Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,830 T→G 21.2% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8300TG21.2% 197.7 / 11.0 98V109G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (19/54);  new base G (23/2);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 5.21e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGC  >  NC_000913/429598‑430057
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                    
ctgctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTggg                                                                                                                                                                                                                                <  2:29342/239‑1 (MQ=255)
 tgctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGc                                                                                                                                                                                                                               >  2:817000/1‑239 (MQ=255)
     tttgatgtctgtgtgcGTTTTTGTGGGCTTGTGGGGGTTTAACGGGGGGCTGGCTTTGAC‑CGGTGGTAAGGTGTTTGAAATTACGCTCGGAAAACCGGCTGAAATTGACGTAAGGCGGTATGACTTGCAAAAAGC‑C‑GGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGcc                                                                                                                                                                                                                           <  1:742656/224‑1 (MQ=255)
      tttcccggggttATCGTTTGTGTGGGCGCGCGCGATTTTACCGGGGGACTGGATTTCAC‑CGGTGGTACGGCTATAGAAATTCCGCTCGAAAAACCGGCTTAAATTGAGGAAAGGCGTGATTGATTGCAAAAAGC‑C‑CGTTTGGAAGCGCCGATGCTGCAAAACTTTGGTAGCAGCCATAACATCATGGTCCGTATGCCGCATGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCa                                                                                                                                                                                                                          <  1:660618/228‑1 (MQ=255)
      ttATTCGCTGTTTTCTTTTTGAGGGGCGGGGGCGGTTTAAACGGGGGGCTGGTTTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGAAAAAAGC‑C‑GGTTGTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCa                                                                                                                                                                                                                          <  1:292344/239‑1 (MQ=255)
       tAACCGTTGCTTTAGTTATTGTGGGCGGGGGCGGCTTTAACTGGGGGCTGGGTTTCAC‑CGGGGGTACGGTTATTGAAATTGCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCa                                                                                                                                                                                                                          <  2:870205/238‑1 (MQ=255)
             gtgcttgcGTTATTGTGGGCGTGTGCGGCTTTAACCGGGGGCTGGATTTCAC‑AGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTg                                                                                                                                                                                                                   <  2:557153/232‑1 (MQ=255)
              tgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGGTCTGa                                                                                                                                                                                                                  >  2:1031462/1‑239 (MQ=255)
                ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCCGGTTCTGAAg                                                                                                                                                                                                                >  2:984034/1‑239 (MQ=255)
                       tattatGGGCGTTCGCGGCTTTAACTGGGGGCTGGATTTCCC‑CGGTGGTACGTTTATTGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTa                                                                                                                                                                                                         <  2:597530/239‑1 (MQ=255)
                       tattatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGACTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGATGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGAtt                                                                                                                                                                                                          <  2:915799/238‑1 (MQ=255)
                        attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCTGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGGTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCTGCTGAAGGCTAACACGGGGGTCT‑AGGGGTGGGCAGCCCGGGCTTGAAGGTGTTTaa                                                                                                                                                                                                        >  1:102338/1‑239 (MQ=255)
                        attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGGGATTaa                                                                                                                                                                                                        >  2:1156211/1‑239 (MQ=255)
                         ttagGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAgg                                                                                                                                                                                                               <  2:1126868/231‑1 (MQ=255)
                                cGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGGCGCCAGGGTTTGAAGGGGATTAACGAAAcc                                                                                                                                                                                                 >  2:41051/1‑238 (MQ=255)
                                cGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCGGCCAGGTTCTGAAGGGGGTTAACGAATcc                                                                                                                                                                                                 >  2:1198808/1‑238 (MQ=255)
                                 gTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAACACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTGTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGCAGGCGGAACCGGCGGCCACGGGGTGGGGCACCCAGG‑TCTGaaaggggataacggagaca                                                                                                                                                                                                >  1:805547/1‑221 (MQ=255)
                                 gTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTCTGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTTGGCAGCCAGGTTCTGTAGGTGTTTTACGTATccc                                                                                                                                                                                                >  2:694135/1‑237 (MQ=255)
                                  tGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGCAACCGCCGGGCC‑GGGGTTTGGCGGCCAGGTTCTGAAAGGGATTAACGAAGccacc                                                                                                                                                                                              >  2:858576/1‑239 (MQ=255)
                                  tGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTTGGCAGCCAGGTTCTGAAGGTGGTTAACGAATcacac                                                                                                                                                                                              >  2:1130565/1‑235 (MQ=255)
                                   gcgcGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGGGGGCA‑GGGGTTTGGCAGGCAGGGTCttgagggggataaagaacagtt                                                                                                                                                                                               >  1:1126868/1‑216 (MQ=255)
                                   gcgcGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGAAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGGCC‑GGTGTTGGGCAGCCAGGTTCTCAAGGTGATTAACGAATcacacc                                                                                                                                                                                             >  2:973853/1‑234 (MQ=255)
                                    cgcgGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCA‑GAGGTTTGGCACACCGGTCTTGAAGGGGGTTAAAGAAAACACCaa                                                                                                                                                                                            >  1:293964/1‑239 (MQ=255)
                                     gcgGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGGTCTGAAGGTGATTAACGAATCCACCaa                                                                                                                                                                                            >  2:749752/1‑238 (MQ=255)
                                      gggttttAATGGGGGGATGGATTTCACGGGGTGATA‑GTGTATTTAAAGTCCCCTGGAAAAGCCGGCTTAAATTGACGTAAAGCTGGATGCATTGCAAAAAGC‑C‑GGTTTTGAATAGCCGATGGTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                           <  1:41051/234‑1 (MQ=255)
                                      ggggtttAAGTGGGGGGTGGATTTACC‑CGGTGGGAAGTTTATTGAAATTACGCTGGAAAAACCGGCTGAAATTGACGTAATGCGTGATGAATTGCAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                           <  1:817000/234‑1 (MQ=255)
                                            tAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGATGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                    <  2:23895/239‑1 (MQ=255)
                                                 gggggCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑TGGGTTGGGCAGCCAGGTTCTGAAGGTGAATAACGAATCCACCAATCAGAATgcagcc                                                                                                                                                                               >  2:897212/1‑238 (MQ=255)
                                                 gggggCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcagca                                                                                                                                                                               <  2:614132/239‑1 (MQ=255)
                                                   gggCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGGTCTGAAGGGGGTTAACGAATCCCCCAATCAGAATgcagccat                                                                                                                                                                             >  2:785518/1‑236 (MQ=255)
                                                   gggCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGcagcagg                                                                                                                                                                             >  2:374420/1‑238 (MQ=255)
                                                   gggCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GATTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCCGGTTCTGAAGGGGGTTAACGAATCCACCAATCAGCATggcacagg                                                                                                                                                                             >  2:1292509/1‑232 (MQ=255)
                                                    tgtttatcaggagcaggttgtACGGTTTTTGAAATTACGCTCAAAAAACCGGCTGAAATTGACGTAAGGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                             <  1:403864/224‑1 (MQ=255)
                                                    ggCTGGATTTCCC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                             <  2:844842/238‑1 (MQ=255)
                                                    ggCTGGATTTCCC‑CGGTGGTACGGTTATTGAAATTACGCTAGAAAAGCCGGCTGAAATTGACGTAATGCGTGATGCAGTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                             <  2:571522/238‑1 (MQ=255)
                                                      cTGGATTTCCC‑GGGTGTGACGGTTATGTAAATTTCGCTCAAAAACCCGGCTGAAATTGACGTAATGGGGGATGCCTTGCAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCAGGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                           <  1:778144/238‑1 (MQ=255)
                                                      cTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                           <  2:759439/238‑1 (MQ=255)
                                                      cTGGATTCCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                           <  2:744854/238‑1 (MQ=255)
                                                              cAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTg                                                                                                                                                                  >  2:596153/1‑239 (MQ=255)
                                                                      ggtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                           <  2:1123618/239‑1 (MQ=255)
                                                                      gggaagtttATTGAAATTAAGGTCGAAAAAGCGGCTGAAATGGTCGGATAGCGTGATGCATTGAAAAAAGC‑C‑GGTTTATAAGAGCCGATGTGGCAAAACTTTGGGAGCAGCCCTGACATCATGTGCCGTATGCTGCCTGCTGAAGGAGAATCCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGATTCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcg                                                                                                                                                            <  1:858576/231‑1 (MQ=255)
                                                                          cgtttAATGAAATTACGCTCGAAAACCCGGCTGAAATTGACGGAATGCGTGATGCTTTGAAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGt                                                                                                                                                       <  2:89666/236‑1 (MQ=255)
                                                                             tttttGAAATTAGCCTCCAAAACCCGTTGAAAATTGCCGTATGGCTTGAGTCATTGCAAAAAGC‑C‑GTTTTTGAAGAGCCGATGCTGCAAAACTTGGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                    <  1:485750/236‑1 (MQ=255)
                                                                              tATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGa                                                                                                                                                   >  2:280785/1‑239 (MQ=255)
                                                                               aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGa                                                                                                                                                                                                                  >  2:95314/1‑175 (MQ=255)
                                                                               aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAg                                                                                                                                                  >  2:256189/1‑239 (MQ=255)
                                                                               aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGGTCTGAAGGGGATTAACGAATCCACCAATCCGAATGCAGCAGGGAAGCGGATTgctgtctcttttaaaa                                                                                                                                                   >  2:403864/1‑223 (MQ=255)
                                                                               aTTGAAATTACGCTAGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGa                                                                                                                                                                                                                  <  1:95314/175‑1 (MQ=255)
                                                                                 tGAAATTGCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAGGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                <  2:827757/239‑1 (MQ=255)
                                                                                 tGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTTC‑GGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAAGATGCCGCAGGGAAGCGTATTGGTTTAGTCGGTCCGAACg                                                                                                                                                >  2:653447/1‑239 (MQ=255)
                                                                                  gAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt                                                                                                                                               <  2:735466/239‑1 (MQ=255)
                                                                                        acccgcGAAAAACCGGTTGAAATTGGCTGAATGGGGGATGCTTTGAAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCGGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                         <  1:596153/234‑1 (MQ=255)
                                                                                        aCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAACGTGGGGGc                                                                                                                                         >  2:1037897/1‑239 (MQ=255)
                                                                                         cGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGGTGGGCAGCCAGGTTCTGAAGGGGCTTAACGAATCCACCAATCAGAAGCCAGCAGGGAAGGGCATTGAGTTTTTGGGTCCGCGCGGGGGGGCa                                                                                                                                        >  1:701977/1‑239 (MQ=255)
                                                                                             ggAAAACCCGGCTGAAATTGCCGTAAGTCGTGAGGCA‑TGGAAAAAGCGC‑GGTTTTGAAGAGCCGATGTGGCAAACCTTTGGTAGCAGCCATGACATCGTGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                    <  1:280785/238‑1 (MQ=255)
                                                                                             ccaaaacCCGGCTGAAATTGACGTAATGCGTAATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                    <  2:693511/237‑1 (MQ=255)
                                                                                             cGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                    <  2:701977/239‑1 (MQ=255)
                                                                                             agAAAACCCGGGTAAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAACCTGTTGTGCAAAACTTTGGTAGGAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                    <  2:597677/238‑1 (MQ=255)
                                                                                               aaaaaCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGGGCTTAAAGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTGGTCGGTCCGAGCGTGGGGGCGGACCtt                                                                                                                                  >  2:1120557/1‑239 (MQ=255)
                                                                                                               tGATGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGa                                                                                                                  <  2:293964/239‑1 (MQ=255)
                                                                                                                 actgaaTGGGTGATGCATTGCAAAAAGC‑C‑GGTTTGAAAGGGCCGATGGTGCAAAACTGTGGTAGCAGCCATGATATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                <  2:602828/235‑1 (MQ=255)
                                                                                                                 aCGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGCGATTAACGAATCCACCAATCAGAATGCAGCCGTGAAGCGTATTGAGTTCGTCGGTCCGGGCGTGGGGGCAGACCTTGGCCAAACCCGTGCCAtt                                                                                                                >  2:1147261/1‑238 (MQ=255)
                                                                                                                            gATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATgg                                                                                                      <  2:265812/238‑1 (MQ=255)
                                                                                                                                   ttccaatggC‑C‑GGTTTCGAAGGGGTTTTGCTGAAACACGTGGGTAGCAGCAATGACATCATGGTCCGGATGCCGCCTGATGAAGGGTAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGAATAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTAGTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  1:485702/231‑1 (MQ=255)
                                                                                                                                   tGCAAAAAGC‑C‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  2:87372/239‑1 (MQ=255)
                                                                                                                                    tcAAAAAGC‑CTGGTTTTGAGGGGCCGATGCTGAAAAACTTGGGTAGCGGCCATGAAATCATGGCCCGTATGCCGCCTGGTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  1:256189/238‑1 (MQ=255)
                                                                                                                                     tggaaaagggctgtttgtaaGGGCGGATGCTGCAAAACTTTGGTAGTAGCCGGGTCCACAGGTGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTTTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  1:95790/221‑1 (MQ=255)
                                                                                                                                          tcc‑c‑GGTTTTGTGGGGCCGGTGTGGAAAAACGTTGTTAGCAGTCTTGAGTACAGGGTCCGTATGGCGCCCTCCGAAGGCGAACCCGGCGGGCA‑TGTTTTGGGGAGCCAGGGTCTGAAGGTGAGTAACGAATCCGCCAATCAGGATGGAGCGGTGAAGGGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGTCCTTGCGCACACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                       <  1:719054/237‑1 (MQ=255)
                                                                                                                                          aGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGGGTTGATGGCGGCGCTGCTGGCt                                                                                        >  2:1262887/1‑238 (MQ=255)
                                                                                                                                          aGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGCTGGGCAGCCAGGTTCTGAAGATGATTAACGAATCCACCAATCAGAATCCAGCAGTGAAACGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGGGCAAACAGGGGCGATGGCGTTGTTGGACGGGCGGCTGTCt                                                                                        >  2:525870/1‑238 (MQ=255)
                                                                                                                                              cgggcttggAAGAGCCGACTCTGCAAAACTTGGGTAGAAGTCATGACCTCATGGTCCGTATGCCGCCTGCTGAAGGCGACACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                       <  1:1130565/229‑1 (MQ=255)
                                                                                                                                              c‑GGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                     <  2:387407/238‑1 (MQ=255)
                                                                                                                                               ggtttttGAAGGCCCGATGCTGCAAACCTTTGGGAGCAGCCATGCCATCATGGGCCGTTGGCCGCTTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCGGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                     <  1:749752/235‑1 (MQ=255)
                                                                                                                                                 gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGC‑‑‑CA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCTTGGGGGCAGACCTTGCGCAAACGGGTCGGTGGGCGTTGTTGGAGGCGCTGCGGGCTACCCTC‑ggt                                                                              >  2:1030720/1‑237 (MQ=255)
                                                                                                                                                    ttGAAGGGCCGATGCTGTAAAACGTTGGTAACGGCCAGGACATCATGGTCCGTATGCCGCCTGGTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCGGCCAGGTTGTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑gt                                                                               <  1:979466/238‑1 (MQ=255)
                                                                                                                                                           gCCGATTCGTCAAAACTTTGGTGGCAGCCATGCAATCATGGGCCGGTTGCCGCCTGCTGAAGGCGAAACCGGCGGTAC‑GGGGTTGGGCAGCCAGGTTCTGGAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTa                                                                        <  1:1198808/238‑1 (MQ=255)
                                                                                                                                                            ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGGGCTGCTGCCTATCCTC‑GTGTACGAAgg                                                                      >  2:187631/1‑239 (MQ=255)
                                                                                                                                                            ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGGTGGGACGCCAGGTTCTTAAGGGGATTAACGGAACCCCCCATCCCAATGCAGCCGTGAAGCGGCTTGAGGTCGTCGGTCCCAGCGGGGGGGCAAACCTGGTGCAAACCGGGGAGCTGGGGTGGGTGGCGCCgctgtggccattccccgcgacacgagg                                                                       >  1:1118375/1‑216 (MQ=255)
                                                                                                                                                                         aCTGTGGTAGCATCCATGCCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAg                                                         <  2:1118375/239‑1 (MQ=255)
                                                                                                                                                                             tGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTTGGCAGCCAGGTTCTGAAGGTGATTAACGCATCCACCAATCAGAATGCAGCAGTGAAGCGTATTTAGTTCGTCGGTCCGAGCGTGTGGGCCGACCTTTCGCAAACCGGTGCGATGGCGTTGATGGTAGCGCTGCTGCCTATACTC‑GGTGCCGTAGGTTTCCGGCTTGAGTGGc                                                     >  2:1157962/1‑239 (MQ=255)
                                                                                                                                                                             tGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTGGATGGCAGGCGTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGc                                                     >  2:8362/1‑239 (MQ=255)
                                                                                                                                                                                tAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGa                                                   <  2:1192830/238‑1 (MQ=255)
                                                                                                                                                                                          tgcatcctGGGCCGTGTGCCGCCTGGATAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGGTCTGAAGGTGATTTACGAATTCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgg                                        <  1:785518/237‑1 (MQ=255)
                                                                                                                                                                                                    tCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGGCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGCGGGGACTGGCGGCCGGGGGGGTtttt                              >  2:1165707/1‑239 (MQ=255)
                                                                                                                                                                                                    tCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGGCTACCCCC‑GTGTACGTAGGTTTCCGCTTTGAGGGGGTCATGGGGGCAGgggggggtttt                              >  2:504220/1‑234 (MQ=255)
                                                                                                                                                                                                      cGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTgc                            >  2:746973/1‑239 (MQ=255)
                                                                                                                                                                                                       ttaggccgccttgtagaGGCCAACCCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGGCATTAACGAAGCCACCAATCAGAATGCAGCAGTGAATCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGGGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                           <  1:984034/223‑1 (MQ=255)
                                                                                                                                                                                                       gtttGCCGCCTTCTGAAGGCGAAACCGGGGGTCA‑GGTGTTGGGCAGCCGGGTTCTGAAGGTGATTACCGAATCCACCAATCCGAAGGCAGCAGTGAAGCGGTTGTAGTTCGTCGGTCCGGGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                           <  1:1031462/236‑1 (MQ=255)
                                                                                                                                                                                                        tATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGGGGTCGTCGGTCCGAGCGTGGGGGCAAACCTTGCGCAAAACCGTGCGATGGCGATGATGGCGGGGCTGCTGTGTATCCCC‑GTGTACGTGAGTTTCCGCGTTGGTGGGGGCCTGGCGGGCGGGGGGGGGATTgcgg                          >  1:290436/1‑238 (MQ=255)
                                                                                                                                                                                                        tATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCCGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCCGTGAAGCGTATTTCGTTCGGCGGTCCGAACGGGGGGGCAGGCCTTGCGCAAAACGGGGCGAGGGGATTGATTGGAGGGCTGCTGGTCATCCCCGGTGT‑CGGAGGTTTCACCTTTGCGGTGGGACTGGGGGCCGggggggggcttggcc                          >  1:1264283/1‑230 (MQ=255)
                                                                                                                                                                                                             cctctggggAAGGGGAAACGGGGGGTTG‑GGTGTTGGGCAGCCAGGTTTGGAAGGTGATTAACGAATCCACCAATCAGAATGGAGCCGTGAGGTGTATTTAGTTCGTTGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GGGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTAGTGCGCTGgcg                     <  1:1262887/231‑1 (MQ=255)
                                                                                                                                                                                                              cactggcaaaGGCGAAACCGGCGGTCA‑GGTTGGGGGCAGCCATGTTCTGAAGGTGATTACCGAATCCGCCAATCAGAATGCAGAGGTGAAGCGTATTGGGTTCGTCGGTACGGGCGTGGGGGCAGACCGTGCGCAAGCCGGTGCGGTGGCGTTGAGGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcgc                    <  1:1156211/231‑1 (MQ=255)
                                                                                                                                                                                                               ccTGCTGAAGGCGAAACCGGCGGTCA‑GGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGCGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTTGCGGCCGGGGTGGTTATTgcccctgggc                    >  2:672364/1‑230 (MQ=255)
                                                                                                                                                                                                                tcgcgaaggggtaaaCCGGGGGTCA‑GGTGTTGGGGAGGCAGGTTATGAGGGTGATTATCGGAGCCACCAATCAGAAAGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAGCCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCGGGCGCa                   <  1:1120557/226‑1 (MQ=255)
                                                                                                                                                                                                                     tcgtgaggcgaaacctgccA‑GGTGGTGGGCCGGCAGGTTCTGAAGGTGTCTAACGAATCCACGAAGCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTTCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGGCTATCCTC‑TTGTACGTAGGTTTCCGCTTTGACTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt             <  1:1030720/221‑1 (MQ=255)
                                                                                                                                                                                                                     gacggCGCAACCGGCGGTCA‑GGTTTTGGGCGGCCAGGTTCTGAAGGTGATTAACGAAGCCACAGATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCTTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCTACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt             <  1:504220/236‑1 (MQ=255)
                                                                                                                                                                                                                     gAAGGCGAAACCGGCGGTTG‑GGGTGTGGGCCGCCCGGTTGTGAAGGGGATTAACGAATCCAACAAGCAGAATGCAGCAGTGAAGCGTATTGGGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTTAGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt             <  1:1165707/239‑1 (MQ=255)
                                                                                                                                                                                                                                cggcggTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGc  <  2:141719/239‑1 (MQ=255)
                                                                                                                                                                                                                                           gtgtTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                           >  2:281856/1‑45 (MQ=255)
                                                                                                                                                                                                                                           gtgtTGGGCAGCCAGGTTCTGAAGGTGATTAACGAAGCCACCAAt                                                                                                                                                                                           <  1:281856/45‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                    
CTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAC‑CGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGC‑C‑GGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCA‑GGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTC‑GTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGC  >  NC_000913/429598‑430057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A