Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,532	T→C	intergenic (‑114/‑25)	queE ← / → yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,532	0	T	C	100.0%	144.4 / NA	41	intergenic (‑114/‑25)	queE/yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/20); total (21/20)

CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATA  >  NC_000913/2905398‑2905654

ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                        >  2:1191860/1‑139 (MQ=255)

ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                                          <  2:800053/137‑1 (MQ=255)

ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                                          >  1:800053/1‑137 (MQ=255)

      aaTCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                  <  2:1196000/139‑1 (MQ=255)

        tCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                <  2:1586049/139‑1 (MQ=255)

        tCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                <  2:778766/139‑1 (MQ=255)

                  cATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGta                                                                                                      >  2:2258/1‑139 (MQ=255)

                          tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCa                                                                                              >  2:952984/1‑139 (MQ=255)

                          tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCa                                                                                              >  2:486092/1‑139 (MQ=255)

                            tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                <  1:845735/119‑1 (MQ=255)

                            tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                >  2:845735/1‑119 (MQ=255)

                            tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCaga                                                                                            <  1:1534340/139‑1 (MQ=255)

                               gtgAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCagaaga                                                                                         <  1:2258/139‑1 (MQ=255)

                                 gAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGaaa                                                                                       >  2:1494754/1‑139 (MQ=255)

                                  aaGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATAATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGaaaa                                                                                      >  2:602390/1‑139 (MQ=255)

                                           aTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                                             <  1:871381/139‑1 (MQ=255)

                                                      ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                                          >  1:847098/1‑83 (MQ=255)

                                                      ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa                                                                                                                          <  2:847098/83‑1 (MQ=255)

                                                       tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                  >  2:1404339/1‑90 (MQ=255)

                                                       tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                  <  1:1404339/90‑1 (MQ=255)

                                                       tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                              >  2:71669/1‑126 (MQ=255)

                                                       tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                              <  1:71669/126‑1 (MQ=255)

                                                         cAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATg                                                               >  2:363850/1‑139 (MQ=255)

                                                            aTCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCa                                                            <  1:952984/139‑1 (MQ=255)

                                                            aTCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCa                                                            <  1:486092/139‑1 (MQ=255)

                                                                 gCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAACATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                <  1:1105241/82‑1 (MQ=255)

                                                                 gCCACAACAATCATGGCTTATTAACATTCTGTTACAGTCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                >  2:1105241/1‑82 (MQ=255)

                                                                          aTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCaaa                                              >  2:1465435/1‑139 (MQ=255)

                                                                           tCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATGATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCaaaa                                             >  1:877685/1‑139 (MQ=255)

                                                                           tCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCaaaa                                             <  1:363850/139‑1 (MQ=255)

                                                                              tGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat                                                                                                     >  2:997936/1‑80 (MQ=255)

                                                                              tGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat                                                                                                     <  1:997936/80‑1 (MQ=255)

                                                                                    attaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTAtattta                                    >  2:1643931/1‑139 (MQ=255)

                                                                                     ttaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAt                                                                        <  2:211858/102‑1 (MQ=255)

                                                                                     ttaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAt                                                                        >  1:211858/1‑102 (MQ=255)

                                                                                     ttaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                   >  1:86677/1‑107 (MQ=255)

                                                                                     ttaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                   <  2:86677/107‑1 (MQ=255)

                                                                                          aTTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTAtttttt                              <  1:1465435/139‑1 (MQ=255)

                                                                                            tCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCt                            >  1:20698/1‑139 (MQ=255)

                                                                                              tGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAATTTATATTTATTTTTTCTGt                          >  2:1084087/1‑139 (MQ=255)

                                                                                                       caggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGtttt                 <  1:1494754/139‑1 (MQ=255)

                                                                                                        aggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat                                                                                                     <  2:1128958/54‑1 (MQ=255)

                                                                                                        aggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat                                                                                                     >  1:1128958/1‑54 (MQ=255)

                                                                                                                    aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGaa    >  2:562885/1‑139 (MQ=255)

                                                                                                                     aaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAat   >  1:888359/1‑139 (MQ=255)

                                                                                                                      aTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAata  <  1:562885/139‑1 (MQ=255)

CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATA  >  NC_000913/2905398‑2905654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A