Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,114,911 A→T 100% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,114,9110AT100.0% 702.0 / NA 212L42Q (CTG→CAG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (104/108);  total (104/108)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCC  >  CP009273/2114766‑2115055
                                                                                                                                                 |                                                                                                                                                
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCCTCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                >  2:3164304/1‑148 (MQ=255)
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                >  1:1624924/1‑148 (MQ=255)
tAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGt                                                                                                                                                >  1:1625188/1‑148 (MQ=255)
 aaGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTc                                                                                                                                               <  1:173019/148‑1 (MQ=255)
    gTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGc                                                                                                                                            <  1:1894155/148‑1 (MQ=255)
        aGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAc                                                                                                                                        >  1:868143/1‑148 (MQ=255)
        aGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAc                                                                                                                                        >  1:188178/1‑148 (MQ=255)
         gTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACt                                                                                                                                       >  2:788394/1‑148 (MQ=255)
          ttttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACtt                                                                                                                                      >  2:894215/1‑148 (MQ=255)
           tttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTc                                                                                                                                     >  1:2820123/1‑148 (MQ=255)
            ttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCg                                                                                                                                    <  2:626260/148‑1 (MQ=255)
            ttGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCg                                                                                                                                    <  1:3295759/148‑1 (MQ=255)
               tAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaa                                                                                                                                 <  1:2863143/148‑1 (MQ=255)
                aGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCaaa                                                                                                                                <  2:2047131/148‑1 (MQ=255)
                  cAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAcc                                                                                                                              <  1:1005715/148‑1 (MQ=255)
                    aCAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAg                                                                                                                            >  1:3066711/1‑148 (MQ=255)
                     cAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTTATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACAAGc                                                                                                                           >  1:1811412/1‑148 (MQ=255)
                     cAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGc                                                                                                                           >  2:3544057/1‑148 (MQ=255)
                        ggCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAt                                                                                                                        <  1:1293583/148‑1 (MQ=255)
                         gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGACTGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                       >  2:1421401/1‑148 (MQ=255)
                         gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                       <  2:2409521/148‑1 (MQ=255)
                         gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                       >  1:3030167/1‑148 (MQ=255)
                         gCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATa                                                                                                                       >  1:1232552/1‑148 (MQ=255)
                            aaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTc                                                                                                                    <  2:165163/148‑1 (MQ=255)
                            aaTTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTc                                                                                                                    <  1:2297570/148‑1 (MQ=255)
                              ttCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCg                                                                                                                  >  1:1024098/1‑148 (MQ=255)
                              ttCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCg                                                                                                                  >  2:3313115/1‑148 (MQ=255)
                               tCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCgg                                                                                                                 >  1:2247348/1‑148 (MQ=255)
                                    cGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAAc                                                                                                            >  2:1229343/1‑148 (MQ=255)
                                     gCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACa                                                                                                           <  2:2216595/148‑1 (MQ=255)
                                      cTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACat                                                                                                          >  2:2940942/1‑148 (MQ=255)
                                       tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCACCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                         <  2:2001435/148‑1 (MQ=255)
                                       tGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatg                                                                                                         <  1:570039/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                        <  1:2205870/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                        <  2:1406305/148‑1 (MQ=255)
                                        gCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACatga                                                                                                        <  2:3206975/148‑1 (MQ=255)
                                             aaCACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTg                                                                                                   >  1:2149502/1‑148 (MQ=255)
                                              acacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGa                                                                                                  >  2:2512628/1‑148 (MQ=255)
                                               cacCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGat                                                                                                 >  1:2521540/1‑148 (MQ=255)
                                                 ccGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGatat                                                                                               >  2:502488/1‑148 (MQ=255)
                                                  cgcgCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                              <  2:1897294/148‑1 (MQ=255)
                                                  cgcgCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                              >  2:2520148/1‑148 (MQ=255)
                                                  cgcgCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATc                                                                                              <  2:418972/148‑1 (MQ=255)
                                                    cgcCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTg                                                                                            <  2:550962/148‑1 (MQ=255)
                                                           aGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCg                                                                                     <  1:1702405/148‑1 (MQ=255)
                                                             cGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGtt                                                                                   >  1:1041209/1‑148 (MQ=255)
                                                             cGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGtt                                                                                   >  1:861567/1‑148 (MQ=255)
                                                                  aaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAcc                                                                              <  2:484532/148‑1 (MQ=255)
                                                                  aaaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCAcc                                                                              >  1:748906/1‑148 (MQ=255)
                                                                   aaaTCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCa                                                                             >  1:384255/1‑148 (MQ=255)
                                                                       cGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATaga                                                                         >  2:2734599/1‑148 (MQ=255)
                                                                          tGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAt                                                                      <  2:754837/148‑1 (MQ=255)
                                                                            aTGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTa                                                                    <  1:2253322/148‑1 (MQ=255)
                                                                               ccgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACg                                                                 <  2:450323/148‑1 (MQ=255)
                                                                                cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTACCGa                                                                <  2:1680192/148‑1 (MQ=255)
                                                                                cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGa                                                                <  1:145616/148‑1 (MQ=255)
                                                                                cgccCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGa                                                                <  1:3496456/148‑1 (MQ=255)
                                                                                  cccAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATCTCTGAAAAGCGTTGCACCATAGCGAGTCACGATg                                                              >  1:3342273/1‑148 (MQ=255)
                                                                                  cccAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATg                                                              >  1:3366261/1‑148 (MQ=255)
                                                                                     aGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAt                                                           >  2:3037268/1‑148 (MQ=255)
                                                                                       cATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATCGAGATTAACGATGCATTg                                                         >  2:1844070/1‑148 (MQ=255)
                                                                                        aTCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTgg                                                        <  1:2123304/148‑1 (MQ=255)
                                                                                          cTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGtt                                                      <  1:853783/148‑1 (MQ=255)
                                                                                           tGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGCTGCATTGGttt                                                     >  1:3115500/1‑148 (MQ=255)
                                                                                              aaCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                  >  1:3223206/1‑148 (MQ=255)
                                                                                              aaCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcg                                                  <  2:94568/148‑1 (MQ=255)
                                                                                              aaCACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTtcg                                                  >  2:3397673/1‑148 (MQ=255)
                                                                                               acacCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgc                                                 >  1:3077144/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGTCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                                >  1:693026/1‑148 (MQ=255)
                                                                                                caccacCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgct                                                >  1:1412152/1‑148 (MQ=255)
                                                                                                  ccaccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcg                                              >  2:1078824/1‑148 (MQ=255)
                                                                                                   caccaGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTtcgctcgc                                             <  2:1722842/148‑1 (MQ=255)
                                                                                                         cGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCgcgc                                       <  1:2448557/148‑1 (MQ=255)
                                                                                                         cGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCgcgc                                       <  1:3314170/148‑1 (MQ=255)
                                                                                                           tAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtt                                     <  2:1774228/148‑1 (MQ=255)
                                                                                                             aTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCtttt                                   >  2:1734305/1‑148 (MQ=255)
                                                                                                             aTCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCGCGCGCtttt                                   >  2:3493464/1‑148 (MQ=255)
                                                                                                                  cgcCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTTCGCTCGCTGGCGCTTTTTTAGa                              >  1:1206229/1‑148 (MQ=255)
                                                                                                                       ccaACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGt                         <  1:1832438/148‑1 (MQ=255)
                                                                                                                          aCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAt                      >  2:406730/1‑148 (MQ=255)
                                                                                                                          aCAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCAt                      <  2:3430869/148‑1 (MQ=255)
                                                                                                                              gTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgtt                  <  1:2734599/148‑1 (MQ=255)
                                                                                                                              gTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgtt                  >  1:417403/1‑148 (MQ=255)
                                                                                                                               tGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCgttg                 >  1:531982/1‑148 (MQ=255)
                                                                                                                               tGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAGTTTTCATCgttg                 >  1:625790/1‑148 (MQ=255)
                                                                                                                                  aTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTc              >  1:2907989/1‑148 (MQ=255)
                                                                                                                                  aTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTc              <  2:3082324/148‑1 (MQ=255)
                                                                                                                                  aTGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTc              <  2:1172081/148‑1 (MQ=255)
                                                                                                                                      agagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTGCCTGt          >  1:912800/1‑148 (MQ=255)
                                                                                                                                       gagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtt         <  1:2648759/148‑1 (MQ=255)
                                                                                                                                       gagGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtt         <  1:535022/148‑1 (MQ=255)
                                                                                                                                        agGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtta        <  2:3246233/148‑1 (MQ=255)
                                                                                                                                        agGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGtta        >  1:379556/1‑148 (MQ=255)
                                                                                                                                         ggAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttat       >  1:530287/1‑148 (MQ=255)
                                                                                                                                           aaTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGttatta     <  2:1950063/148‑1 (MQ=255)
                                                                                                                                            aTGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAg    <  2:2134063/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc   <  1:1536256/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc   <  1:602155/148‑1 (MQ=255)
                                                                                                                                             tGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGc   <  2:2344875/148‑1 (MQ=255)
                                                                                                                                              gACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGcc  <  2:1391246/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCC  >  CP009273/2114766‑2115055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  CP009273/2114763‑2115060
                                                                                                                                                    |                                                                                                                                                     
GCTTAAGGTCCAGTTTTGTAGCAACAGTGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCTTGTAGAGGAATGACTG                                                                                                                                                      <  SRR19973067.1576002/150‑1 (MQ=60)
 CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     >  SRR19973067.1636331/1‑150 (MQ=60)
 CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     >  SRR19973067.1636072/1‑150 (MQ=60)
 CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     >  SRR19973067.1636337/1‑150 (MQ=60)
 CTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGT                                                                                                                                                     >  SRR19973067.1683565/1‑150 (MQ=60)
    AAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCG                                                                                                                                                  <  SRR19973067.174416/150‑1 (MQ=60)
       GTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTG                                                                                                                                               <  SRR19973067.1907080/150‑1 (MQ=60)
       GTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTG                                                                                                                                               <  SRR19973067.1907072/150‑1 (MQ=60)
       GTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTG                                                                                                                                               <  SRR19973067.2042923/150‑1 (MQ=60)
         CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                             >  SRR19973067.873948/1‑150 (MQ=60)
         CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                             >  SRR19973067.189648/1‑150 (MQ=60)
         CCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGAC                                                                                                                                             >  SRR19973067.873951/1‑150 (MQ=60)
           AGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTT                                                                                                                                           >  SRR19973067.3509096/1‑150 (MQ=60)
            GTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTC                                                                                                                                          >  SRR19973067.2148315/1‑150 (MQ=60)
            GTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTC                                                                                                                                          >  SRR19973067.2838275/1‑150 (MQ=60)
               TTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCA                                                                                                                                       <  SRR19973067.3316502/150‑1 (MQ=60)
                TGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAA                                                                                                                                      >  SRR19973067.3327859/1‑150 (MQ=60)
                TGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAA                                                                                                                                      >  SRR19973067.1040028/1‑150 (MQ=60)
                  TAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAAC                                                                                                                                    <  SRR19973067.2881490/150‑1 (MQ=60)
                     CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                                 >  SRR19973067.3086229/1‑150 (MQ=60)
                     CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                                 >  SRR19973067.3086492/1‑150 (MQ=60)
                     CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                                 <  SRR19973067.1012640/150‑1 (MQ=60)
                     CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                                 <  SRR19973067.221774/150‑1 (MQ=60)
                     CAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAG                                                                                                                                 <  SRR19973067.1012625/150‑1 (MQ=60)
                      AACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTTATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACAAGC                                                                                                                                >  SRR19973067.1823664/1‑150 (MQ=60)
                      AACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGC                                                                                                                                >  SRR19973067.1823796/1‑150 (MQ=60)
                         AGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCAT                                                                                                                             >  SRR19973067.1963342/1‑150 (MQ=60)
                          GGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATA                                                                                                                            >  SRR19973067.3049461/1‑150 (MQ=60)
                          GGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATA                                                                                                                            >  SRR19973067.1240805/1‑150 (MQ=60)
                          GGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCAACAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATA                                                                                                                            <  SRR19973067.662061/150‑1 (MQ=60)
                           GGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAG                                                                                                                           <  SRR19973067.1302412/150‑1 (MQ=60)
                               AATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAGATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCG                                                                                                                       <  SRR19973067.2312737/150‑1 (MQ=60)
                               AATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCG                                                                                                                       <  SRR19973067.2312726/150‑1 (MQ=60)
                               AATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCG                                                                                                                       >  SRR19973067.1031139/1‑150 (MQ=60)
                                ATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGG                                                                                                                      >  SRR19973067.2262274/1‑150 (MQ=60)
                                          TGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGAT                                                                                                            <  SRR19973067.573736/150‑1 (MQ=60)
                                           GCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATG                                                                                                           <  SRR19973067.420637/150‑1 (MQ=60)
                                           GCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATG                                                                                                           <  SRR19973067.2220581/150‑1 (MQ=60)
                                              CGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTG                                                                                                        >  SRR19973067.2163871/1‑150 (MQ=60)
                                               GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                                       >  SRR19973067.400764/1‑150 (MQ=60)
                                               GAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGA                                                                                                       >  SRR19973067.321102/1‑150 (MQ=60)
                                                AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      >  SRR19973067.2538108/1‑150 (MQ=60)
                                                AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      >  SRR19973067.2538091/1‑150 (MQ=60)
                                                AACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGAT                                                                                                      >  SRR19973067.2538070/1‑150 (MQ=60)
                                                       CGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAA                                                                                               >  SRR19973067.1179851/1‑150 (MQ=60)
                                                           ATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGC                                                                                           >  SRR19973067.3385839/1‑150 (MQ=60)
                                                              AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                        >  SRR19973067.867214/1‑150 (MQ=60)
                                                              AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                        >  SRR19973067.867348/1‑150 (MQ=60)
                                                              AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                        >  SRR19973067.1048369/1‑150 (MQ=60)
                                                              AGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTT                                                                                        <  SRR19973067.1714096/150‑1 (MQ=60)
                                                                   TAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACC                                                                                   >  SRR19973067.754100/1‑150 (MQ=60)
                                                                    AAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCA                                                                                  >  SRR19973067.3059266/1‑150 (MQ=60)
                                                                    AAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCA                                                                                  >  SRR19973067.386935/1‑150 (MQ=60)
                                                                           GGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGAT                                                                           <  SRR19973067.1498227/150‑1 (MQ=60)
                                                                            GTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATT                                                                          <  SRR19973067.714413/150‑1 (MQ=60)
                                                                            GTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATT                                                                          <  SRR19973067.2855849/150‑1 (MQ=60)
                                                                               ATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAC                                                                       <  SRR19973067.2268281/150‑1 (MQ=60)
                                                                               ATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAAC                                                                       <  SRR19973067.2268283/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGNTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGNTG                                                                   <  SRR19973067.2247984/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATCTCTGAAAAGCGTTGCACCATAGCGAGTCACGATG                                                                   >  SRR19973067.3363446/1‑150 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973067.1820381/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   >  SRR19973067.3363436/1‑150 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   >  SRR19973067.3387632/1‑150 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   >  SRR19973067.1616279/1‑150 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973067.2923373/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973067.3518636/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973067.144028/150‑1 (MQ=60)
                                                                                   CGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATG                                                                   <  SRR19973067.146882/150‑1 (MQ=60)
                                                                                        AGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTG                                                              >  SRR19973067.698113/1‑150 (MQ=60)
                                                                                           ATCTGTAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTT                                                           <  SRR19973067.2700520/150‑1 (MQ=60)
                                                                                           ATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTT                                                           <  SRR19973067.2137470/150‑1 (MQ=60)
                                                                                            TCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGCTGCATTGGTTT                                                          >  SRR19973067.3135306/1‑150 (MQ=60)
                                                                                            TCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTT                                                          >  SRR19973067.3134977/1‑150 (MQ=60)
                                                                                             CTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTT                                                         <  SRR19973067.859544/150‑1 (MQ=60)
                                                                                              TGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTC                                                        <  SRR19973067.616350/150‑1 (MQ=60)
                                                                                               GGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCG                                                       <  SRR19973067.386431/150‑1 (MQ=60)
                                                                                               GGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCG                                                       >  SRR19973067.3243461/1‑150 (MQ=60)
                                                                                                GAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATGGGTTTTCGC                                                      >  SRR19973067.3096728/1‑150 (MQ=60)
                                                                                                 AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGTCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCT                                                     >  SRR19973067.697804/1‑150 (MQ=60)
                                                                                                 AACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCT                                                     >  SRR19973067.1421713/1‑150 (MQ=60)
                                                                                                          AGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGC                                            >  SRR19973067.1561958/1‑150 (MQ=60)
                                                                                                            CGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTT                                          <  SRR19973067.3335121/150‑1 (MQ=60)
                                                                                                            CGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTT                                          <  SRR19973067.2464644/150‑1 (MQ=60)
                                                                                                             GTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTT                                         <  SRR19973067.2763882/150‑1 (MQ=60)
                                                                                                                   AGCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTTCGCTCGCTGGCGCTTTTTTAGA                                   >  SRR19973067.1214132/1‑150 (MQ=60)
                                                                                                                    GCGCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAT                                  <  SRR19973067.2290778/150‑1 (MQ=60)
                                                                                                                      GCCACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTT                                <  SRR19973067.1729934/150‑1 (MQ=60)
                                                                                                                        CACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTATATTTTT                              >  SRR19973067.2467562/1‑150 (MQ=60)
                                                                                                                        CACCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGT                              >  SRR19973067.903728/1‑150 (MQ=60)
                                                                                                                          CCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCA                            <  SRR19973067.1846189/150‑1 (MQ=60)
                                                                                                                          CCAACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCA                            <  SRR19973067.1844909/150‑1 (MQ=60)
                                                                                                                            AACAGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGAAAACATGATGGTGATATCTGAAAAGCGTTGCACCATTGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATC                          <  SRR19973067.3138181/150‑1 (MQ=60)
                                                                                                                               AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTT                       >  SRR19973067.420279/1‑150 (MQ=60)
                                                                                                                               AGGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCGCGCTCGCGCTTTTTTAGATTTGTCATCGTT                       >  SRR19973067.420301/1‑150 (MQ=60)
                                                                                                                                GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTG                      >  SRR19973067.534950/1‑150 (MQ=60)
                                                                                                                                GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTG                      >  SRR19973067.535547/1‑150 (MQ=60)
                                                                                                                                GGTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAGTTTTCATCGTTG                      >  SRR19973067.629936/1‑150 (MQ=60)
                                                                                                                                 GTGCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGT                     <  SRR19973067.2752250/150‑1 (MQ=60)
                                                                                                                                   GCATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTC                   >  SRR19973067.2926519/1‑150 (MQ=60)
                                                                                                                                    CATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCC                  <  SRR19973067.1237567/150‑1 (MQ=60)
                                                                                                                                     ATGTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCT                 >  SRR19973067.1299046/1‑150 (MQ=60)
                                                                                                                                       GTAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTGCCTGT               >  SRR19973067.918874/1‑150 (MQ=60)
                                                                                                                                        TAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTT              <  SRR19973067.3029256/150‑1 (MQ=60)
                                                                                                                                        TAGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTT              <  SRR19973067.813187/150‑1 (MQ=60)
                                                                                                                                         AGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTA             >  SRR19973067.382192/1‑150 (MQ=60)
                                                                                                                                         AGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTA             >  SRR19973067.382199/1‑150 (MQ=60)
                                                                                                                                         AGAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCGTTTTTAGATTTGTCATCGTTGTTCCTGTTA             >  SRR19973067.3247700/1‑150 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            <  SRR19973067.538602/150‑1 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            <  SRR19973067.2665848/150‑1 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            <  SRR19973067.1323881/150‑1 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            >  SRR19973067.533846/1‑150 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            <  SRR19973067.540166/150‑1 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAT            <  SRR19973067.538505/150‑1 (MQ=60)
                                                                                                                                          GAGGAATGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCAGTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTGATCGGTGTTGCTGTTAT            >  SRR19973067.2096231/1‑150 (MQ=60)
                                                                                                                                                TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC      <  SRR19973067.1546660/150‑1 (MQ=60)
                                                                                                                                                TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC      <  SRR19973067.605963/150‑1 (MQ=60)
                                                                                                                                                TGACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCC      <  SRR19973067.605768/150‑1 (MQ=60)
                                                                                                                                                 GACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCGTGTTATTAGCCCC     >  SRR19973067.789332/1‑150 (MQ=60)
                                                                                                                                                 GACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCGGTTATTAGCCCC     >  SRR19973067.789416/1‑150 (MQ=60)
                                                                                                                                                  ACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCT    <  SRR19973067.3334054/150‑1 (MQ=60)
                                                                                                                                                  ACTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCT    <  SRR19973067.1105974/150‑1 (MQ=60)
                                                                                                                                                   CTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTT   <  SRR19973067.636677/150‑1 (MQ=60)
                                                                                                                                                   CTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTT   <  SRR19973067.3310162/150‑1 (MQ=60)
                                                                                                                                                   CTGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTT   <  SRR19973067.2433113/150‑1 (MQ=60)
                                                                                                                                                    TGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  SRR19973067.1534070/1‑150 (MQ=60)
                                                                                                                                                    TGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  SRR19973067.2902620/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAATCGGTGATGCCGCCCAGCATCTGGAACACCACCAGCGTAATCAGCGCCACCAACAGGTGCATGTAGAGGAATGACAGTCCGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTA  >  CP009273/2114763‑2115060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑