Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F64 I0 R1	409	63.1	2344228	95.5%	2238737	135.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,019,993	Δ1 bp	75.7%	coding (107/702 nt)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,019,993	0	A	.	75.7%	164.0 / 81.9	70	coding (107/702 nt)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base A (14/3); new base . (25/28); total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCA  >  CP009273/4019857‑4020127

cccTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGttttg                                                                                                                                     >  1:809578/1‑139 (MQ=255)

 ccTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGa                                                                                                                                   <  1:1101061/140‑1 (MQ=255)

     tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  2:691678/140‑1 (MQ=255)

     tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  2:776617/140‑1 (MQ=255)

     tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  1:742886/140‑1 (MQ=255)

     tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  1:680496/140‑1 (MQ=255)

     tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  2:177075/140‑1 (MQ=255)

                               tGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTc                                                                                                     <  2:282152/140‑1 (MQ=255)

                               tGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTc                                                                                                     <  2:383154/140‑1 (MQ=255)

                                        tAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCaa                                                                                            >  2:9818/1‑140 (MQ=255)

                                               tAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCTCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGAGGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTc                                                                                     >  1:240234/1‑140 (MQ=255)

                                               tAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTc                                                                                     >  1:300423/1‑140 (MQ=255)

                                               tAAAGTGACCTCGGTAGAAGCAATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCGTTTCTTTTCGTGCTCGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACACACGTCCGTTCTCAATGGCTTc                                                                                     >  1:419626/1‑140 (MQ=255)

                                                aaaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTc                                                                                     >  1:1064751/1‑140 (MQ=255)

                                                aaaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCg                                                                                    >  2:1143469/1‑140 (MQ=255)

                                                      aCCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATg                                                                                                                                 <  1:215022/89‑1 (MQ=255)

                                                      aCCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATg                                                                                                                                 >  2:215022/1‑89 (MQ=255)

                                                       ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCCGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:255378/140‑1 (MQ=255)

                                                       ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  1:1143469/140‑1 (MQ=255)

                                                       ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:1004623/140‑1 (MQ=255)

                                                       ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:902028/140‑1 (MQ=255)

                                                       ccTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:563583/140‑1 (MQ=255)

                                                        cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:1111922/140‑1 (MQ=255)

                                                             tAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       <  1:26134/140‑1 (MQ=255)

                                                             tAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       <  1:415487/140‑1 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       >  1:990402/1‑140 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       >  1:495833/1‑140 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       >  1:690113/1‑140 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaa                                                                       >  1:378653/1‑140 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGAGGAGCGCGACAAACGTCCGTTCTCACTGGCTTCGACGCCGGATGaaa                                                                       >  1:369153/1‑140 (MQ=255)

                                                              aGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaaa                                                                      >  2:295678/1‑140 (MQ=255)

                                                                     aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCCGT‑TTTGAGGGTAGTGATGGATGAGCGCGACACACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGttt                                                               >  2:11016/1‑140 (MQ=255)

                                                                     aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGttt                                                               >  2:102140/1‑140 (MQ=255)

                                                                     aTCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGttt                                                               >  2:1023274/1‑140 (MQ=255)

                                                                      tCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTa                                                              <  2:135857/140‑1 (MQ=255)

                                                                      tCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGGGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTa                                                              <  2:1135538/140‑1 (MQ=255)

                                                                       cACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTAt                                                             >  2:806461/1‑140 (MQ=255)

                                                                           gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAg                                                         <  1:806461/140‑1 (MQ=255)

                                                                             tACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                       >  2:1084119/1‑140 (MQ=255)

                                                                             tACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                       <  2:127097/140‑1 (MQ=255)

                                                                                cGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCa                                                    >  2:3744/1‑140 (MQ=255)

                                                                                  tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             <  2:193275/113‑1 (MQ=255)

                                                                                  tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                             >  1:193275/1‑113 (MQ=255)

                                                                                  tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                  >  2:1026448/1‑140 (MQ=255)

                                                                                  tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                  >  2:1040604/1‑140 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTg                                               >  1:1087005/1‑140 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTg                                               >  1:1087755/1‑140 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTg                                               >  1:64185/1‑140 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTg                                               >  1:547188/1‑140 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               >  1:433365/1‑59 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGt                                                                                                                               <  2:433365/59‑1 (MQ=255)

                                                                                      tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTgg                                              <  1:3744/140‑1 (MQ=255)

                                                                                         tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATAGATGAGCGCGACAAACGTCCGTTCTCAATTGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgcgc                                           >  1:169182/1‑140 (MQ=255)

                                                                                          gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGAGGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCt                                          >  2:466216/1‑140 (MQ=255)

                                                                                            ccGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATTGCTTCTTCGCCGTATGAAAAATTGTTTATCGAGCTTCTTATTTgcttttg                                        <  1:9818/140‑6 (MQ=255)

                                                                                            ccGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                        >  1:1031376/1‑140 (MQ=255)

                                                                                                         gACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCt                            <  1:842757/140‑1 (MQ=255)

                                                                                                          aCGCGGCCTTTTCTTTTCGTGCTGGTCCGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCGTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAACAACCtt                           >  1:1052617/1‑140 (MQ=255)

                                                                                                          aCGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCtt                           >  1:991884/1‑140 (MQ=255)

                                                                                                              ggCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                      >  1:748939/1‑140 (MQ=255)

                                                                                                              ggCCTTTTCTTTTCGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                      >  1:406545/1‑140 (MQ=255)

                                                                                                                 cttttcttttCGTGCTGGTCAGT‑TTTGATGGTAGTGATAGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGaa                   <  2:169182/140‑1 (MQ=255)

                                                                                                                   tttcttttcGTGCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                 <  1:957776/140‑1 (MQ=255)

                                                                                                                          tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGc                <  2:568454/135‑1 (MQ=255)

                                                                                                                          tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGc                >  1:568454/1‑135 (MQ=255)

                                                                                                                          tcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa           >  1:582702/1‑140 (MQ=255)

                                                                                                                              gCTGGTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAc      <  2:1115697/140‑1 (MQ=255)

                                                                                                                                 ggTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGc   >  2:153643/1‑140 (MQ=255)

                                                                                                                                 ggTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGc   >  2:153531/1‑140 (MQ=255)

                                                                                                                                 ggTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGc   >  2:683755/1‑140 (MQ=255)

                                                                                                                                  gTCAGT‑TTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa  <  2:809578/140‑1 (MQ=255)

CCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCA  >  CP009273/4019857‑4020127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A