Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,020,166 C→A 93.3% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,020,1660CA93.3% 822.4 / 45.3 272D445Y (GAC→TAC) yedQputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (7/12);  new base A (109/144);  total (116/156)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 274 total aligned reads displayed.

AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT  >  CP009273/2020020‑2020309
                                                                                                                                                  |                                                                                                                                               
aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                              >  2:3476730/1‑150 (MQ=255)
aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                              <  1:330458/150‑1 (MQ=255)
  ccTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGACCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTaa                                                                                                                                            >  2:4116555/1‑150 (MQ=255)
   cTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGtaattaat                                                                                                                                           >  1:1107123/1‑150 (MQ=255)
       gTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCt                                                                                                                                       >  1:2556587/1‑150 (MQ=255)
          aGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTa                                                                                                                                    <  2:797696/150‑1 (MQ=255)
             cTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTaaaa                                                                                                                                 <  2:305628/150‑1 (MQ=255)
                gcgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATgg                                                                                                                              >  2:3905445/1‑150 (MQ=255)
                 cgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGt                                                                                                                             <  1:281603/150‑1 (MQ=255)
                  gcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGACAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTc                                                                                                                            >  2:1133392/1‑150 (MQ=255)
                   ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCa                                                                                                                           >  1:2368944/1‑150 (MQ=255)
                    cTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCaa                                                                                                                          >  1:2491427/1‑150 (MQ=255)
                      ggCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGa                                                                                                                        <  1:1463686/150‑1 (MQ=255)
                       gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                       <  2:4232306/150‑1 (MQ=255)
                       gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                       <  1:2797024/150‑1 (MQ=255)
                        cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                      <  2:1510230/150‑1 (MQ=255)
                        cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                      >  1:3934574/1‑150 (MQ=255)
                        cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                      <  2:5023124/150‑1 (MQ=255)
                        cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                      <  2:1177055/150‑1 (MQ=255)
                                cacaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGaa                                                                                                              <  1:1178599/150‑1 (MQ=255)
                                cacaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGaa                                                                                                              <  1:4750376/150‑1 (MQ=255)
                                         cctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaa                                                                                                     >  2:1590930/1‑150 (MQ=255)
                                         cctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaa                                                                                                     >  2:4072149/1‑150 (MQ=255)
                                          ctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaa                                                                                                    >  1:4622750/1‑150 (MQ=255)
                                           tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                   >  1:2447545/1‑150 (MQ=255)
                                           tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                   <  2:4634438/150‑1 (MQ=255)
                                           tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                   <  2:2079375/150‑1 (MQ=255)
                                           tcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaaa                                                                                                   >  1:1233223/1‑150 (MQ=255)
                                               caccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAt                                                                                               <  1:2181178/150‑1 (MQ=255)
                                               caccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAt                                                                                               <  1:2181096/150‑1 (MQ=255)
                                                accGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATg                                                                                              >  1:1588698/1‑150 (MQ=255)
                                                accGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATg                                                                                              >  1:4261034/1‑150 (MQ=255)
                                                accGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATg                                                                                              <  1:1001307/150‑1 (MQ=255)
                                                   gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGTCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                           >  2:540890/1‑150 (MQ=255)
                                                     cccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGt                                                                                         <  1:659975/150‑1 (MQ=255)
                                                      ccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTg                                                                                        <  1:601667/150‑1 (MQ=255)
                                                       cgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGc                                                                                       <  1:2911367/150‑1 (MQ=255)
                                                        gcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGATTGACAGAAAAAGGATGTTGGTGCg                                                                                      <  2:3381150/150‑1 (MQ=255)
                                                         cccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGt                                                                                     >  1:1706777/1‑150 (MQ=255)
                                                             gCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGa                                                                                 >  2:4176071/1‑150 (MQ=255)
                                                              cAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGac                                                                                <  2:4362062/150‑1 (MQ=255)
                                                              cAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGac                                                                                >  2:1101868/1‑150 (MQ=255)
                                                              cAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGac                                                                                >  2:1087565/1‑150 (MQ=255)
                                                               aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                               <  1:1598467/150‑1 (MQ=255)
                                                               aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                               >  1:3110816/1‑150 (MQ=255)
                                                                 cGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGAcaca                                                                             >  1:4548441/1‑150 (MQ=255)
                                                                   tCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAt                                                                           <  1:4898677/150‑1 (MQ=255)
                                                                     cTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAttt                                                                         <  2:1503990/150‑1 (MQ=255)
                                                                         gcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcg                                                                     <  2:500138/150‑1 (MQ=255)
                                                                                aGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAc                                                              <  1:3853712/150‑1 (MQ=255)
                                                                                aGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAc                                                              <  2:4864565/150‑1 (MQ=255)
                                                                                 ggAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACg                                                             >  1:4463086/1‑150 (MQ=255)
                                                                                  gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                            >  1:787326/1‑150 (MQ=255)
                                                                                   aaCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                           <  1:209328/150‑1 (MQ=255)
                                                                                      tGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtt                                                        <  1:1871904/150‑1 (MQ=255)
                                                                                         taattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttttt                                                     <  1:4427948/150‑1 (MQ=255)
                                                                                         taattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttttt                                                     <  2:4437695/150‑1 (MQ=255)
                                                                                          aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCACAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                    <  2:2429337/150‑1 (MQ=255)
                                                                                          aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                    <  2:5113626/150‑1 (MQ=255)
                                                                                          aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                    <  1:5065562/150‑1 (MQ=255)
                                                                                          aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                    <  2:2192211/150‑1 (MQ=255)
                                                                                            ttaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGa                                                  <  2:49390/150‑1 (MQ=255)
                                                                                            ttaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGa                                                  <  2:2253404/150‑1 (MQ=255)
                                                                                                 ccGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGt                                             <  1:981312/150‑1 (MQ=255)
                                                                                                 ccGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGt                                             >  2:3475242/1‑150 (MQ=255)
                                                                                                    gcagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcg                                          <  1:3744396/150‑1 (MQ=255)
                                                                                                     cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcgc                                         <  2:140337/150‑1 (MQ=255)
                                                                                                      agcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                        >  2:1702748/1‑150 (MQ=255)
                                                                                                         aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                     >  2:2655/1‑150 (MQ=255)
                                                                                                          tGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACgg                                    <  1:4100359/150‑1 (MQ=255)
                                                                                                           gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                   >  2:4314236/1‑150 (MQ=255)
                                                                                                           gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                   <  1:3591491/150‑1 (MQ=255)
                                                                                                              aaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtat                                <  2:1094231/150‑1 (MQ=255)
                                                                                                              aaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtat                                >  1:1315820/1‑150 (MQ=255)
                                                                                                                agaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatat                              >  2:759752/1‑150 (MQ=255)
                                                                                                                    cacGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAc                          <  2:4456045/150‑1 (MQ=255)
                                                                                                                     acGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcg                         >  2:1433853/1‑150 (MQ=255)
                                                                                                                     acGGTCGCCCGCCTGATGACCAAAGCGGGAAGTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGCACAGTGCGCCACGGGtctatccacg                         >  2:1281312/1‑145 (MQ=255)
                                                                                                                       ggTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgcg                       >  2:3192483/1‑150 (MQ=255)
                                                                                                                           gcccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTaa                   <  1:4151113/150‑1 (MQ=255)
                                                                                                                             ccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTCTATAAACGCGTGAAgg                 >  2:297109/1‑150 (MQ=255)
                                                                                                                              cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgt                <  1:3190998/150‑1 (MQ=255)
                                                                                                                               gccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtg               >  2:392788/1‑150 (MQ=255)
                                                                                                                                ccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtgt              <  2:54870/150‑1 (MQ=255)
                                                                                                                                 cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGACAAAGGAGGTTGGTGCGTCTGACACACTTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTGCTATCAACGCGTGAAGGAGTc             >  2:3406061/1‑150 (MQ=255)
                                                                                                                                 cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc             >  1:4359709/1‑150 (MQ=255)
                                                                                                                                 cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc             >  2:3268919/1‑150 (MQ=255)
                                                                                                                                   gatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt           >  2:2811373/1‑150 (MQ=255)
                                                                                                                                   gatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt           <  2:1930636/150‑1 (MQ=255)
                                                                                                                                   gatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt           <  2:344502/150‑1 (MQ=255)
                                                                                                                                    atgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTg          <  2:3222975/150‑1 (MQ=255)
                                                                                                                                     tgaCCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGc         <  2:1059758/150‑1 (MQ=255)
                                                                                                                                      gaCCAACGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc        <  1:3114786/150‑1 (MQ=255)
                                                                                                                                      gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc        <  1:2268892/150‑1 (MQ=255)
                                                                                                                                      gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc        <  2:3168582/150‑1 (MQ=255)
                                                                                                                                       aCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCa       >  1:3025643/1‑150 (MQ=255)
                                                                                                                                        ccAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCAc      <  2:257662/150‑1 (MQ=255)
                                                                                                                                        ccAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCAc      <  1:4824244/150‑1 (MQ=255)
                                                                                                                                         cAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACg     >  2:3793159/1‑150 (MQ=255)
                                                                                                                                            aGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCt  <  2:4752492/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT  >  CP009273/2020020‑2020309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       <  SRR19973077.3681672/150‑1 (MQ=60)
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973077.1215140/1‑150 (MQ=60)
   AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973077.4425984/150‑1 (MQ=60)
   AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973077.330921/150‑1 (MQ=60)
   AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973077.331039/150‑1 (MQ=60)
    GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973077.2297867/150‑1 (MQ=60)
    GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973077.2954519/150‑1 (MQ=60)
      CTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAAT                                                                                                                                                 >  SRR19973077.1109056/1‑150 (MQ=60)
       TCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATC                                                                                                                                                <  SRR19973077.2872448/150‑1 (MQ=60)
          GTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCT                                                                                                                                             >  SRR19973077.2561115/1‑150 (MQ=60)
           TCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTT                                                                                                                                            <  SRR19973077.4778697/150‑1 (MQ=60)
            CAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTT                                                                                                                                           >  SRR19973077.5055673/1‑150 (MQ=60)
               ACTCGCGCCGGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAACGCGGTAATTAATCGCTTTAAA                                                                                                                                        <  SRR19973077.2385845/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973077.4407758/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973077.282112/150‑1 (MQ=60)
                      CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCA                                                                                                                                 >  SRR19973077.2373128/1‑150 (MQ=60)
                       CTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAA                                                                                                                                >  SRR19973077.2495834/1‑150 (MQ=60)
                         GGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGA                                                                                                                              <  SRR19973077.1466228/150‑1 (MQ=60)
                          GCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAT                                                                                                                             <  SRR19973077.2802025/150‑1 (MQ=60)
                          GCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAT                                                                                                                             <  SRR19973077.2801999/150‑1 (MQ=60)
                           CAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATC                                                                                                                            >  SRR19973077.3941519/1‑150 (MQ=60)
                              AATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAC                                                                                                                         >  SRR19973077.1962688/1‑150 (MQ=60)
                               ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        <  SRR19973077.1344748/150‑1 (MQ=60)
                                   CACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAA                                                                                                                    <  SRR19973077.1180809/150‑1 (MQ=60)
                                   CACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAA                                                                                                                    <  SRR19973077.1180650/150‑1 (MQ=60)
                                   CACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAA                                                                                                                    <  SRR19973077.4758764/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973077.5096578/150‑1 (MQ=60)
                                     CAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATG                                                                                                                  <  SRR19973077.2296394/150‑1 (MQ=60)
                                         ACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAG                                                                                                              <  SRR19973077.4185190/150‑1 (MQ=60)
                                         ACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAG                                                                                                              <  SRR19973077.3556363/150‑1 (MQ=60)
                                          CTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGA                                                                                                             <  SRR19973077.1357639/150‑1 (MQ=60)
                                            CCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAA                                                                                                           <  SRR19973077.2141046/150‑1 (MQ=60)
                                             CTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAA                                                                                                          >  SRR19973077.4630893/1‑150 (MQ=60)
                                              TCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAA                                                                                                         >  SRR19973077.1235365/1‑150 (MQ=60)
                                              TCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAA                                                                                                         >  SRR19973077.3812021/1‑150 (MQ=60)
                                              TCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAA                                                                                                         >  SRR19973077.2451878/1‑150 (MQ=60)
                                                  CACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAT                                                                                                     <  SRR19973077.2184870/150‑1 (MQ=60)
                                                  CACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAT                                                                                                     <  SRR19973077.2184952/150‑1 (MQ=60)
                                                  CACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGCCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGCAT                                                                                                     <  SRR19973077.2184951/150‑1 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    <  SRR19973077.1003076/150‑1 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    >  SRR19973077.4268514/1‑150 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    >  SRR19973077.1591462/1‑150 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    >  SRR19973077.1591490/1‑150 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  >  SRR19973077.2733114/1‑150 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973077.2267228/1‑150 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973077.388664/1‑150 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973077.2266972/1‑150 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               <  SRR19973077.3055122/150‑1 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               <  SRR19973077.661155/150‑1 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               <  SRR19973077.661094/150‑1 (MQ=60)
                                                         CCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTG                                                                                              <  SRR19973077.4837820/150‑1 (MQ=60)
                                                         CCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTG                                                                                              <  SRR19973077.602742/150‑1 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             <  SRR19973077.1506788/150‑1 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             >  SRR19973077.1621187/1‑150 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             >  SRR19973077.4291124/1‑150 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             <  SRR19973077.2916520/150‑1 (MQ=60)
                                                            CCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGT                                                                                           >  SRR19973077.1709722/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     <  SRR19973077.1601247/150‑1 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.805279/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.805329/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.3116376/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.3116327/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.3116356/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973077.451414/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     <  SRR19973077.4569399/150‑1 (MQ=60)
                                                                    CGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACA                                                                                   >  SRR19973077.4556471/1‑150 (MQ=60)
                                                                    CGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACA                                                                                   >  SRR19973077.483695/1‑150 (MQ=60)
                                                                      TCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAT                                                                                 <  SRR19973077.4907382/150‑1 (MQ=60)
                                                                       CCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATT                                                                                >  SRR19973077.116780/1‑150 (MQ=60)
                                                                         TGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTA                                                                              <  SRR19973077.4927754/150‑1 (MQ=60)
                                                                           CGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGC                                                                            >  SRR19973077.2122676/1‑150 (MQ=60)
                                                                            GCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCG                                                                           >  SRR19973077.2621434/1‑150 (MQ=60)
                                                                              ACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAG                                                                         <  SRR19973077.684358/150‑1 (MQ=60)
                                                                                   AGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAC                                                                    <  SRR19973077.3860482/150‑1 (MQ=60)
                                                                                    GGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACG                                                                   >  SRR19973077.4470991/1‑150 (MQ=60)
                                                                                    GGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACG                                                                   >  SRR19973077.4471142/1‑150 (MQ=60)
                                                                                    GGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTGGGTGCGTCTGACACAATTTAGCGAGCGGCCG                                                                   >  SRR19973077.4655267/1‑150 (MQ=60)
                                                                                     GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  >  SRR19973077.788697/1‑150 (MQ=60)
                                                                                     GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  <  SRR19973077.3191410/150‑1 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 <  SRR19973077.1150887/150‑1 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 <  SRR19973077.209720/150‑1 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973077.1071705/150‑1 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973077.1875184/150‑1 (MQ=60)
                                                                                           CTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTT                                                            >  SRR19973077.3290388/1‑150 (MQ=60)
                                                                                            TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           <  SRR19973077.254953/150‑1 (MQ=60)
                                                                                            TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           <  SRR19973077.255033/150‑1 (MQ=60)
                                                                                            TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           <  SRR19973077.4435791/150‑1 (MQ=60)
                                                                                             AATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          <  SRR19973077.5074544/150‑1 (MQ=60)
                                                                                              ATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCG                                                         <  SRR19973077.2491929/150‑1 (MQ=60)
                                                                                                  ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     <  SRR19973077.4447261/150‑1 (MQ=60)
                                                                                                    CCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGT                                                   <  SRR19973077.983044/150‑1 (MQ=60)
                                                                                                     CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  <  SRR19973077.1850623/150‑1 (MQ=60)
                                                                                                       GCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCG                                                <  SRR19973077.3870762/150‑1 (MQ=60)
                                                                                                       GCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCG                                                <  SRR19973077.3750984/150‑1 (MQ=60)
                                                                                                         AGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCC                                              >  SRR19973077.4619311/1‑150 (MQ=60)
                                                                                                             TGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGG                                          <  SRR19973077.4107594/150‑1 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         <  SRR19973077.3597848/150‑1 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         <  SRR19973077.281254/150‑1 (MQ=60)
                                                                                                               AGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTT                                        <  SRR19973077.1194033/150‑1 (MQ=60)
                                                                                                               AGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTT                                        >  SRR19973077.3824705/1‑150 (MQ=60)
                                                                                                                 AAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTAT                                      >  SRR19973077.1318129/1‑150 (MQ=60)
                                                                                                                  AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     <  SRR19973077.399017/150‑1 (MQ=60)
                                                                                                                    GAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATA                                   >  SRR19973077.411027/1‑150 (MQ=60)
                                                                                                                     AACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAA                                  <  SRR19973077.3736003/150‑1 (MQ=60)
                                                                                                                      ACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAA                                 >  SRR19973077.2436969/1‑150 (MQ=60)
                                                                                                                          GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             >  SRR19973077.5106441/1‑150 (MQ=60)
                                                                                                                             CGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTA                          >  SRR19973077.1865819/1‑150 (MQ=60)
                                                                                                                              GCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAA                         <  SRR19973077.4158423/150‑1 (MQ=60)
                                                                                                                                 CGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGT                      <  SRR19973077.3196655/150‑1 (MQ=60)
                                                                                                                                 CGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGT                      <  SRR19973077.3028722/150‑1 (MQ=60)
                                                                                                                                  GCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTG                     >  SRR19973077.4723658/1‑150 (MQ=60)
                                                                                                                                   CCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGT                    <  SRR19973077.3487326/150‑1 (MQ=60)
                                                                                                                                    CTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTC                   >  SRR19973077.4367409/1‑150 (MQ=60)
                                                                                                                                     TGATGACCAAAGCGGTCATTAATCTCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATTAACGCGTTAAGGTGTCG                  <  SRR19973077.1593697/150‑1 (MQ=60)
                                                                                                                                     TGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCG                  <  SRR19973077.4295962/150‑1 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 >  SRR19973077.4098573/1‑150 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 >  SRR19973077.3033001/1‑150 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 >  SRR19973077.1533327/1‑150 (MQ=60)
                                                                                                                                         GACCAACGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCC              <  SRR19973077.3120306/150‑1 (MQ=60)
                                                                                                                                         GACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCC              <  SRR19973077.2272885/150‑1 (MQ=60)
                                                                                                                                          ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973077.3030969/1‑150 (MQ=60)
                                                                                                                                          ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973077.1262504/1‑150 (MQ=60)
                                                                                                                                           CCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCAC            <  SRR19973077.4832796/150‑1 (MQ=60)
                                                                                                                                            CAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACG           <  SRR19973077.4222946/150‑1 (MQ=60)
                                                                                                                                            CAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACG           >  SRR19973077.1918401/1‑150 (MQ=60)
                                                                                                                                              AAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCC         <  SRR19973077.738965/150‑1 (MQ=60)
                                                                                                                                                   GTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCA    >  SRR19973077.4721586/1‑150 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  <  SRR19973077.4929925/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  <  SRR19973077.1847248/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  SRR19973077.1561212/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑