Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,830 T→G 15.4% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8300TG15.4% 315.1 / 7.7 132V109G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (32/70);  new base G (25/3);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 3.51e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429593‑430066
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
ttGGTCTGTTTTTTATCTGTGCTTGCGTTATTATGGGCGTGCGGGGCTTTAACTGGGGGCTGGATTTCCCCGGTG‑GGACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAAGGCGTGATGGATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGgtg                                                                                                                                                                                                                                               <  2:261410/238‑1 (MQ=255)
  tgTCTGTTTTTAAGCGCTTTTTTCGTTGTTATTGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATGGAAATTACGCACGAAAAACCGGCTGAAATTGACTTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGt‑t                                                                                                                                                                                                                                            <  2:406900/238‑1 (MQ=255)
     tgtgttttaatgcttgtttttgTTTTTATGGGGGGGGGGGGTTTTAACTGGGGGTTTGTTTTCCCCGGTG‑GTTCGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑Tggg                                                                                                                                                                                                                                         <  2:11165/218‑1 (MQ=255)
           tttaTCGCTTCTGTTTTTTTTATGGGCGTGGGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTTCGCTCGAAAAACCGGCGGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCa                                                                                                                                                                                                                                   <  2:1027139/236‑1 (MQ=255)
           ttAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCAGCGGTCAGGTGT‑TGGGCAGCCa                                                                                                                                                                                                                                   <  1:330292/239‑1 (MQ=255)
            tAATCGCTGCTATCGGTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCa                                                                                                                                                                                                                                   <  1:917240/238‑1 (MQ=255)
              aTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGGAGCCCggg                                                                                                                                                                                                                                >  1:323157/1‑238 (MQ=255)
                  ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAgggtttg                                                                                                                                                                                                                            >  1:1027139/1‑234 (MQ=255)
                  ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCATATGCCGCCT‑CCTGCAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAGGTTCtt                                                                                                                                                                                                                            >  1:1165024/1‑238 (MQ=255)
                     ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGGGAAACCGGCGGCCAGGGGTGTGGGC‑GCCAgggttggaag                                                                                                                                                                                                                         >  2:522954/1‑231 (MQ=255)
                     ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGGATGCCGCCT‑GCTTAAGGCGAAACCGGCGGTCCGGCGT‑TGGGCAGCCAAGTTCTGAAg                                                                                                                                                                                                                         >  1:455385/1‑239 (MQ=255)
                     ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCAGGTTTTGAAGAGCCAAGCCTGCAAAACTTTGGTAGCAGCACTGCAATCATGGGCCGTATG‑CGCCTGGCTGATGGCGAAACCGGCGGACAGGGGG‑Tggggaacacgggttgagaa                                                                                                                                                                                                                         >  2:278254/1‑223 (MQ=255)
                           tttttttGGGGGTGCGCGGCTTTAACTGGGGGCTGGATTTCCCCGGTG‑GTAAGGTTATTGAAATTACGCTCGAAAAACCGGCGGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGAtt                                                                                                                                                                                                                   <  2:768791/233‑1 (MQ=255)
                             attatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGGCCGGGGT‑TGGGCAGCCAgggttttgagggggtg                                                                                                                                                                                                                   >  2:109477/1‑223 (MQ=255)
                               tatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGTT‑TGGGCAGCCAGGTGTTGAAGGGGATTAACg                                                                                                                                                                                                               >  1:447594/1‑239 (MQ=255)
                                     cGTGGGCGGTTTTACTGGGGGGGTGGATTTCACGGGGG‑GTACGTTTATTGAAATTACGCTCGAAAAACCGGCGGAAATGGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcc                                                                                                                                                                                                          <  2:147485/238‑1 (MQ=255)
                                       tGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCACCCCGGTTCTGAAGGGGATTAACGAATccccc                                                                                                                                                                                                       >  1:112747/1‑239 (MQ=255)
                                        gcgcGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGGAACCCGGGTCTGAAAGGGAATaaaagaaacaaca                                                                                                                                                                                                      >  2:636025/1‑228 (MQ=255)
                                        gcgcGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGAAGCCATGACATCATGGTCCGAATGCCGCCT‑CCTGAGGGCGAAACCGGCGGGCAGGGTT‑TGGGAGGCCCGGTTCTGAAGGTGGTTaagagatccccc                                                                                                                                                                                                       >  1:1294938/1‑228 (MQ=255)
                                         cgcgGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGCCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGGTTAACGAATccacca                                                                                                                                                                                                      >  1:350379/1‑238 (MQ=255)
                                               tttAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGa                                                                                                                                                                                                <  1:522954/238‑1 (MQ=255)
                                                 tAACTGGGGGCTGGATTTCACCGGTG‑GTAAGGTTATTGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                             <  1:912269/239‑1 (MQ=255)
                                                    cTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcag                                                                                                                                                                                          <  1:854364/239‑1 (MQ=255)
                                                      gggggCGGAATTTCACCGGTG‑GTAACGTTATTGAAATTACGTCCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcagca                                                                                                                                                                                        <  1:1080675/239‑1 (MQ=255)
                                                        gggCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGGAGCCAGGTTTTGAAGGGGCTTAACGAATCCACCAATAAGAATgcagccgg                                                                                                                                                                                      >  1:1155822/1‑236 (MQ=255)
                                                        gggCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCGGAAGGCGAAACCGCCGGGCAGAGGG‑TGGGCAGCCCGGATCTTACGGGGAGTAAAGAAAccccacaaaaagattcagcagg                                                                                                                                                                                      >  2:1260292/1‑219 (MQ=255)
                                                         ggCTGGATTTCACTGGTGTGTA‑GGTTATTGAAATTCCGCTCGAAAAACCGGCTGAAAGTGACTTAATGCGTGATGCATTGCAAAAATCCGGTTTTGAAGAGCCGATGCTGCAAAACGTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                      <  2:1080792/238‑1 (MQ=255)
                                                         ggCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                      <  1:636025/238‑1 (MQ=255)
                                                         ggCTGGATTTCACCGGTG‑GTACGGTTATGGAAATTACGCTCGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCTGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                      <  1:591164/238‑1 (MQ=255)
                                                           ttGGATTTCCCCGGTG‑GGACGGTTATTGAAATTATGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGAGGCATTGCAAAAGGCCTGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                    <  1:1345623/237‑1 (MQ=255)
                                                           gtGGTTTTCCACGGTG‑GGTCGGTTATTGAAATTACGCCCGAAAAGCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGTTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                    <  2:89840/237‑1 (MQ=255)
                                                            tGGTTTTCCCCGGTG‑TTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGAAGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAg                                                                                                                                                                                  <  2:1001828/239‑1 (MQ=255)
                                                                        gtg‑gtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTTTTGAGTtc                                                                                                                                                                      >  1:228065/1‑239 (MQ=255)
                                                                            gtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                         <  1:958437/201‑1 (MQ=255)
                                                                               cGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGt                                                                                                                                                                <  1:374667/239‑1 (MQ=255)
                                                                                   tATTGAAATTACGCTGAAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                             <  1:527346/238‑1 (MQ=255)
                                                                                    aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCGGTGAAGCGGATTGAGTTCGTCGGTCCGa                                                                                                                                                            >  1:89499/1‑238 (MQ=255)
                                                                                    aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGGCGCCAGGTTTTGAAGGTGATTAAAGAAACCCCCAATCACAAATCAGCAGTGGAggcgattgggattgtcgggtcgag                                                                                                                                                           >  2:927830/1‑216 (MQ=255)
                                                                                      tGAAATTAGCCCTGAAAAACCTGCTGAAATTGGGGGAAGGCGTGAGGCATTGAAAAAAGGCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGAAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                         <  2:185247/239‑1 (MQ=255)
                                                                                       gAAATTTCCCTCAAAAACCCGTTAGAAATTGCCGTAAGGGGGTATGCTTTGAAAAAAGCGTGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt                                                                                                                                                        <  2:1155822/239‑1 (MQ=255)
                                                                                       gAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt                                                                                                                                                        <  1:1067597/239‑1 (MQ=255)
                                                                                       gAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt                                                                                                                                                        <  1:266963/239‑1 (MQ=255)
                                                                                        aaattcccgttaaaaaaCCGGGTGAAATTGGCTTAATGGGTGATGAATTGCAAAAAGGCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCCGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTg                                                                                                                                                       <  2:534588/227‑1 (MQ=255)
                                                                                             acgagagAAAAACCGGCTGAAATTGACGTAATGCGTGATTCATGGCAAAAAGCCGGGTTTGAAGAGCCGATGCTGCAAAGCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCTGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                  <  1:406150/233‑1 (MQ=255)
                                                                                                cTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCCGCCAGGTTCTTAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCGTTGACACGAATTTGGTTCTTCGGTCCGAGCGGGGGGGccg                                                                                                                                                >  1:534588/1‑236 (MQ=255)
                                                                                                cTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCCGAATGCGGCGGTGAAGGGGATTGAGTTCGTCGGTCCGAGGGTGGTGGCAg                                                                                                                                                >  1:390172/1‑238 (MQ=255)
                                                                                                  cGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                             <  1:407067/239‑1 (MQ=255)
                                                                                                       acccGGGTGAAATTGTCGGTATGCGTGATGCAGTGCAAAAAGGCTGTTGTGAAGAGCCGATCCTGCAAAACTTTGGTAGGAGCCATGGCTTCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTAAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTgcg                                                                                                                                        <  2:944194/237‑1 (MQ=255)
                                                                                                                    ggccGTATTGCGTGTTGGATTGAAAAAAGCGGGTTTTGAAGAGCCGTTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGa                                                                                                                           <  2:80500/236‑1 (MQ=255)
                                                                                                                      gcttaaggggttacgtaTTGAAAAAACGCTGTTTTGAGGGGGCGGTGGTGCAAACCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                         <  2:350379/223‑1 (MQ=255)
                                                                                                                      ctgtaagtggtggtggtttGAAAAAGGCCGGTTTTGAAGGGCCGATGCCGCAAACCGTGGGTAGCAGCCATGACAGCATGGTCCGTATGCCGGCT‑GCGGAAGGCGAAACCGTCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                         <  2:228065/222‑1 (MQ=255)
                                                                                                                      aCGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                         <  1:81930/239‑1 (MQ=255)
                                                                                                                       cGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGTCATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                               >  1:65942/1‑168 (MQ=255)
                                                                                                                       cGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGTCATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                               <  2:65942/168‑1 (MQ=255)
                                                                                                                           aTGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGGGTTCGTCGGTCCGAGCGGGGGGGGAGACCTTGCGCAAACCggggggaggggggg                                                                                                                    >  2:1170773/1‑227 (MQ=255)
                                                                                                                                  aTGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TAGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGAACTGAAGCGTATTAAGTTTGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGTTggga                                                                                                             >  1:116768/1‑237 (MQ=255)
                                                                                                                                   tGCATTGCAAAAAGCCGGTTTTTAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                            <  1:1345026/239‑1 (MQ=255)
                                                                                                                                       ttGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATTACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgct                                                                                                        <  1:1014849/239‑1 (MQ=255)
                                                                                                                                       ttGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                         <  1:221269/238‑1 (MQ=255)
                                                                                                                                        ttcAAAAAGCCGGTTTTGAAGGGCCGATTGTGCAAAACTTTTGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                       <  2:75142/237‑1 (MQ=255)
                                                                                                                                        tggaaaaaGCCGATTTTGAAAAGCCGGTGCTGCAAAACTTTGGTAGGAGCCAGGACATCAGGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGGAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                       <  1:776400/236‑1 (MQ=255)
                                                                                                                                        tGCAAAAGCCCTGTTTTAAACGGCCGGCGTGGCAAACTTTTGGTAGGCGCCATGACATCATGGTCCGTAGGCCGCCT‑GCTGAAGGCGAAACCGGGGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                       <  2:1372788/239‑1 (MQ=255)
                                                                                                                                        tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGa                                                                                                                                               >  1:1069835/1‑199 (MQ=255)
                                                                                                                                        tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                       <  1:828996/239‑1 (MQ=255)
                                                                                                                                        tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGGAGACCTTGCGCCAACCGGTGCCAGGGCGTTGGTGGGAgcgcgc                                                                                                       >  1:562247/1‑237 (MQ=255)
                                                                                                                                        tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGAGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAAGGAATCCACCAATCAGAATGCCGCCGTGAAGCGTATTGAGTTCGTCGGTTCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGAAGGccggggt                                                                                                        >  1:1134829/1‑232 (MQ=255)
                                                                                                                                        tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAAGTGT‑TGGGCAGCCAGGTTCTGTAGGGGATTAACGAATCCACCAAACAGAATGCAGACGTGAAACGTATTGAGTTCGTCGGTCCGAGCGTGGGGGGCGACCTGGCGCAAACCGGGAGGAAGGCGTTGATGGccaggcgg                                                                                                       >  2:904128/1‑232 (MQ=255)
                                                                                                                                          cAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCCCGTTT‑TAGGCACCCAGGGTCTGGCGGGGCTTAACGGACACACCAATCCGAATGCAACCGTGACACGTGTTGAGTTCGTCGGTCCAGGACTGGGGGCCGACCCTTCGCAAACCGGGGCCGTGGCGTTgtaggcgcggcgccg                                                                                                     >  2:104509/1‑225 (MQ=255)
                                                                                                                                             aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGGAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGCATTGGTTTCTTCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAAACGGTGCGATGGGGTTGATGGGAGGgctgcgg                                                                                                    >  1:741959/1‑235 (MQ=255)
                                                                                                                                             aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                         >  1:15139/1‑136 (MQ=255)
                                                                                                                                             aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                         <  2:15139/136‑1 (MQ=255)
                                                                                                                                             aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCCGCAGTGAAGCGTATTGAGTTCGTTGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGATTGTAGGgctgcgg                                                                                                    >  1:639274/1‑235 (MQ=255)
                                                                                                                                                  cGGTTTTGAAGGGCCGAGGCTGCAACACTTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                              <  2:323157/238‑1 (MQ=255)
                                                                                                                                                  aggTTTTGAAGGGCCGGTGCGGCAAAACTTTGGGAGCCGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATcc                                                                                             <  2:245305/238‑1 (MQ=255)
                                                                                                                                                           agggCCGGTTCTTCAAAATTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACg                                                                                    <  2:379159/236‑1 (MQ=255)
                                                                                                                                                           agagCCGATGTTGTAAAACTTTGTGAGCAGCCATGACATCATGGTCCGTATGCCGCCG‑GCTGAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACg                                                                                    <  2:288452/239‑1 (MQ=255)
                                                                                                                                                            gagCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                         <  1:752580/169‑1 (MQ=255)
                                                                                                                                                               ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGGGCTGCTGTCTATcccctgtcccttata                                                                                >  1:1375679/1‑226 (MQ=255)
                                                                                                                                                               ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGGACGTggg                                                                                >  1:162456/1‑239 (MQ=255)
                                                                                                                                                               ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCCATCAGAATGGAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGGTGATGGAGGCGCGCCGGGCTATCCTCGTGGACGGAgg                                                                                >  2:431906/1‑239 (MQ=255)
                                                                                                                                                                    gctgcAAAGCTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGa                                                                                                                                                                                                              <  1:593714/108‑1 (MQ=255)
                                                                                                                                                                    gctgcAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGa                                                                                                                                                                                                              >  2:593714/1‑108 (MQ=255)
                                                                                                                                                                                          cctggcatcatGGTCCGTAGGGCGCCT‑GCTGAAGGCGAAACCGGGGGTCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                      <  1:278254/233‑1 (MQ=255)
                                                                                                                                                                                           atggcttctttgttcgtcagccgcaT‑GGTGAAGGCGAAACCGGGGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                    <  2:1294938/220‑1 (MQ=255)
                                                                                                                                                                                               aatcatTGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                                <  1:927830/238‑1 (MQ=255)
                                                                                                                                                                                                   atGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGT‑GGGGCAGACCTTGCGCAAACCGGTGCGCTGGCGTTGATGGCAGGGCTGCTGTCCATCCTCGTGTACGTGGGTTTACGCTTTGAGTGGGGACTGGGGGCCGgggggggt                                           >  1:69086/1‑237 (MQ=255)
                                                                                                                                                                                                   atGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTCCGTAGGTTTCCGCTTTGCGGGGGGACTGGGGGCCGggggggg                                            >  1:1041499/1‑238 (MQ=255)
                                                                                                                                                                                                          gTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCCGACGGGGGGGGTAttcgc                                     >  1:702116/1‑236 (MQ=255)
                                                                                                                                                                                                           tATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGTTGGCAGCGCGGCTGCCTATCCTCCGGGACGAGGGTTTCCGCTTTGAGGGGCGGCGGGGGGCAGggggggggtattggt                                    >  2:73271/1‑230 (MQ=255)
                                                                                                                                                                                                           tATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                                <  2:912423/179‑1 (MQ=255)
                                                                                                                                                                                                           tATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                                >  1:912423/1‑179 (MQ=255)
                                                                                                                                                                                                                  cctgttgtaaGGCGAAACCGGGTGTCAGGTTT‑TGGGCGGCCAGGTTCTGAAGGTGATTAACGAACCCACCAATCCGAATGCAGGTGGGAGGCGTATTGGGTTTGTCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGgcgc                             <  2:447594/231‑1 (MQ=255)
                                                                                                                                                                                                                   ctcggtgAGGGCGAAACCGGCGGTCCGGTGT‑TGGGCAGGAGGGTTCTGAAGGTGGTTTATGGATCCACCAATCAGAATGCGGCAGTGAAGCGGATTGAGGTCGTTGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCGCGTGTACGTAGGGTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCa                            <  2:1234489/234‑1 (MQ=255)
                                                                                                                                                                                                                   cctggctaaGGCGAAACCGGGGGTCAGGTGG‑TGGGCAGCAAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCa                            <  1:481496/232‑1 (MQ=255)
                                                                                                                                                                                                                      gctgAAGGCGAAACCGGCGGTCAGGGGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGCCGGTCCCAGCGGGGGGGCAGACCTTCCGCCAACCGGGGCGATGGCGTTGATGGCAGCGCTGCTTGCTATACTCAGGTCCGGAGGTTTCCCCATTGCTGGGGTCCCGGCGGCAGGGGGGGGTCTTTGGCGTGGCGCacc                          >  2:40623/1‑238 (MQ=255)
                                                                                                                                                                                                                         gAAGGCGAAACCGGGGGTCAGGTGT‑GGGGCAGCCAGGTGCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGGCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGt                      <  2:889268/239‑1 (MQ=255)
                                                                                                                                                                                                                                     ggctgTCAGGGTT‑TGGGCAGCCAGGTTCGGAAGGTTATTCACGAATCCACCAATCAGAAGGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCt          <  1:1260292/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gggcatgtgt‑TGGGCAGCCAGGTTCGGAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGGTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCGGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCTgg        <  2:562247/232‑1 (MQ=255)
                                                                                                                                                                                                                                            aGGTGT‑TGGGCAGCCAGGTTCTGAGGGGGATTAACGAATCCACCAATCAGGATGCAGCAGTGAAGCGTATGGAGTTCGTCGGTCCGAGGGTAGGGGCAGTCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCTGGGTAtt   <  2:1134829/239‑1 (MQ=255)
                                                                                                                                                                                                                                              gtgt‑TGGGCAGCCAGGTTTTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGGGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCTGGGTAttt  <  2:112747/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
CTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTG‑GTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCT‑GCTGAAGGCGAAACCGGCGGTCAGGTGT‑TGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGC‑TGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429593‑430066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A