Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,970,032 |
(G)6→7 |
coding (1002/1047 nt) |
wzzE → |
Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,970,026 | 1 | . | G | 95.1%
| 135.4
/ ‑0.0
| 41 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (20/19); total (21/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 |
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGG > NC_000913/3969888‑3970155
|
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:251999/1‑139 (MQ=255)
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:382612/1‑139 (MQ=255)
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 1:48366/1‑139 (MQ=255)
ggCCATGTTAAACACCCTAAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:718698/1‑139 (MQ=255)
ccATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg < 2:48366/139‑1 (MQ=255)
ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggggg < 1:623920/139‑2 (MQ=255)
ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggggg < 1:382612/139‑2 (MQ=255)
cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTCTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGAt < 1:782117/139‑1 (MQ=255)
cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCggg > 1:121477/1‑139 (MQ=255)
tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCgggg > 1:1210293/1‑139 (MQ=255)
tGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGgtgt < 1:718698/139‑1 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTc > 1:901104/1‑139 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggggctga > 1:40896/1‑120 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggggctga < 2:40896/125‑6 (MQ=255)
cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggggg > 2:1091272/1‑113 (MQ=255)
cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggggg < 1:1091272/114‑2 (MQ=255)
gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg > 2:292376/1‑139 (MQ=255)
cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 2:673928/1‑139 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:251999/139‑1 (MQ=255)
tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:292376/139‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGc < 1:311314/101‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGc > 2:311314/1‑101 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 1:620748/130‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 2:620748/1‑130 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 2:1006010/1‑139 (MQ=255)
cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt < 2:121477/139‑1 (MQ=255)
aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 2:1102690/1‑117 (MQ=255)
aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:1102690/117‑1 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggg > 1:714831/1‑63 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggg < 2:257002/63‑1 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggg > 1:257002/1‑63 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggggg < 2:714831/63‑1 (MQ=255)
aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 2:1210293/139‑1 (MQ=255)
cgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:901104/139‑1 (MQ=255)
tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccgcc < 2:591630/69‑1 (MQ=255)
tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccgcc > 1:591630/1‑69 (MQ=255)
gCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGGGGACTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 1:861092/1‑75 (MQ=255)
gCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGGGGACTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 2:861092/75‑1 (MQ=255)
ccTTCCTGTTGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:1002169/68‑1 (MQ=255)
ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccgccg > 1:1002169/1‑68 (MQ=255)
cTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTTTTCGAAGAGAATCGATGTGa < 1:673928/139‑1 (MQ=255)
cTTCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa < 1:612089/139‑1 (MQ=255)
tCCTGATGATTATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGaaa > 2:424782/1‑139 (MQ=255)
ttATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact > 2:556106/1‑139 (MQ=255)
tATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt > 1:643069/1‑108 (MQ=255)
tATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 2:643069/108‑1 (MQ=255)
tATGCGGGGCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg > 2:295483/1‑139 (MQ=255)
tgcggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTCACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg > 2:917771/4‑133 (MQ=255)
tgcggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:357637/4‑71 (MQ=255)
tgcggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:357637/68‑1 (MQ=255)
tgcggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg < 1:917771/130‑1 (MQ=255)
ggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt > 1:1218371/1‑114 (MQ=255)
ggggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt < 2:1218371/114‑1 (MQ=255)
ggCATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCAATAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGTCGAGAATCGATGTGAAAGTACTGACTGTATTTgg > 2:868396/1‑139 (MQ=255)
cATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 2:833973/1‑62 (MQ=255)
cATTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:833973/62‑1 (MQ=255)
|
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGG > NC_000913/3969888‑3970155
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
