Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,587,029:1 +G 100% coding (101/699 nt) ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,587,0291.G96.9% 1010.7 / 23.2 257coding (101/699 nt)ypfHpalmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base G (134/115);  total (137/120)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTC  >  CP009273/2586880‑2587173
                                                                                                                                                      |                                                                                                                                                
tGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAttt                                                                                                                                                   >  1:3177259/1‑150 (MQ=255)
 gATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAtttg                                                                                                                                                  >  1:281878/1‑149 (MQ=255)
   tCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gcc                                                                                                                                               <  2:3348442/150‑1 (MQ=255)
         ccACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCAtt                                                                                                                                          <  2:678800/150‑1 (MQ=255)
          cacaCGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTg                                                                                                                                         <  2:3848411/150‑1 (MQ=255)
             acGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCa                                                                                                                                      >  1:3700071/1‑150 (MQ=255)
               gcgcCTGGCGATTATCTTCCGTAATACCCTGCACCGAAATCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCAcc                                                                                                                                    <  1:173013/150‑1 (MQ=255)
                cgcCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCg                                                                                                                                   <  1:1074004/150‑1 (MQ=255)
                cgcCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCg                                                                                                                                   >  2:978840/1‑150 (MQ=255)
                cgcCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCg                                                                                                                                   >  2:1470700/1‑150 (MQ=255)
                 gcCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCgg                                                                                                                                  >  2:2964785/1‑150 (MQ=255)
                  ccTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCggg                                                                                                                                 >  1:2289383/1‑150 (MQ=255)
                       cGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATc                                                                                                                            >  2:2151618/1‑150 (MQ=255)
                          ttATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATcccc                                                                                                                         <  2:281878/150‑1 (MQ=255)
                           tATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCg                                                                                                                        >  2:2212019/1‑150 (MQ=255)
                            aTCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGa                                                                                                                       >  2:4031403/1‑150 (MQ=255)
                                  cGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGt                                                                                                                 >  1:3824133/1‑150 (MQ=255)
                                  cGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGt                                                                                                                 >  2:3652657/1‑150 (MQ=255)
                                   gTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTg                                                                                                                <  2:3500230/150‑1 (MQ=255)
                                   gTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTg                                                                                                                >  1:1275741/1‑150 (MQ=255)
                                     aaTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGa                                                                                                              >  2:1676658/1‑150 (MQ=255)
                                     aaTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGa                                                                                                              >  1:4213281/1‑150 (MQ=255)
                                      aTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaa                                                                                                             >  2:4568683/1‑150 (MQ=255)
                                       tACCCTGCACCGAAAACCACTGCCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaaa                                                                                                            <  2:2311089/150‑1 (MQ=255)
                                        aCCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaaaa                                                                                                           >  2:3715752/1‑150 (MQ=255)
                                         cccTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAg                                                                                                          >  1:1249351/1‑150 (MQ=255)
                                         cccTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAg                                                                                                          >  1:1465186/1‑150 (MQ=255)
                                           cTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCa                                                                                                        >  1:4588849/1‑150 (MQ=255)
                                             gCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGt                                                                                                      <  2:1175870/150‑1 (MQ=255)
                                                ccGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaac                                                                                                   <  2:3078016/150‑1 (MQ=255)
                                                ccGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaac                                                                                                   <  1:1515421/150‑1 (MQ=255)
                                                 cGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaaca                                                                                                  >  2:730388/1‑150 (MQ=255)
                                                 cGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaaca                                                                                                  <  2:3728729/150‑1 (MQ=255)
                                                   aaaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaac                                                                                                <  2:1140738/150‑1 (MQ=255)
                                                    aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACt                                                                                               >  2:4203479/1‑150 (MQ=255)
                                                    aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACt                                                                                               <  1:4470015/150‑1 (MQ=255)
                                                    aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACt                                                                                               >  1:3536996/1‑150 (MQ=255)
                                                         aCTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgc                                                                                          >  1:3994591/1‑150 (MQ=255)
                                                         aCTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgc                                                                                          >  1:2067734/1‑150 (MQ=255)
                                                          cTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcg                                                                                         >  1:806525/1‑150 (MQ=255)
                                                          cTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcg                                                                                         <  2:1980250/150‑1 (MQ=255)
                                                             aCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAg                                                                                      >  2:582849/1‑150 (MQ=255)
                                                              cGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAgg                                                                                     >  2:646033/1‑150 (MQ=255)
                                                               gCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGt                                                                                    <  2:443598/150‑1 (MQ=255)
                                                                 cccGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGttt                                                                                  <  1:1734787/150‑1 (MQ=255)
                                                                  ccgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTa                                                                                 >  2:3243212/1‑150 (MQ=255)
                                                                   cgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                                >  1:4365129/1‑150 (MQ=255)
                                                                   cgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                                <  1:578091/150‑1 (MQ=255)
                                                                   cgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                                <  1:922668/150‑1 (MQ=255)
                                                                   cgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                                >  1:1414845/1‑150 (MQ=255)
                                                                    gccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATc                                                                               <  1:1141287/150‑1 (MQ=255)
                                                                    gccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATc                                                                               <  1:3564499/150‑1 (MQ=255)
                                                                     ccgGATAACCGCTCGGCTCCGCGCCACCGACACACACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATcc                                                                              >  2:4253591/1‑150 (MQ=255)
                                                                      cgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCg                                                                             >  1:2412229/1‑150 (MQ=255)
                                                                      cgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCg                                                                             <  2:1441690/150‑1 (MQ=255)
                                                                      cgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCg                                                                             >  1:3106306/1‑150 (MQ=255)
                                                                       ggATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCgg                                                                            >  1:3708500/1‑150 (MQ=255)
                                                                           tACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCtt                                                                        >  1:3593900/1‑150 (MQ=255)
                                                                           tACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCtt                                                                        <  2:1652633/150‑1 (MQ=255)
                                                                            aCCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCttt                                                                       <  2:3065081/150‑1 (MQ=255)
                                                                             ccGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTg                                                                      >  1:965977/1‑150 (MQ=255)
                                                                               gCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaa                                                                    >  2:3748276/1‑150 (MQ=255)
                                                                               gCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaa                                                                    <  1:3002588/150‑1 (MQ=255)
                                                                                 tCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaaca                                                                  <  1:3285787/150‑1 (MQ=255)
                                                                                   ggCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTCTTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaacaac                                                                >  2:1837928/1‑150 (MQ=255)
                                                                                    gCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAacaaca                                                               <  1:4223977/150‑1 (MQ=255)
                                                                                          cgcCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATgg                                                         >  2:4476528/1‑150 (MQ=255)
                                                                                            ccACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTc                                                       >  2:3834930/1‑150 (MQ=255)
                                                                                             cACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCa                                                      <  1:4374896/150‑1 (MQ=255)
                                                                                              aCCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCAt                                                     <  2:1560440/150‑1 (MQ=255)
                                                                                              aCCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCAt                                                     >  2:3397632/1‑150 (MQ=255)
                                                                                                 gACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGtt                                                  <  2:892463/150‑1 (MQ=255)
                                                                                                    acTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCa                                               <  1:3341239/150‑1 (MQ=255)
                                                                                                     cTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCAt                                              >  2:3457197/1‑150 (MQ=255)
                                                                                                       caccacCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATgg                                            >  2:2595041/1‑150 (MQ=255)
                                                                                                       caccacCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATgg                                            <  1:3644713/150‑1 (MQ=255)
                                                                                                        accaccAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGc                                           >  1:3425113/1‑150 (MQ=255)
                                                                                                         ccaccaGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCg                                          <  1:2147254/150‑1 (MQ=255)
                                                                                                            ccaGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCCTTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAAc                                       <  1:2570794/150‑1 (MQ=255)
                                                                                                             caGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACt                                      >  2:1400224/1‑150 (MQ=255)
                                                                                                                 gcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCtt                                  >  1:1570118/1‑150 (MQ=255)
                                                                                                                  cATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTa                                 <  2:464893/150‑1 (MQ=255)
                                                                                                                   aTCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTaa                                >  2:2855788/1‑150 (MQ=255)
                                                                                                                      aGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGTAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTg                             <  1:186675/150‑1 (MQ=255)
                                                                                                                        gAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGAt                           >  2:2835808/1‑150 (MQ=255)
                                                                                                                          aaCAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGt                         <  1:4332273/150‑1 (MQ=255)
                                                                                                                          aaCAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTc                        <  1:2617249/150‑1 (MQ=255)
                                                                                                                            cAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCa                       >  2:3142101/1‑150 (MQ=255)
                                                                                                                               cGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTa                    <  2:102197/150‑1 (MQ=255)
                                                                                                                               cGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTa                    >  1:1962838/1‑150 (MQ=255)
                                                                                                                                ggTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTaa                   >  2:1277800/1‑150 (MQ=255)
                                                                                                                                ggTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTaa                   >  2:3919309/1‑150 (MQ=255)
                                                                                                                                 gTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAt                  >  2:1815714/1‑150 (MQ=255)
                                                                                                                                   gCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAttt                >  1:1006924/1‑150 (MQ=255)
                                                                                                                                   gCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAttt                <  1:3932874/150‑1 (MQ=255)
                                                                                                                                      aaCCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTAc             >  1:2955580/1‑150 (MQ=255)
                                                                                                                                       aCCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTAcg            <  1:1839864/150‑1 (MQ=255)
                                                                                                                                            gtGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCt       <  2:3511004/149‑1 (MQ=255)
                                                                                                                                              gCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGa     >  2:222187/1‑150 (MQ=255)
                                                                                                                                                cTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACt   >  2:2671741/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTC  >  CP009273/2586880‑2587173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  CP009273/2586880‑2587178
                                                                                                                                                     |                                                                                                                                                      
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT                                                                                                                                                        >  SRR19973073.3183664/1‑150 (MQ=60)
 GATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑G                                                                                                                                                      <  SRR19973073.3390158/150‑1 (MQ=60)
 GATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑G                                                                                                                                                      >  SRR19973073.282480/1‑150 (MQ=60)
    CGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑GCCC                                                                                                                                                   <  SRR19973073.153701/150‑1 (MQ=60)
      CATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCC                                                                                                                                                  >  SRR19973073.397123/1‑150 (MQ=60)
       ATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCA                                                                                                                                                 >  SRR19973073.3183089/1‑150 (MQ=60)
            CACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCC                                                                                                                                            >  SRR19973073.1856580/1‑150 (MQ=60)
             ACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCA                                                                                                                                           >  SRR19973073.3708107/1‑150 (MQ=60)
             ACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCA                                                                                                                                           >  SRR19973073.3707498/1‑150 (MQ=60)
             ACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCA                                                                                                                                           <  SRR19973073.2605101/150‑1 (MQ=60)
              CGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCAC                                                                                                                                          <  SRR19973073.4445170/150‑1 (MQ=60)
               GCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAATCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACC                                                                                                                                         <  SRR19973073.173421/150‑1 (MQ=60)
               GCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACC                                                                                                                                         <  SRR19973073.173360/150‑1 (MQ=60)
                CGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCG                                                                                                                                        <  SRR19973073.1076237/150‑1 (MQ=60)
                CGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCG                                                                                                                                        <  SRR19973073.1076223/150‑1 (MQ=60)
                  CCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGG                                                                                                                                      >  SRR19973073.2294057/1‑150 (MQ=60)
                    TGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTT                                                                                                                                    <  SRR19973073.3637551/150‑1 (MQ=60)
                    TGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTT                                                                                                                                    >  SRR19973073.1997208/1‑150 (MQ=60)
                    TGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTT                                                                                                                                    <  SRR19973073.3947471/150‑1 (MQ=60)
                     GGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTA                                                                                                                                   >  SRR19973073.1775795/1‑150 (MQ=60)
                        GATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCC                                                                                                                                >  SRR19973073.3353853/1‑150 (MQ=60)
                        GATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCC                                                                                                                                >  SRR19973073.1983546/1‑150 (MQ=60)
                           TATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCG                                                                                                                             >  SRR19973073.1702916/1‑150 (MQ=60)
                           TATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCG                                                                                                                             >  SRR19973073.3161417/1‑150 (MQ=60)
                           TATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCG                                                                                                                             >  SRR19973073.2513621/1‑150 (MQ=60)
                                 CCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCG                                                                                                                       >  SRR19973073.842167/1‑150 (MQ=60)
                                  CGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGT                                                                                                                      >  SRR19973073.3602616/1‑150 (MQ=60)
                                  CGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGT                                                                                                                      >  SRR19973073.3831742/1‑150 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTG                                                                                                                     >  SRR19973073.1278414/1‑150 (MQ=60)
                                    TAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGG                                                                                                                    >  SRR19973073.3543625/1‑150 (MQ=60)
                                     AATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGA                                                                                                                   >  SRR19973073.4221713/1‑150 (MQ=60)
                                     AATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGA                                                                                                                   >  SRR19973073.4221641/1‑150 (MQ=60)
                                     AATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGA                                                                                                                   >  SRR19973073.4204698/1‑150 (MQ=60)
                                      ATACCCTGCACCGAGAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  <  SRR19973073.1314588/150‑1 (MQ=60)
                                      ATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  <  SRR19973073.1861105/150‑1 (MQ=60)
                                        ACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAA                                                                                                                >  SRR19973073.3077643/1‑150 (MQ=60)
                                         CCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAG                                                                                                               >  SRR19973073.1468254/1‑150 (MQ=60)
                                         CCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAG                                                                                                               >  SRR19973073.1251964/1‑150 (MQ=60)
                                          CCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGC                                                                                                              <  SRR19973073.3804160/150‑1 (MQ=60)
                                           CTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCA                                                                                                             >  SRR19973073.4598020/1‑150 (MQ=60)
                                           CTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCA                                                                                                             >  SRR19973073.358961/1‑150 (MQ=60)
                                            TGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAG                                                                                                            <  SRR19973073.4564336/150‑1 (MQ=60)
                                             GCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGT                                                                                                           >  SRR19973073.3203973/1‑150 (MQ=60)
                                             GCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGT                                                                                                           >  SRR19973073.2298602/1‑150 (MQ=60)
                                             GCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGT                                                                                                           >  SRR19973073.2298611/1‑150 (MQ=60)
                                                CCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAAC                                                                                                        >  SRR19973073.4422391/1‑150 (MQ=60)
                                                CCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAAC                                                                                                        <  SRR19973073.1518544/150‑1 (MQ=60)
                                                    AAACCANTGACGCCCCGCCGGATTNCCGCTCGGNTCCGCGCCANCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    <  SRR19973073.4478943/150‑1 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    <  SRR19973073.4479007/150‑1 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    <  SRR19973073.2839211/150‑1 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    >  SRR19973073.3544078/1‑150 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    >  SRR19973073.3896508/1‑150 (MQ=60)
                                                         ACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGC                                                                                               >  SRR19973073.2072041/1‑150 (MQ=60)
                                                         ACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGC                                                                                               >  SRR19973073.2072040/1‑150 (MQ=60)
                                                         ACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGC                                                                                               >  SRR19973073.872953/1‑150 (MQ=60)
                                                         ACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGC                                                                                               >  SRR19973073.935594/1‑150 (MQ=60)
                                                         ACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGC                                                                                               >  SRR19973073.4002557/1‑150 (MQ=60)
                                                          CTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCG                                                                                              >  SRR19973073.808161/1‑150 (MQ=60)
                                                            GACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCA                                                                                            >  SRR19973073.2076076/1‑150 (MQ=60)
                                                            GACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCA                                                                                            <  SRR19973073.3305179/150‑1 (MQ=60)
                                                                 CCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTT                                                                                       <  SRR19973073.1738384/150‑1 (MQ=60)
                                                                 CCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTT                                                                                       <  SRR19973073.1738392/150‑1 (MQ=60)
                                                                 CCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTT                                                                                       <  SRR19973073.1818207/150‑1 (MQ=60)
                                                                 CCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTT                                                                                       >  SRR19973073.1427786/1‑150 (MQ=60)
                                                                  CCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTA                                                                                      <  SRR19973073.3744904/150‑1 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     >  SRR19973073.4373875/1‑150 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     >  SRR19973073.4373746/1‑150 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     <  SRR19973073.579330/150‑1 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     <  SRR19973073.4282567/150‑1 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     >  SRR19973073.601298/1‑150 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     <  SRR19973073.924587/150‑1 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     >  SRR19973073.1417828/1‑150 (MQ=60)
                                                                    GCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATC                                                                                    <  SRR19973073.3571641/150‑1 (MQ=60)
                                                                    GCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATC                                                                                    >  SRR19973073.1164844/1‑150 (MQ=60)
                                                                    GCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATC                                                                                    <  SRR19973073.1143600/150‑1 (MQ=60)
                                                                     CCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCC                                                                                   <  SRR19973073.4369578/150‑1 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973073.2417197/1‑150 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973073.3112553/1‑150 (MQ=60)
                                                                       GGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGG                                                                                 >  SRR19973073.3715952/1‑150 (MQ=60)
                                                                       GGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGG                                                                                 >  SRR19973073.3715941/1‑150 (MQ=60)
                                                                          TTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCT                                                                              >  SRR19973073.4523305/1‑150 (MQ=60)
                                                                           TACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTT                                                                             >  SRR19973073.3601110/1‑150 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973073.1253303/150‑1 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973073.155140/150‑1 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973073.1953509/150‑1 (MQ=60)
                                                                             CCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTG                                                                           >  SRR19973073.967998/1‑150 (MQ=60)
                                                                               GCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAA                                                                         <  SRR19973073.3008683/150‑1 (MQ=60)
                                                                                 TCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACA                                                                       <  SRR19973073.590609/150‑1 (MQ=60)
                                                                                 TCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACA                                                                       <  SRR19973073.3292443/150‑1 (MQ=60)
                                                                                    GCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACA                                                                    <  SRR19973073.4232352/150‑1 (MQ=60)
                                                                                      TCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAA                                                                  <  SRR19973073.3269072/150‑1 (MQ=60)
                                                                                        CGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAAT                                                                <  SRR19973073.1328840/150‑1 (MQ=60)
                                                                                        CGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAAT                                                                <  SRR19973073.4634521/150‑1 (MQ=60)
                                                                                             CACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCA                                                           <  SRR19973073.4383457/150‑1 (MQ=60)
                                                                                             CACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCA                                                           <  SRR19973073.4383671/150‑1 (MQ=60)
                                                                                                   CACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTC                                                     <  SRR19973073.3575863/150‑1 (MQ=60)
                                                                                                   CACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTC                                                     <  SRR19973073.4062549/150‑1 (MQ=60)
                                                                                                    ACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCA                                                    <  SRR19973073.3347995/150‑1 (MQ=60)
                                                                                                       CACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGG                                                 <  SRR19973073.3652039/150‑1 (MQ=60)
                                                                                                       CACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGG                                                 <  SRR19973073.3652027/150‑1 (MQ=60)
                                                                                                        ACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGC                                                >  SRR19973073.3431957/1‑150 (MQ=60)
                                                                                                        ACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGC                                                >  SRR19973073.3432040/1‑150 (MQ=60)
                                                                                                         CCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCG                                               <  SRR19973073.3571586/150‑1 (MQ=60)
                                                                                                         CCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCG                                               <  SRR19973073.2151745/150‑1 (MQ=60)
                                                                                                         CCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCG                                               <  SRR19973073.284496/150‑1 (MQ=60)
                                                                                                            CCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCCTTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAAC                                            <  SRR19973073.2576092/150‑1 (MQ=60)
                                                                                                            CCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAAC                                            >  SRR19973073.2163058/1‑150 (MQ=60)
                                                                                                            CCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAAC                                            <  SRR19973073.2576073/150‑1 (MQ=60)
                                                                                                             CAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTGGAACACCAAAATGGTCATGTTTCATGGCGAACT                                           >  SRR19973073.4279354/1‑150 (MQ=60)
                                                                                                                CGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCT                                        <  SRR19973073.2972547/150‑1 (MQ=60)
                                                                                                                 GCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTT                                       >  SRR19973073.1573346/1‑150 (MQ=60)
                                                                                                                   ATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAA                                     >  SRR19973073.3097411/1‑150 (MQ=60)
                                                                                                                     CAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACT                                   <  SRR19973073.1445642/150‑1 (MQ=60)
                                                                                                                     CAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACT                                   >  SRR19973073.1114916/1‑150 (MQ=60)
                                                                                                                      AGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGTAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTG                                  <  SRR19973073.187115/150‑1 (MQ=60)
                                                                                                                          AACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGT                              <  SRR19973073.4340931/150‑1 (MQ=60)
                                                                                                                          AACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGT                              <  SRR19973073.2696532/150‑1 (MQ=60)
                                                                                                                          AACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTC                             <  SRR19973073.2622631/150‑1 (MQ=60)
                                                                                                                             AGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCAT                           >  SRR19973073.2663619/1‑150 (MQ=60)
                                                                                                                               CGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTA                         >  SRR19973073.1966885/1‑150 (MQ=60)
                                                                                                                                   GCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTT                     <  SRR19973073.3940705/150‑1 (MQ=60)
                                                                                                                                   GCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTT                     >  SRR19973073.1009028/1‑150 (MQ=60)
                                                                                                                                      AACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTAC                  >  SRR19973073.2961603/1‑150 (MQ=60)
                                                                                                                                       ACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACG                 <  SRR19973073.1843650/150‑1 (MQ=60)
                                                                                                                                        CCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGC                >  SRR19973073.1209389/1‑150 (MQ=60)
                                                                                                                                         CAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCATTAACTGATGTCATTAATTTTACGCC               >  SRR19973073.1013281/1‑150 (MQ=60)
                                                                                                                                                CTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACT        >  SRR19973073.1493202/1‑150 (MQ=60)
                                                                                                                                                  ATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCA      >  SRR19973073.4601248/1‑150 (MQ=60)
                                                                                                                                                  ATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCA      >  SRR19973073.995830/1‑150 (MQ=60)
                                                                                                                                                    TG‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAA   <  SRR19973073.744828/150‑1 (MQ=60)
                                                                                                                                                    TG‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAA   >  SRR19973073.1673650/1‑150 (MQ=60)
                                                                                                                                                     G‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  SRR19973073.1384299/1‑150 (MQ=60)
                                                                                                                                                     G‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  SRR19973073.1384283/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  CP009273/2586880‑2587178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑