Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,114,911 A→T 100% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,114,9110AT100.0% 472.6 / NA 142L42Q (CTG→CAG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (56/86);  total (56/86)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 142 total aligned reads displayed.

TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGC  >  CP009273/2114766‑2115054
                                                                                                                                                 |                                                                                                                                               
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                               <  1:3133256/148‑1 (MQ=255)
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                               <  1:1232899/148‑1 (MQ=255)
 aaGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                              <  2:42189/148‑1 (MQ=255)
 aaGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                              <  2:614837/148‑1 (MQ=255)
 aaGGTCCAGTTTTGTAGCAACAGGGCAAAGTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                              <  2:614811/148‑1 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGNTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                            >  1:1680098/1‑148 (MQ=255)
   ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCg                                                                                                                                            >  1:2763315/1‑148 (MQ=255)
    gTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGc                                                                                                                                           >  1:2582354/1‑148 (MQ=255)
     tCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCt                                                                                                                                          <  1:926101/148‑1 (MQ=255)
     tCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCt                                                                                                                                          <  1:926099/148‑1 (MQ=255)
        aGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAc                                                                                                                                       <  1:3212231/148‑1 (MQ=255)
           tttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTc                                                                                                                                    <  2:824278/148‑1 (MQ=255)
               tAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaa                                                                                                                                >  2:921268/1‑148 (MQ=255)
               tAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaa                                                                                                                                >  1:100306/1‑148 (MQ=255)
                  cAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAcc                                                                                                                             <  2:2956133/148‑1 (MQ=255)
                    aCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                           >  2:1380227/1‑148 (MQ=255)
                    aCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                           >  2:2701794/1‑148 (MQ=255)
                     cAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGc                                                                                                                          >  2:428028/1‑148 (MQ=255)
                       gggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCa                                                                                                                        <  2:2215243/148‑1 (MQ=255)
                       gggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCa                                                                                                                        >  1:624637/1‑148 (MQ=255)
                         gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCTACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                      <  2:2408629/148‑1 (MQ=255)
                            aaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTc                                                                                                                   <  2:922682/148‑1 (MQ=255)
                            aaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTc                                                                                                                   <  1:2893619/148‑1 (MQ=255)
                             aTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTcc                                                                                                                  >  1:2636257/1‑148 (MQ=255)
                                  gTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaa                                                                                                             >  1:2666800/1‑148 (MQ=255)
                                   tCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                            <  2:14392/148‑1 (MQ=255)
                                   tCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                            >  1:993484/1‑148 (MQ=255)
                                       tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                        <  1:3265291/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                       <  2:2069996/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                       <  1:322497/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                       <  2:2247192/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                       <  2:2247416/148‑1 (MQ=255)
                                            gAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCACCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGt                                                                                                   <  1:121521/148‑1 (MQ=255)
                                              acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                 >  2:2869086/1‑148 (MQ=255)
                                              acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                 >  1:1709823/1‑148 (MQ=255)
                                              acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                 >  1:495962/1‑148 (MQ=255)
                                                         tGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAg                                                                                      <  1:975161/148‑1 (MQ=255)
                                                           aGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCg                                                                                    <  1:1178175/148‑1 (MQ=255)
                                                            gCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGt                                                                                   <  2:2695962/148‑1 (MQ=255)
                                                            gCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGt                                                                                   <  2:879003/148‑1 (MQ=255)
                                                            gCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGt                                                                                   <  2:879009/148‑1 (MQ=255)
                                                               aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGc                                                                                >  2:482869/1‑148 (MQ=255)
                                                               aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGc                                                                                >  1:1358414/1‑148 (MQ=255)
                                                                tAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCa                                                                               >  2:1496734/1‑148 (MQ=255)
                                                                 aaaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                              >  2:2621050/1‑148 (MQ=255)
                                                                 aaaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                              <  2:1097650/148‑1 (MQ=255)
                                                                 aaaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGATGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                              >  2:2930763/1‑148 (MQ=255)
                                                                      tCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATag                                                                         >  2:1448183/1‑148 (MQ=255)
                                                                      tCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATag                                                                         >  2:1448224/1‑148 (MQ=255)
                                                                           gATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAtt                                                                    >  2:125659/1‑148 (MQ=255)
                                                                            aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTa                                                                   >  1:1687686/1‑148 (MQ=255)
                                                                            aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTa                                                                   >  2:2042595/1‑148 (MQ=255)
                                                                            aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTa                                                                   <  2:2022109/148‑1 (MQ=255)
                                                                                 gccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGAt                                                              <  2:1848794/148‑1 (MQ=255)
                                                                                     aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAt                                                          <  1:2296902/148‑1 (MQ=255)
                                                                                      gCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAtt                                                         <  1:2575782/148‑1 (MQ=255)
                                                                                        aTCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTgg                                                       <  1:1006702/148‑1 (MQ=255)
                                                                                         tCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGt                                                      >  2:2437616/1‑148 (MQ=255)
                                                                                            ggAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                   <  1:2217024/148‑1 (MQ=255)
                                                                                            ggAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                   >  2:3078364/1‑148 (MQ=255)
                                                                                             gAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtc                                                  >  2:3067125/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                               >  1:374994/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                               <  2:278458/148‑1 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                               >  2:1247952/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                               <  2:1709823/148‑1 (MQ=255)
                                                                                                 accaccAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctc                                              >  1:1149433/1‑148 (MQ=255)
                                                                                                  ccaccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcg                                             <  1:2986807/148‑1 (MQ=255)
                                                                                                  ccaccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcg                                             <  1:2815546/148‑1 (MQ=255)
                                                                                                    accaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgct                                           <  2:3081219/148‑1 (MQ=255)
                                                                                                     ccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgctc                                          >  2:2519395/1‑148 (MQ=255)
                                                                                                     ccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgctc                                          >  2:2717325/1‑148 (MQ=255)
                                                                                                      caGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGACTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTtcgctcgctcg                                         >  1:2313105/1‑148 (MQ=255)
                                                                                                      caGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtggctcgctcg                                         >  1:2037533/1‑148 (MQ=255)
                                                                                                      caGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgctcg                                         <  1:2779317/148‑1 (MQ=255)
                                                                                                        gCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTcgcg                                       <  2:1680098/148‑1 (MQ=255)
                                                                                                               cAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGCTGCATTGGTTTTCGCTCGCTCGCGCtttttt                                >  2:1854016/1‑148 (MQ=255)
                                                                                                               cAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttttt                                >  2:861112/1‑148 (MQ=255)
                                                                                                               cAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttttt                                <  1:1496734/148‑1 (MQ=255)
                                                                                                                  cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGa                             <  2:1631474/148‑1 (MQ=255)
                                                                                                                     caccaACAGGTGCATGTAGAGGTATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAttt                          <  2:512349/148‑1 (MQ=255)
                                                                                                                     caccaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGTTATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAttt                          <  2:512823/148‑1 (MQ=255)
                                                                                                                      accaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTg                         <  2:1293535/148‑1 (MQ=255)
                                                                                                                         aaCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCa                      >  1:1321972/1‑148 (MQ=255)
                                                                                                                            aGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGATAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCg                   <  2:833381/148‑1 (MQ=255)
                                                                                                                            aGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCg                   <  1:2964585/148‑1 (MQ=255)
                                                                                                                            aGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCg                   <  1:2964672/148‑1 (MQ=255)
                                                                                                                              gTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgtt                 >  1:580793/1‑148 (MQ=255)
                                                                                                                               tGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCAGAGAGATTAACGATGCATGGGTTTTCGCTCGCGCGCGCTGGTTTAGATTTGTGATCggtg                >  1:995964/1‑148 (MQ=255)
                                                                                                                                  aTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTc             <  2:1195124/148‑1 (MQ=255)
                                                                                                                                   tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTcc            <  1:3119466/148‑1 (MQ=255)
                                                                                                                                   tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTcc            <  1:1025705/148‑1 (MQ=255)
                                                                                                                                   tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAGAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTcc            <  1:1477645/148‑1 (MQ=255)
                                                                                                                                       gagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtt        <  1:2621050/148‑1 (MQ=255)
                                                                                                                                         ggAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttat      <  2:1599382/148‑1 (MQ=255)
                                                                                                                                          gAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatt     <  1:1232668/148‑1 (MQ=255)
                                                                                                                                           taTGACTGTCCGCTGACTTCGCTAACCAGCCATAGTCCGGCAAACATGATGGTTATATCTGAAAAGCTTTGCACCATTGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatta    <  2:350563/147‑1 (MQ=255)
                                                                                                                                           aaTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCCTAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatta    <  2:3202249/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc  <  1:195734/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc  <  1:196017/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc  <  1:224076/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGC  >  CP009273/2114766‑2115054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC  >  CP009273/2114764‑2115056
                                                                                                                                                   |                                                                                                                                                 
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                 <  SRR19973070.222629/150‑1 (MQ=60)
  TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCC                                                                                                                                               <  SRR19973070.3153494/150‑1 (MQ=60)
  TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCC                                                                                                                                               <  SRR19973070.1241534/150‑1 (MQ=60)
   AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGNTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                              >  SRR19973070.1691606/1‑150 (MQ=60)
   AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                              >  SRR19973070.2781021/1‑150 (MQ=60)
    AGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGC                                                                                                                                             >  SRR19973070.2599233/1‑150 (MQ=60)
       TCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGA                                                                                                                                          <  SRR19973070.932702/150‑1 (MQ=60)
       TCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGA                                                                                                                                          <  SRR19973070.932700/150‑1 (MQ=60)
       TCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGA                                                                                                                                          <  SRR19973070.932699/150‑1 (MQ=60)
        CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                         >  SRR19973070.1091610/1‑150 (MQ=60)
          AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                       <  SRR19973070.3233002/150‑1 (MQ=60)
          AGTTTTGTAGCAACAGGACAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                       >  SRR19973070.1203524/1‑150 (MQ=60)
               TGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAA                                                                                                                                  >  SRR19973070.101268/1‑150 (MQ=60)
                  AGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACC                                                                                                                               >  SRR19973070.2292719/1‑150 (MQ=60)
                       CAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCA                                                                                                                          >  SRR19973070.629050/1‑150 (MQ=60)
                             AAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCC                                                                                                                    >  SRR19973070.2653444/1‑150 (MQ=60)
                              AATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCG                                                                                                                   <  SRR19973070.2912344/150‑1 (MQ=60)
                                  CTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAA                                                                                                               >  SRR19973070.2684121/1‑150 (MQ=60)
                                   TGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAA                                                                                                              >  SRR19973070.1000571/1‑150 (MQ=60)
                                         TGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGAT                                                                                                        <  SRR19973070.3286306/150‑1 (MQ=60)
                                          GCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATG                                                                                                       <  SRR19973070.324841/150‑1 (MQ=60)
                                          GCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATG                                                                                                       <  SRR19973070.3204316/150‑1 (MQ=60)
                                              GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCACCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                                   <  SRR19973070.122604/150‑1 (MQ=60)
                                              GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                                   >  SRR19973070.499426/1‑150 (MQ=60)
                                              GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                                   >  SRR19973070.1721631/1‑150 (MQ=60)
                                                   CCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCT                                                                                              <  SRR19973070.2480544/150‑1 (MQ=60)
                                                           TGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCG                                                                                      <  SRR19973070.982118/150‑1 (MQ=60)
                                                           TGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCG                                                                                      <  SRR19973070.982463/150‑1 (MQ=60)
                                                            GAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGT                                                                                     <  SRR19973070.2599556/150‑1 (MQ=60)
                                                             AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                    >  SRR19973070.1642791/1‑150 (MQ=60)
                                                             AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                    <  SRR19973070.1186439/150‑1 (MQ=60)
                                                               CGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGC                                                                                  >  SRR19973070.1367685/1‑150 (MQ=60)
                                                               CGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGC                                                                                  >  SRR19973070.1317708/1‑150 (MQ=60)
                                                                   AAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCA                                                                              >  SRR19973070.3054036/1‑150 (MQ=60)
                                                                            TGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTA                                                                     >  SRR19973070.1699276/1‑150 (MQ=60)
                                                                                       AGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTG                                                          <  SRR19973070.2312185/150‑1 (MQ=60)
                                                                                        GCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGG                                                         <  SRR19973070.2592622/150‑1 (MQ=60)
                                                                                          ATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTT                                                       <  SRR19973070.1013872/150‑1 (MQ=60)
                                                                                          ATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTT                                                       <  SRR19973070.2955487/150‑1 (MQ=60)
                                                                                              GGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCG                                                   <  SRR19973070.2231589/150‑1 (MQ=60)
                                                                                               GAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGC                                                  >  SRR19973070.1484020/1‑150 (MQ=60)
                                                                                                AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCT                                                 >  SRR19973070.377743/1‑150 (MQ=60)
                                                                                                AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCT                                                 <  SRR19973070.3333307/150‑1 (MQ=60)
                                                                                                 ACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTC                                                >  SRR19973070.1157438/1‑150 (MQ=60)
                                                                                                 ACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTC                                                >  SRR19973070.2819427/1‑150 (MQ=60)
                                                                                                    CCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCT                                             <  SRR19973070.2833751/150‑1 (MQ=60)
                                                                                                    CCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCT                                             <  SRR19973070.3005953/150‑1 (MQ=60)
                                                                                                     CACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTC                                            <  SRR19973070.1843554/150‑1 (MQ=60)
                                                                                                      ACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGACTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTTCGCTCGCTCG                                           >  SRR19973070.2328479/1‑150 (MQ=60)
                                                                                                      ACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTGGCTCGCTCG                                           >  SRR19973070.2051316/1‑150 (MQ=60)
                                                                                                        CAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCG                                         <  SRR19973070.2797175/150‑1 (MQ=60)
                                                                                                                TCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTA                                 <  SRR19973070.2022097/150‑1 (MQ=60)
                                                                                                                 CAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAG                                <  SRR19973070.1507210/150‑1 (MQ=60)
                                                                                                                      CCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTG                           <  SRR19973070.564801/150‑1 (MQ=60)
                                                                                                                         CCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTATATTTGTCA                        >  SRR19973070.1331112/1‑150 (MQ=60)
                                                                                                                              AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTT                   <  SRR19973070.2983734/150‑1 (MQ=60)
                                                                                                                              AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTT                   >  SRR19973070.584794/1‑150 (MQ=60)
                                                                                                                              AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTT                   <  SRR19973070.2983646/150‑1 (MQ=60)
                                                                                                                               GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCAGAGAGATTAACGATGCATGGGTTTTCGCTCGCGCGCGCTGGTTTAGATTTGTGATCGGTG                  >  SRR19973070.1003067/1‑150 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG            <  SRR19973070.3139591/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG            <  SRR19973070.2694473/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG            <  SRR19973070.1032977/150‑1 (MQ=60)
                                                                                                                                     TGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAGAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTG            <  SRR19973070.1487986/150‑1 (MQ=60)
                                                                                                                                         GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT        <  SRR19973070.2638149/150‑1 (MQ=60)
                                                                                                                                            GAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAG     <  SRR19973070.1241301/150‑1 (MQ=60)
                                                                                                                                               TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC  <  SRR19973070.197233/150‑1 (MQ=60)
                                                                                                                                               TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC  <  SRR19973070.197516/150‑1 (MQ=60)
                                                                                                                                               TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC  <  SRR19973070.225685/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                 
CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC  >  CP009273/2114764‑2115056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑