Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,970,032 |
(G)6→7 |
coding (1002/1047 nt) |
wzzE → |
Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,970,026 | 1 | . | G | 98.0%
| 172.9
/ ‑3.3
| 51 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (25/25); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 |
CCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969903‑3970146
|
ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgg > 2:823350/1‑139 (MQ=255)
tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgggg < 2:195742/139‑1 (MQ=255)
tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgggg > 1:543576/1‑139 (MQ=255)
gAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGc < 1:628892/139‑1 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTc > 2:979564/1‑139 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTc > 1:779028/1‑139 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑g < 2:15399/118‑1 (MQ=255)
ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑g > 1:15399/1‑118 (MQ=255)
cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCAt > 2:912593/1‑139 (MQ=255)
aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gggg > 1:689301/1‑114 (MQ=255)
aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gggg < 2:689301/115‑2 (MQ=255)
ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccg > 1:204296/1‑139 (MQ=255)
ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccg > 1:470184/1‑139 (MQ=255)
gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gg > 2:494797/1‑102 (MQ=255)
gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gg < 1:494797/102‑1 (MQ=255)
cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 2:504925/1‑139 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:504925/139‑1 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:3003/139‑1 (MQ=255)
gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:779028/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:912593/139‑1 (MQ=255)
ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:491167/139‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAAcc < 1:273075/118‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAAcc > 2:273075/1‑118 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 2:781763/1‑130 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 1:781763/130‑1 (MQ=255)
aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac > 2:325684/1‑139 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 1:400601/139‑1 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:543576/139‑1 (MQ=255)
tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 1:823350/139‑1 (MQ=255)
tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc > 2:753192/1‑139 (MQ=255)
ggAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCaa < 1:979564/139‑1 (MQ=255)
gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGgtg > 1:435788/1‑86 (MQ=255)
gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGgtg < 2:435788/86‑1 (MQ=255)
ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt < 2:470184/139‑1 (MQ=255)
tAAAACGCGATAGCCCACGTCGTGCCTTCTTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:868593/110‑1 (MQ=255)
tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:868593/1‑110 (MQ=255)
aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt < 2:204296/139‑1 (MQ=255)
cccACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct > 1:351115/1‑102 (MQ=255)
cccACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct < 2:351115/102‑1 (MQ=255)
cgtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACCGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt < 1:715294/139‑1 (MQ=255)
tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg < 1:325684/139‑1 (MQ=255)
gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt > 2:436269/1‑139 (MQ=255)
ccTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGGTAATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACGGCTGTGGTGATCGCAAAGGCGCTCGTCGCTTATTCGAAGAGAATCGATGGGAAAg > 1:687588/1‑139 (MQ=255)
ccTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAg > 1:267400/1‑139 (MQ=255)
atgatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact > 2:273289/1‑139 (MQ=255)
atgatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact > 2:168700/1‑139 (MQ=255)
gatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:436269/139‑1 (MQ=255)
tttATGCGGTGCATTGTCG‑GGG‑GGGCTCATCGGGGCTGGTGTCGCCTTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGCCGCTCGCCTCTTATTCGAAGAGAATCGATGTGAAAGCactgac < 1:574924/138‑1 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:868349/66‑1 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 2:868349/4‑69 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg > 1:660029/4‑79 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 1:20185/98‑1 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg > 2:20185/4‑101 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa > 1:54569/4‑120 (MQ=255)
tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:54569/117‑1 (MQ=255)
tgcggggCATTGTC‑‑GGGTGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg < 2:660029/76‑1 (MQ=255)
cATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg < 2:954794/126‑1 (MQ=255)
cATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg > 1:954794/1‑126 (MQ=255)
|
CCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969903‑3970146
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
