Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,114,911 A→T 100% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,114,9110AT100.0% 734.4 / NA 221L42Q (CTG→CAG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (89/132);  total (89/132)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCC  >  CP009273/2114766‑2115055
                                                                                                                                                 |                                                                                                                                                
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                >  1:750309/1‑148 (MQ=255)
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                <  1:3298336/148‑1 (MQ=255)
 aaGGTCCAGTTTTTTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATGTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                               <  2:3065015/148‑1 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                             <  2:3503418/148‑1 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                             >  1:1280405/1‑148 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                             >  1:638947/1‑148 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                             <  2:2383200/148‑1 (MQ=255)
      ccAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTg                                                                                                                                          <  1:1116315/148‑1 (MQ=255)
          ttttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACtt                                                                                                                                      >  1:3682866/1‑148 (MQ=255)
            ttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCg                                                                                                                                    <  2:414651/148‑1 (MQ=255)
                aGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaaa                                                                                                                                <  1:2680529/148‑1 (MQ=255)
                aGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaaa                                                                                                                                >  2:2491052/1‑148 (MQ=255)
                    aCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                            <  2:3692526/148‑1 (MQ=255)
                    aCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                            <  1:214753/148‑1 (MQ=255)
                       gggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCa                                                                                                                         <  2:2537348/148‑1 (MQ=255)
                        ggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                        >  2:1283669/1‑148 (MQ=255)
                        ggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                        >  1:943523/1‑148 (MQ=255)
                        ggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                        >  2:3535471/1‑148 (MQ=255)
                          cAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAg                                                                                                                      <  2:2651435/148‑1 (MQ=255)
                            aaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTc                                                                                                                    >  2:1725716/1‑148 (MQ=255)
                               tCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCgg                                                                                                                 <  1:535044/148‑1 (MQ=255)
                               tCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCgg                                                                                                                 >  1:1761527/1‑148 (MQ=255)
                               tCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCgg                                                                                                                 <  2:2688822/148‑1 (MQ=255)
                                    cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATACCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAAc                                                                                                            <  1:1146843/148‑1 (MQ=255)
                                       tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCACCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                         <  2:2558888/148‑1 (MQ=255)
                                       tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                         <  2:2153636/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                        <  2:675168/148‑1 (MQ=255)
                                         cccGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatgat                                                                                                       >  2:1575716/1‑148 (MQ=255)
                                          ccGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatgatg                                                                                                      <  2:331294/148‑1 (MQ=255)
                                             aaCACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTg                                                                                                   <  1:1128087/148‑1 (MQ=255)
                                              acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                  >  1:3412414/1‑148 (MQ=255)
                                               cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGat                                                                                                 <  2:3280348/148‑1 (MQ=255)
                                               cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGat                                                                                                 >  1:918187/1‑148 (MQ=255)
                                               cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGat                                                                                                 <  2:489106/148‑1 (MQ=255)
                                                 ccGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGatat                                                                                               >  1:1634328/1‑148 (MQ=255)
                                                 ccGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGatat                                                                                               >  1:1634294/1‑148 (MQ=255)
                                                       cATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGaaa                                                                                         <  2:2361804/148‑1 (MQ=255)
                                                         tGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAg                                                                                       >  1:3380190/1‑148 (MQ=255)
                                                              gATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTg                                                                                  <  2:3655820/148‑1 (MQ=255)
                                                               aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGc                                                                                 >  1:1978707/1‑148 (MQ=255)
                                                                tAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCa                                                                                <  1:2970770/148‑1 (MQ=255)
                                                                tAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCa                                                                                <  2:2921912/148‑1 (MQ=255)
                                                                     aTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATa                                                                           >  2:1443672/1‑148 (MQ=255)
                                                                         gTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAgaga                                                                       <  1:2420030/148‑1 (MQ=255)
                                                                          tGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                      <  1:1021433/148‑1 (MQ=255)
                                                                          tGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                      >  1:33098/1‑148 (MQ=255)
                                                                             tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                   <  1:977893/148‑1 (MQ=255)
                                                                             tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                   <  1:260817/148‑1 (MQ=255)
                                                                             tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                   <  2:2882313/148‑1 (MQ=255)
                                                                             tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                   <  1:2473520/148‑1 (MQ=255)
                                                                               ccgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACg                                                                 <  2:631013/148‑1 (MQ=255)
                                                                                cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGa                                                                <  1:2817831/148‑1 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            >  2:442555/1‑148 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            <  2:1868103/148‑1 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            >  2:2221550/1‑148 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            <  1:3166520/148‑1 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            >  2:3257350/1‑148 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            >  2:1221920/1‑148 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            <  2:3412414/148‑1 (MQ=255)
                                                                                    cAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCa                                                            <  2:3757028/148‑1 (MQ=255)
                                                                                       cATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTg                                                         <  1:681041/148‑1 (MQ=255)
                                                                                         tCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                       <  2:3158285/148‑1 (MQ=255)
                                                                                         tCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                       <  2:1901809/148‑1 (MQ=255)
                                                                                         tCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                       >  1:3753101/1‑148 (MQ=255)
                                                                                          cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtt                                                      <  1:3618882/148‑1 (MQ=255)
                                                                                            ggAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                    <  2:1616835/148‑1 (MQ=255)
                                                                                              aaCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                  <  2:1953277/148‑1 (MQ=255)
                                                                                              aaCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                  <  1:1323274/148‑1 (MQ=255)
                                                                                               acacCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgc                                                 >  2:1091363/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                                >  1:2562905/1‑148 (MQ=255)
                                                                                                  ccaccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcg                                              <  2:1945712/148‑1 (MQ=255)
                                                                                                  ccaccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATGGGTTTtcgctcg                                              >  1:1360365/1‑148 (MQ=255)
                                                                                                          gTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCt                                      <  2:750309/148‑1 (MQ=255)
                                                                                                          gTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCt                                      <  1:1198547/148‑1 (MQ=255)
                                                                                                           tAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                     <  2:1354883/148‑1 (MQ=255)
                                                                                                           tAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                     >  2:825227/1‑148 (MQ=255)
                                                                                                                  cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGa                              <  2:638947/148‑1 (MQ=255)
                                                                                                                   gcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAt                             <  2:853369/148‑1 (MQ=255)
                                                                                                                   gcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAt                             <  1:2491052/148‑1 (MQ=255)
                                                                                                                     caccaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAttt                           >  1:3392196/1‑148 (MQ=255)
                                                                                                                      accaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTg                          <  1:2232369/148‑1 (MQ=255)
                                                                                                                        caACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTc                        <  2:560660/148‑1 (MQ=255)
                                                                                                                        caACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTc                        <  1:869459/148‑1 (MQ=255)
                                                                                                                        caACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTc                        <  1:527749/148‑1 (MQ=255)
                                                                                                                             ggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgt                   <  2:1777671/148‑1 (MQ=255)
                                                                                                                             ggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgt                   <  2:3669262/148‑1 (MQ=255)
                                                                                                                              gTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgtt                  <  1:1892022/148‑1 (MQ=255)
                                                                                                                              gTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgtt                  >  1:1251245/1‑148 (MQ=255)
                                                                                                                               tGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                 <  1:1598455/148‑1 (MQ=255)
                                                                                                                               tGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                 <  1:442384/148‑1 (MQ=255)
                                                                                                                                   tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTcc             >  1:1060968/1‑148 (MQ=255)
                                                                                                                                    gTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCt            <  2:2374046/148‑1 (MQ=255)
                                                                                                                                     tAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg           <  2:3184412/148‑1 (MQ=255)
                                                                                                                                     tAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg           <  2:1852685/148‑1 (MQ=255)
                                                                                                                                      agagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGt          <  2:1018710/148‑1 (MQ=255)
                                                                                                                                          gAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatt      >  1:583297/1‑148 (MQ=255)
                                                                                                                                          gAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCGCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatt      >  1:3681504/1‑148 (MQ=255)
                                                                                                                                           aaTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatta     >  1:2326818/1‑148 (MQ=255)
                                                                                                                                           aaTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCGCGCTCGCGCCTTTTTAGATTTGTCATCGGTGTTGCTGttatta     >  1:1452194/1‑148 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc   >  2:3197862/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCC  >  CP009273/2114766‑2115055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTA  >  CP009273/2114762‑2115052
                                                                                                                                                     |                                                                                                                                             
CGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACT                                                                                                                                               <  SRR19973069.2072486/150‑1 (MQ=60)
  CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                             >  SRR19973069.755619/1‑150 (MQ=60)
   TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                            <  SRR19973069.1226429/150‑1 (MQ=60)
   TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                            <  SRR19973069.1898911/150‑1 (MQ=60)
   TTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTC                                                                                                                                            <  SRR19973069.1899144/150‑1 (MQ=60)
    TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCC                                                                                                                                           <  SRR19973069.3319936/150‑1 (MQ=60)
     AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                          >  SRR19973069.1288983/1‑150 (MQ=60)
     AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                          >  SRR19973069.643149/1‑150 (MQ=60)
          CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                     <  SRR19973069.1124197/150‑1 (MQ=60)
          CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                     <  SRR19973069.1124104/150‑1 (MQ=60)
            AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                   >  SRR19973069.3706953/1‑150 (MQ=60)
            AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                   >  SRR19973069.3705072/1‑150 (MQ=60)
                   TAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAC                                                                                                                            <  SRR19973069.391529/150‑1 (MQ=60)
                    AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                           <  SRR19973069.492223/150‑1 (MQ=60)
                    AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                           <  SRR19973069.2698502/150‑1 (MQ=60)
                    AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                           >  SRR19973069.3466467/1‑150 (MQ=60)
                      CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                         >  SRR19973069.1865620/1‑150 (MQ=60)
                        ACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCC                                                                                                                       <  SRR19973069.216432/150‑1 (MQ=60)
                        ACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCC                                                                                                                       <  SRR19973069.216438/150‑1 (MQ=60)
                         CAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCA                                                                                                                      >  SRR19973069.2387293/1‑150 (MQ=60)
                          AGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAT                                                                                                                     >  SRR19973069.949904/1‑150 (MQ=60)
                          AGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAT                                                                                                                     >  SRR19973069.949885/1‑150 (MQ=60)
                                 ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                              >  SRR19973069.684272/1‑150 (MQ=60)
                                 ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                              >  SRR19973069.1773963/1‑150 (MQ=60)
                                 ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                              >  SRR19973069.1773949/1‑150 (MQ=60)
                                 ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                              >  SRR19973069.3248985/1‑150 (MQ=60)
                                   TCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCA                                                                                                            <  SRR19973069.538629/150‑1 (MQ=60)
                                   TCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCA                                                                                                            <  SRR19973069.538817/150‑1 (MQ=60)
                                        CGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATACCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACAT                                                                                                       <  SRR19973069.1154848/150‑1 (MQ=60)
                                           TGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGAT                                                                                                    <  SRR19973069.1655265/150‑1 (MQ=60)
                                             CCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGG                                                                                                  <  SRR19973069.1639651/150‑1 (MQ=60)
                                              CCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGT                                                                                                 >  SRR19973069.1364285/1‑150 (MQ=60)
                                                GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                               >  SRR19973069.3434652/1‑150 (MQ=60)
                                                 AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                              >  SRR19973069.924451/1‑150 (MQ=60)
                                                 AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                              <  SRR19973069.1135950/150‑1 (MQ=60)
                                                   CACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATAT                                                                                            >  SRR19973069.1645503/1‑150 (MQ=60)
                                                   CACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATAT                                                                                            >  SRR19973069.1645478/1‑150 (MQ=60)
                                                   CACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATAT                                                                                            >  SRR19973069.1645512/1‑150 (MQ=60)
                                                           CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                    >  SRR19973069.3401581/1‑150 (MQ=60)
                                                           CATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAG                                                                                    >  SRR19973069.3402271/1‑150 (MQ=60)
                                                            ATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGC                                                                                   <  SRR19973069.3139971/150‑1 (MQ=60)
                                                                 CGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGC                                                                              >  SRR19973069.1992467/1‑150 (MQ=60)
                                                                 CGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGC                                                                              >  SRR19973069.1992470/1‑150 (MQ=60)
                                                                 CGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGC                                                                              >  SRR19973069.2493405/1‑150 (MQ=60)
                                                                   cagcacgtcgccgatgagatcactcaacaaaAACACCACCAGCGTAATCAGCGCCCCCAACAGGTGCATGTAGACGAATGACTGTCCGCTGACTTCGCAAACCAGCCctaagatcggaagaccacacgtctcaactcccgtcacatcctc                                                                            >  SRR19973069.3363107/32‑107 (MQ=60)
                                                                    TAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACC                                                                           <  SRR19973069.2990493/150‑1 (MQ=60)
                                                                        AATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAG                                                                       <  SRR19973069.1199829/150‑1 (MQ=60)
                                                                            GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                   <  SRR19973069.3217929/150‑1 (MQ=60)
                                                                            GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                   >  SRR19973069.876738/1‑150 (MQ=60)
                                                                            GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                   >  SRR19973069.33566/1‑150 (MQ=60)
                                                                            GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                   >  SRR19973069.33547/1‑150 (MQ=60)
                                                                             GTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATT                                                                  <  SRR19973069.2436375/150‑1 (MQ=60)
                                                                              TGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTA                                                                 <  SRR19973069.2836244/150‑1 (MQ=60)
                                                                              TGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTA                                                                 <  SRR19973069.1028502/150‑1 (MQ=60)
                                                                              TGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTA                                                                 <  SRR19973069.927246/150‑1 (MQ=60)
                                                                                ATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAC                                                               <  SRR19973069.2594134/150‑1 (MQ=60)
                                                                                 TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                              <  SRR19973069.983784/150‑1 (MQ=60)
                                                                                 TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                              <  SRR19973069.2490108/150‑1 (MQ=60)
                                                                                 TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                              <  SRR19973069.262696/150‑1 (MQ=60)
                                                                                 TGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACG                                                              <  SRR19973069.984479/150‑1 (MQ=60)
                                                                                   CCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGAT                                                            <  SRR19973069.1217311/150‑1 (MQ=60)
                                                                                    CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                           <  SRR19973069.2836484/150‑1 (MQ=60)
                                                                                      CCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCA                                                         >  SRR19973069.3721031/1‑150 (MQ=60)
                                                                                        CAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATT                                                       <  SRR19973069.3187158/150‑1 (MQ=60)
                                                                                           CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGT                                                    <  SRR19973069.685775/150‑1 (MQ=60)
                                                                                           CATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGT                                                    >  SRR19973069.3777524/1‑150 (MQ=60)
                                                                                            ATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTT                                                   >  SRR19973069.1320221/1‑150 (MQ=60)
                                                                                              CTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTT                                                 <  SRR19973069.3642523/150‑1 (MQ=60)
                                                                                               AGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTC                                                >  SRR19973069.1111786/1‑150 (MQ=60)
                                                                                                  AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCtcg                                             >  SRR19973069.2579828/1‑147 (MQ=60)
                                                                                                  AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCT                                             <  SRR19973069.1332369/150‑1 (MQ=60)
                                                                                                    CACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATGGGTTTTCGCTCG                                           >  SRR19973069.1369819/1‑150 (MQ=60)
                                                                                                       CACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTC                                        >  SRR19973069.2407317/1‑150 (MQ=60)
                                                                                                            GCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCT                                   <  SRR19973069.2323456/150‑1 (MQ=60)
                                                                                                              GTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                 <  SRR19973069.1206789/150‑1 (MQ=60)
                                                                                                              GTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                 >  SRR19973069.1439781/1‑150 (MQ=60)
                                                                                                              GTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                 >  SRR19973069.2470081/1‑150 (MQ=60)
                                                                                                               TAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTT                                <  SRR19973069.3571168/150‑1 (MQ=60)
                                                                                                                AATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTT                               <  SRR19973069.1278331/150‑1 (MQ=60)
                                                                                                                   CAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAG                            <  SRR19973069.579738/150‑1 (MQ=60)
                                                                                                                    AGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGA                           <  SRR19973069.829257/150‑1 (MQ=60)
                                                                                                                      CGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATT                         <  SRR19973069.1381322/150‑1 (MQ=60)
                                                                                                                       GCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTT                        >  SRR19973069.3414348/1‑150 (MQ=60)
                                                                                                                       GCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTT                        >  SRR19973069.3414260/1‑150 (MQ=60)
                                                                                                                       GCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTT                        <  SRR19973069.2507715/150‑1 (MQ=60)
                                                                                                                          ACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTC                     <  SRR19973069.2247443/150‑1 (MQ=60)
                                                                                                                            CAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAT                   <  SRR19973069.531474/150‑1 (MQ=60)
                                                                                                                            CAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAT                   <  SRR19973069.875515/150‑1 (MQ=60)
                                                                                                                             AACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATC                  <  SRR19973069.641139/150‑1 (MQ=60)
                                                                                                                                AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATAGTT               >  SRR19973069.1259717/1‑150 (MQ=60)
                                                                                                                                  GTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGT             <  SRR19973069.1905141/150‑1 (MQ=60)
                                                                                                                                   TGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTT            <  SRR19973069.1609237/150‑1 (MQ=60)
                                                                                                                                   TGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTT            <  SRR19973069.1172266/150‑1 (MQ=60)
                                                                                                                                   TGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTT            <  SRR19973069.445619/150‑1 (MQ=60)
                                                                                                                                   TGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTT            <  SRR19973069.445791/150‑1 (MQ=60)
                                                                                                                                   TGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTT            <  SRR19973069.2236578/150‑1 (MQ=60)
                                                                                                                                     CATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCC          >  SRR19973069.1068316/1‑150 (MQ=60)
                                                                                                                                     CATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCC          >  SRR19973069.1068353/1‑150 (MQ=60)
                                                                                                                                      ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATCGAGATTAACGATGCAGTGGTTTTCGCTCGCTCGCGCGTTGTTAGATTTGTCATCGGTGTTCCT         >  SRR19973069.2605720/1‑150 (MQ=60)
                                                                                                                                      ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT         >  SRR19973069.684803/1‑150 (MQ=60)
                                                                                                                                         TAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTT      <  SRR19973069.1153877/150‑1 (MQ=60)
                                                                                                                                          AGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCGTGTTA     >  SRR19973069.2916175/1‑150 (MQ=60)
                                                                                                                                           GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT    <  SRR19973069.695889/150‑1 (MQ=60)
                                                                                                                                            AGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTGATCGTTGTTGCTGTTATT   >  SRR19973069.3705871/1‑150 (MQ=60)
                                                                                                                                            AGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATT   >  SRR19973069.587291/1‑150 (MQ=60)
                                                                                                                                            AGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATT   <  SRR19973069.2627725/150‑1 (MQ=60)
                                                                                                                                            AGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATT   >  SRR19973069.2518004/1‑150 (MQ=60)
                                                                                                                                            AGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCGCGCGCTTTTTTAGATTTGTCATCGTTGTTGCTGTTATT   >  SRR19973069.3705583/1‑150 (MQ=60)
                                                                                                                                             GGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTA  >  SRR19973069.3430824/1‑150 (MQ=60)
                                                                                                                                             GGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTA  >  SRR19973069.2342641/1‑150 (MQ=60)
                                                                                                                                             GGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTA  >  SRR19973069.1518816/1‑150 (MQ=60)
                                                                                                                                             GGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCGCGCTCGCGCCTTTTTAGATTTGTCATCGGTGTTGCTGTTATTA  >  SRR19973069.1462333/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                             
CGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTA  >  CP009273/2114762‑2115052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑