Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,020,166 C→A 85.7% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,020,1660CA85.7% 522.1 / 85.6 203D445Y (GAC→TAC) yedQputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base A (61/113);  total (76/127)
Fisher's exact test for biased strand distribution p-value = 9.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 204 total aligned reads displayed.

GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAC  >  CP009273/2020018‑2020314
                                                                                                                                                    |                                                                                                                                                    
 cAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAt                                                                                                                                                    <  2:1926874/150‑1 (MQ=255)
      tCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATc                                                                                                                                               <  2:1519848/150‑1 (MQ=255)
            aGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCACAGCGGTACTTAATCGCTTTa                                                                                                                                         >  2:4865904/1‑150 (MQ=255)
            aGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTa                                                                                                                                         <  2:4388294/150‑1 (MQ=255)
                tCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAt                                                                                                                                     <  2:4821616/150‑1 (MQ=255)
                   cgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATGGt                                                                                                                                  >  1:5072552/1‑150 (MQ=255)
                    gcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTc                                                                                                                                 <  1:1547856/150‑1 (MQ=255)
                    gcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTc                                                                                                                                 <  1:4430132/150‑1 (MQ=255)
                      cTGGCAGAATCACACAAAACTCCTCCCCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCaa                                                                                                                               <  2:3514504/150‑1 (MQ=255)
                         gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                            >  2:1794076/1‑150 (MQ=255)
                         gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                            <  2:5145237/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                           <  2:823550/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                           <  2:3746785/150‑1 (MQ=255)
                           aGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCg                                                                                                                          <  2:1351544/150‑1 (MQ=255)
                           aGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCg                                                                                                                          >  1:4693571/1‑150 (MQ=255)
                            gAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGa                                                                                                                         <  1:673178/150‑1 (MQ=255)
                              aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                       <  2:509331/150‑1 (MQ=255)
                              aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                       <  2:2959270/150‑1 (MQ=255)
                              aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                       >  2:1845497/1‑150 (MQ=255)
                               tCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCt                                                                                                                      <  1:5076404/150‑1 (MQ=255)
                                    caAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATg                                                                                                                 <  2:523152/150‑1 (MQ=255)
                                    caAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATg                                                                                                                 <  2:140294/150‑1 (MQ=255)
                                        aCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAg                                                                                                             >  2:860336/1‑150 (MQ=255)
                                         ctcctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCACAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGa                                                                                                            <  2:1322423/150‑1 (MQ=255)
                                          tcctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaa                                                                                                           <  2:4115032/150‑1 (MQ=255)
                                           cctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaa                                                                                                          <  1:4344655/150‑1 (MQ=255)
                                             tccccaccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaacaa                                                                                                        <  1:2296442/147‑1 (MQ=255)
                                                  accGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATg                                                                                                   >  1:4648469/1‑150 (MQ=255)
                                                    cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGACCGGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAGGGATGtt                                                                                                 >  2:1192192/1‑150 (MQ=255)
                                                    cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGtt                                                                                                 <  2:2466568/150‑1 (MQ=255)
                                                    cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGtt                                                                                                 <  1:3349247/150‑1 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAACAAGGATGTTg                                                                                                >  1:4005695/1‑150 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                                >  2:3398989/1‑150 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                                <  2:3663586/150‑1 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAACCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                                <  1:4240838/150‑1 (MQ=255)
                                                          gcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCg                                                                                           <  1:4123114/150‑1 (MQ=255)
                                                            ccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTc                                                                                         <  1:4943468/150‑1 (MQ=255)
                                                            ccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGACTGACAGAAAAAGGATGTTGGTGCGTc                                                                                         >  2:2793498/1‑150 (MQ=255)
                                                             cgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCt                                                                                        >  1:344963/1‑150 (MQ=255)
                                                              ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                       <  2:2777251/150‑1 (MQ=255)
                                                               gCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGa                                                                                      <  2:257807/150‑1 (MQ=255)
                                                                     tCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAt                                                                                >  1:2436756/1‑150 (MQ=255)
                                                                        tGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTa                                                                             <  2:1497524/150‑1 (MQ=255)
                                                                        tGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTa                                                                             <  1:3188193/150‑1 (MQ=255)
                                                                        tGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTa                                                                             <  1:3187984/150‑1 (MQ=255)
                                                                          cgcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagc                                                                           <  2:2875284/150‑1 (MQ=255)
                                                                          cgcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagc                                                                           <  1:2120008/150‑1 (MQ=255)
                                                                            cacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcga                                                                         <  1:3490176/150‑1 (MQ=255)
                                                                             acgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgag                                                                        <  2:3228865/150‑1 (MQ=255)
                                                                                 aaGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGa                                                                    >  1:531682/1‑150 (MQ=255)
                                                                                 aaGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGa                                                                    >  1:3140605/1‑150 (MQ=255)
                                                                                   ggAACTGCTAATTAAGCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACg                                                                  <  2:2794122/150‑1 (MQ=255)
                                                                                     aaatgataattaatCCGGCATCATGAGAAAGAACACGGTCGCCCGCCTGATGAACAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                <  1:2412160/144‑1 (MQ=255)
                                                                                      aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                               >  1:1957555/1‑150 (MQ=255)
                                                                                      aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                               >  2:3300320/1‑150 (MQ=255)
                                                                                      aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCGTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                               >  1:4545943/1‑150 (MQ=255)
                                                                                        tGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtt                                                             <  1:2148837/150‑1 (MQ=255)
                                                                                        tGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtt                                                             <  1:3787271/150‑1 (MQ=255)
                                                                                         gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttt                                                            <  1:2518874/150‑1 (MQ=255)
                                                                                            aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                         <  1:1647344/150‑1 (MQ=255)
                                                                                            aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                         >  1:2219998/1‑150 (MQ=255)
                                                                                             attaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCg                                                        >  2:2672428/1‑150 (MQ=255)
                                                                                               taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGaa                                                      >  1:1939698/1‑150 (MQ=255)
                                                                                               taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAATAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGaa                                                      <  2:776799/150‑1 (MQ=255)
                                                                                                aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                     >  2:1443509/1‑150 (MQ=255)
                                                                                                aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                     >  2:3523817/1‑150 (MQ=255)
                                                                                                aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                     <  1:3704406/150‑1 (MQ=255)
                                                                                                 atCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACa                                                    <  1:3533556/150‑1 (MQ=255)
                                                                                                        agcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                             >  2:2073014/1‑150 (MQ=255)
                                                                                                           aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                          <  1:273892/150‑1 (MQ=255)
                                                                                                           aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                          <  1:4147965/150‑1 (MQ=255)
                                                                                                             gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                        >  2:4663364/1‑150 (MQ=255)
                                                                                                             gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                        >  1:924466/1‑150 (MQ=255)
                                                                                                               gaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTta                                      >  2:3621686/1‑150 (MQ=255)
                                                                                                                aaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtat                                     <  2:1680169/150‑1 (MQ=255)
                                                                                                                 aataaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                    >  1:897104/4‑150 (MQ=255)
                                                                                                                 aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                    <  1:16282/150‑1 (MQ=255)
                                                                                                                 aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                    >  1:3160084/1‑150 (MQ=255)
                                                                                                                 aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                    <  1:2683087/150‑1 (MQ=255)
                                                                                                                  agaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatat                                   >  1:943959/1‑150 (MQ=255)
                                                                                                                     acacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaaa                                <  2:1330198/150‑1 (MQ=255)
                                                                                                                     acacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaaa                                <  2:3927790/150‑1 (MQ=255)
                                                                                                                      cacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAc                               >  2:1378070/1‑150 (MQ=255)
                                                                                                                          gTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAATAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGt                           <  1:2926632/150‑1 (MQ=255)
                                                                                                                              cccgccTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAg                       <  1:2804258/150‑1 (MQ=255)
                                                                                                                              cccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAg                       <  2:630779/150‑1 (MQ=255)
                                                                                                                                cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgt                     <  2:2188941/150‑1 (MQ=255)
                                                                                                                                 tacTGATTACCATAGCGTTATTTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAATAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtg                    <  1:1060957/148‑1 (MQ=255)
                                                                                                                                   cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc                  >  1:1677960/1‑150 (MQ=255)
                                                                                                                                   cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc                  <  1:396486/150‑1 (MQ=255)
                                                                                                                                    tgatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCg                 <  2:4333576/150‑1 (MQ=255)
                                                                                                                                     gatgaCCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt                <  1:1794076/150‑1 (MQ=255)
                                                                                                                                      atgaCCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTg               >  2:309804/1‑150 (MQ=255)
                                                                                                                                        gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACTGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAGAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc             <  2:2545724/150‑1 (MQ=255)
                                                                                                                                        gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc             >  2:3972622/1‑150 (MQ=255)
                                                                                                                                        gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGca             <  2:1165049/150‑2 (MQ=255)
                                                                                                                                           cAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACg          >  1:2583905/1‑150 (MQ=255)
                                                                                                                                              cgCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCt       <  1:1723632/149‑1 (MQ=255)
                                                                                                                                              aGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCt       <  2:3148824/150‑1 (MQ=255)
                                                                                                                                                   tCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAc  <  1:719510/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAC  >  CP009273/2020018‑2020314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973083.2452372/1‑150 (MQ=60)
 GCAGCCTCCGTCAGACTCGCGCCGGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAA                                                                                                                                                      <  SRR19973083.3782690/150‑1 (MQ=60)
  CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAT                                                                                                                                                     <  SRR19973083.3535741/150‑1 (MQ=60)
        CCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCG                                                                                                                                               <  SRR19973083.3797848/150‑1 (MQ=60)
             AGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTA                                                                                                                                          >  SRR19973083.92250/1‑150 (MQ=60)
                CTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAA                                                                                                                                       <  SRR19973083.1648253/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATGGT                                                                                                                                   >  SRR19973083.5094254/1‑150 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   >  SRR19973083.5095025/1‑150 (MQ=60)
                     GCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTC                                                                                                                                  <  SRR19973083.1555643/150‑1 (MQ=60)
                     GCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTC                                                                                                                                  <  SRR19973083.4449405/150‑1 (MQ=60)
                      CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCA                                                                                                                                 <  SRR19973083.470210/150‑1 (MQ=60)
                          GCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAT                                                                                                                             >  SRR19973083.2733907/1‑150 (MQ=60)
                            AGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCG                                                                                                                           >  SRR19973083.4713701/1‑150 (MQ=60)
                             GAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGA                                                                                                                          <  SRR19973083.676547/150‑1 (MQ=60)
                                TCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCT                                                                                                                       <  SRR19973083.5098118/150‑1 (MQ=60)
                                          CTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGA                                                                                                             <  SRR19973083.4495043/150‑1 (MQ=60)
                                            CCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAA                                                                                                           <  SRR19973083.4363477/150‑1 (MQ=60)
                                            CCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAA                                                                                                           <  SRR19973083.4363499/150‑1 (MQ=60)
                                              TCCCCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAACAA                                                                                                         <  SRR19973083.2308201/150‑1 (MQ=60)
                                              TCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAA                                                                                                         <  SRR19973083.2308101/150‑1 (MQ=60)
                                               CACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAG                                                                                                        >  SRR19973083.2117992/1‑150 (MQ=60)
                                                ACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGG                                                                                                       <  SRR19973083.2158492/150‑1 (MQ=60)
                                                  CACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAT                                                                                                     <  SRR19973083.1385621/150‑1 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    <  SRR19973083.3183468/150‑1 (MQ=60)
                                                   ACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATG                                                                                                    >  SRR19973083.4668495/1‑150 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973083.3364859/150‑1 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973083.5200732/150‑1 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAACAAGGATGTTG                                                                                                 >  SRR19973083.4023512/1‑150 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 <  SRR19973083.4259368/150‑1 (MQ=60)
                                                      GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAACCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 <  SRR19973083.4259397/150‑1 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             >  SRR19973083.3163010/1‑150 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             >  SRR19973083.3144669/1‑150 (MQ=60)
                                                           GCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCG                                                                                            <  SRR19973083.4141370/150‑1 (MQ=60)
                                                           GCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCG                                                                                            <  SRR19973083.4141352/150‑1 (MQ=60)
                                                           GCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCG                                                                                            <  SRR19973083.2195193/150‑1 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          <  SRR19973083.4965082/150‑1 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          <  SRR19973083.4964717/150‑1 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          >  SRR19973083.2525554/1‑150 (MQ=60)
                                                              CGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCT                                                                                         >  SRR19973083.346622/1‑150 (MQ=60)
                                                               GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTG                                                                                        >  SRR19973083.680112/1‑150 (MQ=60)
                                                               GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTG                                                                                        <  SRR19973083.4131745/150‑1 (MQ=60)
                                                                 CAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGAC                                                                                      >  SRR19973083.3957703/1‑150 (MQ=60)
                                                                      TCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAT                                                                                 >  SRR19973083.2449251/1‑150 (MQ=60)
                                                                         TGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTA                                                                              <  SRR19973083.3203156/150‑1 (MQ=60)
                                                                         TGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTA                                                                              <  SRR19973083.3203317/150‑1 (MQ=60)
                                                                         TGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTA                                                                              <  SRR19973083.3203367/150‑1 (MQ=60)
                                                                           CGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGC                                                                            <  SRR19973083.2130706/150‑1 (MQ=60)
                                                                             CACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGA                                                                          >  SRR19973083.4794665/1‑150 (MQ=60)
                                                                             CACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGA                                                                          <  SRR19973083.3506149/150‑1 (MQ=60)
                                                                                GCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCG                                                                       <  SRR19973083.2433482/150‑1 (MQ=60)
                                                                                  AAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     >  SRR19973083.3155624/1‑150 (MQ=60)
                                                                                  AAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGA                                                                     >  SRR19973083.534312/1‑150 (MQ=60)
                                                                                      AACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 >  SRR19973083.1744971/1‑150 (MQ=60)
                                                                                      AAATGATAATTAATCCGGCATCATGAGAAAGAACACGGTCGCCCGCCTGATGAACAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGG                                                                 <  SRR19973083.2424556/150‑1 (MQ=60)
                                                                                       ACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGC                                                                >  SRR19973083.1967562/1‑150 (MQ=60)
                                                                                       ACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCGTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGC                                                                >  SRR19973083.4565649/1‑150 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973083.3804350/150‑1 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973083.2159639/150‑1 (MQ=60)
                                                                                          GCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTT                                                             <  SRR19973083.2531677/150‑1 (MQ=60)
                                                                                          GCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTT                                                             <  SRR19973083.2531687/150‑1 (MQ=60)
                                                                                             AATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          <  SRR19973083.1655539/150‑1 (MQ=60)
                                                                                             AATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          >  SRR19973083.2231216/1‑150 (MQ=60)
                                                                                             AATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          >  SRR19973083.3252175/1‑150 (MQ=60)
                                                                                             AATTAATCATGCTGCATGAGAATGATCACGGTCGCCCGCCTGATGACCTAAGCGGTAATTAATCGATTTAAAATGTTCAAGATCGAACTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          <  SRR19973083.2389662/150‑1 (MQ=60)
                                                                                               TTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGA                                                        >  SRR19973083.2901634/1‑150 (MQ=60)
                                                                                                TAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAA                                                       >  SRR19973083.1949606/1‑150 (MQ=60)
                                                                                                 AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      <  SRR19973083.3721275/150‑1 (MQ=60)
                                                                                                  ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     <  SRR19973083.3549776/150‑1 (MQ=60)
                                                                                                   TCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAG                                                    <  SRR19973083.666648/150‑1 (MQ=60)
                                                                                                      GGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGC                                                 >  SRR19973083.363213/1‑150 (MQ=60)
                                                                                                      GGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTCCGAACAGTGC                                                 <  SRR19973083.3468088/150‑1 (MQ=60)
                                                                                                           CATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAC                                            >  SRR19973083.766510/1‑150 (MQ=60)
                                                                                                            ATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACG                                           >  SRR19973083.3455486/1‑150 (MQ=60)
                                                                                                            ATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACG                                           <  SRR19973083.4166284/150‑1 (MQ=60)
                                                                                                            ATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACG                                           <  SRR19973083.275339/150‑1 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         >  SRR19973083.928986/1‑150 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         <  SRR19973083.1585100/150‑1 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         >  SRR19973083.928940/1‑150 (MQ=60)
                                                                                                              GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         <  SRR19973083.464035/150‑1 (MQ=60)
                                                                                                                  AATAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     >  SRR19973083.901362/1‑150 (MQ=60)
                                                                                                                  AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     <  SRR19973083.2696539/150‑1 (MQ=60)
                                                                                                                  AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     >  SRR19973083.3175159/1‑150 (MQ=60)
                                                                                                                  AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     <  SRR19973083.16422/150‑1 (MQ=60)
                                                                                                                   AGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAACAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATAT                                    >  SRR19973083.948574/1‑150 (MQ=60)
                                                                                                                       CACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAC                                <  SRR19973083.1953624/150‑1 (MQ=60)
                                                                                                                         CGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGC                              >  SRR19973083.402157/1‑150 (MQ=60)
                                                                                                                           GTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAATAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGT                            <  SRR19973083.2940942/150‑1 (MQ=60)
                                                                                                                           GTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGT                            <  SRR19973083.2613453/150‑1 (MQ=60)
                                                                                                                              GCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTGATATAAACGCGTTAA                         >  SRR19973083.4447647/1‑150 (MQ=60)
                                                                                                                               CCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAG                        <  SRR19973083.2818170/150‑1 (MQ=60)
                                                                                                                                   CCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGT                    >  SRR19973083.974631/1‑150 (MQ=60)
                                                                                                                                    CTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTC                   >  SRR19973083.1686238/1‑150 (MQ=60)
                                                                                                                                    CTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTC                   >  SRR19973083.1686258/1‑150 (MQ=60)
                                                                                                                                    CTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTC                   <  SRR19973083.398292/150‑1 (MQ=60)
                                                                                                                                      GATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGT                 <  SRR19973083.1803045/150‑1 (MQ=60)
                                                                                                                                            CAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACG           >  SRR19973083.2596918/1‑150 (MQ=60)
                                                                                                                                               CGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT        <  SRR19973083.1732095/150‑1 (MQ=60)
                                                                                                                                               AGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT        <  SRR19973083.1732029/150‑1 (MQ=60)
                                                                                                                                                  GGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC     <  SRR19973083.1848859/150‑1 (MQ=60)
                                                                                                                                                  GGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC     <  SRR19973083.2808750/150‑1 (MQ=60)
                                                                                                                                                   GTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCA    <  SRR19973083.1080789/150‑1 (MQ=60)
                                                                                                                                                    TCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAC   <  SRR19973083.723072/150‑1 (MQ=60)
                                                                                                                                                    TCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAC   <  SRR19973083.3339784/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  <  SRR19973083.343243/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑