Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 433,551 G→T 100% S14Y (TCC→TAC)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273433,5510GT99.6% 900.5 / ‑4.1 252S14Y (TCC→TAC) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (125/124);  total (126/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

CAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTT  >  CP009273/433405‑433697
                                                                                                                                                  |                                                                                                                                                  
 aGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAAc                                                                                                                                                <  2:2574961/150‑1 (MQ=255)
 aGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAAc                                                                                                                                                <  2:216658/150‑1 (MQ=255)
 aGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAAc                                                                                                                                                <  2:3606698/150‑1 (MQ=255)
   aaTAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAAcgc                                                                                                                                              >  1:2373274/1‑150 (MQ=255)
    aTAACTGTTGGCGGTATCAAATAAATTTATGCCGCCTTCCAGTGCACGTTTAATTCTGGGACGGCTGATTTCTTCCGGCTGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAAcgcg                                                                                                                                             <  2:663316/150‑1 (MQ=255)
     tAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGa                                                                                                                                            >  2:3778103/1‑150 (MQ=255)
           ttGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCg                                                                                                                                      <  1:2075885/150‑1 (MQ=255)
            tGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCgg                                                                                                                                     >  2:643503/1‑150 (MQ=255)
              gCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGtt                                                                                                                                   <  2:1821983/150‑1 (MQ=255)
               cGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTTATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGttt                                                                                                                                  <  1:927677/150‑1 (MQ=255)
                ggTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGtttt                                                                                                                                 >  1:1226915/1‑150 (MQ=255)
                  tATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTc                                                                                                                               >  1:691238/1‑150 (MQ=255)
                   aTCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTcc                                                                                                                              <  2:2670277/150‑1 (MQ=255)
                    tCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCt                                                                                                                             <  2:2180400/150‑1 (MQ=255)
                     cAAAGAAATTTATGCCGCCTTCCCGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTa                                                                                                                            >  2:2804334/1‑150 (MQ=255)
                       aaGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAg                                                                                                                          <  2:4066330/150‑1 (MQ=255)
                       aaGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAg                                                                                                                          >  1:3460588/1‑150 (MQ=255)
                          aaaTTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAgggg                                                                                                                       <  2:2217364/150‑1 (MQ=255)
                          aaaTTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAgggg                                                                                                                       >  1:3445682/1‑150 (MQ=255)
                          aaaTTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAgggg                                                                                                                       >  2:3167509/1‑150 (MQ=255)
                              ttATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGt                                                                                                                   <  1:2042546/150‑1 (MQ=255)
                               tATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTa                                                                                                                  <  1:1653730/150‑1 (MQ=255)
                                 tGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTAtt                                                                                                                <  1:850/150‑1 (MQ=255)
                                  gccgccTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTg                                                                                                               >  1:3147201/1‑150 (MQ=255)
                                        ttCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCAtgct                                                                                                         <  2:1146443/150‑1 (MQ=255)
                                           cAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGcc                                                                                                      <  2:1824747/150‑1 (MQ=255)
                                                cACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTcc                                                                                                 >  2:1346301/1‑150 (MQ=255)
                                                 aCGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCt                                                                                                <  2:2038907/150‑1 (MQ=255)
                                                 aCGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCt                                                                                                >  2:3579977/1‑150 (MQ=255)
                                                  cGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTg                                                                                               <  2:3470638/150‑1 (MQ=255)
                                                  cGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTg                                                                                               <  2:981495/150‑1 (MQ=255)
                                                   gTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGc                                                                                              <  2:1678947/150‑1 (MQ=255)
                                                    tttAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCt                                                                                             <  1:722770/150‑1 (MQ=255)
                                                    tttAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCt                                                                                             >  1:2964109/1‑150 (MQ=255)
                                                    tttAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCt                                                                                             <  2:2200629/150‑1 (MQ=255)
                                                      tAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCtat                                                                                           >  2:845546/1‑150 (MQ=255)
                                                      tAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTGTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCtat                                                                                           >  2:3141111/1‑150 (MQ=255)
                                                        aTTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAc                                                                                         <  1:2226065/150‑1 (MQ=255)
                                                        aTTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAc                                                                                         >  1:196003/1‑150 (MQ=255)
                                                        aTTATGGGACGGCTGCTATCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAc                                                                                         >  1:458481/1‑150 (MQ=255)
                                                         ttATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACt                                                                                        <  2:4094062/150‑1 (MQ=255)
                                                         ttATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACt                                                                                        >  2:3660009/1‑150 (MQ=255)
                                                           aTGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCg                                                                                      >  2:2926399/1‑150 (MQ=255)
                                                            tGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGt                                                                                     <  2:1942686/150‑1 (MQ=255)
                                                                  ggCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACt                                                                               <  2:1049312/150‑1 (MQ=255)
                                                                   gctgctTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACtt                                                                              >  1:2781999/1‑150 (MQ=255)
                                                                     tgctTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCa                                                                            >  2:3928901/1‑150 (MQ=255)
                                                                      gctTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCaa                                                                           >  2:248445/1‑150 (MQ=255)
                                                                        tttCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGt                                                                         >  1:1818769/1‑150 (MQ=255)
                                                                        tttCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGt                                                                         >  1:2325076/1‑150 (MQ=255)
                                                                           cttcCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGc                                                                      >  1:3660469/1‑150 (MQ=255)
                                                                              ccGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAtg                                                                   <  1:3797920/150‑1 (MQ=255)
                                                                               cGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAtgt                                                                  >  1:2137514/1‑150 (MQ=255)
                                                                               cGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAtgt                                                                  >  1:2600428/1‑150 (MQ=255)
                                                                                ggCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAtgtg                                                                 >  1:3525185/1‑150 (MQ=255)
                                                                                   aGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGtgctg                                                              >  2:870128/1‑150 (MQ=255)
                                                                                    gtgtCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGtgctgc                                                             >  2:1188296/1‑150 (MQ=255)
                                                                                        ccATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCt                                                         <  1:1020233/150‑1 (MQ=255)
                                                                                        ccATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCt                                                         <  2:458232/150‑1 (MQ=255)
                                                                                        ccATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCt                                                         <  2:2160571/150‑1 (MQ=255)
                                                                                         cATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTg                                                        <  2:1737165/150‑1 (MQ=255)
                                                                                              gTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTc                                                   <  1:97991/150‑1 (MQ=255)
                                                                                               tGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCg                                                  >  2:2946623/1‑150 (MQ=255)
                                                                                                gATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGt                                                 >  2:2675291/1‑150 (MQ=255)
                                                                                                  ttACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTc                                               >  1:1235600/1‑150 (MQ=255)
                                                                                                  ttACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTc                                               >  1:2900287/1‑150 (MQ=255)
                                                                                                     ccGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAATGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCAcc                                            >  2:244870/1‑150 (MQ=255)
                                                                                                         gATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAg                                        >  1:1318058/1‑150 (MQ=255)
                                                                                                          aTCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGt                                       >  2:2732752/1‑150 (MQ=255)
                                                                                                          aTCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGt                                       >  1:2263062/1‑150 (MQ=255)
                                                                                                          aTCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGt                                       <  1:146448/150‑1 (MQ=255)
                                                                                                           tCTGGCTCGCCAAAGGTCATGCAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTc                                      >  1:3323328/1‑150 (MQ=255)
                                                                                                            cTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCa                                     >  1:3969956/1‑150 (MQ=255)
                                                                                                            cTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCa                                     <  1:488031/150‑1 (MQ=255)
                                                                                                               gCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCactt                                  <  2:3270769/150‑1 (MQ=255)
                                                                                                                   gCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCacttactt                              <  1:407330/150‑1 (MQ=255)
                                                                                                                   gCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCacttactt                              <  2:2259389/150‑1 (MQ=255)
                                                                                                                     cAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTAt                            >  1:3041795/1‑150 (MQ=255)
                                                                                                                      aaaGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATg                           >  2:52960/1‑150 (MQ=255)
                                                                                                                       aaGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGt                          >  1:1098867/1‑150 (MQ=255)
                                                                                                                        aGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTa                         <  2:3181424/150‑1 (MQ=255)
                                                                                                                        aGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTa                         >  2:1080754/1‑150 (MQ=255)
                                                                                                                        aGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTa                         >  1:600212/1‑150 (MQ=255)
                                                                                                                         ggTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTaa                        >  1:2844795/1‑150 (MQ=255)
                                                                                                                             aTACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTc                    <  2:422467/150‑1 (MQ=255)
                                                                                                                             aTACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTAATTATGTAAGCTc                    >  1:2663996/1‑150 (MQ=255)
                                                                                                                              tACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTcc                   <  1:3544375/150‑1 (MQ=255)
                                                                                                                                    cGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGc             >  2:2958840/1‑150 (MQ=255)
                                                                                                                                     gAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCt            <  1:2714425/150‑1 (MQ=255)
                                                                                                                                     gAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCt            >  2:546043/1‑150 (MQ=255)
                                                                                                                                      aGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCtt           <  2:1447284/150‑1 (MQ=255)
                                                                                                                                      aGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCtt           <  2:3938818/150‑1 (MQ=255)
                                                                                                                                       ggCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTc          <  2:1431816/150‑1 (MQ=255)
                                                                                                                                       ggCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTc          <  2:325911/150‑1 (MQ=255)
                                                                                                                                        gCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCa         >  2:1510732/1‑150 (MQ=255)
                                                                                                                                          aaaGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACt       >  2:127023/1‑150 (MQ=255)
                                                                                                                                          aaaGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACt       >  2:2592068/1‑150 (MQ=255)
                                                                                                                                            aGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGTTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCg     >  1:2561591/1‑150 (MQ=255)
                                                                                                                                             gTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGt    <  2:2379126/150‑1 (MQ=255)
                                                                                                                                               cGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGttt  <  2:2270868/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTT  >  CP009273/433405‑433697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGC  >  CP009273/433402‑433699
                                                                                                                                                     |                                                                                                                                                    
CGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGT                                                                                                                                                      <  SRR19973074.1026686/150‑1 (MQ=60)
 GTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTA                                                                                                                                                     >  SRR19973074.3043253/1‑150 (MQ=60)
   CAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAA                                                                                                                                                   >  SRR19973074.1875265/1‑150 (MQ=60)
      AATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGC                                                                                                                                                >  SRR19973074.2377687/1‑150 (MQ=60)
      AATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGC                                                                                                                                                >  SRR19973074.2377675/1‑150 (MQ=60)
             GTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTC                                                                                                                                         >  SRR19973074.923986/1‑150 (MQ=60)
             GTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTC                                                                                                                                         >  SRR19973074.3261246/1‑150 (MQ=60)
              TTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCG                                                                                                                                        <  SRR19973074.999146/150‑1 (MQ=60)
              TTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCG                                                                                                                                        <  SRR19973074.2079771/150‑1 (MQ=60)
              TTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCG                                                                                                                                        <  SRR19973074.2079746/150‑1 (MQ=60)
               TGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGG                                                                                                                                       <  SRR19973074.1348461/150‑1 (MQ=60)
                  CGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTTATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTT                                                                                                                                    <  SRR19973074.929441/150‑1 (MQ=60)
                   GGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTT                                                                                                                                   >  SRR19973074.342261/1‑150 (MQ=60)
                   GGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTT                                                                                                                                   >  SRR19973074.1229261/1‑150 (MQ=60)
                   GGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTT                                                                                                                                   >  SRR19973074.1229264/1‑150 (MQ=60)
                     TATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTC                                                                                                                                 >  SRR19973074.692509/1‑150 (MQ=60)
                     TATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTC                                                                                                                                 >  SRR19973074.692512/1‑150 (MQ=60)
                       TCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCT                                                                                                                               <  SRR19973074.2665777/150‑1 (MQ=60)
                          AAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAG                                                                                                                            >  SRR19973074.294102/1‑150 (MQ=60)
                          AAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAG                                                                                                                            >  SRR19973074.3467027/1‑150 (MQ=60)
                          AAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAG                                                                                                                            >  SRR19973074.3466946/1‑150 (MQ=60)
                             AAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGG                                                                                                                         >  SRR19973074.3452021/1‑150 (MQ=60)
                              AATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTAACGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGT                                                                                                                        >  SRR19973074.638095/1‑150 (MQ=60)
                                 TTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGT                                                                                                                     >  SRR19973074.3545594/1‑150 (MQ=60)
                                 TTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGT                                                                                                                     <  SRR19973074.2046478/150‑1 (MQ=60)
                                 TTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGT                                                                                                                     <  SRR19973074.2046394/150‑1 (MQ=60)
                                 TTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGT                                                                                                                     <  SRR19973074.2844273/150‑1 (MQ=60)
                                  TATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTA                                                                                                                    <  SRR19973074.1656854/150‑1 (MQ=60)
                                   ATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTAT                                                                                                                   >  SRR19973074.3664845/1‑150 (MQ=60)
                                   ATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTAT                                                                                                                   >  SRR19973074.2662599/1‑150 (MQ=60)
                                    TGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATT                                                                                                                  <  SRR19973074.851/150‑1 (MQ=60)
                                     GCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTG                                                                                                                 >  SRR19973074.3152976/1‑150 (MQ=60)
                                        GCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCAT                                                                                                              >  SRR19973074.1283875/1‑150 (MQ=60)
                                         CCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTATCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATG                                                                                                             >  SRR19973074.126265/1‑150 (MQ=60)
                                          CTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGC                                                                                                            >  SRR19973074.1501358/1‑150 (MQ=60)
                                                  GCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTC                                                                                                    <  SRR19973074.1079974/150‑1 (MQ=60)
                                                   CACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCC                                                                                                   >  SRR19973074.3464207/1‑150 (MQ=60)
                                                    ACGTTTAATTCTGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCT                                                                                                  <  SRR19973074.2287338/150‑1 (MQ=60)
                                                    ACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGTCACTCCT                                                                                                  <  SRR19973074.2864185/150‑1 (MQ=60)
                                                       TTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCT                                                                                               <  SRR19973074.724127/150‑1 (MQ=60)
                                                       TTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCT                                                                                               >  SRR19973074.2969614/1‑150 (MQ=60)
                                                       TTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCT                                                                                               <  SRR19973074.3855334/150‑1 (MQ=60)
                                                       TATAAGTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCT                                                                                               <  SRR19973074.724120/150‑1 (MQ=60)
                                                           ATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAC                                                                                           <  SRR19973074.2230254/150‑1 (MQ=60)
                                                           ATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAC                                                                                           >  SRR19973074.459383/1‑150 (MQ=60)
                                                           ATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAC                                                                                           <  SRR19973074.2230261/150‑1 (MQ=60)
                                                           ATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAC                                                                                           >  SRR19973074.196416/1‑150 (MQ=60)
                                                           ATTATGGGACGGCTGCTATCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATAC                                                                                           >  SRR19973074.459379/1‑150 (MQ=60)
                                                                GGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTC                                                                                      <  SRR19973074.2747932/150‑1 (MQ=60)
                                                                     GGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACT                                                                                 <  SRR19973074.3136036/150‑1 (MQ=60)
                                                                      GCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTT                                                                                >  SRR19973074.2787108/1‑150 (MQ=60)
                                                                        TGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCA                                                                              <  SRR19973074.1770328/150‑1 (MQ=60)
                                                                          CTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAG                                                                            >  SRR19973074.2380238/1‑150 (MQ=60)
                                                                           TTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGT                                                                           >  SRR19973074.1822234/1‑150 (MQ=60)
                                                                           TTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGT                                                                           >  SRR19973074.2329417/1‑150 (MQ=60)
                                                                              CTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGC                                                                        >  SRR19973074.3667241/1‑150 (MQ=60)
                                                                                TCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAT                                                                      >  SRR19973074.2023262/1‑150 (MQ=60)
                                                                                TCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCAT                                                                      <  SRR19973074.1234747/150‑1 (MQ=60)
                                                                                 CCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATG                                                                     <  SRR19973074.3804877/150‑1 (MQ=60)
                                                                                  CGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGT                                                                    >  SRR19973074.2141527/1‑150 (MQ=60)
                                                                                  CGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGT                                                                    >  SRR19973074.3054968/1‑150 (MQ=60)
                                                                                  CGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGT                                                                    >  SRR19973074.2605263/1‑150 (MQ=60)
                                                                                  CGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGT                                                                    >  SRR19973074.2605294/1‑150 (MQ=60)
                                                                                   GGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTG                                                                   >  SRR19973074.3531500/1‑150 (MQ=60)
                                                                                   GGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTG                                                                   >  SRR19973074.3531675/1‑150 (MQ=60)
                                                                                        TGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCG                                                              <  SRR19973074.162500/150‑1 (MQ=60)
                                                                                         GTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGG                                                             >  SRR19973074.557109/1‑150 (MQ=60)
                                                                                           CCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCT                                                           <  SRR19973074.1022124/150‑1 (MQ=60)
                                                                                             ATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGC                                                         <  SRR19973074.998531/150‑1 (MQ=60)
                                                                                                CGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATT                                                      <  SRR19973074.1147453/150‑1 (MQ=60)
                                                                                                 GTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTC                                                     <  SRR19973074.98232/150‑1 (MQ=60)
                                                                                                   GATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGT                                                   >  SRR19973074.2271367/1‑150 (MQ=60)
                                                                                                   GATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGT                                                   >  SRR19973074.3590304/1‑150 (MQ=60)
                                                                                                     TTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTC                                                 >  SRR19973074.1237966/1‑150 (MQ=60)
                                                                                                     TTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTC                                                 >  SRR19973074.2905672/1‑150 (MQ=60)
                                                                                                       ACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCAC                                               >  SRR19973074.2411787/1‑150 (MQ=60)
                                                                                                            GATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAG                                          >  SRR19973074.1320533/1‑150 (MQ=60)
                                                                                                            GATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGGTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAG                                          >  SRR19973074.559705/1‑150 (MQ=60)
                                                                                                             ATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGT                                         <  SRR19973074.146778/150‑1 (MQ=60)
                                                                                                             ATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGT                                         >  SRR19973074.2267312/1‑150 (MQ=60)
                                                                                                              TCTGGCTCGCCAAAGGTCATGCAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTC                                        >  SRR19973074.3329504/1‑150 (MQ=60)
                                                                                                               CTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCA                                       >  SRR19973074.3977208/1‑150 (MQ=60)
                                                                                                               CTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCA                                       >  SRR19973074.3977276/1‑150 (MQ=60)
                                                                                                               CTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCA                                       >  SRR19973074.3977327/1‑150 (MQ=60)
                                                                                                               CTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCA                                       <  SRR19973074.489009/150‑1 (MQ=60)
                                                                                                                  GCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTT                                    <  SRR19973074.2100995/150‑1 (MQ=60)
                                                                                                                    TCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTAC                                  >  SRR19973074.743877/1‑150 (MQ=60)
                                                                                                                      GCCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTT                                <  SRR19973074.408111/150‑1 (MQ=60)
                                                                                                                       CCAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTA                               >  SRR19973074.2184154/1‑150 (MQ=60)
                                                                                                                        CAAAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTAT                              >  SRR19973074.3047421/1‑150 (MQ=60)
                                                                                                                          AAGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGT                            >  SRR19973074.1100928/1‑150 (MQ=60)
                                                                                                                           AGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTA                           <  SRR19973074.1956917/150‑1 (MQ=60)
                                                                                                                           AGGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTA                           >  SRR19973074.601360/1‑150 (MQ=60)
                                                                                                                            GGTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAA                          >  SRR19973074.2850068/1‑150 (MQ=60)
                                                                                                                             GTCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAG                         >  SRR19973074.891748/1‑150 (MQ=60)
                                                                                                                              TCATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGC                        <  SRR19973074.2161110/150‑1 (MQ=60)
                                                                                                                                ATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTC                      >  SRR19973074.2668989/1‑150 (MQ=60)
                                                                                                                                ATACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTAATTATGTAAGCTC                      >  SRR19973074.2668961/1‑150 (MQ=60)
                                                                                                                                 TACAGCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCC                     <  SRR19973074.3550907/150‑1 (MQ=60)
                                                                                                                                     GCCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGG                 <  SRR19973074.2885105/150‑1 (MQ=60)
                                                                                                                                      CCGAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGG                <  SRR19973074.3700748/150‑1 (MQ=60)
                                                                                                                                        GAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCT              >  SRR19973074.3354778/1‑150 (MQ=60)
                                                                                                                                        GAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCT              <  SRR19973074.2719453/150‑1 (MQ=60)
                                                                                                                                        GAGGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCT              <  SRR19973074.2719472/150‑1 (MQ=60)
                                                                                                                                          GGCAAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTC            <  SRR19973074.3038418/150‑1 (MQ=60)
                                                                                                                                             AAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACT         <  SRR19973074.3045944/150‑1 (MQ=60)
                                                                                                                                             AAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACT         >  SRR19973074.4105534/1‑150 (MQ=60)
                                                                                                                                             AAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACT         <  SRR19973074.951511/150‑1 (MQ=60)
                                                                                                                                             AAAGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACT         >  SRR19973074.4105493/1‑150 (MQ=60)
                                                                                                                                               AGTCGGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGTTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCG       >  SRR19973074.2566371/1‑150 (MQ=60)
                                                                                                                                                   GGTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTG   >  SRR19973074.81161/1‑150 (MQ=60)
                                                                                                                                                    GTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGC  >  SRR19973074.3486374/1‑150 (MQ=60)
                                                                                                                                                    GTAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGC  <  SRR19973074.2375055/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGC  >  CP009273/433402‑433699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑