Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,032	(G)_6→7	coding (1002/1047 nt)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,026	1	.	G	97.1%	237.8 / ‑0.8	68	coding (996/1047 nt)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (35/31); total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA  >  NC_000913/3969888‑3970157

ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑c                                                                                                                                       >  2:1325380/1‑139 (MQ=255)

            cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                          <  2:17398/139‑1 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgg                                                                                                                       >  2:1773938/1‑139 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgg                                                                                                                       >  2:510772/1‑139 (MQ=255)

                 tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgggg                                                                                                                     >  2:512937/1‑139 (MQ=255)

                 tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCgggg                                                                                                                     >  2:29255/1‑139 (MQ=255)

                   aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCt                                                                                                                   >  1:338912/1‑139 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTc                                                                                                             >  2:466753/1‑139 (MQ=255)

                            ccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCa                                                                                                          <  1:29255/139‑1 (MQ=255)

                               aCCCTGGATCCGCGTTTTCAGACCTATCACTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTa                                                                                                       >  1:1166713/1‑139 (MQ=255)

                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                           >  2:239018/1‑139 (MQ=255)

                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                           >  1:227001/1‑139 (MQ=255)

                                            gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                          <  2:227001/139‑1 (MQ=255)

                                             ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                         <  1:1519250/139‑1 (MQ=255)

                                             ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                         <  2:338912/139‑1 (MQ=255)

                                             ataagggacagaTCGTTATTGGCGAACGCCGGAAGACCCGGTAAAACGCGATAGCCCACTTCGGGCCTTCCTGATGATTATGCGGGGCATTTT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                         <  1:98791/128‑1 (MQ=255)

                                                  aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                                    <  1:1325380/139‑1 (MQ=255)

                                                        aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                              >  1:1355507/1‑139 (MQ=255)

                                                        aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                              >  2:907514/1‑139 (MQ=255)

                                                        aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑gggg‑ggg                                                                                                                              <  1:600255/90‑2 (MQ=255)

                                                        aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑gggg‑ggg                                                                                                                              >  2:600255/1‑89 (MQ=255)

                                                              aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct                                                                        >  1:989281/1‑139 (MQ=255)

                                                                ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc                                                                      >  2:1037030/1‑139 (MQ=255)

                                                                   cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAc                                                                                                     <  1:644376/105‑1 (MQ=255)

                                                                   cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAc                                                                                                     >  2:644376/1‑105 (MQ=255)

                                                                   cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaacctgtctcttat                                                                   >  2:98791/1‑128 (MQ=255)

                                                                                  ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                    <  2:989281/139‑1 (MQ=255)

                                                                                  ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                    <  1:512937/139‑1 (MQ=255)

                                                                                     gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                 <  2:1166713/139‑1 (MQ=255)

                                                                                           cgcgATAGCCCACGTCATGCCTTCCTGATGATTATGTGGGGCATTGTGG‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGCTGCTCGAAACAACCACACTGCTGCGGTGAGCGTAAACGCGCTCGACGCttc                                           >  1:439635/1‑138 (MQ=255)

                                                                                               aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                          <  1:848991/56‑1 (MQ=255)

                                                                                               aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGAt                                                                                                                          >  2:848991/1‑56 (MQ=255)

                                                                                               aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                                       >  1:372481/1‑139 (MQ=255)

                                                                                               aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                                       <  1:1037030/139‑1 (MQ=255)

                                                                                                tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                             <  2:687386/100‑1 (MQ=255)

                                                                                                tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                             >  1:687386/1‑100 (MQ=255)

                                                                                                     cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                                         >  1:1504591/1‑131 (MQ=255)

                                                                                                     cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                                         <  2:1504591/131‑1 (MQ=255)

                                                                                                      aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                               >  2:848195/1‑92 (MQ=255)

                                                                                                      aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                               <  1:848195/92‑1 (MQ=255)

                                                                                                          cgtGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                            <  1:1773938/139‑1 (MQ=255)

                                                                                                          cgtGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                            >  2:1181279/1‑139 (MQ=255)

                                                                                                            tGCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                          >  2:1224611/1‑123 (MQ=255)

                                                                                                            tGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑GGGGTGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                          <  1:1224611/123‑1 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                          <  2:1080808/74‑1 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                          >  2:309330/1‑74 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                          >  1:1080808/1‑74 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                          <  1:309330/74‑1 (MQ=255)

                                                                                                               cTTCCTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                       <  1:1181279/139‑1 (MQ=255)

                                                                                                                  ccTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                             >  1:94096/1‑114 (MQ=255)

                                                                                                                  ccTGATGATTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                             <  2:94096/114‑1 (MQ=255)

                                                                                                                      atgatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact                >  2:413183/1‑139 (MQ=255)

                                                                                                                       tgatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg               <  2:372481/139‑1 (MQ=255)

                                                                                                                        gatTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga              <  1:907514/139‑1 (MQ=255)

                                                                                                                         atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGTAAAGGAGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac             <  1:239018/139‑1 (MQ=255)

                                                                                                                         atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac             >  2:1462504/1‑139 (MQ=255)

                                                                                                                         atTATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac             <  2:1355507/139‑1 (MQ=255)

                                                                                                                           tATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact            >  2:414364/1‑138 (MQ=255)

                                                                                                                           tATGCGGGGCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact            <  1:414364/138‑1 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                             >  1:1092591/4‑71 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                             <  2:1092591/68‑1 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                     >  1:351529/4‑79 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                     <  2:351529/76‑1 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                                         <  2:1696007/104‑1 (MQ=255)

                                                                                                                             tgcggggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                                         >  1:1696007/4‑107 (MQ=255)

                                                                                                                                  ggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCTCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGTCTGTATTTgg    >  2:488257/1‑139 (MQ=255)

                                                                                                                                  ggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                  >  2:6012/1‑125 (MQ=255)

                                                                                                                                  ggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                  <  1:6012/125‑1 (MQ=255)

                                                                                                                                  ggCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg    >  2:1652284/1‑139 (MQ=255)

                                                                                                                                   gCATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGt   <  1:1462504/139‑1 (MQ=255)

                                                                                                                                    cATTGT‑CGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa  >  1:878098/1‑139 (MQ=255)

GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA  >  NC_000913/3969888‑3970157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A