Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,970,032 |
(G)6→7 |
coding (1002/1047 nt) |
wzzE → |
Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,970,026 | 1 | . | G | 92.7%
| 132.0
/ 3.0
| 41 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (17/21); total (18/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA > NC_000913/3969888‑3970157
|
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:514873/1‑139 (MQ=255)
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:1517433/1‑139 (MQ=255)
cATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggg < 1:1498536/139‑1 (MQ=255)
tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTACGTACGCCGGAAGAACCGGTAAAACGCGATAGCC‑‑CGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGAt < 2:555836/132‑1 (MQ=255)
tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTACGTACGCCGGAAGAACCGGTAAAACGCGATAGCC‑‑CGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGAt > 1:555836/1‑132 (MQ=255)
gAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACGTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGc < 2:1020774/139‑1 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTAACGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTc > 2:1344310/1‑139 (MQ=255)
aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTa > 2:1383835/1‑139 (MQ=255)
ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccg > 1:274422/1‑139 (MQ=255)
gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccgc > 1:32668/1‑139 (MQ=255)
tCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGGGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccgccg < 1:660873/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 2:274422/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 2:163054/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:1517433/139‑1 (MQ=255)
cAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGaaa < 2:1162097/139‑1 (MQ=255)
aCCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaa < 1:1383835/139‑2 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAacac > 2:1293463/1‑139 (MQ=255)
cGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAacac > 1:752603/1‑139 (MQ=255)
tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGAt > 1:1120/1‑82 (MQ=255)
tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGAt < 2:1120/82‑1 (MQ=255)
cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGgt > 2:897096/1‑90 (MQ=255)
cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg > 1:588353/1‑70 (MQ=255)
cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg < 2:588353/70‑1 (MQ=255)
cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGTGGGCTGATCGGGGCTGgt < 1:897096/90‑1 (MQ=255)
agaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgc < 1:1293463/139‑1 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGc > 2:229391/1‑75 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGc < 1:229391/75‑1 (MQ=255)
aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 2:752603/139‑1 (MQ=255)
aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCTTTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt < 1:514873/139‑1 (MQ=255)
aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg < 1:1351704/139‑1 (MQ=255)
cgtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCTTTAACCCGCCGTTGCTCGAAGTAACAGCACTGCTGCGGTGAGCGCAAAGGCGATCGCCGCTTATTCGAAGAGAAt < 1:34405/139‑1 (MQ=255)
ccTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa > 2:64431/1‑131 (MQ=255)
ccTGATGATTATGCGGGGCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 1:64431/131‑1 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccgccg > 1:499470/4‑53 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:499470/50‑1 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg < 2:1003811/95‑1 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg > 1:1003811/4‑98 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac < 2:163637/128‑1 (MQ=255)
tgcggggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac > 1:163637/4‑131 (MQ=255)
ggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:974464/129‑1 (MQ=255)
ggCATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga > 2:974464/1‑129 (MQ=255)
cATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 2:52847/99‑1 (MQ=255)
cATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt > 1:52847/1‑99 (MQ=255)
cATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa > 1:676028/1‑123 (MQ=255)
cATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 2:676028/123‑1 (MQ=255)
cATTGTCGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa > 2:166871/1‑139 (MQ=255)
|
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA > NC_000913/3969888‑3970157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
