Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,062,424 G→T 100% I71I (ATC→ATA)  yihQ ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,062,4240GT100.0% 682.5 / NA 207I71I (ATC→ATA) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (97/110);  total (97/110)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAA  >  CP009273/4062279‑4062567
                                                                                                                                                 |                                                                                                                                               
gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTAt                                                                                                                                               <  1:741239/148‑1 (MQ=255)
  aGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATgg                                                                                                                                             <  2:1981868/148‑1 (MQ=255)
    ccAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCg                                                                                                                                           <  2:2180097/148‑1 (MQ=255)
    ccAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCg                                                                                                                                           <  2:3453825/148‑1 (MQ=255)
      aGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTc                                                                                                                                         >  2:2221834/1‑148 (MQ=255)
      aGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTc                                                                                                                                         >  2:259945/1‑148 (MQ=255)
       gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCg                                                                                                                                        <  2:1438893/148‑1 (MQ=255)
         tACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                      >  1:272769/1‑148 (MQ=255)
               tgtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcgc                                                                                                                                >  2:2819149/1‑148 (MQ=255)
                    ttAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAttt                                                                                                                           >  2:2497372/1‑148 (MQ=255)
                     tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                          >  1:444600/1‑148 (MQ=255)
                     tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                          >  2:2646508/1‑148 (MQ=255)
                        ggTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtctc                                                                                                                       >  1:3519504/1‑148 (MQ=255)
                          ttGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCt                                                                                                                     >  1:733761/1‑148 (MQ=255)
                            gTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGt                                                                                                                   >  1:3265453/1‑148 (MQ=255)
                             tCGTTTTTTAGTTCCACCAAAACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTa                                                                                                                  <  1:1247456/148‑1 (MQ=255)
                             tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTa                                                                                                                  >  2:2508562/1‑148 (MQ=255)
                             tCGTTTTGTAGTTCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTa                                                                                                                  <  1:2733018/148‑1 (MQ=255)
                                  ttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTa                                                                                                             >  2:1622633/1‑148 (MQ=255)
                                    gTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATc                                                                                                           <  2:1785572/148‑1 (MQ=255)
                                     tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCt                                                                                                          >  1:431384/1‑148 (MQ=255)
                                     tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCt                                                                                                          <  1:2276044/148‑1 (MQ=255)
                                     tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCt                                                                                                          <  2:3466994/148‑1 (MQ=255)
                                         tCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTaa                                                                                                      >  2:837942/1‑148 (MQ=255)
                                           cAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                                                    <  2:290318/148‑1 (MQ=255)
                                           cAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                                                    <  2:1336699/148‑1 (MQ=255)
                                             gCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCt                                                                                                  <  2:1635631/148‑1 (MQ=255)
                                               aaTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGa                                                                                                >  1:477082/1‑148 (MQ=255)
                                                aTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                               >  2:648760/1‑148 (MQ=255)
                                                  aaaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAt                                                                                             >  2:706198/1‑148 (MQ=255)
                                                  aaaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAt                                                                                             >  2:3456391/1‑148 (MQ=255)
                                                       ccccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                        <  2:1454555/148‑1 (MQ=255)
                                                       ccccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                        >  2:1711676/1‑148 (MQ=255)
                                                       ccccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                        <  1:3440028/148‑1 (MQ=255)
                                                       ccccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                        <  1:1275398/148‑1 (MQ=255)
                                                        cccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgc                                                                                       >  1:580263/1‑148 (MQ=255)
                                                          cTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCgg                                                                                     <  2:1603642/148‑1 (MQ=255)
                                                           tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGa                                                                                    >  1:369562/1‑148 (MQ=255)
                                                           tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGa                                                                                    >  2:2370837/1‑148 (MQ=255)
                                                             aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGacc                                                                                  >  2:63844/1‑146 (MQ=255)
                                                             aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAAc                                                                                  >  1:664893/1‑148 (MQ=255)
                                                              tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACa                                                                                 <  2:3076684/148‑1 (MQ=255)
                                                               cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACAATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACat                                                                                >  2:2291748/1‑148 (MQ=255)
                                                                gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACata                                                                               <  1:112291/148‑1 (MQ=255)
                                                                   ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCg                                                                            <  2:1882519/148‑1 (MQ=255)
                                                                    gCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGACCATATCGa                                                                           >  1:3038713/1‑148 (MQ=255)
                                                                     cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                          <  2:127168/148‑1 (MQ=255)
                                                                        agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                       <  1:1622633/148‑1 (MQ=255)
                                                                        agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                       >  1:260452/1‑148 (MQ=255)
                                                                        agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                       >  1:367314/1‑148 (MQ=255)
                                                                         gaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                                                      >  2:3433367/1‑148 (MQ=255)
                                                                          atatTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCg                                                                     >  1:3397712/1‑148 (MQ=255)
                                                                            atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCt                                                                   >  1:1831766/1‑148 (MQ=255)
                                                                               cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCtata                                                                <  2:317403/148‑1 (MQ=255)
                                                                               cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCtata                                                                <  2:317073/148‑1 (MQ=255)
                                                                                aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAc                                                               >  2:1577744/1‑148 (MQ=255)
                                                                                aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAc                                                               <  1:989220/148‑1 (MQ=255)
                                                                                    tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGa                                                           >  2:3027238/1‑148 (MQ=255)
                                                                                           aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATc                                                    >  1:3336330/1‑148 (MQ=255)
                                                                                           aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATc                                                    >  1:3295465/1‑148 (MQ=255)
                                                                                            aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATcc                                                   <  1:2221834/148‑1 (MQ=255)
                                                                                            aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATcc                                                   >  2:2421338/1‑148 (MQ=255)
                                                                                                cAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaa                                               <  2:1918302/148‑1 (MQ=255)
                                                                                                  gAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAAc                                             <  1:1383171/148‑1 (MQ=255)
                                                                                                   aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                            >  1:2301518/1‑148 (MQ=255)
                                                                                                   aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                            <  2:1138110/148‑1 (MQ=255)
                                                                                                    aCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                  >  2:423196/1‑141 (MQ=255)
                                                                                                    aCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                  <  1:423208/141‑1 (MQ=255)
                                                                                                    aCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACaa                                           >  2:819975/1‑148 (MQ=255)
                                                                                                     ccACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                          >  2:295367/1‑148 (MQ=255)
                                                                                                     ccACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                          >  1:1636375/1‑148 (MQ=255)
                                                                                                       aCGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGa                                        <  1:1545609/148‑1 (MQ=255)
                                                                                                       aCGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGa                                        <  2:1715886/148‑1 (MQ=255)
                                                                                                        cGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAt                                       >  2:249232/1‑148 (MQ=255)
                                                                                                          gCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                     <  1:1752721/148‑1 (MQ=255)
                                                                                                              aaaTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                 >  1:2359976/1‑148 (MQ=255)
                                                                                                                aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                               <  1:2253715/148‑1 (MQ=255)
                                                                                                                aTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                               <  2:444600/148‑1 (MQ=255)
                                                                                                                 tGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGc                              <  2:2766772/148‑1 (MQ=255)
                                                                                                                    aTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTAt                           <  2:1893485/148‑1 (MQ=255)
                                                                                                                     ttAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATg                          <  2:2138284/148‑1 (MQ=255)
                                                                                                                      tAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                         <  2:3519504/148‑1 (MQ=255)
                                                                                                                         ccaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCTATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGtt                      >  2:1899037/1‑148 (MQ=255)
                                                                                                                         ccaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGtt                      >  1:3112726/1‑148 (MQ=255)
                                                                                                                          caaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTa                     >  2:2868989/1‑148 (MQ=255)
                                                                                                                           aaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaa                    >  2:1878459/1‑148 (MQ=255)
                                                                                                                           aaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaa                    >  2:74020/1‑148 (MQ=255)
                                                                                                                            accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaa                   <  2:1809026/148‑1 (MQ=255)
                                                                                                                            accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaa                   <  1:2508562/148‑1 (MQ=255)
                                                                                                                             ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaaa                  <  2:473495/148‑1 (MQ=255)
                                                                                                                               aTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATa                <  1:2842662/148‑1 (MQ=255)
                                                                                                                                 ccGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAg              >  1:3185091/1‑148 (MQ=255)
                                                                                                                                 ccGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAg              >  2:544487/1‑148 (MQ=255)
                                                                                                                                 ccGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAg              <  1:1818515/148‑1 (MQ=255)
                                                                                                                                     tGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgt          <  1:1065656/148‑1 (MQ=255)
                                                                                                                                      gACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgtt         >  1:1029314/1‑148 (MQ=255)
                                                                                                                                       aCTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGTTTAAAATAAGACgttg        <  2:272870/148‑1 (MQ=255)
                                                                                                                                        cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttgt       >  2:2606517/1‑148 (MQ=255)
                                                                                                                                            cTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGaa   <  1:861658/148‑1 (MQ=255)
                                                                                                                                             tGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGaaa  <  1:1221924/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAA  >  CP009273/4062279‑4062567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA  >  CP009273/4062275‑4062572
                                                                                                                                                     |                                                                                                                                                    
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                      >  SRR19973066.1986473/1‑150 (MQ=60)
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                      <  SRR19973066.1076366/150‑1 (MQ=60)
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACT                                                                                                                                                      >  SRR19973066.1986456/1‑150 (MQ=60)
 GGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTA                                                                                                                                                     <  SRR19973066.3543866/150‑1 (MQ=60)
   CGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATG                                                                                                                                                   <  SRR19973066.2307292/150‑1 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                  <  SRR19973066.746745/150‑1 (MQ=60)
    GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGG                                                                                                                                                  <  SRR19973066.746151/150‑1 (MQ=60)
      AGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCG                                                                                                                                                <  SRR19973066.2992941/150‑1 (MQ=60)
        CCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTC                                                                                                                                              >  SRR19973066.3163548/1‑150 (MQ=60)
           GATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGT                                                                                                                                           >  SRR19973066.274781/1‑150 (MQ=60)
           GATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGT                                                                                                                                           >  SRR19973066.274882/1‑150 (MQ=60)
             TACGTTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAA                                                                                                                                         <  SRR19973066.2582048/150‑1 (MQ=60)
                    GTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAT                                                                                                                                  >  SRR19973066.430099/1‑150 (MQ=60)
                     TGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATT                                                                                                                                 <  SRR19973066.1288585/150‑1 (MQ=60)
                       GTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTT                                                                                                                               >  SRR19973066.448028/1‑150 (MQ=60)
                        TTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTC                                                                                                                              >  SRR19973066.2816861/1‑150 (MQ=60)
                          AAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTC                                                                                                                            >  SRR19973066.3542941/1‑150 (MQ=60)
                            GGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCT                                                                                                                          <  SRR19973066.1496277/150‑1 (MQ=60)
                            GGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCT                                                                                                                          >  SRR19973066.739203/1‑150 (MQ=60)
                              TTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGT                                                                                                                        >  SRR19973066.3286911/1‑150 (MQ=60)
                              TTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGT                                                                                                                        >  SRR19973066.3286934/1‑150 (MQ=60)
                                GTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAG                                                                                                                      >  SRR19973066.806482/1‑150 (MQ=60)
                                 TCGTTTTTTAGTTCCACCAAAACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGT                                                                                                                     <  SRR19973066.1256409/150‑1 (MQ=60)
                                 TCGTTTTGTAGTTCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGT                                                                                                                     <  SRR19973066.2751403/150‑1 (MQ=60)
                                       TGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCT                                                                                                               >  SRR19973066.434725/1‑150 (MQ=60)
                                         TAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTT                                                                                                             <  SRR19973066.2292273/150‑1 (MQ=60)
                                         TAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTT                                                                                                             <  SRR19973066.2291681/150‑1 (MQ=60)
                                          AGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTA                                                                                                            >  SRR19973066.2004723/1‑150 (MQ=60)
                                             TCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATG                                                                                                         <  SRR19973066.1782758/150‑1 (MQ=60)
                                                 GCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGA                                                                                                     >  SRR19973066.480654/1‑150 (MQ=60)
                                                 GCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGA                                                                                                     >  SRR19973066.480681/1‑150 (MQ=60)
                                                    ATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAT                                                                                                  <  SRR19973066.434867/150‑1 (MQ=60)
                                                     TAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATT                                                                                                 <  SRR19973066.3349640/150‑1 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATTGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                              >  SRR19973066.227886/1‑150 (MQ=60)
                                                        ACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACC                                                                                              <  SRR19973066.3442909/150‑1 (MQ=60)
                                                         CGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCG                                                                                             <  SRR19973066.1697055/150‑1 (MQ=60)
                                                          GCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGC                                                                                            >  SRR19973066.584334/1‑150 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                           <  SRR19973066.3463040/150‑1 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                           <  SRR19973066.1284608/150‑1 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                           <  SRR19973066.1284547/150‑1 (MQ=60)
                                                           CCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCG                                                                                           >  SRR19973066.615217/1‑150 (MQ=60)
                                                             CCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGA                                                                                         >  SRR19973066.372458/1‑150 (MQ=60)
                                                               TGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAAC                                                                                       >  SRR19973066.669749/1‑150 (MQ=60)
                                                                   CGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACCTAT                                                                                   >  SRR19973066.920264/1‑150 (MQ=60)
                                                                    GTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATC                                                                                  <  SRR19973066.1057293/150‑1 (MQ=60)
                                                                    GTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATC                                                                                  <  SRR19973066.113434/150‑1 (MQ=60)
                                                                    GTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATC                                                                                  <  SRR19973066.1234966/150‑1 (MQ=60)
                                                                      CGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGACCATATCGA                                                                                >  SRR19973066.3059020/1‑150 (MQ=60)
                                                                      CGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGA                                                                                >  SRR19973066.3059703/1‑150 (MQ=60)
                                                                          AGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATC                                                                            >  SRR19973066.262410/1‑150 (MQ=60)
                                                                          AGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATC                                                                            >  SRR19973066.370197/1‑150 (MQ=60)
                                                                           GAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCC                                                                           >  SRR19973066.370963/1‑150 (MQ=60)
                                                                            AGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCG                                                                          <  SRR19973066.1634393/150‑1 (MQ=60)
                                                                            AGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCG                                                                          >  SRR19973066.3420384/1‑150 (MQ=60)
                                                                              ATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCT                                                                        >  SRR19973066.1844844/1‑150 (MQ=60)
                                                                                    AGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCT                                                                  <  SRR19973066.996519/150‑1 (MQ=60)
                                                                                    AGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCT                                                                  <  SRR19973066.996414/150‑1 (MQ=60)
                                                                                       GTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAG                                                               <  SRR19973066.2664287/150‑1 (MQ=60)
                                                                                        TGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGC                                                              <  SRR19973066.676678/150‑1 (MQ=60)
                                                                                           CGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAA                                                           >  SRR19973066.3366784/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                         >  SRR19973066.3317228/1‑150 (MQ=60)
                                                                                             CTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATC                                                         >  SRR19973066.3358532/1‑150 (MQ=60)
                                                                                                ATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAT                                                      >  SRR19973066.1583797/1‑150 (MQ=60)
                                                                                                ATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAT                                                      <  SRR19973066.2237201/150‑1 (MQ=60)
                                                                                                 gatctGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATA                                                     <  SRR19973066.426494/145‑1 (MQ=60)
                                                                                                 gatctGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATA                                                     <  SRR19973066.426482/145‑1 (MQ=60)
                                                                                                     AGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACA                                                 >  SRR19973066.2317282/1‑150 (MQ=60)
                                                                                                      GAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAA                                                <  SRR19973066.1393169/150‑1 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                               >  SRR19973066.1648270/1‑150 (MQ=60)
                                                                                                       AACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAG                                               >  SRR19973066.1648205/1‑150 (MQ=60)
                                                                                                        ACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGG                                              <  SRR19973066.3365624/150‑1 (MQ=60)
                                                                                                           ACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATT                                           <  SRR19973066.1556667/150‑1 (MQ=60)
                                                                                                            CGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTA                                          <  SRR19973066.1218115/150‑1 (MQ=60)
                                                                                                            CGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACAGCGGAACATATCGATATCCGATATACCTGAGCCAATCCATAAACAAGGATTA                                          <  SRR19973066.359022/150‑1 (MQ=60)
                                                                                                              GCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATC                                        >  SRR19973066.1100825/1‑150 (MQ=60)
                                                                                                              GCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATC                                        >  SRR19973066.3226508/1‑150 (MQ=60)
                                                                                                              GCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATC                                        <  SRR19973066.1765326/150‑1 (MQ=60)
                                                                                                               CTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCT                                       <  SRR19973066.2467738/150‑1 (MQ=60)
                                                                                                                TGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTT                                      >  SRR19973066.2375976/1‑150 (MQ=60)
                                                                                                                TGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTT                                      <  SRR19973066.566083/150‑1 (MQ=60)
                                                                                                                    ATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCT                                  <  SRR19973066.2269250/150‑1 (MQ=60)
                                                                                                                           AACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTT                           >  SRR19973066.3133514/1‑150 (MQ=60)
                                                                                                                                ACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAT                      <  SRR19973066.2525640/150‑1 (MQ=60)
                                                                                                                                   ATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAG                   >  SRR19973066.2254264/1‑150 (MQ=60)
                                                                                                                                   ATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAG                   <  SRR19973066.2861764/150‑1 (MQ=60)
                                                                                                                                   ATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAG                   >  SRR19973066.3206279/1‑150 (MQ=60)
                                                                                                                                    TCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGA                  >  SRR19973066.2352157/1‑150 (MQ=60)
                                                                                                                                     CCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGAC                 <  SRR19973066.1831472/150‑1 (MQ=60)
                                                                                                                                     CCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGAC                 <  SRR19973066.1831571/150‑1 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT              >  SRR19973066.2274073/1‑150 (MQ=60)
                                                                                                                                        GTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTT              >  SRR19973066.1036894/1‑150 (MQ=60)
                                                                                                                                         TGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTG             <  SRR19973066.1073469/150‑1 (MQ=60)
                                                                                                                                           ACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTT           <  SRR19973066.3473398/150‑1 (MQ=60)
                                                                                                                                                CTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAA      <  SRR19973066.867859/150‑1 (MQ=60)
                                                                                                                                                 TGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAAT     <  SRR19973066.1230555/150‑1 (MQ=60)
                                                                                                                                                    CTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA  >  SRR19973066.2323711/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGGCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA  >  CP009273/4062275‑4062572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑