Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,399,210 G→T 100% S304* (TCA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,399,2100GT100.0% 739.4 / NA 225S304* (TCA→TAA) lrhAtranscriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (100/125);  total (100/125)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

TACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGT  >  CP009273/2399065‑2399353
                                                                                                                                                 |                                                                                                                                               
tACGTTCTGCACTATGCTATGATGGATTTTAAATAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATGGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTaa                                                                                                                                               <  1:459226/148‑1 (MQ=255)
tACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTaa                                                                                                                                               <  1:3112227/148‑1 (MQ=255)
tACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTAAACTATTGTCTCAGGAAATATCTATAGTCCGTCGATTACTCGATATCCCTTTAAATAAACAATTaa                                                                                                                                               >  1:794525/1‑148 (MQ=255)
 aCGTTCTTCACTATGCTATTAAGTTTTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAat                                                                                                                                              <  2:2288361/148‑1 (MQ=255)
 aCGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAat                                                                                                                                              <  1:584910/148‑1 (MQ=255)
 aCGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATATATAGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAat                                                                                                                                              >  1:3657559/1‑148 (MQ=255)
    ttCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAatcat                                                                                                                                           >  2:390893/1‑148 (MQ=255)
       tgaaatATGCGATTAAGTTTTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATccc                                                                                                                                        <  1:762591/143‑1 (MQ=255)
       tGCAGTATGGTATGATGTATTTTAAATAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATccc                                                                                                                                        <  1:2937598/148‑1 (MQ=255)
        gcacactGCGATGAAGTATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCCTCCGTCGATTACTCGATATCCCTTTCAATCCACAATTAATCATcccc                                                                                                                                       <  2:679531/142‑1 (MQ=255)
        gCACTATTATATGAAGGTTTTTTACTAAAAAAAAGCCTCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATcccc                                                                                                                                       <  2:2545989/148‑1 (MQ=255)
        gCACTATGCTATTAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATcccc                                                                                                                                       <  2:1693552/148‑1 (MQ=255)
           cTATGCGATGATGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTc                                                                                                                                    <  1:2416115/148‑1 (MQ=255)
           cTATGCGATGAAGTATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTc                                                                                                                                    <  1:2415631/148‑1 (MQ=255)
              tGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCgg                                                                                                                                 <  2:3282379/148‑1 (MQ=255)
              tGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCgg                                                                                                                                 >  2:451261/1‑148 (MQ=255)
              tGCGAGGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCCTCCCCTTCCgg                                                                                                                                 >  2:1488768/1‑148 (MQ=255)
                    gAAGGATTTTAACTAAAAAAACGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCTGAGCAGa                                                                                                                           <  1:1414375/148‑1 (MQ=255)
                       ggATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACAt                                                                                                                        <  1:3146055/148‑1 (MQ=255)
                        gATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACAtt                                                                                                                       <  2:2495781/148‑1 (MQ=255)
                               aatAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGt                                                                                                                <  1:1968165/146‑1 (MQ=255)
                                 tAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTAt                                                                                                              <  2:1935151/148‑1 (MQ=255)
                                 tAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTAt                                                                                                              >  2:3436861/1‑148 (MQ=255)
                                      aaaaGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCa                                                                                                         <  1:3738125/148‑1 (MQ=255)
                                         aGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCCGACCGTGGGCTGTATTGCCCCgg                                                                                                      >  2:3002294/1‑148 (MQ=255)
                                         aGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATAATCCCCTTCCGGAGCAGACATTTGGCTGTATTTCCACgg                                                                                                      >  2:717400/1‑148 (MQ=255)
                                          gCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGa                                                                                                     <  2:2821698/148‑1 (MQ=255)
                                          gCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGa                                                                                                     <  1:3389973/148‑1 (MQ=255)
                                             gCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTa                                                                                                  <  2:1166146/148‑1 (MQ=255)
                                             gCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTa                                                                                                  <  1:1450725/148‑1 (MQ=255)
                                              cTGGGGTTTAACACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTAt                                                                                                 <  1:1492674/148‑1 (MQ=255)
                                              cTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTAt                                                                                                 <  1:2459963/148‑1 (MQ=255)
                                              cTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTAt                                                                                                 <  2:2759073/148‑1 (MQ=255)
                                                 gggTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGt                                                                                              <  1:2486310/148‑1 (MQ=255)
                                                 gggTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGt                                                                                              >  1:1889588/1‑148 (MQ=255)
                                                   gTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAg                                                                                            <  1:89089/148‑1 (MQ=255)
                                                   gTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAg                                                                                            <  1:707821/148‑1 (MQ=255)
                                                    tttAAAACACCCCCAGCGGCTCGTTTTTTTAACTATTGTGTCAGGAATTATCTATCGTGCGTCGCTTAGTCGATATCCCTTTGAATAAACAATTAATCATACCCGTCCGGAGCAGACCATGGGCTGTATTTCCCCGGGTTATGGTAGc                                                                                           >  1:1025787/1‑148 (MQ=255)
                                                        aaaCACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCtctc                                                                                       <  2:2807482/148‑1 (MQ=255)
                                                          acacCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCATCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCa                                                                                     >  1:3796650/1‑148 (MQ=255)
                                                           cacCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCAt                                                                                    >  2:2035566/1‑148 (MQ=255)
                                                                 cAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTg                                                                              <  2:3513472/148‑1 (MQ=255)
                                                                    cGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATa                                                                           >  2:1892285/1‑148 (MQ=255)
                                                                    cGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATa                                                                           >  2:1892379/1‑148 (MQ=255)
                                                                    cGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATa                                                                           >  1:2228941/1‑148 (MQ=255)
                                                                      gCTCGTTTTTTACACTAGTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGa                                                                         <  1:3762651/148‑1 (MQ=255)
                                                                       cTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAt                                                                        <  1:3339571/148‑1 (MQ=255)
                                                                       cTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAt                                                                        >  1:3326512/1‑148 (MQ=255)
                                                                        tCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATc                                                                       >  2:1623796/1‑148 (MQ=255)
                                                                        tCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATc                                                                       >  1:117371/1‑148 (MQ=255)
                                                                            tttttACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCt                                                                   >  2:1551372/1‑148 (MQ=255)
                                                                                tACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGc                                                               <  2:3646294/148‑1 (MQ=255)
                                                                                   acTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAg                                                            >  1:720553/1‑148 (MQ=255)
                                                                                    cTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGc                                                           >  2:2853821/1‑148 (MQ=255)
                                                                                     tATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCt                                                          <  1:3526/148‑1 (MQ=255)
                                                                                      aTTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTc                                                         >  1:763591/1‑148 (MQ=255)
                                                                                       ttGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCa                                                        <  2:2007082/148‑1 (MQ=255)
                                                                                        tGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCat                                                       <  1:1188613/148‑1 (MQ=255)
                                                                                        tGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCat                                                       >  2:1086518/1‑148 (MQ=255)
                                                                                        tGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCat                                                       >  2:2405286/1‑148 (MQ=255)
                                                                                        tGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCat                                                       <  2:3795046/148‑1 (MQ=255)
                                                                                         gTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCatt                                                      <  1:2743636/148‑1 (MQ=255)
                                                                                               ggAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACt                                                <  2:156785/148‑1 (MQ=255)
                                                                                                  aTTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGa                                             <  2:3376686/148‑1 (MQ=255)
                                                                                                  aTTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGa                                             >  2:121338/1‑148 (MQ=255)
                                                                                                   ttATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAc                                            >  2:3598703/1‑148 (MQ=255)
                                                                                                     atctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACgg                                          <  2:3047778/148‑1 (MQ=255)
                                                                                                     atctatcGTCCGTCGATTACTAGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACgg                                          <  2:3047812/148‑1 (MQ=255)
                                                                                                      tctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGa                                         >  1:2368147/1‑148 (MQ=255)
                                                                                                       ctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAt                                        <  2:49775/148‑1 (MQ=255)
                                                                                                        tatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATc                                       >  1:2817839/1‑148 (MQ=255)
                                                                                                          tcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCAt                                     >  1:3745081/1‑148 (MQ=255)
                                                                                                           cgtccgtcGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATa                                    >  1:2557431/1‑148 (MQ=255)
                                                                                                             tccgtcGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGc                                  >  2:1144189/1‑148 (MQ=255)
                                                                                                                  cGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGc                             >  1:921103/1‑148 (MQ=255)
                                                                                                                    aTTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaa                           <  1:2102808/148‑1 (MQ=255)
                                                                                                                    aTTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaa                           >  1:2036203/1‑148 (MQ=255)
                                                                                                                     ttACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaaa                          >  1:3211012/1‑148 (MQ=255)
                                                                                                                     ttACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaaa                          >  2:1711333/1‑148 (MQ=255)
                                                                                                                     ttACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaaa                          <  1:3436861/148‑1 (MQ=255)
                                                                                                                     ttACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCaaa                          >  1:3691301/1‑148 (MQ=255)
                                                                                                                        cTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAatat                       <  2:1399432/148‑1 (MQ=255)
                                                                                                                         tCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATAtt                      >  2:1868418/1‑148 (MQ=255)
                                                                                                                          cGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTc                     >  1:1398274/1‑148 (MQ=255)
                                                                                                                          cGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTc                     <  1:2013520/148‑1 (MQ=255)
                                                                                                                            atatCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCgg                   >  2:818735/1‑148 (MQ=255)
                                                                                                                            atatCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCgg                   >  1:768038/1‑148 (MQ=255)
                                                                                                                             tatCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGt                  <  2:3657559/148‑1 (MQ=255)
                                                                                                                              atCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTa                 >  1:1563988/1‑148 (MQ=255)
                                                                                                                                cccTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATc               >  2:1279901/1‑148 (MQ=255)
                                                                                                                                cccTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATc               >  2:575693/1‑148 (MQ=255)
                                                                                                                                 ccTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCa              <  1:2110639/148‑1 (MQ=255)
                                                                                                                                  cTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAg             <  2:3714371/148‑1 (MQ=255)
                                                                                                                                  cTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAg             <  2:2355616/148‑1 (MQ=255)
                                                                                                                                        atcaaCAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAgcg       >  2:3403050/1‑148 (MQ=255)
                                                                                                                                         tcaaCAATTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAgcgg      >  1:400664/1‑148 (MQ=255)
                                                                                                                                          caacaaTTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAgcggc     >  1:1747703/1‑148 (MQ=255)
                                                                                                                                          caacaaTTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAgcggc     >  2:2180407/1‑148 (MQ=255)
                                                                                                                                           aacaaTTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAgcggcg    <  1:2723066/148‑1 (MQ=255)
                                                                                                                                             caaTTAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGt  >  1:3257082/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGT  >  CP009273/2399065‑2399353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAG  >  CP009273/2399063‑2399356
                                                                                                                                                   |                                                                                                                                                   
TGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTAAACTATTGTCTCAGGAAATATCTATAGTCCGTCGATTACTCGATATCCCTTTAAATAAACAATTAA                                                                                                                                                   >  SRR19973069.800158/1‑150 (MQ=60)
 TTACGTTCTGAACTATGCGATCATGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAAT                                                                                                                                                  <  SRR19973069.870451/150‑1 (MQ=60)
 GTACTTTCGGCTCTATGCGATTAATTATTTTTACAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAAT                                                                                                                                                  <  SRR19973069.3635166/150‑1 (MQ=60)
 GTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATATATAGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAAT                                                                                                                                                  >  SRR19973069.3681509/1‑150 (MQ=60)
  TACGTTCTGCACTATGCTATGATGGATTTTAAATAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATGGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATC                                                                                                                                                 <  SRR19973069.462588/150‑1 (MQ=60)
  TACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATC                                                                                                                                                 <  SRR19973069.3132693/150‑1 (MQ=60)
   ACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCA                                                                                                                                                <  SRR19973069.588912/150‑1 (MQ=60)
     GTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATC                                                                                                                                              >  SRR19973069.3502560/1‑150 (MQ=60)
     GTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACAATATTGTCTAAGGAATTATATATAGTCCGTCGATTACTCGATATCCCTTTAAATCAACAATTAATCATA                                                                                                                                              >  SRR19973069.2021053/1‑150 (MQ=60)
        CTGCACTAGGCTATGAAGTTTTTTTAAAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCC                                                                                                                                           <  SRR19973069.77968/150‑1 (MQ=60)
         ttcactttgcttttattgtTTTTTAATAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCT                                                                                                                                          <  SRR19973069.1035453/131‑1 (MQ=60)
         tgcagtatggtatgatgtattttaaaTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCT                                                                                                                                          <  SRR19973069.2957079/124‑1 (MQ=60)
         tgaaatatgcgattaagttTTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCT                                                                                                                                          <  SRR19973069.767976/131‑1 (MQ=60)
         TGCACTCTGCGATTATGTATTTTTAAAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCT                                                                                                                                          <  SRR19973069.767999/150‑1 (MQ=60)
         TGCACTCTGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCT                                                                                                                                          <  SRR19973069.2189806/150‑1 (MQ=60)
          gaactctgctattatgtatttttaaaAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTT                                                                                                                                         <  SRR19973069.3012279/124‑1 (MQ=60)
            ACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTTCACGATTTTCTCAGGAATTTTATATAGTGCGGCGATTACTCGATATCCCTTTAAATCAACAATTAATCATACCCTTCC                                                                                                                                       >  SRR19973069.1702531/1‑150 (MQ=60)
             ctttggtttttagttTTTTTAATAAAAAAACGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCG                                                                                                                                      <  SRR19973069.1449925/135‑1 (MQ=60)
             ctatcctatgaagttTTTTTACAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCG                                                                                                                                      <  SRR19973069.2431998/135‑1 (MQ=60)
             ctaggctattaagttTTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCG                                                                                                                                      <  SRR19973069.3363657/135‑1 (MQ=60)
             CTATGCGATGATGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCG                                                                                                                                      <  SRR19973069.2432439/150‑1 (MQ=60)
             CTATGCGATGAAGTATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCG                                                                                                                                      <  SRR19973069.2431953/150‑1 (MQ=60)
             CTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCAGCCCCGTCCG                                                                                                                                      >  SRR19973069.2418062/1‑150 (MQ=60)
               ATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTAAACTATTTTGTCAGGAAATATCTATAGTGCGTCGATTACTCGATATACCATTAAATCAACAATTAATAATACCCATCCGGA                                                                                                                                    >  SRR19973069.1409198/1‑150 (MQ=60)
                      gactttttttaaaaAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACA                                                                                                                            <  SRR19973069.1475780/136‑1 (MQ=60)
                      GAAGGATTTTAACTAAAAAAACGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCTGAGCAG‑ACA                                                                                                                            <  SRR19973069.1424234/150‑1 (MQ=60)
                         GGTTTTTGACTAAAAAAACGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTG                                                                                                                         <  SRR19973069.3165950/150‑1 (MQ=60)
                         GGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTG                                                                                                                         <  SRR19973069.3166632/150‑1 (MQ=60)
                           ATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGG                                                                                                                       <  SRR19973069.3371019/150‑1 (MQ=60)
                                 ACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTAT                                                                                                                 <  SRR19973069.2544567/150‑1 (MQ=60)
                                 AATAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTAT                                                                                                                 <  SRR19973069.1981828/150‑1 (MQ=60)
                                  CAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATT                                                                                                                <  SRR19973069.1518251/150‑1 (MQ=60)
                                   AAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTG                                                                                                               <  SRR19973069.773064/150‑1 (MQ=60)
                                    AAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACGATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATAATCCCCTTCCGGAGCCG‑ACATTGGGCTGTATTGC                                                                                                              >  SRR19973069.637374/1‑150 (MQ=60)
                                      AAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCA                                                                                                            <  SRR19973069.3210796/150‑1 (MQ=60)
                                       AAAACGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCAC                                                                                                           <  SRR19973069.657330/150‑1 (MQ=60)
                                        AAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACG                                                                                                          <  SRR19973069.3762489/150‑1 (MQ=60)
                                        AAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACG                                                                                                          <  SRR19973069.3760152/150‑1 (MQ=60)
                                            GCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATT                                                                                                      <  SRR19973069.3412430/150‑1 (MQ=60)
                                            GCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATT                                                                                                      <  SRR19973069.3412114/150‑1 (MQ=60)
                                               GCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATG                                                                                                   <  SRR19973069.1460854/150‑1 (MQ=60)
                                               GCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATG                                                                                                   <  SRR19973069.1461107/150‑1 (MQ=60)
                                                CTGGGGTTTAACACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGG                                                                                                  <  SRR19973069.1503032/150‑1 (MQ=60)
                                                CTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGG                                                                                                  <  SRR19973069.2476490/150‑1 (MQ=60)
                                                 TGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATACCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGT                                                                                                 >  SRR19973069.1902699/1‑150 (MQ=60)
                                                  GGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTA                                                                                                <  SRR19973069.3807009/150‑1 (MQ=60)
                                                  GGGGTTTAAAACACCCCCAGCGGCTCGATTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTA                                                                                                <  SRR19973069.3805876/150‑1 (MQ=60)
                                                   GGGTTTAACACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAG                                                                                               <  SRR19973069.80651/150‑1 (MQ=60)
                                                   GGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTTTGTCAGGAATTATCTATAGTCCGTCGCTTAATCGATATCCCATTCAATCAACCAATAATCATCCCCATCCCGAGCCG‑ACATTGGGGTGTATTTGCACGGAGTATTGTAG                                                                                               >  SRR19973069.2308074/1‑150 (MQ=60)
                                                   GGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAG                                                                                               <  SRR19973069.2502947/150‑1 (MQ=60)
                                                    GGTTTAAAACACCCCCAGCGGCTCGTTTTTTTAACTATTGTGTCAGGAATTATCTATCGTGCGTCGCTTAGTCGATATCCCTTTGAATAAACAATTAATCATACCCGTCCGGAGCAG‑ACCATGGGCTGTATTTCCCCGGGTTATGGTAGC                                                                                              >  SRR19973069.1032887/1‑150 (MQ=60)
                                                     GTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCT                                                                                             <  SRR19973069.91398/150‑1 (MQ=60)
                                                     GTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCT                                                                                             <  SRR19973069.90017/150‑1 (MQ=60)
                                                     GTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCT                                                                                             <  SRR19973069.712790/150‑1 (MQ=60)
                                                     GTTTAAAACACCCCCAGCGGCTCGTTTTTTACACGATTTTATCAGGAAATATATATAGTCCGTCGATTACTCGATATCACATTAAATAAACAATTAATAATCCCCTTCCGGTGCAGCCCATTGGG‑TGTATTTCGACGGATTATGGTAGCT                                                                                             >  SRR19973069.1001571/1‑150 (MQ=45)
                                                          AAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCATCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCA                                                                                        >  SRR19973069.3821251/1‑150 (MQ=60)
                                                                 CCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTG                                                                                 <  SRR19973069.551976/150‑1 (MQ=60)
                                                                    AGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATA                                                                              >  SRR19973069.302634/1‑150 (MQ=60)
                                                                    AGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATA                                                                              >  SRR19973069.2244001/1‑150 (MQ=60)
                                                                     GCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAG                                                                             <  SRR19973069.2759157/150‑1 (MQ=60)
                                                                      CGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGA                                                                            >  SRR19973069.1121465/1‑150 (MQ=60)
                                                                       GGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAT                                                                           >  SRR19973069.3348312/1‑150 (MQ=60)
                                                                        GCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                                                          >  SRR19973069.118504/1‑150 (MQ=60)
                                                                        GCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                                                          >  SRR19973069.118551/1‑150 (MQ=60)
                                                                        GCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                                                          >  SRR19973069.1957732/1‑150 (MQ=60)
                                                                        GCTCGTTTTTTACACTAGTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                                                          <  SRR19973069.3787112/150‑1 (MQ=60)
                                                                         CTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCA                                                                         <  SRR19973069.3361437/150‑1 (MQ=60)
                                                                          TCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCAC                                                                        <  SRR19973069.2033636/150‑1 (MQ=60)
                                                                          TCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCAC                                                                        <  SRR19973069.2033564/150‑1 (MQ=60)
                                                                                   ACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAG                                                               >  SRR19973069.3273230/1‑150 (MQ=60)
                                                                                   ACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAG                                                               >  SRR19973069.725738/1‑150 (MQ=60)
                                                                                   ACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAG                                                               >  SRR19973069.725633/1‑150 (MQ=60)
                                                                                     ACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCT                                                             <  SRR19973069.336240/150‑1 (MQ=60)
                                                                                     ACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCT                                                             <  SRR19973069.174064/150‑1 (MQ=60)
                                                                                      CTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTC                                                            >  SRR19973069.768981/1‑150 (MQ=60)
                                                                                       TATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCA                                                           >  SRR19973069.2463382/1‑150 (MQ=60)
                                                                                       TATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCA                                                           <  SRR19973069.3580/150‑1 (MQ=60)
                                                                                         TTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATT                                                         <  SRR19973069.722457/150‑1 (MQ=60)
                                                                                          TGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTA                                                        <  SRR19973069.1196816/150‑1 (MQ=60)
                                                                                           GTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTAT                                                       <  SRR19973069.2761969/150‑1 (MQ=60)
                                                                                            TCTCAGGAATTATCTATCGTGCGTCGATTACTCGATATCCCTTTCAATCTACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTTTTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGGCAGCTCATTATT                                                      <  SRR19973069.2120714/150‑1 (MQ=60)
                                                                                            TCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATT                                                      <  SRR19973069.3775341/150‑1 (MQ=60)
                                                                                               CAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACT                                                   <  SRR19973069.3686340/150‑1 (MQ=60)
                                                                                                 GGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTATCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGG                                                 <  SRR19973069.2090837/150‑1 (MQ=60)
                                                                                                   AATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAC                                               >  SRR19973069.2483827/1‑150 (MQ=60)
                                                                                                   AATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAC                                               >  SRR19973069.2483774/1‑150 (MQ=60)
                                                                                                    ATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACG                                              >  SRR19973069.413301/1‑150 (MQ=60)
                                                                                                      TATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGA                                            >  SRR19973069.2384221/1‑150 (MQ=60)
                                                                                                      TATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGA                                            >  SRR19973069.1360502/1‑150 (MQ=60)
                                                                                                       ATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAT                                           >  SRR19973069.3180490/1‑150 (MQ=60)
                                                                                                        TCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATC                                          <  SRR19973069.393767/150‑1 (MQ=60)
                                                                                                        TCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATC                                          >  SRR19973069.2836492/1‑150 (MQ=60)
                                                                                                        TCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATC                                          >  SRR19973069.2836657/1‑150 (MQ=60)
                                                                                                         CTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCA                                         >  SRR19973069.3767762/1‑150 (MQ=60)
                                                                                                          TATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCAT                                        >  SRR19973069.3769476/1‑150 (MQ=60)
                                                                                                           ATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA                                       >  SRR19973069.2574318/1‑150 (MQ=60)
                                                                                                           ATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA                                       <  SRR19973069.225153/150‑1 (MQ=60)
                                                                                                            TCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAG                                      <  SRR19973069.1438761/150‑1 (MQ=60)
                                                                                                                  GTCGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGC                                >  SRR19973069.927376/1‑150 (MQ=60)
                                                                                                                   TCGATTACTCGATATCCCTTTCAATCAACAATTAATCAGCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCA                               <  SRR19973069.270030/150‑1 (MQ=60)
                                                                                                                    CGATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAA                              >  SRR19973069.2050345/1‑150 (MQ=60)
                                                                                                                     GATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAA                             >  SRR19973069.3715441/1‑150 (MQ=60)
                                                                                                                     GATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAA                             >  SRR19973069.3231948/1‑150 (MQ=60)
                                                                                                                      ATTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAAT                            <  SRR19973069.2117325/150‑1 (MQ=60)
                                                                                                                       TTACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATA                           <  SRR19973069.3459185/150‑1 (MQ=60)
                                                                                                                        TACTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATAT                          >  SRR19973069.3395903/1‑150 (MQ=60)
                                                                                                                          CTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTC                        >  SRR19973069.3322215/1‑150 (MQ=60)
                                                                                                                          CTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTC                        >  SRR19973069.1408063/1‑150 (MQ=60)
                                                                                                                          CTCGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTC                        >  SRR19973069.1408034/1‑150 (MQ=60)
                                                                                                                            CGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGG                      <  SRR19973069.183605/150‑1 (MQ=60)
                                                                                                                            CGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGG                      <  SRR19973069.2027521/150‑1 (MQ=60)
                                                                                                                            CGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGG                      >  SRR19973069.773125/1‑150 (MQ=60)
                                                                                                                            CGATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGG                      >  SRR19973069.773462/1‑150 (MQ=60)
                                                                                                                             GATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGT                     >  SRR19973069.2266543/1‑150 (MQ=60)
                                                                                                                              ATATCCCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTA                    >  SRR19973069.1574655/1‑150 (MQ=60)
                                                                                                                                   CCTTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGG               <  SRR19973069.2125195/150‑1 (MQ=60)
                                                                                                                                     TTTCAATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTA             >  SRR19973069.3651314/1‑150 (MQ=60)
                                                                                                                                         AATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGG         >  SRR19973069.3708512/1‑150 (MQ=60)
                                                                                                                                         AATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGG         >  SRR19973069.403595/1‑150 (MQ=60)
                                                                                                                                          ATCAACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGC        >  SRR19973069.1759998/1‑150 (MQ=60)
                                                                                                                                             AACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGT     >  SRR19973069.3278415/1‑150 (MQ=60)
                                                                                                                                             AACAATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGT     <  SRR19973069.2741307/150‑1 (MQ=60)
                                                                                                                                                AATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAG  >  SRR19973069.737892/1‑150 (MQ=60)
                                                                                                                                                AATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAG  >  SRR19973069.2373666/1‑150 (MQ=60)
                                                                                                                                                AATTAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCACAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAG  >  SRR19973069.737881/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
TGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAG‑ACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAG  >  CP009273/2399063‑2399356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑