Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,020,166 C→A 100% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,020,1660CA97.1% 796.7 / 13.5 246D445Y (GAC→TAC) yedQputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (124/115);  total (128/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 247 total aligned reads displayed.

CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC  >  CP009273/2020019‑2020312
                                                                                                                                                   |                                                                                                                                                  
   ccTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTaa                                                                                                                                               <  1:149024/150‑1 (MQ=255)
   ccTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTaa                                                                                                                                               <  2:3088606/150‑1 (MQ=255)
   ccTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTaa                                                                                                                                               >  2:1612740/1‑150 (MQ=255)
     tCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATc                                                                                                                                             >  2:3607715/1‑150 (MQ=255)
       cGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGc                                                                                                                                           <  1:2896983/150‑1 (MQ=255)
       cGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGc                                                                                                                                           >  1:2600412/1‑150 (MQ=255)
        gTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCt                                                                                                                                          <  2:4076448/150‑1 (MQ=255)
         tCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCtt                                                                                                                                         <  2:754605/150‑1 (MQ=255)
          cAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCttt                                                                                                                                        <  2:2980026/150‑1 (MQ=255)
          cAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCttt                                                                                                                                        <  2:1623123/150‑1 (MQ=255)
             aCTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTaaa                                                                                                                                     <  2:980072/150‑1 (MQ=255)
               tCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAt                                                                                                                                   <  1:990394/150‑1 (MQ=255)
                  cgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGt                                                                                                                                <  1:3025001/150‑1 (MQ=255)
                                   caAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATg                                                                                                               <  1:3357107/150‑1 (MQ=255)
                                    aaaaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCATCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGa                                                                                                              <  2:1207212/150‑1 (MQ=255)
                                    aaaaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGa                                                                                                              >  2:131596/1‑150 (MQ=255)
                                      aaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACa                                                                                                            >  2:1212159/1‑150 (MQ=255)
                                      aaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACa                                                                                                            <  2:3821879/150‑1 (MQ=255)
                                            tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                      >  1:857643/1‑150 (MQ=255)
                                            tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                      >  2:2877192/1‑150 (MQ=255)
                                             caccacCGACCCGCACGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAg                                                                                                     >  1:3808593/1‑150 (MQ=255)
                                                   cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGtt                                                                                               <  1:3509744/150‑1 (MQ=255)
                                                    gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                              >  1:2797241/1‑150 (MQ=255)
                                                     aCCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAGGATCGACCTGAATGACAGAAAAGGGGTGTggg                                                                                             >  1:4400302/1‑150 (MQ=255)
                                                     aCCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTgg                                                                                             >  1:343648/1‑150 (MQ=255)
                                                     aCCCGCCCGGCAACGTCCTGCGCACGCAAGGAACGGCTAATTTATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAACGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTgg                                                                                             <  2:822222/150‑1 (MQ=255)
                                                      cccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGt                                                                                            >  1:1571643/1‑150 (MQ=255)
                                                       ccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTg                                                                                           >  2:2377905/1‑150 (MQ=255)
                                                       ccgcccgGCAACGTCCAGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCTCCTGATGACCAAAGCGGTAATTAATCGCTGTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTg                                                                                           >  1:1824009/1‑150 (MQ=255)
                                                         gcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCg                                                                                         <  1:518685/150‑1 (MQ=255)
                                                             ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                     >  2:114814/1‑150 (MQ=255)
                                                             ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                     <  1:3607715/150‑1 (MQ=255)
                                                             ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                     >  2:1685560/1‑150 (MQ=255)
                                                                aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                                  <  1:4079768/150‑1 (MQ=255)
                                                                aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                                  >  1:3212113/1‑150 (MQ=255)
                                                                aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                                  >  2:2489138/1‑150 (MQ=255)
                                                                 aCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGacac                                                                                 >  2:2063221/1‑150 (MQ=255)
                                                                  cGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGAcaca                                                                                >  2:1004694/1‑150 (MQ=255)
                                                                    tCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAt                                                                              <  1:2080965/150‑1 (MQ=255)
                                                                     ccTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAtt                                                                             <  1:4467599/150‑1 (MQ=255)
                                                                        gcgcACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTag                                                                          <  1:3202910/150‑1 (MQ=255)
                                                                        gcgcACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAGTTag                                                                          >  1:472664/1‑150 (MQ=255)
                                                                           cacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcga                                                                       >  1:219127/1‑150 (MQ=255)
                                                                             cgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgagc                                                                     <  2:2234467/150‑1 (MQ=255)
                                                                              gcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgagcg                                                                    >  1:2660057/1‑150 (MQ=255)
                                                                               caAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCgg                                                                   >  2:3998212/1‑150 (MQ=255)
                                                                                taGGAACTGCTAATTAATCCGGCTGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAATTGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGa                                                                  <  2:4552391/149‑1 (MQ=255)
                                                                                aaGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGa                                                                  <  1:2519789/150‑1 (MQ=255)
                                                                                aaGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGa                                                                  <  1:2706993/150‑1 (MQ=255)
                                                                                 aGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAc                                                                 >  1:2596747/1‑150 (MQ=255)
                                                                                  ggAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACg                                                                >  1:1042857/1‑150 (MQ=255)
                                                                                   gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                               >  1:2436371/1‑150 (MQ=255)
                                                                                   gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                               <  1:2854031/150‑1 (MQ=255)
                                                                                   gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                               >  2:3205880/1‑150 (MQ=255)
                                                                                    aaCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                              >  1:4161151/1‑150 (MQ=255)
                                                                                    aaCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                              >  1:4161174/1‑150 (MQ=255)
                                                                                      cTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCt                                                            >  1:2312187/1‑150 (MQ=255)
                                                                                        gCTAATGAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAACGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttt                                                          <  2:202862/150‑1 (MQ=255)
                                                                                         cTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtttt                                                         >  2:667812/1‑150 (MQ=255)
                                                                                         cTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtttt                                                         >  2:1399139/1‑150 (MQ=255)
                                                                                            attaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCg                                                      <  1:2979881/150‑1 (MQ=255)
                                                                                              taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGaa                                                    >  1:4072137/1‑150 (MQ=255)
                                                                                              taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGaa                                                    >  2:4279117/1‑150 (MQ=255)
                                                                                               aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                   >  1:3970220/1‑150 (MQ=255)
                                                                                                atCCGGCAGCATGATAAAGAACACGGTCGCCCGCCTGATGACCAGAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACa                                                  <  1:2089855/150‑1 (MQ=255)
                                                                                                   cGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                               >  1:4492086/1‑150 (MQ=255)
                                                                                                   cGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                               <  2:2666352/150‑1 (MQ=255)
                                                                                                    ggCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgc                                              <  2:3359133/150‑1 (MQ=255)
                                                                                                    ggCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgc                                              <  2:2332495/150‑1 (MQ=255)
                                                                                                    ggCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgc                                              >  1:511251/1‑150 (MQ=255)
                                                                                                      cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcgc                                            >  2:2539816/1‑150 (MQ=255)
                                                                                                        gcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                          <  2:4145958/150‑1 (MQ=255)
                                                                                                        gcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                          <  2:896049/150‑1 (MQ=255)
                                                                                                        gcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                          <  2:516763/150‑1 (MQ=255)
                                                                                                         caTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAc                                         >  1:926563/1‑150 (MQ=255)
                                                                                                          aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                        >  1:162015/1‑150 (MQ=255)
                                                                                                          aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                        >  1:442154/1‑150 (MQ=255)
                                                                                                           tGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACgg                                       >  2:2580907/1‑150 (MQ=255)
                                                                                                             agaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGtt                                     >  1:613072/1‑150 (MQ=255)
                                                                                                              gaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTta                                    >  2:4339016/1‑150 (MQ=255)
                                                                                                                aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCTAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                  <  2:600458/150‑1 (MQ=255)
                                                                                                                aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                  >  1:941904/1‑150 (MQ=255)
                                                                                                                  gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatata                                <  1:1627011/150‑1 (MQ=255)
                                                                                                                  gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatata                                >  1:3718424/1‑150 (MQ=255)
                                                                                                                    acacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaaa                              >  1:2047307/1‑150 (MQ=255)
                                                                                                                    acacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaaa                              >  1:631265/1‑150 (MQ=255)
                                                                                                                          tCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGtt                        <  2:1571643/150‑1 (MQ=255)
                                                                                                                           cgcccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTa                       <  2:4032691/150‑1 (MQ=255)
                                                                                                                            gcccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTaa                      >  2:929083/1‑150 (MQ=255)
                                                                                                                             cccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAg                     >  2:1803837/1‑150 (MQ=255)
                                                                                                                              ccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAgg                    >  1:966799/1‑150 (MQ=255)
                                                                                                                              ccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAgg                    <  1:2029377/150‑1 (MQ=255)
                                                                                                                               cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgt                   <  1:1722055/150‑1 (MQ=255)
                                                                                                                               cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgt                   <  2:4416466/150‑1 (MQ=255)
                                                                                                                               cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGGTATATAAACGCGTTAAGgt                   >  2:1981718/1‑150 (MQ=255)
                                                                                                                                    gatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt              <  1:454123/150‑1 (MQ=255)
                                                                                                                                        aCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCa          <  2:2749238/150‑1 (MQ=255)
                                                                                                                                        aCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCa          <  2:2456141/150‑1 (MQ=255)
                                                                                                                                              gCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTg    <  2:3333575/150‑1 (MQ=255)
                                                                                                                                                ggTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGcc  <  2:4571773/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC  >  CP009273/2020019‑2020312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973072.2461478/1‑150 (MQ=60)
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACACCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGTCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973072.1259820/1‑150 (MQ=60)
  CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAT                                                                                                                                                     <  SRR19973072.2950102/150‑1 (MQ=60)
  CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAT                                                                                                                                                     <  SRR19973072.2950104/150‑1 (MQ=60)
   AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    >  SRR19973072.1485957/1‑150 (MQ=60)
    GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973072.242985/150‑1 (MQ=60)
     CCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAA                                                                                                                                                  <  SRR19973072.149403/150‑1 (MQ=60)
       TCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTAATGACCAAAGCGGTAATTAATC                                                                                                                                                <  SRR19973072.15163/150‑1 (MQ=60)
         CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              <  SRR19973072.2903187/150‑1 (MQ=60)
         CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              >  SRR19973072.2606011/1‑150 (MQ=60)
         CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              >  SRR19973072.2605971/1‑150 (MQ=60)
          GTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCT                                                                                                                                             <  SRR19973072.4095419/150‑1 (MQ=60)
            CAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTT                                                                                                                                           >  SRR19973072.3628690/1‑150 (MQ=60)
            CAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTT                                                                                                                                           <  SRR19973072.1706447/150‑1 (MQ=60)
              GACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAA                                                                                                                                         >  SRR19973072.4518574/1‑150 (MQ=60)
                 TCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAT                                                                                                                                      <  SRR19973072.992560/150‑1 (MQ=60)
                  CGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATG                                                                                                                                     <  SRR19973072.4478763/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973072.3031332/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973072.3031413/150‑1 (MQ=60)
                     GCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTC                                                                                                                                  <  SRR19973072.4092239/150‑1 (MQ=60)
                      CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCA                                                                                                                                 >  SRR19973072.522250/1‑150 (MQ=60)
                            AGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCG                                                                                                                           >  SRR19973072.944905/1‑150 (MQ=60)
                            AGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCG                                                                                                                           >  SRR19973072.4582240/1‑150 (MQ=60)
                              ACTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAC                                                                                                                         <  SRR19973072.488636/150‑1 (MQ=60)
                                TCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCT                                                                                                                       <  SRR19973072.3895491/150‑1 (MQ=60)
                                     CAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATG                                                                                                                  <  SRR19973072.3364282/150‑1 (MQ=60)
                                     CAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATG                                                                                                                  <  SRR19973072.3364227/150‑1 (MQ=60)
                                      AAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGA                                                                                                                 <  SRR19973072.2614849/150‑1 (MQ=60)
                                          CTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGA                                                                                                             <  SRR19973072.2085565/150‑1 (MQ=60)
                                              TCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAA                                                                                                         >  SRR19973072.859490/1‑150 (MQ=60)
                                               CACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAG                                                                                                        >  SRR19973072.482044/1‑150 (MQ=60)
                                               CACCACCGACCCGCACGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAG                                                                                                        >  SRR19973072.3816567/1‑150 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    <  SRR19973072.3633621/150‑1 (MQ=60)
                                                    CCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGT                                                                                                   <  SRR19973072.4386186/150‑1 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973072.3517107/150‑1 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973072.3517062/150‑1 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973072.3808770/1‑150 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973072.2803225/1‑150 (MQ=60)
                                                       ACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAGGATCGACCTGAATGACAGAAAAggggtgtggg                                                                                                >  SRR19973072.4409557/1‑140 (MQ=60)
                                                       ACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGG                                                                                                >  SRR19973072.344417/1‑150 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               <  SRR19973072.3542713/150‑1 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               >  SRR19973072.1574999/1‑150 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               <  SRR19973072.733940/150‑1 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               >  SRR19973072.257949/1‑150 (MQ=60)
                                                         CCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTG                                                                                              >  SRR19973072.1828073/1‑150 (MQ=60)
                                                         CCGCCCGGCAACGTCCAGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCTCCTGATGACCAAAGCGGTAATTAATCGCTGTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTG                                                                                              >  SRR19973072.1827982/1‑150 (MQ=60)
                                                           GCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCG                                                                                            <  SRR19973072.519922/150‑1 (MQ=60)
                                                            CCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGT                                                                                           >  SRR19973072.2449624/1‑150 (MQ=60)
                                                              CGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCT                                                                                         <  SRR19973072.2383752/150‑1 (MQ=60)
                                                               GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTG                                                                                        <  SRR19973072.3615318/150‑1 (MQ=60)
                                                                 CAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGAC                                                                                      >  SRR19973072.2044500/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     <  SRR19973072.4088320/150‑1 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     <  SRR19973072.4088331/150‑1 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973072.3495445/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973072.3218906/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973072.3218824/1‑150 (MQ=60)
                                                                    CGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACA                                                                                   <  SRR19973072.785815/150‑1 (MQ=60)
                                                                    CGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACA                                                                                   <  SRR19973072.4410780/150‑1 (MQ=60)
                                                                      TCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAT                                                                                 <  SRR19973072.2085501/150‑1 (MQ=60)
                                                                       CCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATT                                                                                <  SRR19973072.4477024/150‑1 (MQ=60)
                                                                        CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTT                                                                               <  SRR19973072.325957/150‑1 (MQ=60)
                                                                          GCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAG                                                                             <  SRR19973072.3209679/150‑1 (MQ=60)
                                                                          GCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAG                                                                             >  SRR19973072.1589935/1‑150 (MQ=60)
                                                                          GCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAGTTAG                                                                             >  SRR19973072.473773/1‑150 (MQ=60)
                                                                           CGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGC                                                                            <  SRR19973072.3995746/150‑1 (MQ=60)
                                                                            GCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCG                                                                           <  SRR19973072.1779144/150‑1 (MQ=60)
                                                                             CACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGA                                                                          >  SRR19973072.219678/1‑150 (MQ=60)
                                                                               CGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGC                                                                        <  SRR19973072.2061914/150‑1 (MQ=60)
                                                                               CGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGC                                                                        <  SRR19973072.2061899/150‑1 (MQ=60)
                                                                                GCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCG                                                                       >  SRR19973072.2665753/1‑150 (MQ=60)
                                                                                GCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCG                                                                       <  SRR19973072.216831/150‑1 (MQ=60)
                                                                                 CAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGG                                                                      <  SRR19973072.2710763/150‑1 (MQ=60)
                                                                                 CAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGG                                                                      >  SRR19973072.4390607/1‑150 (MQ=60)
                                                                                  AAGGAACTGCTAATTACTCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     <  SRR19973072.2712764/150‑1 (MQ=60)
                                                                                  AAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     <  SRR19973072.2525264/150‑1 (MQ=60)
                                                                                  AAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     <  SRR19973072.2712624/150‑1 (MQ=60)
                                                                                  AAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     <  SRR19973072.2712760/150‑1 (MQ=60)
                                                                                   AGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAC                                                                    >  SRR19973072.2602344/1‑150 (MQ=60)
                                                                                    GGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACG                                                                   >  SRR19973072.1045106/1‑150 (MQ=60)
                                                                                    GGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACG                                                                   >  SRR19973072.303526/1‑150 (MQ=60)
                                                                                     GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  >  SRR19973072.2441651/1‑150 (MQ=60)
                                                                                     GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  <  SRR19973072.2860376/150‑1 (MQ=60)
                                                                                     GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  <  SRR19973072.2860142/150‑1 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 >  SRR19973072.4169895/1‑150 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 >  SRR19973072.4169888/1‑150 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 >  SRR19973072.4169872/1‑150 (MQ=60)
                                                                                        CTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCT                                                               >  SRR19973072.2317120/1‑150 (MQ=60)
                                                                                        CTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCT                                                               >  SRR19973072.2316387/1‑150 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973072.866931/150‑1 (MQ=60)
                                                                                            TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           <  SRR19973072.1702506/150‑1 (MQ=60)
                                                                                              ATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCG                                                         <  SRR19973072.2986236/150‑1 (MQ=60)
                                                                                                TAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAA                                                       >  SRR19973072.4080681/1‑150 (MQ=60)
                                                                                                 AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973072.3978527/1‑150 (MQ=60)
                                                                                                  ATCCGGCAGCATGATAAAGAACACGGTCGCCCGCCTGATGACCAGAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     <  SRR19973072.2094410/150‑1 (MQ=60)
                                                                                                    CCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGT                                                   <  SRR19973072.2595666/150‑1 (MQ=60)
                                                                                                    CCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGT                                                   <  SRR19973072.1593599/150‑1 (MQ=60)
                                                                                                     CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  >  SRR19973072.4501711/1‑150 (MQ=60)
                                                                                                     CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  >  SRR19973072.4501576/1‑150 (MQ=60)
                                                                                                      GGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGC                                                 >  SRR19973072.512480/1‑150 (MQ=60)
                                                                                                      GGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGC                                                 >  SRR19973072.512470/1‑150 (MQ=60)
                                                                                                           CATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAC                                            >  SRR19973072.928576/1‑150 (MQ=60)
                                                                                                           CATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAC                                            >  SRR19973072.928721/1‑150 (MQ=60)
                                                                                                            ATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACG                                           >  SRR19973072.162426/1‑150 (MQ=60)
                                                                                                            ATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACG                                           >  SRR19973072.443177/1‑150 (MQ=60)
                                                                                                               AGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTT                                        >  SRR19973072.614479/1‑150 (MQ=60)
                                                                                                                  AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     >  SRR19973072.943963/1‑150 (MQ=60)
                                                                                                                   AGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATAT                                    <  SRR19973072.3853784/150‑1 (MQ=60)
                                                                                                                    GAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATA                                   <  SRR19973072.1630548/150‑1 (MQ=60)
                                                                                                                    GAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATA                                   >  SRR19973072.3726253/1‑150 (MQ=60)
                                                                                                                     AACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAA                                  <  SRR19973072.543578/150‑1 (MQ=60)
                                                                                                                      ACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAA                                 >  SRR19973072.632395/1‑150 (MQ=60)
                                                                                                                      ACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAA                                 >  SRR19973072.632706/1‑150 (MQ=60)
                                                                                                                      ACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAA                                 >  SRR19973072.2051758/1‑150 (MQ=60)
                                                                                                                                CCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGG                       <  SRR19973072.2033771/150‑1 (MQ=60)
                                                                                                                                CCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGG                       >  SRR19973072.968912/1‑150 (MQ=60)
                                                                                                                                CCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGG                       >  SRR19973072.968940/1‑150 (MQ=60)
                                                                                                                                 CGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGT                      <  SRR19973072.1725827/150‑1 (MQ=60)
                                                                                                                                 CGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGT                      <  SRR19973072.3068012/150‑1 (MQ=60)
                                                                                                                                  GCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTG                     <  SRR19973072.1624771/150‑1 (MQ=60)
                                                                                                                                     TGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCG                  >  SRR19973072.3319813/1‑150 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 <  SRR19973072.455175/150‑1 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 <  SRR19973072.1900386/150‑1 (MQ=60)
                                                                                                                                       ATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTG                >  SRR19973072.283125/1‑150 (MQ=60)
                                                                                                                                       ATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTG                >  SRR19973072.283805/1‑150 (MQ=60)
                                                                                                                                          ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973072.2132808/1‑150 (MQ=60)
                                                                                                                                              AAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCC         >  SRR19973072.4463494/1‑150 (MQ=60)
                                                                                                                                              AAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCC         >  SRR19973072.4462860/1‑150 (MQ=60)
                                                                                                                                                  GGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC     <  SRR19973072.934373/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  <  SRR19973072.1259716/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  SRR19973072.1155832/1‑150 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  SRR19973072.1155523/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑