Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,587,029:1 +G 100% coding (101/699 nt) ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,587,0291.G99.1% 900.7 / 0.5 221coding (101/699 nt)ypfHpalmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (104/115);  total (104/117)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

ATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGA  >  CP009273/2586882‑2587170
                                                                                                                                                    |                                                                                                                                             
aTCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAttt                                                                                                                                                <  2:3216435/148‑1 (MQ=255)
 tCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTAtttg                                                                                                                                               >  2:793982/1‑147 (MQ=255)
   gCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gcc                                                                                                                                            <  1:2157741/148‑1 (MQ=255)
    cATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gccc                                                                                                                                           >  1:3196324/1‑148 (MQ=255)
      tCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gccccc                                                                                                                                         >  2:3597669/1‑147 (MQ=255)
       ccACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gccccca                                                                                                                                        <  1:1059446/148‑3 (MQ=255)
       ccACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑gccccca                                                                                                                                        <  1:2963023/148‑3 (MQ=255)
           acGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAACCAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGc                                                                                                                                     >  1:1994278/1‑148 (MQ=255)
           acGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGc                                                                                                                                     >  1:513587/1‑148 (MQ=255)
                      gATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTAt                                                                                                                          <  1:839952/148‑1 (MQ=255)
                       aTTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATc                                                                                                                         >  2:1823358/1‑148 (MQ=255)
                       aTTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATc                                                                                                                         >  2:651651/1‑148 (MQ=255)
                        ttATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATcc                                                                                                                        >  2:3564318/1‑148 (MQ=255)
                        ttATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATcc                                                                                                                        >  2:2550323/1‑148 (MQ=255)
                           tCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCg                                                                                                                     >  2:3439460/1‑148 (MQ=255)
                             ttCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGac                                                                                                                   >  1:3282709/1‑148 (MQ=255)
                              tCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGaca                                                                                                                  >  1:2032221/1‑148 (MQ=255)
                                 gTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCg                                                                                                               <  1:244069/148‑1 (MQ=255)
                                  tAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGt                                                                                                              >  1:2361109/1‑148 (MQ=255)
                                   aaTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGCGTTATCCCCGACACCgag                                                                                                             >  1:2838520/1‑146 (MQ=255)
                                    aTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTgg                                                                                                            >  2:210582/1‑148 (MQ=255)
                                    aTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTgg                                                                                                            >  1:3761262/1‑148 (MQ=255)
                                    aTACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTgg                                                                                                            >  1:161540/1‑148 (MQ=255)
                                     tACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGa                                                                                                           >  2:1396361/1‑148 (MQ=255)
                                     tACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGa                                                                                                           <  1:811878/148‑1 (MQ=255)
                                      aCCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaa                                                                                                          >  1:3147560/1‑148 (MQ=255)
                                      aCCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaa                                                                                                          >  2:1349608/1‑148 (MQ=255)
                                       cccTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaaa                                                                                                         <  1:1675704/148‑1 (MQ=255)
                                        ccTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGaaaa                                                                                                        <  2:61844/148‑1 (MQ=255)
                                              ccGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTa                                                                                                  <  2:1991740/148‑1 (MQ=255)
                                               cGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaa                                                                                                 <  2:3689221/148‑1 (MQ=255)
                                                  aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaa                                                                                              <  1:3190465/148‑1 (MQ=255)
                                                  aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaa                                                                                              >  1:1111229/1‑148 (MQ=255)
                                                  aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaa                                                                                              >  1:3020876/1‑148 (MQ=255)
                                                  aaaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaa                                                                                              <  1:1285689/148‑1 (MQ=255)
                                                   aaCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTaacaac                                                                                             >  1:308494/1‑148 (MQ=255)
                                                    aCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACt                                                                                            >  1:1815916/1‑148 (MQ=255)
                                                    aCCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACt                                                                                            <  2:3077194/148‑1 (MQ=255)
                                                     ccACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTg                                                                                           <  1:1220799/148‑1 (MQ=255)
                                                     ccACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTg                                                                                           >  2:3695844/1‑148 (MQ=255)
                                                       aCTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGtt                                                                                         <  2:1262201/148‑1 (MQ=255)
                                                       aCTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGtt                                                                                         >  2:3516330/1‑148 (MQ=255)
                                                           aCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcgc                                                                                     <  1:3458155/148‑1 (MQ=255)
                                                           aCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcgc                                                                                     >  1:2443412/1‑148 (MQ=255)
                                                           aCGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTgcgc                                                                                     <  2:3794217/148‑1 (MQ=255)
                                                            cGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCa                                                                                    <  1:736480/148‑1 (MQ=255)
                                                              ccccGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAgg                                                                                  <  1:2590997/148‑1 (MQ=255)
                                                               cccGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGt                                                                                 <  2:1665846/148‑1 (MQ=255)
                                                                 cgccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGttt                                                                               <  2:1896596/148‑1 (MQ=255)
                                                                   ccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                             >  1:2824369/1‑148 (MQ=255)
                                                                   ccgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAt                                                                             <  1:1565712/148‑1 (MQ=255)
                                                                    cgGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATc                                                                            <  1:3620513/148‑1 (MQ=255)
                                                                      gATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCg                                                                          >  1:683660/1‑148 (MQ=255)
                                                                      gATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCg                                                                          <  2:3282709/148‑1 (MQ=255)
                                                                       aTTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCgg                                                                         >  2:3219195/1‑148 (MQ=255)
                                                                          aCCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCt                                                                      <  1:651651/148‑1 (MQ=255)
                                                                          aCCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCt                                                                      >  2:2895102/1‑148 (MQ=255)
                                                                           ccGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCtt                                                                     <  1:3532731/148‑1 (MQ=255)
                                                                           ccGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCtt                                                                     <  2:2319926/148‑1 (MQ=255)
                                                                              cTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGa                                                                  <  2:829320/148‑1 (MQ=255)
                                                                              cTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGa                                                                  <  2:2592468/148‑1 (MQ=255)
                                                                                cGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaac                                                                >  2:2943764/1‑148 (MQ=255)
                                                                                cGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaac                                                                >  2:969203/1‑148 (MQ=255)
                                                                                   cTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGaacaac                                                             >  1:710368/1‑148 (MQ=255)
                                                                                            aCCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTc                                                    <  2:2228629/148‑1 (MQ=255)
                                                                                             ccGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCa                                                   <  2:619511/148‑1 (MQ=255)
                                                                                             ccGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCa                                                   <  2:3657146/148‑1 (MQ=255)
                                                                                               gACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATg                                                 <  1:1851570/148‑1 (MQ=255)
                                                                                               gACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATg                                                 <  1:1510115/148‑1 (MQ=255)
                                                                                                acacTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGt                                                <  2:21803/148‑1 (MQ=255)
                                                                                                acacTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGt                                                <  1:751272/148‑1 (MQ=255)
                                                                                                acacTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGt                                                <  1:1784119/148‑1 (MQ=255)
                                                                                                  acTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGttt                                              >  2:990793/1‑148 (MQ=255)
                                                                                                   cTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTc                                             <  2:1109183/148‑1 (MQ=255)
                                                                                                    tCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCa                                            <  2:1336012/148‑1 (MQ=255)
                                                                                                    tCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCa                                            <  2:934393/148‑1 (MQ=255)
                                                                                                          ccaGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGa                                      >  1:399702/1‑148 (MQ=255)
                                                                                                          ccaGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGa                                      >  1:2386754/1‑148 (MQ=255)
                                                                                                          ccaGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGa                                      >  1:402613/1‑148 (MQ=255)
                                                                                                           caGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGaa                                     <  1:1197043/148‑1 (MQ=255)
                                                                                                              cgcATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTc                                  <  2:3223341/148‑1 (MQ=255)
                                                                                                                cATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCt                                >  2:3659700/1‑148 (MQ=255)
                                                                                                                cATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCt                                >  2:2769433/1‑148 (MQ=255)
                                                                                                                  tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTa                              <  1:2728256/148‑1 (MQ=255)
                                                                                                                  tCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTa                              >  1:3672804/1‑148 (MQ=255)
                                                                                                                   cAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTaa                             <  2:1075708/148‑1 (MQ=255)
                                                                                                                    aGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAAc                            >  2:1980690/1‑148 (MQ=255)
                                                                                                                    aGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAAc                            <  2:2603863/148‑1 (MQ=255)
                                                                                                                     ggAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACt                           >  1:2437381/1‑148 (MQ=255)
                                                                                                                      gAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTg                          <  2:2473146/148‑1 (MQ=255)
                                                                                                                         aCAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATg                       <  2:2500746/148‑1 (MQ=255)
                                                                                                                           aGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTc                     <  1:2217475/148‑1 (MQ=255)
                                                                                                                            gCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCa                    <  2:438094/148‑1 (MQ=255)
                                                                                                                             cGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCAt                   <  2:3777329/148‑1 (MQ=255)
                                                                                                                               gTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTa                 <  1:2724780/148‑1 (MQ=255)
                                                                                                                                tGCAAACCAGTTGGCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAATCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTaa                <  1:2059497/148‑1 (MQ=255)
                                                                                                                                    aaCCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAtttt            <  1:1369122/148‑1 (MQ=255)
                                                                                                                                     aCCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTa           <  1:150148/148‑1 (MQ=255)
                                                                                                                                     aCCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTa           >  1:839837/1‑148 (MQ=255)
                                                                                                                                        aGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTAcgc        <  1:2280503/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGA  >  CP009273/2586882‑2587170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  CP009273/2586880‑2587178
                                                                                                                                                     |                                                                                                                                                      
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT                                                                                                                                                        <  SRR19973069.2255216/150‑1 (MQ=60)
    CGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑GCCC                                                                                                                                                   >  SRR19973069.3217015/1‑150 (MQ=60)
    CGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑GCCC                                                                                                                                                   >  SRR19973069.3217081/1‑150 (MQ=60)
     GCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑GCCCC                                                                                                                                                  <  SRR19973069.2172507/150‑1 (MQ=60)
     GCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑GCCCC                                                                                                                                                  <  SRR19973069.2172514/150‑1 (MQ=60)
       ATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCA                                                                                                                                                 <  SRR19973069.303673/150‑1 (MQ=60)
       ATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCA                                                                                                                                                 >  SRR19973069.227991/1‑150 (MQ=60)
         CCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATT                                                                                                                                               <  SRR19973069.2982683/150‑1 (MQ=60)
         CCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATT                                                                                                                                               <  SRR19973069.1066814/150‑1 (MQ=60)
           ACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAACCAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGC                                                                                                                                             >  SRR19973069.2008172/1‑150 (MQ=60)
           ACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGC                                                                                                                                             >  SRR19973069.517241/1‑150 (MQ=60)
            CACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCC                                                                                                                                            <  SRR19973069.1513601/150‑1 (MQ=60)
                  CCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGG                                                                                                                                      >  SRR19973069.1671012/1‑150 (MQ=60)
                        GATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCC                                                                                                                                <  SRR19973069.845849/150‑1 (MQ=60)
                        GATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCC                                                                                                                                <  SRR19973069.845848/150‑1 (MQ=60)
                           TATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCG                                                                                                                             >  SRR19973069.2621246/1‑150 (MQ=60)
                            ATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGA                                                                                                                            >  SRR19973069.3206303/1‑150 (MQ=60)
                             TCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGAC                                                                                                                           >  SRR19973069.3304212/1‑150 (MQ=60)
                              CTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACA                                                                                                                          >  SRR19973069.2046333/1‑150 (MQ=60)
                                  CGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGT                                                                                                                      >  SRR19973069.2377142/1‑150 (MQ=60)
                                  CGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGT                                                                                                                      >  SRR19973069.2377155/1‑150 (MQ=60)
                                  CGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGT                                                                                                                      >  SRR19973069.2377284/1‑150 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTG                                                                                                                     <  SRR19973069.245955/150‑1 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTG                                                                                                                     >  SRR19973069.3801859/1‑150 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTG                                                                                                                     <  SRR19973069.245849/150‑1 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTG                                                                                                                     <  SRR19973069.245886/150‑1 (MQ=60)
                                   GTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGCGTTATCCCCGACACCGAG                                                                                                                     >  SRR19973069.2857255/1‑150 (MQ=60)
                                    TAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGG                                                                                                                    >  SRR19973069.3785715/1‑150 (MQ=60)
                                    TAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGG                                                                                                                    >  SRR19973069.162943/1‑150 (MQ=60)
                                      ATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  >  SRR19973069.1198766/1‑150 (MQ=60)
                                      ATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  >  SRR19973069.575499/1‑150 (MQ=60)
                                      ATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  >  SRR19973069.3168143/1‑150 (MQ=60)
                                      ATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAA                                                                                                                  >  SRR19973069.3583853/1‑150 (MQ=60)
                                       TACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAA                                                                                                                 >  SRR19973069.2848776/1‑150 (MQ=60)
                                       TACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAA                                                                                                                 <  SRR19973069.817618/150‑1 (MQ=60)
                                         CCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAG                                                                                                               <  SRR19973069.1687374/150‑1 (MQ=60)
                                            TGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAG                                                                                                            >  SRR19973069.1212096/1‑150 (MQ=60)
                                            TGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAG                                                                                                            >  SRR19973069.1212127/1‑150 (MQ=60)
                                             GCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGT                                                                                                           <  SRR19973069.1554945/150‑1 (MQ=60)
                                               ACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAA                                                                                                         <  SRR19973069.1506288/150‑1 (MQ=60)
                                                 CGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACA                                                                                                       <  SRR19973069.778196/150‑1 (MQ=60)
                                                 CGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACA                                                                                                       >  SRR19973069.2965642/1‑150 (MQ=60)
                                                  GAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAA                                                                                                      >  SRR19973069.3040911/1‑150 (MQ=60)
                                                  GAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAA                                                                                                      >  SRR19973069.1118983/1‑150 (MQ=60)
                                                   AAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAAC                                                                                                     >  SRR19973069.311037/1‑150 (MQ=60)
                                                   AAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAAC                                                                                                     >  SRR19973069.310562/1‑150 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    <  SRR19973069.3211181/150‑1 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    >  SRR19973069.1083195/1‑150 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    <  SRR19973069.1294285/150‑1 (MQ=60)
                                                    AAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACT                                                                                                    >  SRR19973069.1828669/1‑150 (MQ=60)
                                                     AACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTG                                                                                                   >  SRR19973069.1599662/1‑150 (MQ=60)
                                                     AACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTG                                                                                                   <  SRR19973069.501331/150‑1 (MQ=60)
                                                       CCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTT                                                                                                 <  SRR19973069.1229150/150‑1 (MQ=60)
                                                           TGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGC                                                                                             >  SRR19973069.2459861/1‑150 (MQ=60)
                                                            GACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCA                                                                                            >  SRR19973069.3233777/1‑150 (MQ=60)
                                                            GACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCA                                                                                            >  SRR19973069.1924257/1‑150 (MQ=60)
                                                             ACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAG                                                                                           <  SRR19973069.3480549/150‑1 (MQ=60)
                                                              CGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGG                                                                                          <  SRR19973069.741615/150‑1 (MQ=60)
                                                              CGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGG                                                                                          <  SRR19973069.741697/150‑1 (MQ=60)
                                                              CGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGG                                                                                          <  SRR19973069.3017719/150‑1 (MQ=60)
                                                               GCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGT                                                                                         >  SRR19973069.2517447/1‑150 (MQ=60)
                                                                CCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTT                                                                                        <  SRR19973069.2608273/150‑1 (MQ=60)
                                                                 CCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTT                                                                                       <  SRR19973069.3501479/150‑1 (MQ=60)
                                                                  CCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTA                                                                                      >  SRR19973069.812541/1‑150 (MQ=60)
                                                                   CGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTAT                                                                                     >  SRR19973069.2843046/1‑150 (MQ=60)
                                                                     CCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCC                                                                                   <  SRR19973069.2179595/150‑1 (MQ=60)
                                                                     CCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCC                                                                                   <  SRR19973069.3452997/150‑1 (MQ=60)
                                                                     CCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCC                                                                                   <  SRR19973069.1576386/150‑1 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973069.688563/1‑150 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  <  SRR19973069.3644170/150‑1 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973069.688137/1‑150 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973069.688419/1‑150 (MQ=60)
                                                                      CGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCG                                                                                  >  SRR19973069.1790734/1‑150 (MQ=60)
                                                                        GAGTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACCGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGG                                                                                <  SRR19973069.1616119/150‑1 (MQ=60)
                                                                        GAGTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGG                                                                                <  SRR19973069.3089728/150‑1 (MQ=60)
                                                                          TTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCT                                                                              <  SRR19973069.2358601/150‑1 (MQ=60)
                                                                           TACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTT                                                                             <  SRR19973069.2082082/150‑1 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973069.655122/150‑1 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973069.1851711/150‑1 (MQ=60)
                                                                            ACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTT                                                                            <  SRR19973069.655993/150‑1 (MQ=60)
                                                                             CCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTG                                                                           <  SRR19973069.3555622/150‑1 (MQ=60)
                                                                             CCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTG                                                                           <  SRR19973069.3555630/150‑1 (MQ=60)
                                                                                   GGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAAC                                                                     >  SRR19973069.715358/1‑150 (MQ=60)
                                                                                            CCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCTCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTC                                                            >  SRR19973069.3721033/1‑150 (MQ=60)
                                                                                            CCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTC                                                            >  SRR19973069.1472617/1‑150 (MQ=60)
                                                                                                 GACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTT                                                       <  SRR19973069.1864497/150‑1 (MQ=60)
                                                                                                 GACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTT                                                       <  SRR19973069.1520555/150‑1 (MQ=60)
                                                                                                  ACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTT                                                      <  SRR19973069.756591/150‑1 (MQ=60)
                                                                                                  ACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTT                                                      <  SRR19973069.1796685/150‑1 (MQ=60)
                                                                                                  ACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTT                                                      <  SRR19973069.755969/150‑1 (MQ=60)
                                                                                                      TCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATG                                                  >  SRR19973069.1335858/1‑150 (MQ=60)
                                                                                                       CACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGG                                                 <  SRR19973069.2033692/150‑1 (MQ=60)
                                                                                                         CCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCG                                               >  SRR19973069.1865277/1‑150 (MQ=60)
                                                                                                          CACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGA                                              >  SRR19973069.402636/1‑150 (MQ=60)
                                                                                                          CACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGA                                              >  SRR19973069.402625/1‑150 (MQ=60)
                                                                                                          CACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGA                                              >  SRR19973069.405564/1‑150 (MQ=60)
                                                                                                          CACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGA                                              >  SRR19973069.2402988/1‑150 (MQ=60)
                                                                                                          CACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGA                                              >  SRR19973069.2402933/1‑150 (MQ=60)
                                                                                                             CAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACT                                           <  SRR19973069.1205278/150‑1 (MQ=60)
                                                                                                                  CATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTA                                      >  SRR19973069.3696834/1‑150 (MQ=60)
                                                                                                                  CATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTA                                      >  SRR19973069.3696835/1‑150 (MQ=60)
                                                                                                                   ATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAA                                     >  SRR19973069.3807970/1‑150 (MQ=60)
                                                                                                                    TCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAAC                                    <  SRR19973069.2746529/150‑1 (MQ=60)
                                                                                                                    TCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAAC                                    <  SRR19973069.2746528/150‑1 (MQ=60)
                                                                                                                    TCAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAAC                                    <  SRR19973069.2746512/150‑1 (MQ=60)
                                                                                                                     CAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACT                                   >  SRR19973069.2453809/1‑150 (MQ=60)
                                                                                                                     CAGGAAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACT                                   >  SRR19973069.2453901/1‑150 (MQ=60)
                                                                                                                         AAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATG                               <  SRR19973069.877647/150‑1 (MQ=60)
                                                                                                                         AAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATG                               >  SRR19973069.1801509/1‑150 (MQ=60)
                                                                                                                         AAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATG                               >  SRR19973069.1802432/1‑150 (MQ=60)
                                                                                                                         AAACAGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATG                               <  SRR19973069.2470524/150‑1 (MQ=60)
                                                                                                                             AGCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCAT                           <  SRR19973069.2232483/150‑1 (MQ=60)
                                                                                                                              GCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATT                          <  SRR19973069.2771510/150‑1 (MQ=60)
                                                                                                                              GCGGTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATT                          <  SRR19973069.845005/150‑1 (MQ=60)
                                                                                                                                 GTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAT                       <  SRR19973069.2743035/150‑1 (MQ=60)
                                                                                                                                 GTGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAAT                       <  SRR19973069.3475801/150‑1 (MQ=60)
                                                                                                                                  TGCAAACCAGTTGGCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAATCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATT                      <  SRR19973069.2073780/150‑1 (MQ=60)
                                                                                                                                  TGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATT                      <  SRR19973069.2073701/150‑1 (MQ=60)
                                                                                                                                  TGCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATT                      <  SRR19973069.888462/150‑1 (MQ=60)
                                                                                                                                   GCAAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTT                     <  SRR19973069.727614/150‑1 (MQ=60)
                                                                                                                                     AAACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTA                   >  SRR19973069.845733/1‑150 (MQ=60)
                                                                                                                                      AACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTAC                  <  SRR19973069.1378647/150‑1 (MQ=60)
                                                                                                                                       ACCAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACG                 <  SRR19973069.151475/150‑1 (MQ=60)
                                                                                                                                         CAGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCC               >  SRR19973069.3190106/1‑150 (MQ=60)
                                                                                                                                          AGTTGCCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCG              <  SRR19973069.2295989/150‑1 (MQ=60)
                                                                                                                                               CCTATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGAC         <  SRR19973069.1242603/150‑1 (MQ=60)
                                                                                                                                                 TATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTC       >  SRR19973069.690448/1‑150 (MQ=60)
                                                                                                                                                 TATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTC       >  SRR19973069.2888070/1‑150 (MQ=60)
                                                                                                                                                 TATTTGCCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTC       >  SRR19973069.2888075/1‑150 (MQ=60)
                                                                                                                                                     G‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  SRR19973069.3359176/1‑150 (MQ=60)
                                                                                                                                                     G‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  SRR19973069.3359512/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
TGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTGACGCCCCGCCGGATTACCGCTCGGCTCCGCGCCACCGACACTCACCACCAGCGCATCAGGAAACAGCGGTGCAAACCAGTTGCCTATTT‑CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAAT  >  CP009273/2586880‑2587178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑