                                                                                          aCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCttt                      <  2:2722180/149‑1 (MQ=255)
                                                                                                                           cTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTa                     <  1:267035/149‑1 (MQ=255)
                                                                                                                             aTGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAAc                   >  2:3562418/1‑149 (MQ=255)
                                                                                                                              tGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACg                  >  2:1211637/1‑149 (MQ=255)
                                                                                                                                  aCCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGa              <  2:368417/149‑1 (MQ=255)
                                                                                                                                  aCCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGa              <  2:3043025/149‑1 (MQ=255)
                                                                                                                                    cTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATa            >  1:1677887/1‑149 (MQ=255)
                                                                                                                                    cTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTAGTACAACAACACCAATGGTGATTTCGATCAGTCCTGTAACGGCGATa            >  2:1574707/1‑149 (MQ=255)
                                                                                                                                    cTGGAATTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATa            <  1:3130437/149‑1 (MQ=255)
                                                                                                                                     tGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAc           <  2:339679/149‑1 (MQ=255)
                                                                                                                                     tGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAc           <  2:1605865/149‑1 (MQ=255)
                                                                                                                                      ggACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAcc          >  2:3841220/1‑149 (MQ=255)
                                                                                                                                       gACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCg         >  2:1227736/1‑149 (MQ=255)
                                                                                                                                       gACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCg         >  2:587666/1‑149 (MQ=255)
                                                                                                                                        aCTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGt        >  1:855268/1‑149 (MQ=255)
                                                                                                                                         cTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGtt       <  2:4105966/149‑1 (MQ=255)
                                                                                                                                           tataTCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa     >  2:2202057/1‑149 (MQ=255)
                                                                                                                                            atatCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAc    >  1:4421610/1‑149 (MQ=255)
                                                                                                                                             tatCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACg   <  1:4126605/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG  >  CP009273/385670‑385960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGC  >  CP009273/385669‑385961
                                                                                                                                                  |                                                                                                                                                  
ATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATA                                                                                                                                                 >  SRR19973071.1207026/1‑150 (MQ=60)
ATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATA                                                                                                                                                 >  SRR19973071.461266/1‑150 (MQ=60)
 TGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAA                                                                                                                                                >  SRR19973071.4120567/1‑150 (MQ=60)
   GATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATC                                                                                                                                              >  SRR19973071.3041602/1‑150 (MQ=60)
    ATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCC                                                                                                                                             >  SRR19973071.1058915/1‑150 (MQ=60)
      CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCG                                                                                                                                           >  SRR19973071.4670732/1‑150 (MQ=60)
      CAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCG                                                                                                                                           >  SRR19973071.4670776/1‑150 (MQ=60)
        ACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTT                                                                                                                                         <  SRR19973071.1601325/150‑1 (MQ=60)
         CTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTA                                                                                                                                        >  SRR19973071.1308165/1‑150 (MQ=60)
         CTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTA                                                                                                                                        >  SRR19973071.4781684/1‑150 (MQ=60)
          TTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTAC                                                                                                                                       >  SRR19973071.3479543/1‑150 (MQ=60)
                 GTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTT                                                                                                                                <  SRR19973071.4210846/150‑1 (MQ=60)
                   CCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGA                                                                                                                              >  SRR19973071.104409/1‑150 (MQ=60)
                   CCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGA                                                                                                                              >  SRR19973071.104388/1‑150 (MQ=60)
                   CCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGA                                                                                                                              >  SRR19973071.3527993/1‑150 (MQ=60)
                    CCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAT                                                                                                                             >  SRR19973071.3053844/1‑150 (MQ=60)
                      TGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTG                                                                                                                           <  SRR19973071.2739558/150‑1 (MQ=60)
                       GAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGG                                                                                                                          >  SRR19973071.1314699/1‑150 (MQ=60)
                       GAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGG                                                                                                                          <  SRR19973071.1781602/150‑1 (MQ=60)
                          GAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGAT                                                                                                                       >  SRR19973071.3477519/1‑150 (MQ=60)
                             GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCAT                                                                                                                    <  SRR19973071.2867103/150‑1 (MQ=60)
                             GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCAT                                                                                                                    >  SRR19973071.2230570/1‑150 (MQ=60)
                             GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCAT                                                                                                                    <  SRR19973071.4323562/150‑1 (MQ=60)
                             GGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAGTGGGATCAT                                                                                                                    >  SRR19973071.2230586/1‑150 (MQ=60)
                              GATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATA                                                                                                                   <  SRR19973071.2990682/150‑1 (MQ=60)
                              GATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATA                                                                                                                   >  SRR19973071.835771/1‑150 (MQ=60)
                                TGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCACACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATG                                                                                                                 <  SRR19973071.2902413/150‑1 (MQ=60)
                                  ACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGA                                                                                                               >  SRR19973071.453262/1‑150 (MQ=60)
                                         CACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCA                                                                                                        >  SRR19973071.1050467/1‑150 (MQ=60)
                                         CACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCA                                                                                                        >  SRR19973071.1935106/1‑150 (MQ=60)
                                         CACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCA                                                                                                        <  SRR19973071.3846117/150‑1 (MQ=60)
                                          ACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAG                                                                                                       <  SRR19973071.2864815/150‑1 (MQ=60)
                                            TCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGC                                                                                                     >  SRR19973071.576277/1‑150 (MQ=60)
                                            TCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGC                                                                                                     <  SRR19973071.2354413/150‑1 (MQ=60)
                                             CCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCA                                                                                                    >  SRR19973071.3490411/1‑150 (MQ=60)
                                             CCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCA                                                                                                    >  SRR19973071.3490381/1‑150 (MQ=60)
                                              CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                   <  SRR19973071.592172/150‑1 (MQ=60)
                                              CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                   >  SRR19973071.1862217/1‑150 (MQ=60)
                                              CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                   <  SRR19973071.592196/150‑1 (MQ=60)
                                              CTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCAC                                                                                                   <  SRR19973071.591869/150‑1 (MQ=60)
                                               TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACT                                                                                                  <  SRR19973071.942863/150‑1 (MQ=60)
                                               TGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACT                                                                                                  <  SRR19973071.942861/150‑1 (MQ=60)
                                                GGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTG                                                                                                 >  SRR19973071.2764335/1‑150 (MQ=60)
                                                  AAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTA                                                                                               >  SRR19973071.354162/1‑150 (MQ=60)
                                                  AAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTA                                                                                               <  SRR19973071.4004549/150‑1 (MQ=60)
                                                    AAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCATGCACTGTAGA                                                                                             <  SRR19973071.952055/150‑1 (MQ=60)
                                                    AAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGA                                                                                             >  SRR19973071.4284414/1‑150 (MQ=60)
                                                       GAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGG                                                                                          <  SRR19973071.201797/150‑1 (MQ=60)
                                                         AACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCA                                                                                        >  SRR19973071.4701904/1‑150 (MQ=60)
                                                          ACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAA                                                                                       >  SRR19973071.2183057/1‑150 (MQ=60)
                                                            TTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACT                                                                                     <  SRR19973071.3165378/150‑1 (MQ=60)
                                                            TTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACT                                                                                     <  SRR19973071.3165247/150‑1 (MQ=60)
                                                             TGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTA                                                                                    >  SRR19973071.1557252/1‑150 (MQ=60)
                                                              GTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTAT                                                                                   >  SRR19973071.3898553/1‑150 (MQ=60)
                                                               TAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATG                                                                                  <  SRR19973071.1275116/150‑1 (MQ=60)
                                                                   ATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAA                                                                              <  SRR19973071.2571471/150‑1 (MQ=60)
                                                                     CACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACT                                                                            <  SRR19973071.3097490/150‑1 (MQ=60)
                                                                     CACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACT                                                                            >  SRR19973071.2851756/1‑150 (MQ=60)
                                                                     CACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAGTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACT                                                                            >  SRR19973071.1659774/1‑150 (MQ=60)
                                                                      ACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTA                                                                           <  SRR19973071.3323019/150‑1 (MQ=60)
                                                                      ACAATTAGCATTGGCCTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTA                                                                           <  SRR19973071.3047892/150‑1 (MQ=60)
                                                                          TTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGT                                                                       >  SRR19973071.2336319/1‑150 (MQ=60)
                                                                            AGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGG                                                                     >  SRR19973071.1629470/1‑150 (MQ=60)
                                                                            AGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGG                                                                     >  SRR19973071.4466729/1‑150 (MQ=60)
                                                                            AGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGG                                                                     >  SRR19973071.4466465/1‑150 (MQ=60)
                                                                             GCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGT                                                                    >  SRR19973071.4301500/1‑150 (MQ=60)
                                                                             GCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGT                                                                    <  SRR19973071.408958/150‑1 (MQ=60)
                                                                             GCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGT                                                                    >  SRR19973071.2186170/1‑150 (MQ=60)
                                                                              CATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTT                                                                   >  SRR19973071.3001447/1‑150 (MQ=60)
                                                                               ATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTT                                                                  >  SRR19973071.2389452/1‑150 (MQ=60)
                                                                                 TGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTG                                                                >  SRR19973071.1719326/1‑150 (MQ=60)
                                                                                 TGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTG                                                                <  SRR19973071.2535062/150‑1 (MQ=60)
                                                                                  GGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGT                                                               >  SRR19973071.1280249/1‑150 (MQ=60)
                                                                                  GGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGT                                                               <  SRR19973071.1686821/150‑1 (MQ=60)
                                                                                    CTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCT                                                             <  SRR19973071.332537/150‑1 (MQ=60)
                                                                                    CTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCT                                                             >  SRR19973071.2003664/1‑150 (MQ=60)
                                                                                    CTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCT                                                             <  SRR19973071.2612616/150‑1 (MQ=60)
                                                                                      TGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTAT                                                           >  SRR19973071.4377464/1‑150 (MQ=60)
                                                                                        CACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTA                                                         >  SRR19973071.715765/1‑150 (MQ=60)
                                                                                        CACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTA                                                         >  SRR19973071.715679/1‑150 (MQ=60)
                                                                                         ACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTAC                                                        >  SRR19973071.4665246/1‑150 (MQ=60)
                                                                                          CTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATTATAACTGGACTTATATCTATAATCCCGTTACATCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAATATGTAAAATATATTGGTTTTTTCTATTAAA                                                       >  SRR19973071.2959308/1‑150 (MQ=60)
                                                                                          CTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACA                                                       >  SRR19973071.2959040/1‑150 (MQ=60)
                                                                                            CTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAAC                                                     >  SRR19973071.1137827/1‑150 (MQ=60)
                                                                                            CTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAAC                                                     >  SRR19973071.171243/1‑150 (MQ=60)
                                                                                             TGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACA                                                    >  SRR19973071.4727355/1‑150 (MQ=60)
                                                                                             TGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACA                                                    >  SRR19973071.4692621/1‑150 (MQ=60)
                                                                                              GGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAA                                                   >  SRR19973071.525477/1‑150 (MQ=60)
                                                                                              GGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAA                                                   >  SRR19973071.525383/1‑150 (MQ=60)
                                                                                                 TATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAC                                                >  SRR19973071.360895/1‑150 (MQ=60)
                                                                                                  ATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACC                                               >  SRR19973071.1472031/1‑150 (MQ=60)
                                                                                                   TCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCA                                              <  SRR19973071.1241019/150‑1 (MQ=60)
                                                                                                       CTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGG                                          <  SRR19973071.1211378/150‑1 (MQ=60)
                                                                                                        TCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGT                                         <  SRR19973071.2329539/150‑1 (MQ=60)
                                                                                                           CAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAT                                      <  SRR19973071.875909/150‑1 (MQ=60)
                                                                                                           CAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAT                                      <  SRR19973071.4214625/150‑1 (MQ=60)
                                                                                                            AGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATT                                     <  SRR19973071.1400454/150‑1 (MQ=60)
                                                                                                             GGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTT                                    <  SRR19973071.1983522/150‑1 (MQ=60)
                                                                                                               CTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCG                                  <  SRR19973071.960349/150‑1 (MQ=60)
                                                                                                                 AATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGAT                                <  SRR19973071.2441988/150‑1 (MQ=60)
                                                                                                                  ATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATC                               >  SRR19973071.1723282/1‑150 (MQ=60)
                                                                                                                   TGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCA                              >  SRR19973071.3288514/1‑150 (MQ=60)
                                                                                                                   TGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCA                              >  SRR19973071.3290310/1‑150 (MQ=60)
                                                                                                                   TGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCA                              >  SRR19973071.3290056/1‑150 (MQ=60)
                                                                                                                            CTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAA                     <  SRR19973071.268024/150‑1 (MQ=60)
                                                                                                                              ATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACG                   <  SRR19973071.1053655/150‑1 (MQ=60)
                                                                                                                                  TACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGA               >  SRR19973071.2970619/1‑150 (MQ=60)
                                                                                                                                    CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA             >  SRR19973071.1684065/1‑150 (MQ=60)
                                                                                                                                    CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA             >  SRR19973071.1684101/1‑150 (MQ=60)
                                                                                                                                    CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA             >  SRR19973071.1052579/1‑150 (MQ=60)
                                                                                                                                    CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA             >  SRR19973071.2538794/1‑150 (MQ=60)
                                                                                                                                    CCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATA             >  SRR19973071.2538380/1‑150 (MQ=60)
                                                                                                                                     CTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAC            >  SRR19973071.999481/1‑150 (MQ=60)
                                                                                                                                     CTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAC            >  SRR19973071.2255206/1‑150 (MQ=60)
                                                                                                                                     CTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAC            >  SRR19973071.3489176/1‑150 (MQ=60)
                                                                                                                                     CTGGAATTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATAC            <  SRR19973071.3141436/150‑1 (MQ=60)
                                                                                                                                       GGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCG          >  SRR19973071.2194693/1‑150 (MQ=60)
                                                                                                                                        GACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGT         >  SRR19973071.858318/1‑150 (MQ=60)
                                                                                                                                         ACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTT        >  SRR19973071.3813710/1‑150 (MQ=60)
                                                                                                                                            TATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAC     >  SRR19973071.4437201/1‑150 (MQ=60)
                                                                                                                                             ATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACG    <  SRR19973071.618033/150‑1 (MQ=60)
                                                                                                                                             ATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACG    >  SRR19973071.3275414/1‑150 (MQ=60)
                                                                                                                                              TATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG   <  SRR19973071.4730513/150‑1 (MQ=60)
                                                                                                                                              TATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG   <  SRR19973071.4141294/150‑1 (MQ=60)
                                                                                                                                              TATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGG   <  SRR19973071.4142676/150‑1 (MQ=60)
                                                                                                                                               ATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGC  >  SRR19973071.115058/1‑150 (MQ=60)
                                                                                                                                               ATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGC  >  SRR19973071.2260084/1‑150 (MQ=60)
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ATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGC  >  CP009273/385669‑385961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑