Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,114,911 A→T 49.8% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,114,9110AT49.8% 17.8 / inf 333L42Q (CTG→CAG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (67/99);  new base T (69/97);  total (136/197)
Fisher's exact test for biased strand distribution p-value = 9.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCT  >  CP009273/2114762‑2115058
                                                                                                                                                     |                                                                                                                                                   
cGCTTCAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACa                                                                                                                                                     <  1:2808880/150‑1 (MQ=255)
cGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACa                                                                                                                                                     <  2:4960177/150‑1 (MQ=255)
   ttAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                                  <  1:4037663/150‑1 (MQ=255)
   ttAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                                  <  1:5005797/150‑1 (MQ=255)
    tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTcc                                                                                                                                                 >  1:693056/1‑150 (MQ=255)
     aaGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCg                                                                                                                                                >  1:3341964/1‑150 (MQ=255)
      aGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTCGAGGAATGACAGTCCGc                                                                                                                                               >  2:4448886/1‑150 (MQ=255)
       ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCt                                                                                                                                              <  1:3446158/150‑1 (MQ=255)
       ggTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCt                                                                                                                                              <  1:4960553/150‑1 (MQ=255)
        gTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCAGGTAGAGGAATGACTGTCCGCTg                                                                                                                                             >  2:5089234/1‑150 (MQ=255)
           cAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACt                                                                                                                                          <  2:4136229/150‑1 (MQ=255)
           cAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACt                                                                                                                                          >  2:2228597/1‑150 (MQ=255)
            aGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACtt                                                                                                                                         >  2:5149569/1‑150 (MQ=255)
             gTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTc                                                                                                                                        >  1:1035169/1‑150 (MQ=255)
              ttttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCg                                                                                                                                       >  2:3506572/1‑150 (MQ=255)
              ttttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCg                                                                                                                                       >  2:1629512/1‑150 (MQ=255)
                  gTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCaaa                                                                                                                                   >  1:4457527/1‑150 (MQ=255)
                    aGCAACAGGGCAAAGTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAcc                                                                                                                                 <  2:3588381/150‑1 (MQ=255)
                      cAACAGGGCAAAGTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                               <  2:4578555/150‑1 (MQ=255)
                          aGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                           >  1:2033036/1‑150 (MQ=255)
                           gggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGTTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                          >  1:1565872/1‑150 (MQ=255)
                            ggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAg                                                                                                                         <  1:983435/150‑1 (MQ=255)
                               aaaTTCTGTCGCTGCCCGCACCCCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTcc                                                                                                                      <  2:3963879/150‑1 (MQ=255)
                               aaaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTcc                                                                                                                      <  1:1141390/150‑1 (MQ=255)
                               aaaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCAGCTGACTTCGCAAACCAGCCATAGTcc                                                                                                                      >  2:2701818/1‑150 (MQ=255)
                                 aTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCgg                                                                                                                    >  2:2245547/1‑150 (MQ=255)
                                   tCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCa                                                                                                                  <  2:4372409/150‑1 (MQ=255)
                                    cTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaa                                                                                                                 <  1:1419772/150‑1 (MQ=255)
                                     tGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAAACAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCaaa                                                                                                                >  2:5181212/1‑150 (MQ=255)
                                        cGCTGCCCGAACACCGCGCCATGAGCGATACAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                             <  2:2787788/150‑1 (MQ=255)
                                        cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                             <  2:1980538/150‑1 (MQ=255)
                                        cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                             <  1:1835855/150‑1 (MQ=255)
                                        cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                             <  2:140316/150‑1 (MQ=255)
                                        cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCCTAGTCCGGCAAACat                                                                                                             >  2:2828640/1‑150 (MQ=255)
                                         gCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                            <  1:1971553/150‑1 (MQ=255)
                                         gCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                            <  2:1556317/150‑1 (MQ=255)
                                          cTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                           <  2:1442084/150‑1 (MQ=255)
                                           tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatgat                                                                                                          <  1:2636783/150‑1 (MQ=255)
                                             cccGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATgg                                                                                                        <  2:1970639/150‑1 (MQ=255)
                                               cGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTg                                                                                                      >  1:2939635/1‑150 (MQ=255)
                                                 aaCACAGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATATGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGat                                                                                                    <  2:4213679/150‑1 (MQ=255)
                                                   cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGatat                                                                                                  <  1:1799688/150‑1 (MQ=255)
                                                    acCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                                 <  2:877742/150‑1 (MQ=255)
                                                    acCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                                 <  1:3000662/150‑1 (MQ=255)
                                                    acCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                                 >  1:2979665/1‑150 (MQ=255)
                                                    acCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                                 <  1:531669/150‑1 (MQ=255)
                                                            aTGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGc                                                                                         <  1:422434/150‑1 (MQ=255)
                                                             tGAGCGAAAAAAATCGGTGATGCCTCCCAGCATCTGGAAAACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTATAGGAAGGACAGTCCGCTGACTTCGCAAACCAGCCAAACTCCGGCAAACAAGAAGGTGATATATGAAAAGCg                                                                                        >  2:3047711/1‑150 (MQ=255)
                                                                  gATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCa                                                                                   >  2:633121/1‑150 (MQ=255)
                                                                  gATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCa                                                                                   >  2:2636308/1‑150 (MQ=255)
                                                                   aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                                  <  2:323907/150‑1 (MQ=255)
                                                                   aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                                  <  2:2954279/150‑1 (MQ=255)
                                                                   aTAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAc                                                                                  <  2:160389/150‑1 (MQ=255)
                                                                      aaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCAt                                                                               >  2:1013495/1‑150 (MQ=255)
                                                                            ggTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                         >  1:4866543/1‑150 (MQ=255)
                                                                               gATGCCGCCCAGCATCTGGATCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTaa                                                                      <  2:4510222/150‑1 (MQ=255)
                                                                                aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAc                                                                     >  1:1947815/1‑150 (MQ=255)
                                                                                aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAc                                                                     <  1:544160/150‑1 (MQ=255)
                                                                                 tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATTTCTGAAAAGCGTTGCACCATAGAGTTTAACg                                                                    >  2:4424569/1‑150 (MQ=255)
                                                                                 tGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACg                                                                    <  2:170308/150‑1 (MQ=255)
                                                                                   ccgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGAt                                                                  <  1:3143368/150‑1 (MQ=255)
                                                                                   ccgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAGTAACGAt                                                                  >  1:75795/1‑150 (MQ=255)
                                                                                    cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCACCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATg                                                                 <  2:762946/150‑1 (MQ=255)
                                                                                     gccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGc                                                                >  1:2608817/1‑150 (MQ=255)
                                                                                         aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTg                                                            >  2:1487179/1‑150 (MQ=255)
                                                                                            aTCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtt                                                         <  2:1918422/150‑1 (MQ=255)
                                                                                              cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                       <  2:2528568/150‑1 (MQ=255)
                                                                                              cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                       >  1:885738/1‑150 (MQ=255)
                                                                                              cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtttt                                                       <  2:1132528/150‑1 (MQ=255)
                                                                                                ggAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                     >  1:4951559/1‑150 (MQ=255)
                                                                                                ggAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                     <  2:787090/150‑1 (MQ=255)
                                                                                                 gAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgc                                                    <  1:1693376/150‑1 (MQ=255)
                                                                                                        accaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgctcg                                             <  1:380713/150‑1 (MQ=255)
                                                                                                             cGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                        <  1:2376178/150‑1 (MQ=255)
                                                                                                             cGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                        >  1:4536954/1‑150 (MQ=255)
                                                                                                               tAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttt                                      <  2:319612/150‑1 (MQ=255)
                                                                                                                aaTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGAAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCttttt                                     <  1:824386/150‑1 (MQ=255)
                                                                                                                   cAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAg                                  >  1:2317760/1‑150 (MQ=255)
                                                                                                                      cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAtt                               >  2:477525/1‑150 (MQ=255)
                                                                                                                      cgcCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAtt                               <  2:4740699/150‑1 (MQ=255)
                                                                                                                        ccaccaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTg                             >  2:667153/1‑150 (MQ=255)
                                                                                                                         caccaACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGt                            <  1:4437961/150‑1 (MQ=255)
                                                                                                                            caACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAt                         <  1:2179630/150‑1 (MQ=255)
                                                                                                                             aaCAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATc                        >  2:4529645/1‑150 (MQ=255)
                                                                                                                               cAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgt                      >  1:2882761/1‑150 (MQ=255)
                                                                                                                                 ggTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                    <  2:4878923/150‑1 (MQ=255)
                                                                                                                                       tGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg              >  2:1803292/1‑150 (MQ=255)
                                                                                                                                       tGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTg              <  1:4809295/150‑1 (MQ=255)
                                                                                                                                          agagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtta           >  1:432407/1‑150 (MQ=255)
                                                                                                                                          agagGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtta           <  2:5104320/150‑1 (MQ=255)
                                                                                                                                           gagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttat          <  1:2471094/150‑1 (MQ=255)
                                                                                                                                            agGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatt         <  1:2183860/150‑1 (MQ=255)
                                                                                                                                            agGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatt         <  2:1329087/150‑1 (MQ=255)
                                                                                                                                             ggAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatta        <  1:1270065/150‑1 (MQ=255)
                                                                                                                                              gAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAg       <  1:3604465/150‑1 (MQ=255)
                                                                                                                                               aaTGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc      <  2:1512002/150‑1 (MQ=255)
                                                                                                                                                aTGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcc     >  1:1543337/1‑150 (MQ=255)
                                                                                                                                                  gACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcccc   >  2:2088267/1‑150 (MQ=255)
                                                                                                                                                  gACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcccc   >  1:4289761/1‑150 (MQ=255)
                                                                                                                                                   aCAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCt  <  1:3995417/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCT  >  CP009273/2114762‑2115058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A