Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,062,424 G→T 100% I71I (ATC→ATA)  yihQ ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,062,4240GT97.9% 964.9 / 9.7 288I71I (ATC→ATA) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (131/151);  total (134/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 288 total aligned reads displayed.

GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATG  >  CP009273/4062277‑4062570
                                                                                                                                                   |                                                                                                                                                  
  gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATgg                                                                                                                                                >  1:1020354/1‑150 (MQ=255)
  gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATgg                                                                                                                                                <  1:1507229/150‑1 (MQ=255)
    aGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCg                                                                                                                                              >  2:594660/1‑150 (MQ=255)
     gCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGt                                                                                                                                             <  2:1728614/150‑1 (MQ=255)
       cAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCg                                                                                                                                           >  1:4622480/1‑150 (MQ=255)
         gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                         <  2:187753/150‑1 (MQ=255)
          aTACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTa                                                                                                                                        <  2:2965948/150‑1 (MQ=255)
          aTACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTa                                                                                                                                        <  2:4139392/150‑1 (MQ=255)
            aCGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAg                                                                                                                                      >  2:2234679/1‑150 (MQ=255)
              ggTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcg                                                                                                                                    <  2:4618522/150‑1 (MQ=255)
              ggTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcg                                                                                                                                    >  1:288379/1‑150 (MQ=255)
              ggTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcg                                                                                                                                    <  2:3071588/150‑1 (MQ=255)
                  gtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAt                                                                                                                                >  1:2589949/1‑150 (MQ=255)
                        aaGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtctc                                                                                                                          >  1:3630124/1‑150 (MQ=255)
                        aaGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtctc                                                                                                                          >  1:2079677/1‑150 (MQ=255)
                           gTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTg                                                                                                                       <  2:287710/150‑1 (MQ=255)
                               tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGt                                                                                                                   <  1:1436098/150‑1 (MQ=255)
                                 gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                 <  1:3717102/150‑1 (MQ=255)
                                 gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                 >  1:2513218/1‑150 (MQ=255)
                                 gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                 >  2:865845/1‑150 (MQ=255)
                                 gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                 <  1:1990567/150‑1 (MQ=255)
                                   tttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTAt                                                                                                               >  2:2922483/1‑150 (MQ=255)
                                   tttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTAt                                                                                                               >  2:2408428/1‑150 (MQ=255)
                                        aGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTCAGCGCAATTTTCTCCTGTAGTTTATCTTTa                                                                                                          <  1:2379403/150‑1 (MQ=255)
                                        aGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTa                                                                                                          <  1:2383575/150‑1 (MQ=255)
                                          ttCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAt                                                                                                        <  2:2354560/150‑1 (MQ=255)
                                           tCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                                                       <  2:1408269/150‑1 (MQ=255)
                                               gCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGa                                                                                                   <  2:3201228/150‑1 (MQ=255)
                                                cAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                                  <  1:4452265/150‑1 (MQ=255)
                                                cAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                                  <  2:4033542/150‑1 (MQ=255)
                                                   tAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAtt                                                                                               <  1:3254466/150‑1 (MQ=255)
                                                    aaaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTa                                                                                              <  2:2492807/150‑1 (MQ=255)
                                                     aaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAc                                                                                             <  2:4411909/150‑1 (MQ=255)
                                                      aCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAcc                                                                                            <  1:2185295/150‑1 (MQ=255)
                                                      aCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAcc                                                                                            <  1:3509407/150‑1 (MQ=255)
                                                           ccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGa                                                                                       >  1:3712851/1‑150 (MQ=255)
                                                            cTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                      <  2:3636616/150‑1 (MQ=255)
                                                            cTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                      >  2:494826/1‑150 (MQ=255)
                                                                tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACata                                                                                  >  1:4070186/1‑150 (MQ=255)
                                                                tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACata                                                                                  >  2:4550267/1‑150 (MQ=255)
                                                                 cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                                 <  1:3166617/150‑1 (MQ=255)
                                                                 cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                                 >  2:1277288/1‑150 (MQ=255)
                                                                   tcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCg                                                                               >  1:4596800/1‑150 (MQ=255)
                                                                   tcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCg                                                                               <  2:2637865/150‑1 (MQ=255)
                                                                    cgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGa                                                                              >  2:2250032/1‑150 (MQ=255)
                                                                     ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                             <  2:4183356/150‑1 (MQ=255)
                                                                     ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                             >  1:797493/1‑150 (MQ=255)
                                                                        agagATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                          >  2:1268575/1‑150 (MQ=255)
                                                                         gagaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                                                         >  1:2734978/1‑150 (MQ=255)
                                                                         gagaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                                                         <  2:663448/150‑1 (MQ=255)
                                                                            atatTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCt                                                                      <  2:2558547/150‑1 (MQ=255)
                                                                                tCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAc                                                                  <  2:2144319/150‑1 (MQ=255)
                                                                                 cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAcc                                                                 >  1:2504675/1‑150 (MQ=255)
                                                                                    cGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGa                                                              >  2:132308/1‑150 (MQ=255)
                                                                                      tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAAGCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGc                                                            >  2:2618551/1‑150 (MQ=255)
                                                                                          gcTAATGTCAGAACCACGGCTGATATGAATTAACCAACCATACGGTGACTGGCTGACTATGGCGTCTGTACTCGCTATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTTCCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                        <  1:2446979/150‑1 (MQ=255)
                                                                                          gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                        >  1:616341/1‑150 (MQ=255)
                                                                                          gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                        >  1:1326553/1‑150 (MQ=255)
                                                                                          gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                        >  1:2685893/1‑150 (MQ=255)
                                                                                             aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                     <  1:2014314/150‑1 (MQ=255)
                                                                                              aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAt                                                    <  2:2584452/150‑1 (MQ=255)
                                                                                                 tCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa                                                 >  1:231935/1‑150 (MQ=255)
                                                                                                  cAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAAc                                                <  2:619211/150‑1 (MQ=255)
                                                                                                    gAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACaa                                              >  2:2696212/1‑150 (MQ=255)
                                                                                                     aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                             <  1:1045667/150‑1 (MQ=255)
                                                                                                     aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                             <  2:1564321/150‑1 (MQ=255)
                                                                                                        cACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAt                                          >  1:3882497/1‑150 (MQ=255)
                                                                                                          cGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                        <  2:1908877/150‑1 (MQ=255)
                                                                                                           ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAt                                       <  2:3000676/150‑1 (MQ=255)
                                                                                                           ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAt                                       >  1:600887/1‑150 (MQ=255)
                                                                                                           ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGATTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAt                                       <  2:1092775/150‑1 (MQ=255)
                                                                                                              tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                    <  2:2513218/150‑1 (MQ=255)
                                                                                                              tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                    >  2:16514/1‑150 (MQ=255)
                                                                                                               gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCttt                                   >  2:2056225/1‑150 (MQ=255)
                                                                                                                aaaTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                                  >  1:72392/1‑150 (MQ=255)
                                                                                                                  aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCt                                <  1:1585150/150‑1 (MQ=255)
                                                                                                                      aTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                            <  2:575823/150‑1 (MQ=255)
                                                                                                                      aTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                            >  1:3864072/1‑150 (MQ=255)
                                                                                                                       ttAACCTACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTTTCTTTGCTATggg                           <  1:1395958/150‑1 (MQ=255)
                                                                                                                        tAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGt                          <  2:3499077/150‑1 (MQ=255)
                                                                                                                         aaccaaccATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGtt                         >  2:4586757/1‑150 (MQ=255)
                                                                                                                          accaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTa                        <  1:1388479/150‑1 (MQ=255)
                                                                                                                          accaaccaTCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTa                        >  1:2476800/1‑150 (MQ=255)
                                                                                                                             aaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaaa                     >  1:2144024/1‑150 (MQ=255)
                                                                                                                              accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAt                    >  2:3564889/1‑150 (MQ=255)
                                                                                                                              accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAt                    >  1:4460081/1‑150 (MQ=255)
                                                                                                                               ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATa                   >  1:1189820/1‑150 (MQ=255)
                                                                                                                                caTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                  >  1:167919/1‑150 (MQ=255)
                                                                                                                                caTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                  <  2:2469186/150‑1 (MQ=255)
                                                                                                                                caTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                  <  2:4487778/150‑1 (MQ=255)
                                                                                                                                     ggTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgt             >  2:4462649/1‑150 (MQ=255)
                                                                                                                                      gTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgtt            <  1:4298931/150‑1 (MQ=255)
                                                                                                                                       tGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttg           <  2:2707318/150‑1 (MQ=255)
                                                                                                                                        gACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt          >  2:3370630/1‑150 (MQ=255)
                                                                                                                                        gACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt          <  2:1179353/150‑1 (MQ=255)
                                                                                                                                         aCTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgtt         >  1:2698791/1‑150 (MQ=255)
                                                                                                                                         aCTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgctgtt         <  1:1071583/150‑1 (MQ=255)
                                                                                                                                          cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGttgttg        <  1:2234679/150‑1 (MQ=255)
                                                                                                                                          cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATACGCTATACCTGAGCCAATCCATAAAAAAGGATTATCTTTGCTATGGGTTAAAATAATAATttgttg        >  1:519639/1‑150 (MQ=255)
                                                                                                                                            ggCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGaa      >  2:599043/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATG  >  CP009273/4062277‑4062570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGT  >  CP009273/4062275‑4062571
                                                                                                                                                     |                                                                                                                                                   
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                     <  SRR19973072.949533/150‑1 (MQ=60)
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                     >  SRR19973072.3200017/1‑150 (MQ=60)
  GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTAT                                                                                                                                                   <  SRR19973072.1023109/150‑1 (MQ=60)
  GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTAT                                                                                                                                                   <  SRR19973072.2730511/150‑1 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                 >  SRR19973072.4421193/1‑150 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                 <  SRR19973072.1510434/150‑1 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                 <  SRR19973072.1510437/150‑1 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                 >  SRR19973072.1022569/1‑150 (MQ=60)
        CCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTC                                                                                                                                             <  SRR19973072.1145099/150‑1 (MQ=60)
        CCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTC                                                                                                                                             <  SRR19973072.3833357/150‑1 (MQ=60)
         CAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCG                                                                                                                                            >  SRR19973072.4632163/1‑150 (MQ=60)
         CAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCG                                                                                                                                            >  SRR19973072.4632224/1‑150 (MQ=60)
               CGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGC                                                                                                                                      >  SRR19973072.2474550/1‑150 (MQ=60)
               CGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGC                                                                                                                                      >  SRR19973072.556003/1‑150 (MQ=60)
                GGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCG                                                                                                                                     >  SRR19973072.289059/1‑150 (MQ=60)
                    GTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAT                                                                                                                                 >  SRR19973072.2595504/1‑150 (MQ=60)
                    GTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAT                                                                                                                                 >  SRR19973072.2595539/1‑150 (MQ=60)
                         TAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCT                                                                                                                            <  SRR19973072.2455966/150‑1 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.3637755/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.2084190/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.3637775/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.2084212/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.453570/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAAAGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                           >  SRR19973072.4218744/1‑150 (MQ=60)
                              TTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGT                                                                                                                       <  SRR19973072.2450868/150‑1 (MQ=60)
                                 TCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGT                                                                                                                    >  SRR19973072.2267797/1‑150 (MQ=60)
                                 TCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGT                                                                                                                    <  SRR19973072.1439199/150‑1 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  >  SRR19973072.3559122/1‑150 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  <  SRR19973072.2120489/150‑1 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  >  SRR19973072.2518680/1‑150 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  <  SRR19973072.1994849/150‑1 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  >  SRR19973072.2518648/1‑150 (MQ=60)
                                   GTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTT                                                                                                                  <  SRR19973072.3724925/150‑1 (MQ=60)
                                    TTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTA                                                                                                                 >  SRR19973072.1473612/1‑150 (MQ=60)
                                       TGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCT                                                                                                              >  SRR19973072.4350992/1‑150 (MQ=60)
                                        GTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTT                                                                                                             <  SRR19973072.1383957/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTCAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                           <  SRR19973072.2384533/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                           <  SRR19973072.2388723/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                           <  SRR19973072.2388548/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                           <  SRR19973072.2384556/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                           <  SRR19973072.676746/150‑1 (MQ=60)
                                              CCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGC                                                                                                       >  SRR19973072.2214969/1‑150 (MQ=60)
                                                 GCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGA                                                                                                    <  SRR19973072.3881985/150‑1 (MQ=60)
                                                  CAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAA                                                                                                   <  SRR19973072.4461653/150‑1 (MQ=60)
                                                   AATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAA                                                                                                  >  SRR19973072.1758371/1‑150 (MQ=60)
                                                    ATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAT                                                                                                 <  SRR19973072.2001673/150‑1 (MQ=60)
                                                    ATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAT                                                                                                 <  SRR19973072.3679347/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                <  SRR19973072.3261392/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                <  SRR19973072.3394146/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                >  SRR19973072.155738/1‑150 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                <  SRR19973072.3261374/150‑1 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                             <  SRR19973072.2190048/150‑1 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                             <  SRR19973072.3516768/150‑1 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                          <  SRR19973072.1164446/150‑1 (MQ=60)
                                                            CCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGG                                                                                         >  SRR19973072.2009011/1‑150 (MQ=60)
                                                             CCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGA                                                                                        >  SRR19973072.3720669/1‑150 (MQ=60)
                                                                GATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACA                                                                                     >  SRR19973072.3719606/1‑150 (MQ=60)
                                                                GATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACA                                                                                     <  SRR19973072.2082617/150‑1 (MQ=60)
                                                                 ATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACAT                                                                                    <  SRR19973072.3174599/150‑1 (MQ=60)
                                                                  TCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATA                                                                                   >  SRR19973072.4078726/1‑150 (MQ=60)
                                                                  TCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATA                                                                                   <  SRR19973072.762130/150‑1 (MQ=60)
                                                                   CGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATAT                                                                                  <  SRR19973072.3173300/150‑1 (MQ=60)
                                                                   CGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATAT                                                                                  <  SRR19973072.1218311/150‑1 (MQ=60)
                                                                   CGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATAT                                                                                  <  SRR19973072.3256448/150‑1 (MQ=60)
                                                                    GTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATCTC                                                                                 >  SRR19973072.458464/1‑150 (MQ=60)
                                                                    GTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATC                                                                                 >  SRR19973072.2849649/1‑150 (MQ=60)
                                                                     TCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCG                                                                                >  SRR19973072.4606448/1‑150 (MQ=60)
                                                                     TCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCG                                                                                >  SRR19973072.4606475/1‑150 (MQ=60)
                                                                     TCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCG                                                                                >  SRR19973072.29470/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                              >  SRR19973072.799194/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                              <  SRR19973072.2752016/150‑1 (MQ=60)
                                                                        GCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATA                                                                             >  SRR19973072.2930939/1‑150 (MQ=60)
                                                                         CAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTACCCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATAT                                                                            <  SRR19973072.1394841/150‑1 (MQ=60)
                                                                         CAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATAT                                                                            <  SRR19973072.1394906/150‑1 (MQ=60)
                                                                         CAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATAT                                                                            <  SRR19973072.2418254/150‑1 (MQ=60)
                                                                          AGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATC                                                                           >  SRR19973072.2347365/1‑150 (MQ=60)
                                                                           GAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCC                                                                          >  SRR19973072.2740768/1‑150 (MQ=60)
                                                                           GAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCC                                                                          >  SRR19973072.2740814/1‑150 (MQ=60)
                                                                            AGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCG                                                                         <  SRR19973072.2512100/150‑1 (MQ=60)
                                                                                 TTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATA                                                                    <  SRR19973072.509001/150‑1 (MQ=60)
                                                                                   CAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACC                                                                  >  SRR19973072.2510120/1‑150 (MQ=60)
                                                                                   CAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACC                                                                  >  SRR19973072.2510081/1‑150 (MQ=60)
                                                                                   CAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACC                                                                  >  SRR19973072.2510102/1‑150 (MQ=60)
                                                                                    AGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCT                                                                 <  SRR19973072.1239072/150‑1 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGATATGAATTAACCAACCATACGGTGACTGGCTGACTATGGCGTCTGTACTCGCTATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTTCCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         <  SRR19973072.2452301/150‑1 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.1329454/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.2136451/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.2691616/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.617657/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.617708/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                         >  SRR19973072.617759/1‑150 (MQ=60)
                                                                                               AATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCA                                                      >  SRR19973072.389411/1‑150 (MQ=60)
                                                                                               AATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCA                                                      <  SRR19973072.2018661/150‑1 (MQ=60)
                                                                                                  GTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAA                                                   >  SRR19973072.3809395/1‑150 (MQ=60)
                                                                                                   TCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAA                                                  >  SRR19973072.232509/1‑150 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                              <  SRR19973072.1047919/150‑1 (MQ=60)
                                                                                                          CACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAT                                           >  SRR19973072.3890571/1‑150 (MQ=60)
                                                                                                           ACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATT                                          <  SRR19973072.3962729/150‑1 (MQ=60)
                                                                                                             GGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAT                                        >  SRR19973072.602273/1‑150 (MQ=60)
                                                                                                                  AAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTG                                   >  SRR19973072.72581/1‑150 (MQ=60)
                                                                                                                  AAATGAATTAACCAACCATCCGGAGACTGGCTGACTAATGCGTCGGTAAGCGCAATTTTCACCTGTAGTTTATCTTTAATGCTGAAATAACCGAGGAACATATAGATATCCGATATAACTGAGCCAATCCATAAACAAGGAAAATCTTTG                                   >  SRR19973072.72580/1‑150 (MQ=60)
                                                                                                                    ATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCT                                 <  SRR19973072.1588552/150‑1 (MQ=60)
                                                                                                                     TGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTA                                >  SRR19973072.2566684/1‑150 (MQ=60)
                                                                                                                     TGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTA                                >  SRR19973072.1349882/1‑150 (MQ=60)
                                                                                                                     TGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGACTATCTTTGCTA                                >  SRR19973072.2172393/1‑150 (MQ=60)
                                                                                                                        ATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGG                             <  SRR19973072.2378873/150‑1 (MQ=60)
                                                                                                                        ATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGG                             >  SRR19973072.3872110/1‑150 (MQ=60)
                                                                                                                         TTAACCTACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTTTCTTTGCTATGGG                            <  SRR19973072.1398999/150‑1 (MQ=60)
                                                                                                                         TTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGG                            >  SRR19973072.1430688/1‑150 (MQ=60)
                                                                                                                         TTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGG                            >  SRR19973072.1430794/1‑150 (MQ=60)
                                                                                                                          TAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGT                           >  SRR19973072.4239448/1‑150 (MQ=60)
                                                                                                                           AACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTT                          >  SRR19973072.218725/1‑150 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGTCTTTGGCGTCGGTTAGCGAAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCTCTATACCTGAGCCACTCCATAAACAATGATTATCTTTGCTATGGGTTA                         <  SRR19973072.1650023/150‑1 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         >  SRR19973072.2200539/1‑150 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         <  SRR19973072.1391502/150‑1 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         <  SRR19973072.3720756/150‑1 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         <  SRR19973072.1650362/150‑1 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         <  SRR19973072.1982241/150‑1 (MQ=60)
                                                                                                                            ACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTA                         >  SRR19973072.2482173/1‑150 (MQ=60)
                                                                                                                               AACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAA                      >  SRR19973072.2659811/1‑150 (MQ=60)
                                                                                                                               AACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAA                      >  SRR19973072.2148690/1‑150 (MQ=60)
                                                                                                                               AACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAA                      >  SRR19973072.2659826/1‑150 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                     >  SRR19973072.4469487/1‑150 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                     >  SRR19973072.4469029/1‑150 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                     >  SRR19973072.4469492/1‑150 (MQ=60)
                                                                                                                                 CCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATA                    >  SRR19973072.1192339/1‑150 (MQ=60)
                                                                                                                                  CATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAA                   >  SRR19973072.168348/1‑150 (MQ=60)
                                                                                                                                    TCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGA                 <  SRR19973072.58468/150‑1 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT             <  SRR19973072.4307901/150‑1 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT             >  SRR19973072.745563/1‑150 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGTCGTTGTT          >  SRR19973072.2704521/1‑150 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT          <  SRR19973072.1073866/150‑1 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT          >  SRR19973072.2704533/1‑150 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT          <  SRR19973072.1073865/150‑1 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGCTGTT          <  SRR19973072.1073869/150‑1 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTG         <  SRR19973072.2239512/150‑1 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATACGCTATACCTGAGCCAATCCATAAAAAAGGATTATCTTTGCTATGGGTTAAAATAAtaatttgttg         >  SRR19973072.520879/1‑140 (MQ=60)
                                                                                                                                                 TGACTATGGCGTCGGTTAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAAT    <  SRR19973072.3885709/150‑1 (MQ=60)
                                                                                                                                                   ACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGT  <  SRR19973072.1306138/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGT  >  CP009273/4062275‑4062571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑