Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F64 I0 R1 334 49.4 1968632 94.1% 1852482 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,019,993 Δ1 bp 73.4% coding (107/702 nt) fre → NAD(P)H‑flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,019,9930A.73.4% 131.9 / 80.1 64coding (107/702 nt)freNAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand):  ref base A (6/10);  new base . (20/27);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAC  >  CP009273/4019859‑4020123
                                                                                                                                      |                                                                                                                                  
cTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGa                                                                                                                               <  1:220088/140‑1 (MQ=255)
 tATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATg                                                                                                                             <  2:10612/140‑1 (MQ=255)
 tATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATg                                                                                                                             <  2:42960/140‑1 (MQ=255)
   tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATgg                                                                                                                            >  2:971878/1‑140 (MQ=255)
   tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  1:762997/140‑1 (MQ=255)
   tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  2:638812/140‑1 (MQ=255)
   tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  2:638709/140‑1 (MQ=255)
   tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  2:380344/140‑1 (MQ=255)
   ttgaTCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  2:874462/137‑1 (MQ=255)
           tCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTgatgg                                                                                                                   >  2:365064/1‑140 (MQ=255)
                       aGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACa                                                                                                       <  2:19098/140‑1 (MQ=255)
                       aGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACa                                                                                                       <  2:499120/140‑1 (MQ=255)
                                   ccTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGTTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTtct                                                                                           >  2:620200/1‑140 (MQ=255)
                                             tAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTc                                                                                 >  1:474886/1‑140 (MQ=255)
                                                     ccTCGGTATAAGCTATCACGGATACCGTATATCGTGTCCGCATGGTGCCAGACGCGGCCTTTTCTGTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTGCGTTCTCAATGGCTTCGACGCCgg                                                                         <  1:198039/140‑1 (MQ=255)
                                                     ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  1:777659/140‑1 (MQ=255)
                                                     ccTCGGTAGAAGCTATAACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTATTTTCGTGAGGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  1:386171/140‑1 (MQ=255)
                                                      cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGCTGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  1:953892/140‑1 (MQ=255)
                                                      cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  2:246816/140‑1 (MQ=255)
                                                      cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  2:217065/140‑1 (MQ=255)
                                                      cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                         <  2:645994/140‑1 (MQ=255)
                                                            aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaaa                                                                  <  2:188448/140‑1 (MQ=255)
                                                               aGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAgg                                                               <  2:221133/140‑1 (MQ=255)
                                                               aGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAgg                                                               <  2:469698/140‑1 (MQ=255)
                                                                   aTCACGGATACCGTATTTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGtt                                                            >  1:972352/1‑140 (MQ=255)
                                                                   aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGtt                                                            >  1:496273/1‑140 (MQ=255)
                                                                   aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGttt                                                           >  1:534165/1‑140 (MQ=255)
                                                                   aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGttt                                                           >  1:879689/1‑140 (MQ=255)
                                                                     cACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTa                                                          >  1:306882/1‑140 (MQ=255)
                                                                     cACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTa                                                          >  1:483973/1‑140 (MQ=255)
                                                                         gATACCGTATCTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAg                                                     <  1:699264/140‑1 (MQ=255)
                                                                         gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTGTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAg                                                     <  1:699265/140‑1 (MQ=255)
                                                                           tACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGc                                                    <  1:518569/140‑1 (MQ=255)
                                                                           tACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   <  2:439331/140‑1 (MQ=255)
                                                                           gaCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAGGGGTTTATCGAGCt                                                   <  2:539580/139‑1 (MQ=255)
                                                                            aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   <  2:447209/140‑1 (MQ=255)
                                                                            aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   <  2:310279/140‑1 (MQ=255)
                                                                            aCCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGAAAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   <  2:447217/140‑1 (MQ=255)
                                                                                tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                              >  1:105439/1‑140 (MQ=255)
                                                                                tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                              >  1:771167/1‑140 (MQ=255)
                                                                                tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                              >  1:814197/1‑140 (MQ=255)
                                                                                tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAG‑GTTTGATGGTAGTGATGGATTAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAATGTTTTATCGAGCTGCata                                              >  1:817063/1‑140 (MQ=255)
                                                                                     cGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgc                                         >  2:978191/1‑140 (MQ=255)
                                                                                     cGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgc                                         >  2:628532/1‑140 (MQ=255)
                                                                                        gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCt                                      >  1:345312/1‑140 (MQ=255)
                                                                                        gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCt                                      >  2:821824/1‑140 (MQ=255)
                                                                                         tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           >  1:218243/1‑54 (MQ=255)
                                                                                         tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                           <  2:218243/54‑1 (MQ=255)
                                                                                          ccGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGCGCTGCATATTGGCGCTTc                                    <  1:270879/140‑1 (MQ=255)
                                                                                          ccGCATCGGGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGGGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCtt                                     <  1:68616/140‑1 (MQ=255)
                                                                                                 gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATc                             >  2:954665/1‑140 (MQ=255)
                                                                                                 gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATc                             >  2:877624/1‑140 (MQ=255)
                                                                                                             gCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                    <  2:62401/121‑1 (MQ=255)
                                                                                                             gCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                    >  1:62401/1‑121 (MQ=255)
                                                                                                                 tttcttttcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg             <  1:954665/140‑1 (MQ=255)
                                                                                                                 tttcttttcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATAGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGaaat             <  1:877624/140‑2 (MQ=255)
                                                                                                                      tttcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc        >  2:765554/1‑140 (MQ=255)
                                                                                                                      tttcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTCCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc        >  2:131517/1‑140 (MQ=255)
                                                                                                                        tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa       >  1:819148/1‑140 (MQ=255)
                                                                                                                        tcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACTCCGGATGaaa                                                                   >  2:727441/1‑79 (MQ=255)
                                                                                                                        tcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACTCCGGATGaaa                                                                   <  1:727441/79‑1 (MQ=255)
                                                                                                                        tcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   <  2:793916/95‑1 (MQ=255)
                                                                                                                        tcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                   >  1:793916/1‑95 (MQ=255)
                                                                                                                            gCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAc  <  2:474886/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAC  >  CP009273/4019859‑4020123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A