Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,062,424 G→T 100% I71I (ATC→ATA)  yihQ ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,062,4240GT100.0% 770.8 / NA 230I71I (ATC→ATA) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (112/118);  total (112/118)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

CAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAA  >  CP009273/4062280‑4062566
                                                                                                                                                |                                                                                                                                              
      gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCg                                                                                                                                       >  1:360035/1‑148 (MQ=255)
        tACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                     <  2:3167913/148‑1 (MQ=255)
        tACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                     <  1:1989118/148‑1 (MQ=255)
         aCGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTa                                                                                                                                    >  2:2374815/1‑148 (MQ=255)
             ttGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcg                                                                                                                                >  2:2180032/1‑148 (MQ=255)
              tgtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGAGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcgc                                                                                                                               >  2:1389220/1‑148 (MQ=255)
              tgtgGTTAAGGTTGTCGTTTTGTAGTTCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcgc                                                                                                                               <  1:2664798/148‑1 (MQ=255)
                    tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                         >  1:1015691/1‑148 (MQ=255)
                    tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                         >  1:1182537/1‑148 (MQ=255)
                        gTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTcc                                                                                                                     >  2:65422/1‑148 (MQ=255)
                        gTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTcc                                                                                                                     <  2:2866252/148‑1 (MQ=255)
                         ttGTCGTTTTGTATTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCt                                                                                                                    >  1:783462/1‑148 (MQ=255)
                         ttGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTTACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCt                                                                                                                    >  1:407067/1‑148 (MQ=255)
                          tGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTg                                                                                                                   <  1:834487/148‑1 (MQ=255)
                           gTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGt                                                                                                                  >  2:1089871/1‑148 (MQ=255)
                            tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTa                                                                                                                 >  1:54853/1‑148 (MQ=255)
                               ttttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGtt                                                                                                              >  2:1882794/1‑148 (MQ=255)
                                  tGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTAt                                                                                                           <  2:1224109/148‑1 (MQ=255)
                                    tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCt                                                                                                         <  1:1901208/148‑1 (MQ=255)
                                     aGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCtt                                                                                                        >  2:542332/1‑148 (MQ=255)
                                       ttCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTa                                                                                                      <  1:38266/148‑1 (MQ=255)
                                       ttCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTa                                                                                                      <  1:2978917/148‑1 (MQ=255)
                                          cAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                                                   <  2:2501139/148‑1 (MQ=255)
                                               aTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                              >  2:2419588/1‑148 (MQ=255)
                                               aTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                              <  1:1134205/148‑1 (MQ=255)
                                                tAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaaa                                                                                             >  1:581909/1‑148 (MQ=255)
                                                 aaaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAt                                                                                            <  1:2331868/148‑1 (MQ=255)
                                                  caCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAtt                                                                                           <  1:612155/147‑1 (MQ=255)
                                                  aaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAtt                                                                                           >  1:1521444/1‑148 (MQ=255)
                                                  aaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAtt                                                                                           >  2:3186660/1‑148 (MQ=255)
                                                   aCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTa                                                                                          <  1:61452/148‑1 (MQ=255)
                                                   aCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTa                                                                                          >  2:1824274/1‑148 (MQ=255)
                                                    cGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAc                                                                                         >  2:1739520/1‑148 (MQ=255)
                                                      ccccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                       <  1:2071417/148‑1 (MQ=255)
                                                          tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGa                                                                                   >  1:612603/1‑148 (MQ=255)
                                                           gATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                  >  1:2858432/1‑148 (MQ=255)
                                                           gATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                  >  2:2445492/1‑148 (MQ=255)
                                                             tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACa                                                                                >  2:2219550/1‑148 (MQ=255)
                                                             tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACa                                                                                >  1:97410/1‑148 (MQ=255)
                                                             tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACa                                                                                >  1:1650845/1‑148 (MQ=255)
                                                              cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACat                                                                               <  2:638416/148‑1 (MQ=255)
                                                              cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACat                                                                               <  1:3437208/148‑1 (MQ=255)
                                                               gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACata                                                                              <  2:3029526/148‑1 (MQ=255)
                                                               gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACata                                                                              >  1:3499512/1‑148 (MQ=255)
                                                                tcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                             <  2:1093996/148‑1 (MQ=255)
                                                                  ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCg                                                                           <  1:1576486/148‑1 (MQ=255)
                                                                  ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCg                                                                           <  2:2970089/148‑1 (MQ=255)
                                                                    cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                         >  1:880739/1‑148 (MQ=255)
                                                                    cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                         >  1:317315/1‑148 (MQ=255)
                                                                       agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                      <  2:2382813/148‑1 (MQ=255)
                                                                        gaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                                                     >  2:3072745/1‑148 (MQ=255)
                                                                         atatTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCg                                                                    >  2:2638161/1‑148 (MQ=255)
                                                                           atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCt                                                                  >  1:2847289/1‑148 (MQ=255)
                                                                            ttCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCta                                                                 <  2:171800/148‑1 (MQ=255)
                                                                                gCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAcc                                                             <  2:875784/148‑1 (MQ=255)
                                                                                     gcgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGc                                                        <  2:607233/148‑1 (MQ=255)
                                                                                     gcgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGc                                                        <  2:386866/148‑1 (MQ=255)
                                                                                      cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGcc                                                       >  1:1321588/1‑148 (MQ=255)
                                                                                      cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGcc                                                       >  2:341924/1‑148 (MQ=255)
                                                                                       gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCa                                                      <  1:1973615/148‑1 (MQ=255)
                                                                                        cTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                     >  2:1053365/1‑148 (MQ=255)
                                                                                         tAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                    >  1:1530221/1‑148 (MQ=255)
                                                                                         tAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                    <  1:2197790/148‑1 (MQ=255)
                                                                                          aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATc                                                   >  1:3134803/1‑148 (MQ=255)
                                                                                          aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATc                                                   >  1:1379804/1‑148 (MQ=255)
                                                                                            tGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                 <  1:1089871/148‑1 (MQ=255)
                                                                                            tGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                 <  2:2134286/148‑1 (MQ=255)
                                                                                               cAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaa                                              <  2:3234468/148‑1 (MQ=255)
                                                                                                aGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa                                             >  1:2560198/1‑148 (MQ=255)
                                                                                                  aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                           <  2:3141116/148‑1 (MQ=255)
                                                                                                  aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                           >  1:1720574/1‑148 (MQ=255)
                                                                                                  aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                           >  2:3355745/1‑148 (MQ=255)
                                                                                                    ccACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                         >  1:2750675/1‑148 (MQ=255)
                                                                                                    ccACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                         >  1:470111/1‑148 (MQ=255)
                                                                                                         gCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                    >  1:1777016/1‑148 (MQ=255)
                                                                                                           tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATc                                  <  1:520391/148‑1 (MQ=255)
                                                                                                            gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt                                 <  2:1965359/148‑1 (MQ=255)
                                                                                                             aaaTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                >  2:12942/1‑148 (MQ=255)
                                                                                                             aaaTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                <  1:1688369/148‑1 (MQ=255)
                                                                                                               aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                              >  2:2266089/1‑148 (MQ=255)
                                                                                                               aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                              >  1:2248597/1‑148 (MQ=255)
                                                                                                               aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                              >  2:3329190/1‑148 (MQ=255)
                                                                                                                 gAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCt                            <  2:790017/148‑1 (MQ=255)
                                                                                                                 gAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCt                            >  2:1382690/1‑148 (MQ=255)
                                                                                                                 gAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCt                            <  2:1452433/148‑1 (MQ=255)
                                                                                                                  aaTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTa                           >  2:1427447/1‑148 (MQ=255)
                                                                                                                  aaTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTa                           <  2:3343498/148‑1 (MQ=255)
                                                                                                                     tAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                        <  1:3121042/148‑1 (MQ=255)
                                                                                                                     tAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAGATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                        <  1:1037650/148‑1 (MQ=255)
                                                                                                                      aaccaaccATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATggg                       <  1:65422/148‑1 (MQ=255)
                                                                                                                           accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaa                  <  1:285274/148‑1 (MQ=255)
                                                                                                                               tCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa              >  1:211293/1‑148 (MQ=255)
                                                                                                                                ccGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAg             <  1:2764178/148‑1 (MQ=255)
                                                                                                                                 cGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGa            >  2:2042537/1‑148 (MQ=255)
                                                                                                                                   gTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACg          <  1:344859/148‑1 (MQ=255)
                                                                                                                                       cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt      >  1:247216/1‑148 (MQ=255)
                                                                                                                                       cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt      <  1:649302/148‑1 (MQ=255)
                                                                                                                                       cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt      <  1:910816/148‑1 (MQ=255)
                                                                                                                                           cTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTGGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGaa  >  2:3465320/1‑148 (MQ=255)
                                                                                                                                           cTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGaa  <  2:1182537/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAA  >  CP009273/4062280‑4062566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTAG  >  CP009273/4062275‑4062573
                                                                                                                                                     |                                                                                                                                                     
CGGCGCAGCCAGATACGTTTGTGGTTATGGTTGTCGTTTTGTATTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       <  SRR19973065.940910/150‑1 (MQ=60)
CGGCGCAGCCAGATACGTTTGTGGTTAAGGTTGTCGTTTTGTATTCCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       <  SRR19973065.1341204/150‑1 (MQ=60)
CGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       <  SRR19973065.940612/150‑1 (MQ=60)
CGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       <  SRR19973065.1339597/150‑1 (MQ=60)
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       >  SRR19973065.1055488/1‑150 (MQ=60)
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                       <  SRR19973065.1396145/150‑1 (MQ=60)
 GGCGCAGCCAGATACGGTTGTGGTTATGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTA                                                                                                                                                      <  SRR19973065.1947496/150‑1 (MQ=60)
     CAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGC                                                                                                                                                  <  SRR19973065.2871309/150‑1 (MQ=60)
         CAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCG                                                                                                                                              >  SRR19973065.362565/1‑150 (MQ=60)
         CAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATATCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCG                                                                                                                                              >  SRR19973065.3465966/1‑150 (MQ=60)
          AGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGG                                                                                                                                             >  SRR19973065.2278/1‑150 (MQ=60)
            ATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTA                                                                                                                                           <  SRR19973065.2243552/150‑1 (MQ=60)
             TACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAA                                                                                                                                          <  SRR19973065.2002024/150‑1 (MQ=60)
             TACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAA                                                                                                                                          <  SRR19973065.2001966/150‑1 (MQ=60)
                 GTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCATAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATTGCGTCGGTAAGCGC                                                                                                                                      >  SRR19973065.1044568/1‑150 (MQ=60)
                   TGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAA                                                                                                                                    <  SRR19973065.2681288/150‑1 (MQ=60)
                       GTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTT                                                                                                                                >  SRR19973065.1022418/1‑150 (MQ=60)
                       GTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTT                                                                                                                                >  SRR19973065.1190027/1‑150 (MQ=60)
                           AGGTTGTCTTTTTGTATTTCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCC                                                                                                                            <  SRR19973065.3328487/150‑1 (MQ=60)
                            GGTTGTCGTTTTGTATTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCT                                                                                                                           >  SRR19973065.788665/1‑150 (MQ=60)
                            GGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTTACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCT                                                                                                                           >  SRR19973065.409919/1‑150 (MQ=60)
                            GGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCT                                                                                                                           <  SRR19973065.358230/150‑1 (MQ=60)
                               TGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTA                                                                                                                        <  SRR19973065.839931/150‑1 (MQ=60)
                               TGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTA                                                                                                                        >  SRR19973065.55449/1‑150 (MQ=60)
                                    TTTTGTAGTTCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTA                                                                                                                   <  SRR19973065.2697913/150‑1 (MQ=60)
                                         TAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTT                                                                                                              <  SRR19973065.1913659/150‑1 (MQ=60)
                                         TAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTT                                                                                                              <  SRR19973065.1913700/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                             >  SRR19973065.278451/1‑150 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                             <  SRR19973065.539987/150‑1 (MQ=60)
                                           GTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAA                                                                                                            >  SRR19973065.2155820/1‑150 (MQ=60)
                                            TTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAT                                                                                                           <  SRR19973065.2997142/150‑1 (MQ=60)
                                            TTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAT                                                                                                           <  SRR19973065.38733/150‑1 (MQ=60)
                                            TTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAT                                                                                                           <  SRR19973065.38739/150‑1 (MQ=60)
                                            TTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAT                                                                                                           <  SRR19973065.2791556/150‑1 (MQ=60)
                                               CAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCT                                                                                                        <  SRR19973065.613912/150‑1 (MQ=60)
                                                   AATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAA                                                                                                    >  SRR19973065.585541/1‑150 (MQ=60)
                                                    ATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAT                                                                                                   <  SRR19973065.1141557/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                  <  SRR19973065.1706678/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                  >  SRR19973065.1531415/1‑150 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                  >  SRR19973065.1531525/1‑150 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                  >  SRR19973065.555651/1‑150 (MQ=60)
                                                      AAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTA                                                                                                 >  SRR19973065.1686228/1‑150 (MQ=60)
                                                      AAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTA                                                                                                 <  SRR19973065.2346445/150‑1 (MQ=60)
                                                      AAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTA                                                                                                 <  SRR19973065.2346498/150‑1 (MQ=60)
                                                       CACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAC                                                                                                <  SRR19973065.616063/150‑1 (MQ=60)
                                                       AACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAC                                                                                                <  SRR19973065.616061/150‑1 (MQ=60)
                                                       AACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAC                                                                                                <  SRR19973065.616150/150‑1 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                               <  SRR19973065.62203/150‑1 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                               <  SRR19973065.62105/150‑1 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                            <  SRR19973065.2084632/150‑1 (MQ=60)
                                                             CCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGA                                                                                          >  SRR19973065.616515/1‑150 (MQ=60)
                                                             CCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGA                                                                                          >  SRR19973065.616543/1‑150 (MQ=60)
                                                              CTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAA                                                                                         >  SRR19973065.2875989/1‑150 (MQ=60)
                                                                GATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACA                                                                                       >  SRR19973065.2032298/1‑150 (MQ=60)
                                                                GATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACA                                                                                       >  SRR19973065.98310/1‑150 (MQ=60)
                                                                GATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACA                                                                                       >  SRR19973065.1661919/1‑150 (MQ=60)
                                                                  TCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATA                                                                                     >  SRR19973065.3520770/1‑150 (MQ=60)
                                                                   CGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATAT                                                                                    <  SRR19973065.3458177/150‑1 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                >  SRR19973065.319489/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                <  SRR19973065.1641125/150‑1 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                >  SRR19973065.886351/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                >  SRR19973065.1188865/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                >  SRR19973065.1189637/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                <  SRR19973065.1245878/150‑1 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                >  SRR19973065.831329/1‑150 (MQ=60)
                                                                       GGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAT                                                                                <  SRR19973065.1586987/150‑1 (MQ=60)
                                                                         CAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATAT                                                                              <  SRR19973065.1558225/150‑1 (MQ=60)
                                                                              ATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCT                                                                         >  SRR19973065.2864810/1‑150 (MQ=60)
                                                                               TATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTA                                                                        >  SRR19973065.368064/1‑150 (MQ=60)
                                                                                  TCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAC                                                                     >  SRR19973065.3399414/1‑150 (MQ=60)
                                                                                      CGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGA                                                                 >  SRR19973065.264864/1‑150 (MQ=60)
                                                                                      CGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGA                                                                 <  SRR19973065.357132/150‑1 (MQ=60)
                                                                                        TGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGC                                                               <  SRR19973065.3020241/150‑1 (MQ=60)
                                                                                         GGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCC                                                              >  SRR19973065.1330326/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                           >  SRR19973065.2935287/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                           >  SRR19973065.1029211/1‑150 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                           <  SRR19973065.1986468/150‑1 (MQ=60)
                                                                                            GCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAT                                                           >  SRR19973065.1540298/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                          >  SRR19973065.1465980/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                          >  SRR19973065.1388892/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                          >  SRR19973065.3153873/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                          >  SRR19973065.3154273/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                          >  SRR19973065.1388441/1‑150 (MQ=60)
                                                                                              TAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCC                                                         <  SRR19973065.2211866/150‑1 (MQ=60)
                                                                                              TAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCC                                                         >  SRR19973065.794625/1‑150 (MQ=60)
                                                                                              TAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCC                                                         >  SRR19973065.3145960/1‑150 (MQ=60)
                                                                                                ATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAT                                                       <  SRR19973065.2220509/150‑1 (MQ=60)
                                                                                                 TGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATA                                                      <  SRR19973065.1098990/150‑1 (MQ=60)
                                                                                                 TGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATA                                                      <  SRR19973065.1097051/150‑1 (MQ=60)
                                                                                                   TCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAA                                                    >  SRR19973065.2576209/1‑150 (MQ=60)
                                                                                                     AGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACA                                                  >  SRR19973065.1732001/1‑150 (MQ=60)
                                                                                                     AGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACA                                                  >  SRR19973065.924175/1‑150 (MQ=60)
                                                                                                     AGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACA                                                  <  SRR19973065.1485212/150‑1 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                                >  SRR19973065.473333/1‑150 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                                <  SRR19973065.981846/150‑1 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                                >  SRR19973065.2767789/1‑150 (MQ=60)
                                                                                                          CACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAT                                             <  SRR19973065.742763/150‑1 (MQ=60)
                                                                                                           ACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATT                                            <  SRR19973065.1887490/150‑1 (MQ=60)
                                                                                                            CGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTA                                           >  SRR19973065.420355/1‑150 (MQ=60)
                                                                                                            CGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTA                                           >  SRR19973065.1788632/1‑150 (MQ=60)
                                                                                                                TGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTT                                       <  SRR19973065.523640/150‑1 (MQ=60)
                                                                                                                TGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTT                                       <  SRR19973065.523823/150‑1 (MQ=60)
                                                                                                                  AAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTG                                     >  SRR19973065.2262857/1‑150 (MQ=60)
                                                                                                                  AAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTG                                     <  SRR19973065.1699644/150‑1 (MQ=60)
                                                                                                                        ATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGG                               <  SRR19973065.2055743/150‑1 (MQ=60)
                                                                                                                          TAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGT                             >  SRR19973065.904093/1‑150 (MQ=60)
                                                                                                                          TAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGT                             <  SRR19973065.3140052/150‑1 (MQ=60)
                                                                                                                          TAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGAGATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGT                             <  SRR19973065.1044520/150‑1 (MQ=60)
                                                                                                                           AACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTT                            >  SRR19973065.2558702/1‑150 (MQ=60)
                                                                                                                           AACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTT                            <  SRR19973065.66119/150‑1 (MQ=60)
                                                                                                                           AACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTT                            <  SRR19973065.66117/150‑1 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                       <  SRR19973065.287153/150‑1 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                       <  SRR19973065.287151/150‑1 (MQ=60)
                                                                                                                                  CATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAA                     >  SRR19973065.212894/1‑150 (MQ=60)
                                                                                                                                  CATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAA                     >  SRR19973065.648055/1‑150 (MQ=60)
                                                                                                                                     CCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGAC                  <  SRR19973065.2781354/150‑1 (MQ=60)
                                                                                                                                     CCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGAC                  <  SRR19973065.2776131/150‑1 (MQ=60)
                                                                                                                                       GGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGT                >  SRR19973065.2605564/1‑150 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT               <  SRR19973065.347272/150‑1 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT               <  SRR19973065.2126379/150‑1 (MQ=60)
                                                                                                                                          GACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGT             >  SRR19973065.248962/1‑150 (MQ=60)
                                                                                                                                          GACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGT             <  SRR19973065.1749232/150‑1 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT            <  SRR19973065.1968266/150‑1 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTAAACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT            <  SRR19973065.3469962/150‑1 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTG           <  SRR19973065.653576/150‑1 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTG           <  SRR19973065.916738/150‑1 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTG           >  SRR19973065.46782/1‑150 (MQ=60)
                                                                                                                                            CTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTG           <  SRR19973065.2917868/150‑1 (MQ=60)
                                                                                                                                              GGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAA         <  SRR19973065.878306/150‑1 (MQ=60)
                                                                                                                                              GGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAA         <  SRR19973065.1625096/150‑1 (MQ=60)
                                                                                                                                                    CTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA   >  SRR19973065.2495831/1‑150 (MQ=60)
                                                                                                                                                     TATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTAG  <  SRR19973065.1401634/150‑1 (MQ=60)
                                                                                                                                                     TATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTAG  <  SRR19973065.2387900/150‑1 (MQ=60)
                                                                                                                                                     TATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTAG  <  SRR19973065.1171633/150‑1 (MQ=60)
                                                                                                                                                     TATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGGTGAAAATGTAG  >  SRR19973065.1911971/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTAG  >  CP009273/4062275‑4062573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑