breseq  version 0.35.4  revision f352f80f4bc9 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	434,368	0	T	G	58.8%	3.6 / 14.1	18	W67G (TGG→GGG)	bioF	8‑amino‑7‑oxononanoate synthase
*	KT2440	381,781	0	A	C	58.3%	7.0 / 18.8	25	D201A (GAC→GCC)	soxB	Sarcosine oxidase subunit beta
*	KT2440	2,322,179	0	T	G	54.5%	6.5 / 18.0	22	V237G (GTG→GGG)	fadB_1	Fatty acid oxidation complex subunit alpha
*	KT2440	2,094,808	0	A	C	51.7%	‑3.5 / 10.2	30	L186R (CTG→CGG)	CJ019_01995	hypothetical protein
*	KT2440	3,138,367	0	T	G	31.7%	65.0 / 10.2	41	F240V (TTT→GTT)	rbsC_2	Ribose import permease protein RbsC
*	KT2440	6,039,837	0	C	A	30.5%	64.0 / 18.5	59	A37S (GCC→TCC)	puuD_3	Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
*	KT2440	4,218,062	0	T	A	25.0%	72.2 / 11.5	48	intergenic (‑547/+292)	CJ019_03957/CJ019_03958	hypothetical protein/hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	KT2440	= 5982606	15 (0.340)	3 (0.070)	3/236	4.7	13.6%	intergenic (+65/‑624)	kefC_3/CJ019_05604	Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
	?	KT2440	5982798 =	24 (0.580)					intergenic (+257/‑432)	kefC_3/CJ019_05604	Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
*	?	KT2440	= 2390060	40 (0.900)	3 (0.070)	3/238	4.8	6.3%	coding (2648/3630 nt)	bvgS	Virulence sensor protein BvgS
	?	KT2440	= 4525914	51 (1.210)					coding (1/1290 nt)	puuB_5	Gamma‑glutamylputrescine oxidoreductase