Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,970,032 |
(G)6→7 |
coding (1002/1047 nt) |
wzzE → |
Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,970,026 | 1 | . | G | 98.3%
| 206.7
/ ‑5.6
| 59 | V332V (GTC→GTG) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (27/31); minor base A (0/1); total (27/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA > NC_000913/3969888‑3970157
|
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:1393547/1‑139 (MQ=255)
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 1:241092/1‑139 (MQ=255)
ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg < 1:987759/139‑2 (MQ=255)
ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgg > 1:1604765/1‑139 (MQ=255)
aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCt > 1:356246/1‑139 (MQ=255)
tGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt < 1:1504243/139‑1 (MQ=255)
ccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCa < 1:871608/139‑1 (MQ=255)
cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCAGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc < 1:335016/128‑1 (MQ=255)
cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCAGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc > 2:335016/1‑128 (MQ=255)
cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTa > 1:835464/1‑138 (MQ=255)
cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTa < 2:835464/138‑1 (MQ=255)
gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgc > 1:746952/1‑139 (MQ=255)
ccGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgccg < 1:1393547/139‑1 (MQ=255)
gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGACGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg > 2:226938/1‑139 (MQ=255)
cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgt < 2:368121/118‑1 (MQ=255)
cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgt > 1:368121/1‑118 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:1604765/139‑1 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:698767/139‑1 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:1271746/139‑1 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCCGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCA‑GGGGGCCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:1133086/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:162054/139‑1 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg < 2:272268/89‑2 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg > 1:272268/1‑88 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑g < 2:1582836/83‑1 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑g > 1:1582836/1‑83 (MQ=255)
cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:79815/126‑1 (MQ=255)
cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:79815/1‑126 (MQ=255)
cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgc > 1:1089611/1‑139 (MQ=255)
ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑gggggggc < 2:107385/74‑3 (MQ=255)
ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑gggggggc > 1:107385/1‑72 (MQ=255)
aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg > 1:854104/1‑139 (MQ=255)
aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg > 1:170767/1‑139 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt > 2:35183/1‑139 (MQ=255)
ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg > 1:683834/1‑139 (MQ=255)
tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:867046/98‑1 (MQ=255)
tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:867046/1‑98 (MQ=255)
aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 2:858179/99‑1 (MQ=255)
aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 1:858179/1‑99 (MQ=255)
aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg > 1:125193/1‑139 (MQ=255)
aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa < 2:241092/139‑1 (MQ=255)
gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa > 2:298985/1‑137 (MQ=255)
gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa < 1:298985/137‑1 (MQ=255)
gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:732673/1‑75 (MQ=255)
gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:732673/75‑1 (MQ=255)
ccTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg > 1:103766/1‑139 (MQ=255)
tgatgaTTATGCGGGGCATTGCCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 2:1089611/139‑1 (MQ=255)
gatgatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac < 2:746952/139‑1 (MQ=255)
gatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 2:125193/139‑1 (MQ=255)
gatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:226938/139‑1 (MQ=255)
atTATGCGGGGCATTGTCG‑GGGGGGTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:103766/139‑1 (MQ=255)
atTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:170767/139‑1 (MQ=255)
atTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:854104/139‑1 (MQ=255)
ttATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact < 1:777263/139‑1 (MQ=255)
tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt < 1:778136/40‑1 (MQ=255)
tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt > 2:778136/1‑40 (MQ=255)
tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg > 2:195778/1‑139 (MQ=255)
tgcggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 1:1204999/4‑59 (MQ=255)
tgcggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 2:1204999/56‑1 (MQ=255)
tgcggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:6783/68‑1 (MQ=255)
tgcggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:6783/4‑71 (MQ=255)
ggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 1:950337/68‑1 (MQ=255)
ggggCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 2:950337/1‑68 (MQ=255)
gCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAATTAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt > 2:1118107/1‑100 (MQ=255)
gCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 1:1118107/100‑1 (MQ=255)
gCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:831236/128‑1 (MQ=255)
gCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga > 2:831236/1‑128 (MQ=255)
aTTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa > 1:1472562/1‑138 (MQ=255)
aTTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa < 2:1472562/138‑1 (MQ=255)
|
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTA > NC_000913/3969888‑3970157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
