Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,114,911 A→T 100% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,114,9110AT98.5% 953.5 / 2.4 274L42Q (CTG→CAG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (124/146);  total (125/149)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCC  >  CP009273/2114764‑2115057
                                                                                                                                                   |                                                                                                                                                  
cTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                  <  1:2751138/150‑1 (MQ=255)
cTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                  >  2:2901768/1‑150 (MQ=255)
 ttAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                                 >  1:4109379/1‑150 (MQ=255)
  tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTcc                                                                                                                                                <  1:692718/150‑1 (MQ=255)
    aGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGc                                                                                                                                              <  2:2431441/150‑1 (MQ=255)
    aGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGc                                                                                                                                              <  2:4027466/150‑1 (MQ=255)
     ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCt                                                                                                                                             <  2:801701/150‑1 (MQ=255)
      gTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTg                                                                                                                                            <  1:1421059/150‑1 (MQ=255)
      gTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTg                                                                                                                                            >  1:2871693/1‑150 (MQ=255)
        ccAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAc                                                                                                                                          <  2:1735673/150‑1 (MQ=255)
        ccAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAc                                                                                                                                          <  1:1557533/150‑1 (MQ=255)
               tGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaa                                                                                                                                   <  2:3912518/150‑1 (MQ=255)
                  aGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAcc                                                                                                                                <  1:4204849/150‑1 (MQ=255)
                   gCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCa                                                                                                                               >  1:187839/1‑150 (MQ=255)
                    cAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                              >  2:3464859/1‑150 (MQ=255)
                     aaCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGc                                                                                                                             >  2:467878/1‑150 (MQ=255)
                        aGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                          >  1:3817974/1‑150 (MQ=255)
                         gggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                         <  1:2171557/150‑1 (MQ=255)
                           gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGt                                                                                                                       <  1:932160/150‑1 (MQ=255)
                                  cTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaa                                                                                                                <  1:3449469/150‑1 (MQ=255)
                                   tGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                               >  1:2691085/1‑150 (MQ=255)
                                   tGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                               >  1:468330/1‑150 (MQ=255)
                                   tGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                               >  1:311296/1‑150 (MQ=255)
                                     tCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACa                                                                                                             >  2:3730423/1‑150 (MQ=255)
                                      cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                            >  2:3694277/1‑150 (MQ=255)
                                       gCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                           >  2:4530039/1‑150 (MQ=255)
                                          gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatgatg                                                                                                        <  2:3888865/150‑1 (MQ=255)
                                            ccGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGt                                                                                                      >  2:1820321/1‑150 (MQ=255)
                                            ccGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGt                                                                                                      >  1:1034506/1‑150 (MQ=255)
                                              gAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                    >  2:4141161/1‑150 (MQ=255)
                                                acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGata                                                                                                  <  1:3214478/150‑1 (MQ=255)
                                                 cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGatat                                                                                                 <  2:4335682/150‑1 (MQ=255)
                                                   ccGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCt                                                                                               <  1:3287322/150‑1 (MQ=255)
                                                         cATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAg                                                                                         <  1:3632041/150‑1 (MQ=255)
                                                         cATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAg                                                                                         >  1:1882101/1‑150 (MQ=255)
                                                           tGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCg                                                                                       <  1:2501456/150‑1 (MQ=255)
                                                            gAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGt                                                                                      <  2:2015873/150‑1 (MQ=255)
                                                               cGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGc                                                                                   <  2:3213700/150‑1 (MQ=255)
                                                                    aaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCAt                                                                              <  2:2955225/150‑1 (MQ=255)
                                                                     aaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATa                                                                             >  1:1091261/1‑150 (MQ=255)
                                                                      aaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATag                                                                            <  2:4063201/150‑1 (MQ=255)
                                                                       aTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATaga                                                                           <  1:3158808/150‑1 (MQ=255)
                                                                        tCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATagag                                                                          <  2:3096360/150‑1 (MQ=255)
                                                                          ggTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                        >  1:2361723/1‑150 (MQ=255)
                                                                          ggTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                        >  1:1546458/1‑150 (MQ=255)
                                                                           gTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAtt                                                                       <  2:1725921/150‑1 (MQ=255)
                                                                            tGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTa                                                                      <  2:1073486/150‑1 (MQ=255)
                                                                             gATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                     <  2:675807/150‑1 (MQ=255)
                                                                             gATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                     >  1:3414092/1‑150 (MQ=255)
                                                                              aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAc                                                                    >  1:162280/1‑150 (MQ=255)
                                                                               tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACg                                                                   <  1:2795359/150‑1 (MQ=255)
                                                                                  cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATTTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGTTTTCTGATAAGCGTTGCACCATATTGATTAACGATg                                                                <  1:1585977/150‑1 (MQ=255)
                                                                                  cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATg                                                                <  2:2544473/150‑1 (MQ=255)
                                                                                    cccAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                              >  1:3606560/1‑150 (MQ=255)
                                                                                     ccAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAt                                                             <  1:3730856/150‑1 (MQ=255)
                                                                                       aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTg                                                           >  2:269943/1‑150 (MQ=255)
                                                                                       aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTg                                                           >  2:4522361/1‑150 (MQ=255)
                                                                                       aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTg                                                           <  2:896994/150‑1 (MQ=255)
                                                                                        gCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTgg                                                          <  2:3538691/150‑1 (MQ=255)
                                                                                        gCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTgg                                                          <  2:3817974/150‑1 (MQ=255)
                                                                                         cATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                         <  1:3937870/150‑1 (MQ=255)
                                                                                         cATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                         >  1:4353991/1‑150 (MQ=255)
                                                                                         cATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATGGGt                                                         >  1:3890674/1‑150 (MQ=255)
                                                                                          aTCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtt                                                        >  2:350551/1‑150 (MQ=255)
                                                                                            cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                      <  2:1580110/150‑1 (MQ=255)
                                                                                            cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                      <  2:4169566/150‑1 (MQ=255)
                                                                                            cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                      >  1:53978/1‑150 (MQ=255)
                                                                                            cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                      >  1:4409780/1‑150 (MQ=255)
                                                                                                 acacCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctc                                                 >  1:1310851/1‑150 (MQ=255)
                                                                                                       ccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgctcgc                                           >  1:4033879/1‑150 (MQ=255)
                                                                                                           cGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                       <  2:2317144/150‑1 (MQ=255)
                                                                                                            gTAATCAGCGCCACCACCAGGTGCCTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGACATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCttt                                      <  1:1084911/150‑1 (MQ=255)
                                                                                                              aaTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCttttt                                    <  2:2135296/150‑1 (MQ=255)
                                                                                                               aTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttttt                                   <  1:2973121/150‑1 (MQ=255)
                                                                                                               aTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttttt                                   <  1:235091/150‑1 (MQ=255)
                                                                                                                tCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTa                                  <  2:3107224/150‑1 (MQ=255)
                                                                                                                  aGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGa                                >  2:1901174/1‑150 (MQ=255)
                                                                                                                   gcgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTAGCTCGCTCGCGCTTTTTTAGAt                               <  1:1048897/150‑1 (MQ=255)
                                                                                                                    cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAtt                              >  2:2786636/1‑150 (MQ=255)
                                                                                                                    cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAtt                              <  2:2171545/150‑1 (MQ=255)
                                                                                                                     gcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAttt                             >  2:1279035/1‑150 (MQ=255)
                                                                                                                        accaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTc                          <  2:2398521/150‑1 (MQ=255)
                                                                                                                          caACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTATATTTTTCAt                        >  1:860382/1‑150 (MQ=255)
                                                                                                                           aaCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATc                       >  1:1701841/1‑150 (MQ=255)
                                                                                                                             ctggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCTAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgt                     <  2:624152/148‑1 (MQ=255)
                                                                                                                               ggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                   <  2:806988/150‑1 (MQ=255)
                                                                                                                               ggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                   <  1:1980446/150‑1 (MQ=255)
                                                                                                                                   cATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTcc               <  2:3042057/150‑1 (MQ=255)
                                                                                                                                    aTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCt              <  1:2897678/150‑1 (MQ=255)
                                                                                                                                     tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg             <  1:3694261/150‑1 (MQ=255)
                                                                                                                                     tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg             <  2:4015323/150‑1 (MQ=255)
                                                                                                                                     tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg             <  2:3640089/150‑1 (MQ=255)
                                                                                                                                     tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg             <  1:3694363/150‑1 (MQ=255)
                                                                                                                                      gTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGTTATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGt            <  2:4199655/150‑1 (MQ=255)
                                                                                                                                      gTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTGTTTAGATTTGTCATCGTTGTTCCTGt            >  1:216545/1‑150 (MQ=255)
                                                                                                                                       tAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAAAATTGTCCTGCTTGTTCCTggt           >  2:4088290/1‑148 (MQ=255)
                                                                                                                                        agagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtta          >  2:3950027/1‑150 (MQ=255)
                                                                                                                                            gAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAg      <  1:1841255/150‑1 (MQ=255)
                                                                                                                                              aTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcc    >  2:4069511/1‑150 (MQ=255)
                                                                                                                                                gACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcccc  <  2:3347246/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCC  >  CP009273/2114764‑2115057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  CP009273/2114764‑2115060
                                                                                                                                                   |                                                                                                                                                     
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     >  SRR19973072.3839959/1‑150 (MQ=60)
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     <  SRR19973072.2757025/150‑1 (MQ=60)
 TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                                    >  SRR19973072.3431843/1‑150 (MQ=60)
 TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                                    >  SRR19973072.4118011/1‑150 (MQ=60)
 TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                                    >  SRR19973072.4117997/1‑150 (MQ=60)
  TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCC                                                                                                                                                   <  SRR19973072.694241/150‑1 (MQ=60)
  TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCC                                                                                                                                                   <  SRR19973072.1414373/150‑1 (MQ=60)
   AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                                  >  SRR19973072.808719/1‑150 (MQ=60)
    AGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGC                                                                                                                                                 <  SRR19973072.3187529/150‑1 (MQ=60)
      GTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTG                                                                                                                                               >  SRR19973072.2877847/1‑150 (MQ=60)
      GTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTG                                                                                                                                               <  SRR19973072.1424141/150‑1 (MQ=60)
        CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                             <  SRR19973072.1560845/150‑1 (MQ=60)
        ACAGTTTTGTAGCATCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGAGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTACGCTGAC                                                                                                                                             <  SRR19973072.1560870/150‑1 (MQ=60)
          AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                           <  SRR19973072.2016854/150‑1 (MQ=60)
          AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                           <  SRR19973072.1925214/150‑1 (MQ=60)
           GTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTC                                                                                                                                          <  SRR19973072.304570/150‑1 (MQ=60)
           GTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTC                                                                                                                                          <  SRR19973072.304619/150‑1 (MQ=60)
           GTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTC                                                                                                                                          <  SRR19973072.1326056/150‑1 (MQ=60)
            TTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCG                                                                                                                                         >  SRR19973072.1559296/1‑150 (MQ=60)
                  AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                                   <  SRR19973072.4213636/150‑1 (MQ=60)
                  AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                                   <  SRR19973072.4213637/150‑1 (MQ=60)
                   GCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCA                                                                                                                                  >  SRR19973072.188317/1‑150 (MQ=60)
                     AACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGC                                                                                                                                <  SRR19973072.4551674/150‑1 (MQ=60)
                        AGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAT                                                                                                                             >  SRR19973072.3825962/1‑150 (MQ=60)
                         GGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATA                                                                                                                            >  SRR19973072.4104811/1‑150 (MQ=60)
                         GGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATA                                                                                                                            <  SRR19973072.2176290/150‑1 (MQ=60)
                           GCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGT                                                                                                                          <  SRR19973072.934193/150‑1 (MQ=60)
                           GCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGT                                                                                                                          <  SRR19973072.934200/150‑1 (MQ=60)
                             AAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCC                                                                                                                        <  SRR19973072.2739570/150‑1 (MQ=60)
                             AAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCC                                                                                                                        <  SRR19973072.4181810/150‑1 (MQ=60)
                              AATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCG                                                                                                                       >  SRR19973072.3665291/1‑150 (MQ=60)
                               ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                                      >  SRR19973072.1048666/1‑150 (MQ=60)
                                 TCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCA                                                                                                                    >  SRR19973072.777877/1‑150 (MQ=60)
                                  CTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAA                                                                                                                   <  SRR19973072.3456704/150‑1 (MQ=60)
                                   TGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAA                                                                                                                  >  SRR19973072.2696812/1‑150 (MQ=60)
                                   TGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAA                                                                                                                  >  SRR19973072.469422/1‑150 (MQ=60)
                                   TGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAA                                                                                                                  >  SRR19973072.312011/1‑150 (MQ=60)
                                   TGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAA                                                                                                                  >  SRR19973072.3354340/1‑150 (MQ=60)
                                      CGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACAT                                                                                                               <  SRR19973072.1078936/150‑1 (MQ=60)
                                      CGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACAT                                                                                                               <  SRR19973072.1079568/150‑1 (MQ=60)
                                        CTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGA                                                                                                             <  SRR19973072.3805345/150‑1 (MQ=60)
                                         TGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGAT                                                                                                            >  SRR19973072.865301/1‑150 (MQ=60)
                                          GCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATG                                                                                                           >  SRR19973072.1729704/1‑150 (MQ=60)
                                            CCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGT                                                                                                         >  SRR19973072.2576879/1‑150 (MQ=60)
                                            CCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGT                                                                                                         >  SRR19973072.2734349/1‑150 (MQ=60)
                                            CCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGT                                                                                                         >  SRR19973072.1036743/1‑150 (MQ=60)
                                               AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      >  SRR19973072.2313437/1‑150 (MQ=60)
                                               AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      >  SRR19973072.3176221/1‑150 (MQ=60)
                                               AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      <  SRR19973072.1124100/150‑1 (MQ=60)
                                                ACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATA                                                                                                     <  SRR19973072.3221429/150‑1 (MQ=60)
                                                ACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATA                                                                                                     <  SRR19973072.3221277/150‑1 (MQ=60)
                                                 CACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATAT                                                                                                    <  SRR19973072.2048814/150‑1 (MQ=60)
                                                   CCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCT                                                                                                  <  SRR19973072.3294301/150‑1 (MQ=60)
                                                         CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                            <  SRR19973072.3639692/150‑1 (MQ=60)
                                                         CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                            >  SRR19973072.1883927/1‑150 (MQ=60)
                                                         CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                            >  SRR19973072.1884681/1‑150 (MQ=60)
                                                         CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                            >  SRR19973072.1886136/1‑150 (MQ=60)
                                                          ATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGC                                                                                           >  SRR19973072.3590649/1‑150 (MQ=60)
                                                           TGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCG                                                                                          <  SRR19973072.2506891/150‑1 (MQ=60)
                                                           TGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCG                                                                                          <  SRR19973072.4011631/150‑1 (MQ=60)
                                                              GCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTG                                                                                       >  SRR19973072.3791963/1‑150 (MQ=60)
                                                                     AAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATA                                                                                >  SRR19973072.1093569/1‑150 (MQ=60)
                                                                     AAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATA                                                                                >  SRR19973072.1093448/1‑150 (MQ=60)
                                                                      AATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAG                                                                               <  SRR19973072.239718/150‑1 (MQ=60)
                                                                      AATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAG                                                                               <  SRR19973072.239717/150‑1 (MQ=60)
                                                                       ATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGA                                                                              <  SRR19973072.3165323/150‑1 (MQ=60)
                                                                       ATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGA                                                                              <  SRR19973072.3165477/150‑1 (MQ=60)
                                                                          GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                           >  SRR19973072.1550256/1‑150 (MQ=60)
                                                                          GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                           >  SRR19973072.2366807/1‑150 (MQ=60)
                                                                          GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                           >  SRR19973072.1549731/1‑150 (MQ=60)
                                                                           GTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATT                                                                          >  SRR19973072.1825815/1‑150 (MQ=60)
                                                                            TGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTA                                                                         <  SRR19973072.4365672/150‑1 (MQ=60)
                                                                             GATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAA                                                                        >  SRR19973072.3421290/1‑150 (MQ=60)
                                                                              ATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAC                                                                       >  SRR19973072.162692/1‑150 (MQ=60)
                                                                              ATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAC                                                                       >  SRR19973072.4304837/1‑150 (MQ=60)
                                                                               TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                                      <  SRR19973072.2801232/150‑1 (MQ=60)
                                                                               TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                                      <  SRR19973072.2801295/150‑1 (MQ=60)
                                                                               TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                                      <  SRR19973072.2801340/150‑1 (MQ=60)
                                                                                  CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATTTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGTTTTCTGATAAGCGTTGCACCATATTGATTAACGATG                                                                   <  SRR19973072.1589381/150‑1 (MQ=60)
                                                                                  CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973072.2329512/150‑1 (MQ=60)
                                                                                  CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   >  SRR19973072.3623361/1‑150 (MQ=60)
                                                                                   GCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGC                                                                  >  SRR19973072.3233333/1‑150 (MQ=60)
                                                                                   GCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGC                                                                  >  SRR19973072.3935969/1‑150 (MQ=60)
                                                                                   GCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGC                                                                  >  SRR19973072.1602742/1‑150 (MQ=60)
                                                                                    CCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCA                                                                 <  SRR19973072.2816022/150‑1 (MQ=60)
                                                                                    CCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCA                                                                 >  SRR19973072.3614158/1‑150 (MQ=60)
                                                                                    CCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCA                                                                 >  SRR19973072.3614597/1‑150 (MQ=60)
                                                                                     CCATCATCTGGAACACCTCCAGCGTAATCAGCGACACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATATTCCGGCAAACATGATGGTGATATCTTAAAAGCGTTGCACCATAGAGATTAACGATGCAT                                                                <  SRR19973072.3738621/150‑1 (MQ=60)
                                                                                     CCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAT                                                                <  SRR19973072.3738708/150‑1 (MQ=60)
                                                                                         CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGT                                                            >  SRR19973072.4363817/1‑150 (MQ=60)
                                                                                         CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGT                                                            <  SRR19973072.3946094/150‑1 (MQ=60)
                                                                                         CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGT                                                            >  SRR19973072.4363122/1‑150 (MQ=60)
                                                                                         CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATGGGT                                                            >  SRR19973072.3898771/1‑150 (MQ=60)
                                                                                            CTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTT                                                         >  SRR19973072.4419059/1‑150 (MQ=60)
                                                                                            CTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTT                                                         >  SRR19973072.54116/1‑150 (MQ=60)
                                                                                            CTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTT                                                         <  SRR19973072.163758/150‑1 (MQ=60)
                                                                                               GAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGC                                                      >  SRR19973072.634143/1‑150 (MQ=60)
                                                                                                 ACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTC                                                    <  SRR19973072.150512/150‑1 (MQ=60)
                                                                                                 ACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTC                                                    >  SRR19973072.1313703/1‑150 (MQ=60)
                                                                                                    CCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCT                                                 <  SRR19973072.3727127/150‑1 (MQ=60)
                                                                                                       CCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGC                                              >  SRR19973072.4042352/1‑150 (MQ=60)
                                                                                                            GTAATCAGCGCCACCACCAGGTGCCTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGACATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                         <  SRR19973072.1087211/150‑1 (MQ=60)
                                                                                                            GTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                         <  SRR19973072.1087268/150‑1 (MQ=60)
                                                                                                              AATCAGCGCCTCCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTT                                       <  SRR19973072.1455277/150‑1 (MQ=60)
                                                                                                               ATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTT                                      <  SRR19973072.1863221/150‑1 (MQ=60)
                                                                                                               ATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTT                                      <  SRR19973072.2979463/150‑1 (MQ=60)
                                                                                                               ATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTT                                      <  SRR19973072.235705/150‑1 (MQ=60)
                                                                                                               ATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTT                                      <  SRR19973072.235683/150‑1 (MQ=60)
                                                                                                               ATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTT                                      <  SRR19973072.235671/150‑1 (MQ=60)
                                                                                                                   GCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTAGCTCGCTCGCGCTTTTTTAGAT                                  <  SRR19973072.1051152/150‑1 (MQ=60)
                                                                                                                     GCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTT                                <  SRR19973072.3668421/150‑1 (MQ=60)
                                                                                                                        ACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTC                             >  SRR19973072.3592552/1‑150 (MQ=60)
                                                                                                                          CAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTATATTTTTCAT                           >  SRR19973072.862235/1‑150 (MQ=60)
                                                                                                                          CAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAT                           >  SRR19973072.3835372/1‑150 (MQ=60)
                                                                                                                           AACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATTCATTGTTTTTCGCTCGCTCGCGCTTTTTTAtagttgtaata                          >  SRR19973072.1705556/1‑139 (MQ=60)
                                                                                                                           AACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATC                          >  SRR19973072.1705573/1‑150 (MQ=60)
                                                                                                                               GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTG                      <  SRR19973072.1984694/150‑1 (MQ=60)
                                                                                                                               GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTG                      <  SRR19973072.1984673/150‑1 (MQ=60)
                                                                                                                                    ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT                 <  SRR19973072.2903931/150‑1 (MQ=60)
                                                                                                                                    ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT                 <  SRR19973072.2903920/150‑1 (MQ=60)
                                                                                                                                    ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT                 <  SRR19973072.2903882/150‑1 (MQ=60)
                                                                                                                                    ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT                 <  SRR19973072.2903874/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG                <  SRR19973072.3702151/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG                <  SRR19973072.3702049/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG                <  SRR19973072.3702065/150‑1 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGT               >  SRR19973072.217357/1‑150 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGT               <  SRR19973072.1593161/150‑1 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGT               <  SRR19973072.3971222/150‑1 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGT               <  SRR19973072.3304638/150‑1 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTACTGT               >  SRR19973072.2802297/1‑150 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTGTTTAGATTTGTCATCGTTGTTCCTGT               >  SRR19973072.217089/1‑150 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCGCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGT               >  SRR19973072.479318/1‑150 (MQ=60)
                                                                                                                                      GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCGCGCGCGCGCTTTTTTAGATTTTTCATAGTTGTTCCTGT               >  SRR19973072.4464508/1‑150 (MQ=60)
                                                                                                                                       TAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTT              >  SRR19973072.90260/1‑150 (MQ=60)
                                                                                                                                       TAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGAGATATCTGAAAAGCGTTGCACCATATAGATTAACGATGCATTGGGTTTCGCTCGCTCGCGCTTTTTTAGAGTTGTCATCGTTTTTCATGTT              >  SRR19973072.1933026/1‑150 (MQ=60)
                                                                                                                                        AGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGGTCCTGTTA             >  SRR19973072.5071/1‑150 (MQ=60)
                                                                                                                                            GAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAG         <  SRR19973072.1845252/150‑1 (MQ=60)
                                                                                                                                                   TGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCGGTTATTAGCCCCTTA  >  SRR19973072.3240765/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  CP009273/2114764‑2115060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑