Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,020,166 C→A 94.4% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,020,1660CA94.4% 722.2 / 30.0 232D445Y (GAC→TAC) yedQputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (97/122);  total (104/128)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 233 total aligned reads displayed.

CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTG  >  CP009273/2020019‑2020310
                                                                                                                                                   |                                                                                                                                                
cAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAt                                                                                                                                                <  2:142603/150‑1 (MQ=255)
 aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                               <  1:3093708/150‑1 (MQ=255)
 aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                               <  1:3093234/150‑1 (MQ=255)
 aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                               <  2:3560092/150‑1 (MQ=255)
    cTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGtaattaat                                                                                                                                            >  2:78683/1‑150 (MQ=255)
     tCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATc                                                                                                                                           <  1:2230090/150‑1 (MQ=255)
      ccGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCg                                                                                                                                          <  2:320155/150‑1 (MQ=255)
       cGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGc                                                                                                                                         >  1:4286690/1‑150 (MQ=255)
       cGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGc                                                                                                                                         >  1:387656/1‑150 (MQ=255)
        gTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCt                                                                                                                                        <  2:661873/150‑1 (MQ=255)
           aGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTa                                                                                                                                     >  1:855743/1‑150 (MQ=255)
               tCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAt                                                                                                                                 <  1:2875380/150‑1 (MQ=255)
                 gcgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATgg                                                                                                                               <  1:1674135/150‑1 (MQ=255)
                  cgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGt                                                                                                                              >  1:3430705/1‑150 (MQ=255)
                   gcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTc                                                                                                                             >  1:2096889/1‑150 (MQ=255)
                    ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCa                                                                                                                            <  2:3215439/150‑1 (MQ=255)
                    ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCa                                                                                                                            >  2:2660267/1‑150 (MQ=255)
                    ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCa                                                                                                                            >  2:486729/1‑150 (MQ=255)
                     cTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCaa                                                                                                                           >  1:3419305/1‑150 (MQ=255)
                        gctgAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                        <  2:4014595/147‑1 (MQ=255)
                        gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                        <  2:1824400/150‑1 (MQ=255)
                        gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                        <  2:1149852/150‑1 (MQ=255)
                        gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                        <  2:4318983/150‑1 (MQ=255)
                         cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                       >  2:3648979/1‑150 (MQ=255)
                            aaTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGAc                                                                                                                    <  2:619478/150‑1 (MQ=255)
                            aaTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAc                                                                                                                    >  2:59479/1‑150 (MQ=255)
                             aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                   >  1:2750427/1‑150 (MQ=255)
                             aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                   <  1:3233253/150‑1 (MQ=255)
                              tCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCt                                                                                                                  <  2:805941/150‑1 (MQ=255)
                              tCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCt                                                                                                                  <  2:3076382/150‑1 (MQ=255)
                                  acaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAt                                                                                                              >  1:934412/1‑150 (MQ=255)
                                   caAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAACGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATg                                                                                                             <  2:2522723/150‑1 (MQ=255)
                                    aaaaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGa                                                                                                            >  1:1357676/1‑150 (MQ=255)
                                      aaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACa                                                                                                          <  2:1962605/150‑1 (MQ=255)
                                        ctactcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTAAAGATCGACCTGAATGACAGa                                                                                                        >  2:747213/4‑150 (MQ=255)
                                             caccacCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAg                                                                                                   >  1:3610417/1‑150 (MQ=255)
                                                  ccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGt                                                                                              <  2:3450985/150‑1 (MQ=255)
                                                  ccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGt                                                                                              <  1:613068/150‑1 (MQ=255)
                                                  ccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGATAATTACTCCGGCAGCATGAGAAAGAACACGGTCGCCCTCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGt                                                                                              <  1:3409405/150‑1 (MQ=255)
                                                   cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGtt                                                                                             <  2:89250/150‑1 (MQ=255)
                                                    gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                            <  1:3590946/150‑1 (MQ=255)
                                                    gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                            >  1:165659/1‑150 (MQ=255)
                                                    gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                            >  2:2676828/1‑150 (MQ=255)
                                                      cccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGt                                                                                          >  2:3746223/1‑150 (MQ=255)
                                                       ccgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTg                                                                                         <  2:2862416/150‑1 (MQ=255)
                                                        cgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGc                                                                                        <  1:1163498/150‑1 (MQ=255)
                                                         gcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCg                                                                                       >  1:1689903/1‑150 (MQ=255)
                                                            cgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCt                                                                                    <  1:2059581/150‑1 (MQ=255)
                                                                aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                                >  2:336275/1‑150 (MQ=255)
                                                                  cGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGAcaca                                                                              <  1:3331395/150‑1 (MQ=255)
                                                                      cTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAGGGATGTTGGTGCGTCTGACACAAttt                                                                          >  1:869745/1‑150 (MQ=255)
                                                                      cTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAttt                                                                          <  1:729512/150‑1 (MQ=255)
                                                                      cTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAttt                                                                          <  1:2818874/150‑1 (MQ=255)
                                                                        gcgcACGCAGGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTag                                                                        <  2:2407897/150‑1 (MQ=255)
                                                                          gcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcg                                                                      <  2:855743/150‑1 (MQ=255)
                                                                             cgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgagc                                                                   >  2:3938178/1‑150 (MQ=255)
                                                                              gcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgagcg                                                                  <  2:1688686/150‑1 (MQ=255)
                                                                                   gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                             >  1:1198409/1‑150 (MQ=255)
                                                                                   gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACgg                                                             <  1:267373/150‑1 (MQ=255)
                                                                                    aaCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                            >  2:3320164/1‑150 (MQ=255)
                                                                                     aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                           >  1:496279/1‑150 (MQ=255)
                                                                                      cTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCt                                                          >  1:3602917/1‑150 (MQ=255)
                                                                                        gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttt                                                        <  2:2308396/150‑1 (MQ=255)
                                                                                        gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttt                                                        >  2:426483/1‑150 (MQ=255)
                                                                                        gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCttt                                                        >  2:3189794/1‑150 (MQ=255)
                                                                                           aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                     >  1:1913717/1‑150 (MQ=255)
                                                                                               aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                 >  1:1875677/1‑150 (MQ=255)
                                                                                               aatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                 >  1:3362948/1‑150 (MQ=255)
                                                                                                atCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACa                                                <  1:4170382/150‑1 (MQ=255)
                                                                                                  ccGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGt                                              <  1:1018165/150‑1 (MQ=255)
                                                                                                   cGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                             >  1:3868658/1‑150 (MQ=255)
                                                                                                   cGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                             <  1:4248646/150‑1 (MQ=255)
                                                                                                    ggCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgc                                            >  2:408042/1‑150 (MQ=255)
                                                                                                     gcagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcg                                           <  1:2336755/150‑1 (MQ=255)
                                                                                                         caagagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAc                                       <  2:4182287/147‑1 (MQ=255)
                                                                                                          aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                      <  2:1292192/150‑1 (MQ=255)
                                                                                                          aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACg                                      >  2:2035904/1‑150 (MQ=255)
                                                                                                            gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                    <  1:2442584/150‑1 (MQ=255)
                                                                                                                aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                >  2:3607334/1‑150 (MQ=255)
                                                                                                                 agaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatat                               <  2:3617325/150‑1 (MQ=255)
                                                                                                                  gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatata                              <  2:3892170/150‑1 (MQ=255)
                                                                                                                     cacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAc                           <  1:284218/150‑1 (MQ=255)
                                                                                                                     cacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAc                           >  1:4339157/1‑150 (MQ=255)
                                                                                                                      acGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcg                          <  2:1200143/150‑1 (MQ=255)
                                                                                                                        ggTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgcg                        <  1:1029483/150‑1 (MQ=255)
                                                                                                                          tCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGtt                      <  1:273906/150‑1 (MQ=255)
                                                                                                                            gcccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTaa                    <  1:2983597/150‑1 (MQ=255)
                                                                                                                             cccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAg                   <  2:1046897/150‑1 (MQ=255)
                                                                                                                              ccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAgg                  >  2:2451978/1‑150 (MQ=255)
                                                                                                                               cgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgt                 <  1:1031166/150‑1 (MQ=255)
                                                                                                                                gccTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtg                >  2:3812895/1‑150 (MQ=255)
                                                                                                                                  cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc              <  2:3726509/150‑1 (MQ=255)
                                                                                                                                  cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc              <  2:84708/150‑1 (MQ=255)
                                                                                                                                       gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc         >  2:2621279/1‑150 (MQ=255)
                                                                                                                                        aCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGTGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCa        >  1:1116482/1‑150 (MQ=255)
                                                                                                                                        aCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCa        >  1:1864745/1‑150 (MQ=255)
                                                                                                                                           aaaGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGc     >  1:3898905/1‑150 (MQ=255)
                                                                                                                                           aaaGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGc     >  1:2001405/1‑150 (MQ=255)
                                                                                                                                             aGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCt   <  2:3043741/150‑1 (MQ=255)
                                                                                                                                              gCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTg  <  2:1481863/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTG  >  CP009273/2020019‑2020310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020020‑2020315
                                                                                                                                                  |                                                                                                                                                     
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973079.3109221/150‑1 (MQ=60)
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973079.3109302/150‑1 (MQ=60)
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973079.3109696/150‑1 (MQ=60)
 GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973079.433571/150‑1 (MQ=60)
 GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973079.3652/150‑1 (MQ=60)
 GCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTA                                                                                                                                                   <  SRR19973079.3539/150‑1 (MQ=60)
  CCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAA                                                                                                                                                  >  SRR19973079.1697768/1‑150 (MQ=60)
    TCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATC                                                                                                                                                <  SRR19973079.2241622/150‑1 (MQ=60)
      CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              <  SRR19973079.2915830/150‑1 (MQ=60)
      CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              >  SRR19973079.389959/1‑150 (MQ=60)
      CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              >  SRR19973079.4308324/1‑150 (MQ=60)
      CGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGC                                                                                                                                              >  SRR19973079.389865/1‑150 (MQ=60)
       GTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCT                                                                                                                                             <  SRR19973079.3485442/150‑1 (MQ=60)
       GTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCT                                                                                                                                             <  SRR19973079.1280114/150‑1 (MQ=60)
        TCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTT                                                                                                                                            <  SRR19973079.3155636/150‑1 (MQ=60)
        TCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTT                                                                                                                                            >  SRR19973079.3863514/1‑150 (MQ=60)
          AGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTA                                                                                                                                          >  SRR19973079.860688/1‑150 (MQ=60)
          AGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTA                                                                                                                                          <  SRR19973079.2792272/150‑1 (MQ=60)
              TCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAAT                                                                                                                                      >  SRR19973079.2802533/1‑150 (MQ=60)
              TCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAT                                                                                                                                      <  SRR19973079.2889943/150‑1 (MQ=60)
               CGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTACTTAATCGCTTTAAAATG                                                                                                                                     >  SRR19973079.3049671/1‑150 (MQ=60)
                GCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGG                                                                                                                                    <  SRR19973079.1683150/150‑1 (MQ=60)
                GCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGG                                                                                                                                    <  SRR19973079.1683168/150‑1 (MQ=60)
                 CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   >  SRR19973079.3448419/1‑150 (MQ=60)
                 CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   >  SRR19973079.2005476/1‑150 (MQ=60)
                  GCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTC                                                                                                                                  >  SRR19973079.2107793/1‑150 (MQ=60)
                   CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCTCTTTAAAATGGTCA                                                                                                                                 >  SRR19973079.4200975/1‑150 (MQ=60)
                    CTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAA                                                                                                                                >  SRR19973079.3437312/1‑150 (MQ=60)
                    CTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAA                                                                                                                                >  SRR19973079.3436961/1‑150 (MQ=60)
                    CTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAA                                                                                                                                >  SRR19973079.240660/1‑150 (MQ=60)
                          GAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGA                                                                                                                          >  SRR19973079.1471240/1‑150 (MQ=60)
                           AATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAC                                                                                                                         >  SRR19973079.2320372/1‑150 (MQ=60)
                            ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        <  SRR19973079.3249200/150‑1 (MQ=60)
                            ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        >  SRR19973079.2764601/1‑150 (MQ=60)
                            ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        >  SRR19973079.2764602/1‑150 (MQ=60)
                            ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        <  SRR19973079.3249650/150‑1 (MQ=60)
                             TAACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCT                                                                                                                       >  SRR19973079.906971/1‑150 (MQ=60)
                                 ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   >  SRR19973079.939788/1‑150 (MQ=60)
                                 ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   >  SRR19973079.939777/1‑150 (MQ=60)
                                 ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   >  SRR19973079.1915994/1‑150 (MQ=60)
                                   AAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGA                                                                                                                 >  SRR19973079.1365240/1‑150 (MQ=60)
                                      ACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAG                                                                                                              <  SRR19973079.4115557/150‑1 (MQ=60)
                                       CTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGA                                                                                                             >  SRR19973079.2887258/1‑150 (MQ=60)
                                        TCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAA                                                                                                            <  SRR19973079.4326606/150‑1 (MQ=60)
                                         CCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAA                                                                                                           >  SRR19973079.2490370/1‑150 (MQ=60)
                                         CCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAA                                                                                                           >  SRR19973079.2490382/1‑150 (MQ=60)
                                            CACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAG                                                                                                        >  SRR19973079.3628716/1‑150 (MQ=60)
                                            CACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAG                                                                                                        <  SRR19973079.1870639/150‑1 (MQ=60)
                                             ACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGG                                                                                                       >  SRR19973079.3429115/1‑150 (MQ=60)
                                               CACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAT                                                                                                     >  SRR19973079.54742/1‑150 (MQ=60)
                                                 CCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGT                                                                                                   <  SRR19973079.616612/150‑1 (MQ=60)
                                                 CCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGATAATTACTCCGGCAGCATGAGAAAGAACACGGTCGCCCTCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGT                                                                                                   <  SRR19973079.3427004/150‑1 (MQ=60)
                                                  CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973079.713805/150‑1 (MQ=60)
                                                   GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973079.2845629/1‑150 (MQ=60)
                                                   GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 >  SRR19973079.166920/1‑150 (MQ=60)
                                                   GACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTG                                                                                                 <  SRR19973079.3609208/150‑1 (MQ=60)
                                                       CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             <  SRR19973079.1169864/150‑1 (MQ=60)
                                                       CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             <  SRR19973079.1170200/150‑1 (MQ=60)
                                                        GCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCG                                                                                            >  SRR19973079.1698988/1‑150 (MQ=60)
                                                           CGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCT                                                                                         <  SRR19973079.2070462/150‑1 (MQ=60)
                                                           CGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCT                                                                                         <  SRR19973079.2070378/150‑1 (MQ=60)
                                                                 CGTCCTGCGCACGCAAGGAACTGATAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACA                                                                                   <  SRR19973079.3348459/150‑1 (MQ=60)
                                                                     CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCTACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACTCAATTT                                                                               <  SRR19973079.4279577/150‑1 (MQ=60)
                                                                     CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAGGGATGTTGGTGCGTCTGACACAATTT                                                                               >  SRR19973079.874710/1‑150 (MQ=60)
                                                                     CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAGGGATGTTGGTGCGTCTGACACAATTT                                                                               >  SRR19973079.874776/1‑150 (MQ=60)
                                                                     CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTT                                                                               <  SRR19973079.733368/150‑1 (MQ=60)
                                                                     CTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTT                                                                               <  SRR19973079.2833292/150‑1 (MQ=60)
                                                                        CGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGC                                                                            <  SRR19973079.1372784/150‑1 (MQ=60)
                                                                                  GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  <  SRR19973079.269072/150‑1 (MQ=60)
                                                                                  GAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGG                                                                  >  SRR19973079.1205303/1‑150 (MQ=60)
                                                                                    ACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGC                                                                >  SRR19973079.499305/1‑150 (MQ=60)
                                                                                     CTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCT                                                               >  SRR19973079.3621210/1‑150 (MQ=60)
                                                                                        CTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTT                                                            >  SRR19973079.3871762/1‑150 (MQ=60)
                                                                                         TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           >  SRR19973079.2131282/1‑150 (MQ=60)
                                                                                          AATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTC                                                          >  SRR19973079.1923931/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.830927/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.1024167/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.3379931/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.3296872/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.1885649/1‑150 (MQ=60)
                                                                                              AATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGAGAGCGGACGGGCTTTTTCGAAC                                                      >  SRR19973079.3380254/1‑150 (MQ=60)
                                                                                               ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     <  SRR19973079.1175462/150‑1 (MQ=60)
                                                                                               ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     >  SRR19973079.3615525/1‑150 (MQ=60)
                                                                                               ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     <  SRR19973079.4191529/150‑1 (MQ=60)
                                                                                                 CCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGT                                                   <  SRR19973079.1023867/150‑1 (MQ=60)
                                                                                                  CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  <  SRR19973079.4270142/150‑1 (MQ=60)
                                                                                                  CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  <  SRR19973079.2069619/150‑1 (MQ=60)
                                                                                                  CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  >  SRR19973079.3888598/1‑150 (MQ=60)
                                                                                                  CGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTG                                                  >  SRR19973079.3888344/1‑150 (MQ=60)
                                                                                                    GCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCG                                                <  SRR19973079.2348892/150‑1 (MQ=60)
                                                                                                       GCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCA                                             <  SRR19973079.3412099/150‑1 (MQ=60)
                                                                                                           GAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGT                                         <  SRR19973079.2455131/150‑1 (MQ=60)
                                                                                                            AGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTT                                        <  SRR19973079.1707451/150‑1 (MQ=60)
                                                                                                               AAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATA                                     <  SRR19973079.1040194/150‑1 (MQ=60)
                                                                                                                    CACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAC                                <  SRR19973079.285965/150‑1 (MQ=60)
                                                                                                                    CACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAC                                >  SRR19973079.4360935/1‑150 (MQ=60)
                                                                                                                       GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             >  SRR19973079.3396114/1‑150 (MQ=60)
                                                                                                                       GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             <  SRR19973079.1035211/150‑1 (MQ=60)
                                                                                                                       GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             <  SRR19973079.1036155/150‑1 (MQ=60)
                                                                                                                         TCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTT                           <  SRR19973079.275623/150‑1 (MQ=60)
                                                                                                                         TCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTT                           <  SRR19973079.275523/150‑1 (MQ=60)
                                                                                                                           GCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAA                         <  SRR19973079.2998965/150‑1 (MQ=60)
                                                                                                                            CCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAG                        >  SRR19973079.2937102/1‑150 (MQ=60)
                                                                                                                            CCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAG                        <  SRR19973079.2122571/150‑1 (MQ=60)
                                                                                                                              CGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGT                      <  SRR19973079.1036898/150‑1 (MQ=60)
                                                                                                                                CCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGT                    <  SRR19973079.4371739/150‑1 (MQ=60)
                                                                                                                                      GACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCC              >  SRR19973079.2002962/1‑150 (MQ=60)
                                                                                                                                       ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGTGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973079.1124350/1‑150 (MQ=60)
                                                                                                                                       ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGTGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973079.1122795/1‑150 (MQ=60)
                                                                                                                                       ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973079.1874752/1‑150 (MQ=60)
                                                                                                                                       ACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCA             >  SRR19973079.1874635/1‑150 (MQ=60)
                                                                                                                                          AAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGC          >  SRR19973079.3918685/1‑150 (MQ=60)
                                                                                                                                          AAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAAGACAGAAAAAGGATGTTGGAGCGTCTGACACAATTTAGCGAGCGGACGGGCTTATTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGC          >  SRR19973079.3918725/1‑150 (MQ=60)
                                                                                                                                          AAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGC          >  SRR19973079.2011997/1‑150 (MQ=60)
                                                                                                                                            AGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT        >  SRR19973079.644500/1‑150 (MQ=60)
                                                                                                                                                GTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCA    <  SRR19973079.3072329/150‑1 (MQ=60)
                                                                                                                                                GTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGATAAGGTGTCGTGCCACGCCTGCCA    <  SRR19973079.2690703/150‑1 (MQ=60)
                                                                                                                                                  AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  SRR19973079.2443010/1‑150 (MQ=60)
                                                                                                                                                  AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  SRR19973079.44080/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020020‑2020315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑