Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,020,166 C→A 88.8% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,020,1660CA88.8% 661.0 / 79.9 241D445Y (GAC→TAC) yedQputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (11/16);  new base A (90/124);  total (101/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 242 total aligned reads displayed.

GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC  >  CP009273/2020018‑2020312
                                                                                                                                                    |                                                                                                                                                  
  aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                                 <  2:310816/150‑1 (MQ=255)
  aGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAAtt                                                                                                                                                 <  2:2576147/150‑1 (MQ=255)
    ccTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTaa                                                                                                                                               <  1:3403172/150‑1 (MQ=255)
         gTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAAATAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCt                                                                                                                                          >  2:3711373/1‑150 (MQ=255)
            aGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTa                                                                                                                                       >  1:2634488/1‑150 (MQ=255)
             gACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTaa                                                                                                                                      >  1:987697/1‑150 (MQ=255)
               cTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTaaaa                                                                                                                                    >  1:2399909/1‑150 (MQ=255)
                tCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAt                                                                                                                                   <  2:1865620/150‑1 (MQ=255)
                tCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAAt                                                                                                                                   >  2:52542/1‑150 (MQ=255)
                 cgcgCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATg                                                                                                                                  >  2:79823/1‑150 (MQ=255)
                  gcgcCTGGCAGAATCACACACAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATgg                                                                                                                                 <  2:3957907/150‑1 (MQ=255)
                  gcgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATgg                                                                                                                                 <  2:1680003/150‑1 (MQ=255)
                   cgcCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGt                                                                                                                                <  1:4330/150‑1 (MQ=255)
                     ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCa                                                                                                                              <  1:3056564/150‑1 (MQ=255)
                     ccTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCa                                                                                                                              <  1:4052716/150‑1 (MQ=255)
                      cTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCaa                                                                                                                             >  2:1639133/1‑150 (MQ=255)
                      cTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCaa                                                                                                                             >  1:2906288/1‑150 (MQ=255)
                        ggCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGa                                                                                                                           >  2:2495801/1‑150 (MQ=255)
                        ggCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGa                                                                                                                           >  2:708918/1‑150 (MQ=255)
                         gCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGAt                                                                                                                          <  2:3759032/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                         <  2:1449154/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                         <  2:4490862/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                         >  1:2432964/1‑150 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                         <  1:2669369/150‑1 (MQ=255)
                          cAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATc                                                                                                                         <  2:2258580/150‑1 (MQ=255)
                           aGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCg                                                                                                                        >  1:3207825/1‑150 (MQ=255)
                            gAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGa                                                                                                                       <  2:148175/150‑1 (MQ=255)
                              aTCACACACAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                     <  2:3344295/150‑1 (MQ=255)
                              aTCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGAcc                                                                                                                     <  1:1812436/150‑1 (MQ=255)
                                cacacaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTg                                                                                                                   <  2:2130845/150‑1 (MQ=255)
                                 acacaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGa                                                                                                                  >  1:1069470/1‑150 (MQ=255)
                                   acaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAt                                                                                                                <  1:1596674/150‑1 (MQ=255)
                                   acaAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGATAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGTTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAt                                                                                                                <  1:3871181/150‑1 (MQ=255)
                                      aaaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGAc                                                                                                             <  1:3751275/150‑1 (MQ=255)
                                      aaaCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGAc                                                                                                             <  1:1132645/150‑1 (MQ=255)
                                        aCTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAg                                                                                                           >  1:1050603/1‑150 (MQ=255)
                                         ctcctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGa                                                                                                          >  2:2932502/1‑150 (MQ=255)
                                          acctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaa                                                                                                         >  2:621635/2‑150 (MQ=255)
                                           cctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaa                                                                                                        >  2:4100075/1‑150 (MQ=255)
                                            ctcACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGaaaa                                                                                                       >  1:685616/1‑150 (MQ=255)
                                               accaccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAgg                                                                                                    <  2:60217/150‑1 (MQ=255)
                                                ccaccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGa                                                                                                   >  2:486543/1‑150 (MQ=255)
                                                 caccGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGAt                                                                                                  >  2:4304383/1‑150 (MQ=255)
                                                    cGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGtt                                                                                               >  2:2040319/1‑150 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                              >  2:4117842/1‑150 (MQ=255)
                                                     gACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTg                                                                                              >  2:4373804/1‑150 (MQ=255)
                                                         cgcccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGc                                                                                          >  2:2148555/1‑150 (MQ=255)
                                                           cccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGt                                                                                        >  2:754203/1‑150 (MQ=255)
                                                            ccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTc                                                                                       >  1:3419946/1‑150 (MQ=255)
                                                            ccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTc                                                                                       >  1:219394/1‑150 (MQ=255)
                                                            ccgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTc                                                                                       <  2:4390658/150‑1 (MQ=255)
                                                             cgGCAACGTCCTGCTCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGtat                                                                                      <  1:938806/150‑3 (MQ=255)
                                                             cgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCt                                                                                      >  2:2850171/1‑150 (MQ=255)
                                                             cgGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCt                                                                                      <  2:1713801/150‑1 (MQ=255)
                                                              ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                     >  2:3892968/1‑150 (MQ=255)
                                                              ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTg                                                                                     <  2:274524/150‑1 (MQ=255)
                                                                 aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGaca                                                                                  >  1:3091876/1‑150 (MQ=255)
                                                                     tCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAt                                                                              <  1:3140302/150‑1 (MQ=255)
                                                                         gcgcACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTag                                                                          <  1:3865506/150‑1 (MQ=255)
                                                                          cgcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagc                                                                         >  2:3053316/1‑150 (MQ=255)
                                                                            cacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcga                                                                       >  1:1242054/1‑150 (MQ=255)
                                                                             acgcaAGGAACTGATAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCTGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTagcgag                                                                      <  2:2444487/150‑1 (MQ=255)
                                                                                caAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCgg                                                                   <  2:2273655/150‑1 (MQ=255)
                                                                                caAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCgg                                                                   >  2:3435032/1‑150 (MQ=255)
                                                                                  aGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAc                                                                 <  1:2548032/150‑1 (MQ=255)
                                                                                      aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                             <  2:3867288/150‑1 (MQ=255)
                                                                                      aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGc                                                             <  2:3549814/150‑1 (MQ=255)
                                                                                        tGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtt                                                           <  1:1962417/150‑1 (MQ=255)
                                                                                          cTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtttt                                                         >  1:173668/1‑150 (MQ=255)
                                                                                            aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                       >  2:223178/1‑150 (MQ=255)
                                                                                            aattaatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTc                                                       <  2:1871120/150‑1 (MQ=255)
                                                                                                   ccGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGAGGACCAACGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTGAGCGAGCGGACGGGCTTTTTCGAACAGt                                                <  2:2094307/150‑1 (MQ=255)
                                                                                                    cGGCAGCATGAGAAAGAACACGGTCGCCCGCCAGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                               <  2:902428/150‑1 (MQ=255)
                                                                                                      gcagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcg                                             <  2:3238168/150‑1 (MQ=255)
                                                                                                       cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcgc                                            >  2:3117659/1‑150 (MQ=255)
                                                                                                          caTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAc                                         <  2:1118719/150‑1 (MQ=255)
                                                                                                          caTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCAc                                         <  2:2432964/150‑1 (MQ=255)
                                                                                                             gagaAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGt                                      >  2:1816338/1‑150 (MQ=255)
                                                                                                               gaaagaaCACGGTCGCCCGCCTGTTGACCAAAGCGGTAATTAATCGCTTTAATATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAGGGGCTTTTTCGACCAGTGCGCCACGGTta                                    <  1:1038832/150‑1 (MQ=255)
                                                                                                               gaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTta                                    >  1:4301370/1‑150 (MQ=255)
                                                                                                               gaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTta                                    <  1:4417722/150‑1 (MQ=255)
                                                                                                               gaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTta                                    <  1:142401/150‑1 (MQ=255)
                                                                                                                  agaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtatat                                 >  1:4228725/1‑150 (MQ=255)
                                                                                                                     acacGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaaa                              >  2:3887392/1‑150 (MQ=255)
                                                                                                                         ggTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgcg                          <  2:3438357/150‑1 (MQ=255)
                                                                                                                         ggTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgcg                          >  1:3330874/1‑150 (MQ=255)
                                                                                                                           tCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGtt                        <  2:1827765/150‑1 (MQ=255)
                                                                                                                              cccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAg                     >  1:1639020/1‑150 (MQ=255)
                                                                                                                               ccgccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAgg                    <  1:52542/150‑1 (MQ=255)
                                                                                                                                cgccTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTGATATAAACGCGTTAAGgt                   >  2:1242010/1‑150 (MQ=255)
                                                                                                                                 gccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtg                  <  1:3983926/150‑1 (MQ=255)
                                                                                                                                 gccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtg                  <  1:2015238/150‑1 (MQ=255)
                                                                                                                                  ccTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGAGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGgtgt                 >  2:1093163/1‑150 (MQ=255)
                                                                                                                                   cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc                >  2:3970107/1‑150 (MQ=255)
                                                                                                                                   cTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTGATATAAACGCGTTAAGGTGTc                >  2:2769454/1‑150 (MQ=255)
                                                                                                                                     gatgaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt              >  2:3417330/1‑150 (MQ=255)
                                                                                                                                        gaCCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc           <  1:2684029/150‑1 (MQ=255)
                                                                                                                                          ccAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCAc         >  1:4426705/1‑150 (MQ=255)
                                                                                                                                           cAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACg        <  1:4438068/150‑1 (MQ=255)
                                                                                                                                                 ggTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGcc  <  1:3259851/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC  >  CP009273/2020018‑2020312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973080.3912520/1‑150 (MQ=60)
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973080.781140/1‑150 (MQ=60)
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTA                                                                                                                                                       >  SRR19973080.781555/1‑150 (MQ=60)
 GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCA                                                                                                                                                      <  SRR19973080.4420296/150‑1 (MQ=60)
 GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCA                                                                                                                                                      <  SRR19973080.1208126/150‑1 (MQ=60)
 GCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAA                                                                                                                                                      <  SRR19973080.1549513/150‑1 (MQ=60)
   AGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATT                                                                                                                                                    <  SRR19973080.1621453/150‑1 (MQ=60)
     CCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAA                                                                                                                                                  <  SRR19973080.3421562/150‑1 (MQ=60)
     CCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAA                                                                                                                                                  <  SRR19973080.3421397/150‑1 (MQ=60)
      CTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAAT                                                                                                                                                 <  SRR19973080.779801/150‑1 (MQ=60)
          GTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCT                                                                                                                                             >  SRR19973080.1109077/1‑150 (MQ=60)
             AGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTA                                                                                                                                          >  SRR19973080.2648728/1‑150 (MQ=60)
             AGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTA                                                                                                                                          <  SRR19973080.439975/150‑1 (MQ=60)
              GACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAA                                                                                                                                         >  SRR19973080.993613/1‑150 (MQ=60)
              GACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAA                                                                                                                                         >  SRR19973080.993647/1‑150 (MQ=60)
                CTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAA                                                                                                                                       >  SRR19973080.2413242/1‑150 (MQ=60)
                CTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAA                                                                                                                                       >  SRR19973080.2413228/1‑150 (MQ=60)
                  CGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATG                                                                                                                                     <  SRR19973080.389262/150‑1 (MQ=60)
                  CGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATG                                                                                                                                     <  SRR19973080.2666407/150‑1 (MQ=60)
                    TGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   >  SRR19973080.21938/1‑150 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973080.4378/150‑1 (MQ=60)
                    CGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGT                                                                                                                                   <  SRR19973080.4180/150‑1 (MQ=60)
                      CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCA                                                                                                                                 <  SRR19973080.3073151/150‑1 (MQ=60)
                      CCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCA                                                                                                                                 <  SRR19973080.4074877/150‑1 (MQ=60)
                       CTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAA                                                                                                                                >  SRR19973080.2922142/1‑150 (MQ=60)
                           CAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATC                                                                                                                            <  SRR19973080.2683882/150‑1 (MQ=60)
                           CAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATC                                                                                                                            >  SRR19973080.2446591/1‑150 (MQ=60)
                           CAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATC                                                                                                                            >  SRR19973080.2446456/1‑150 (MQ=60)
                            AGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCG                                                                                                                           >  SRR19973080.3224681/1‑150 (MQ=60)
                            AGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCG                                                                                                                           >  SRR19973080.3225528/1‑150 (MQ=60)
                               ATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACC                                                                                                                        <  SRR19973080.1822714/150‑1 (MQ=60)
                                TCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCT                                                                                                                       >  SRR19973080.3119445/1‑150 (MQ=60)
                                 CACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTG                                                                                                                      <  SRR19973080.3012286/150‑1 (MQ=60)
                                 CACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTG                                                                                                                      <  SRR19973080.3012259/150‑1 (MQ=60)
                                  ACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGA                                                                                                                     >  SRR19973080.1075703/1‑150 (MQ=60)
                                  ACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGA                                                                                                                     >  SRR19973080.1075580/1‑150 (MQ=60)
                                   CACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAA                                                                                                                    <  SRR19973080.1954588/150‑1 (MQ=60)
                                   CACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAA                                                                                                                    <  SRR19973080.1951708/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973080.1606006/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973080.3895863/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973080.4276685/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973080.4276613/150‑1 (MQ=60)
                                    ACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGATAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGTTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAAT                                                                                                                   <  SRR19973080.3892510/150‑1 (MQ=60)
                                       AAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGAC                                                                                                                <  SRR19973080.3771755/150‑1 (MQ=60)
                                       AAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGAC                                                                                                                <  SRR19973080.3773488/150‑1 (MQ=60)
                                       AAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGAC                                                                                                                <  SRR19973080.1139224/150‑1 (MQ=60)
                                         ACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAG                                                                                                              >  SRR19973080.1056779/1‑150 (MQ=60)
                                           TCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAA                                                                                                            <  SRR19973080.1735944/150‑1 (MQ=60)
                                             CTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAA                                                                                                          <  SRR19973080.4059442/150‑1 (MQ=60)
                                             CTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAA                                                                                                          >  SRR19973080.689835/1‑150 (MQ=60)
                                             CTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAA                                                                                                          >  SRR19973080.689676/1‑150 (MQ=60)
                                                     CGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTT                                                                                                  <  SRR19973080.3773880/150‑1 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               >  SRR19973080.3245816/1‑150 (MQ=60)
                                                        CCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGT                                                                                               >  SRR19973080.3247287/1‑150 (MQ=60)
                                                         CCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTG                                                                                              <  SRR19973080.1731436/150‑1 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             <  SRR19973080.612503/150‑1 (MQ=60)
                                                          CGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGC                                                                                             >  SRR19973080.1986946/1‑150 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          >  SRR19973080.3438527/1‑150 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          >  SRR19973080.3438106/1‑150 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          >  SRR19973080.220972/1‑150 (MQ=60)
                                                             CCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTC                                                                                          >  SRR19973080.220980/1‑150 (MQ=60)
                                                              CGGCAACGTCCTGCTCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTAT                                                                                         <  SRR19973080.944541/150‑1 (MQ=60)
                                                               GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTG                                                                                        >  SRR19973080.3901417/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973080.3108385/1‑150 (MQ=60)
                                                                  AACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACA                                                                                     >  SRR19973080.3108815/1‑150 (MQ=60)
                                                                      TCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAAT                                                                                 <  SRR19973080.3157589/150‑1 (MQ=60)
                                                                       CCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATT                                                                                <  SRR19973080.1408416/150‑1 (MQ=60)
                                                                          GCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAG                                                                             <  SRR19973080.2481937/150‑1 (MQ=60)
                                                                          GCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAG                                                                             <  SRR19973080.3886786/150‑1 (MQ=60)
                                                                             CACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGA                                                                          >  SRR19973080.1249576/1‑150 (MQ=60)
                                                                                   AGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAC                                                                    <  SRR19973080.2280064/150‑1 (MQ=60)
                                                                                   AGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAC                                                                    <  SRR19973080.2561983/150‑1 (MQ=60)
                                                                                   AGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAC                                                                    <  SRR19973080.2565099/150‑1 (MQ=60)
                                                                                    aataaccttttTTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACG                                                                   <  SRR19973080.2262526/139‑1 (MQ=60)
                                                                                        CTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCT                                                               >  SRR19973080.3130741/1‑150 (MQ=60)
                                                                                         TGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTT                                                              <  SRR19973080.1973546/150‑1 (MQ=60)
                                                                                           CTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTT                                                            <  SRR19973080.4327904/150‑1 (MQ=60)
                                                                                           CTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTT                                                            >  SRR19973080.175035/1‑150 (MQ=60)
                                                                                            TAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTT                                                           <  SRR19973080.4455513/150‑1 (MQ=60)
                                                                                                  ATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACA                                                     >  SRR19973080.3492526/1‑150 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGTTGACCAAAGCGGTAATTAATCGCTTTAATATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGAGGGGCTTTTTCGACCAGTGCGCCACGGTTA                                       <  SRR19973080.1044972/150‑1 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       >  SRR19973080.4324875/1‑150 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       <  SRR19973080.4442133/150‑1 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       <  SRR19973080.1045827/150‑1 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       <  SRR19973080.143578/150‑1 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       <  SRR19973080.144411/150‑1 (MQ=60)
                                                                                                                GAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTA                                       <  SRR19973080.4441749/150‑1 (MQ=60)
                                                                                                                   AGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATAT                                    >  SRR19973080.4251745/1‑150 (MQ=60)
                                                                                                                    GAACACGGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATA                                   >  SRR19973080.4481055/1‑150 (MQ=60)
                                                                                                                          GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             >  SRR19973080.3349024/1‑150 (MQ=60)
                                                                                                                          GGTCGCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCG                             >  SRR19973080.3348983/1‑150 (MQ=60)
                                                                                                                              GCCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAA                         >  SRR19973080.1787937/1‑150 (MQ=60)
                                                                                                                               CCCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAG                        >  SRR19973080.1648618/1‑150 (MQ=60)
                                                                                                                                CCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGG                       <  SRR19973080.52970/150‑1 (MQ=60)
                                                                                                                                CCGCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGG                       <  SRR19973080.53066/150‑1 (MQ=60)
                                                                                                                                  GCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTG                     <  SRR19973080.2026704/150‑1 (MQ=60)
                                                                                                                                  GCCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTG                     <  SRR19973080.4005838/150‑1 (MQ=60)
                                                                                                                                   CCTGATGACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGT                    <  SRR19973080.2797040/150‑1 (MQ=60)
                                                                                                                                     TGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCG                  >  SRR19973080.1120582/1‑150 (MQ=60)
                                                                                                                                       ATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTG                <  SRR19973080.3435630/150‑1 (MQ=60)
                                                                                                                                         GACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCC              <  SRR19973080.2698666/150‑1 (MQ=60)
                                                                                                                                         GACCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCC              <  SRR19973080.2698665/150‑1 (MQ=60)
                                                                                                                                           CCAAAGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCAC            >  SRR19973080.4450762/1‑150 (MQ=60)
                                                                                                                                            CAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACG           <  SRR19973080.4462166/150‑1 (MQ=60)
                                                                                                                                               AGCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCT        <  SRR19973080.2662244/150‑1 (MQ=60)
                                                                                                                                                GCGGTAATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTG       <  SRR19973080.142142/150‑1 (MQ=60)
                                                                                                                                                  GGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCC     <  SRR19973080.3277781/150‑1 (MQ=60)
                                                                                                                                                     AATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  <  SRR19973080.471265/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCAGCCTCCGTCAGACTCGCGCCTGGCAGAATCACACAAAACTCCTCACCACCGACCCGCCCGGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACT  >  CP009273/2020017‑2020315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑