Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,905,532 |
T→C |
intergenic (‑114/‑25) |
queE ← / → yqcG |
7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,905,532 | 0 | T | C | 100.0%
| 124.4
/ NA
| 36 | intergenic (‑114/‑25) | queE/yqcG | 7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17) |
CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATA > NC_000913/2905398‑2905654
|
ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata > 2:720573/1‑139 (MQ=255)
ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata > 2:1118355/1‑139 (MQ=255)
gTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAAtt < 2:153021/139‑1 (MQ=255)
aaTCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa < 1:1118355/139‑1 (MQ=255)
tCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg < 1:783488/139‑1 (MQ=255)
aGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtatta > 2:363662/1‑139 (MQ=255)
gCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtattat > 1:571901/1‑139 (MQ=255)
tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAAtt < 2:589523/114‑1 (MQ=255)
tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAAtt > 1:589523/1‑114 (MQ=255)
tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg > 2:515719/1‑119 (MQ=255)
tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg < 1:515719/119‑1 (MQ=255)
aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg < 2:985044/98‑1 (MQ=255)
aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg > 1:985044/1‑98 (MQ=255)
aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt > 2:835746/1‑102 (MQ=255)
aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt < 1:835746/102‑1 (MQ=255)
attattGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAttt > 1:50488/1‑139 (MQ=255)
ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa < 2:1027290/83‑1 (MQ=255)
ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaa > 1:1027290/1‑83 (MQ=255)
tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaataa < 1:552277/85‑1 (MQ=255)
tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaataa > 2:552277/1‑85 (MQ=255)
ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa > 2:1159261/1‑120 (MQ=255)
ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa < 1:1159261/120‑1 (MQ=255)
ccTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCaaa > 2:1124848/1‑139 (MQ=255)
aacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa > 1:973661/1‑83 (MQ=255)
aacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa < 2:973661/83‑1 (MQ=255)
tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa > 2:985881/1‑64 (MQ=255)
tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa < 1:985881/64‑1 (MQ=255)
ttattaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTAtatt < 1:363662/139‑1 (MQ=255)
attaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa > 1:828123/1‑69 (MQ=255)
attaACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa < 2:828123/69‑1 (MQ=255)
tCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt < 2:336907/100‑1 (MQ=255)
tCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt > 1:336907/1‑100 (MQ=255)
gTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTa > 1:431480/1‑139 (MQ=255)
ggcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGtt > 1:408500/1‑139 (MQ=255)
gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa > 2:195260/1‑47 (MQ=255)
gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa < 1:195260/47‑1 (MQ=255)
gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACAAAAATTATATTTATTTTTTCTGTATTAGTTTTTaa > 2:131896/1‑139 (MQ=255)
gAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAAc < 1:1124848/139‑1 (MQ=255)
aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat < 2:501869/42‑1 (MQ=255)
aaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtat > 1:501869/1‑42 (MQ=255)
aaTGCGAAACATATCGTCAATAATTAACGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTCTTTTTTCTGTATTAGTTTTTAATGATAACGAat > 1:992558/1‑139 (MQ=255)
aTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAata < 1:131896/139‑1 (MQ=255)
|
CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATA > NC_000913/2905398‑2905654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
