Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F86 I0 R1	593	297.4	9515568	94.4%	8982696	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,144,158	C→A	55.3%	intergenic (+253/+19)	yghA → / ← exbD	putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,144,158	0	C	A	55.3%	38.1 / inf	257	intergenic (+253/+19)	yghA/exbD	putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand): ref base C (72/43); new base A (90/52); total (162/95)
Fisher's exact test for biased strand distribution p-value = 8.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144009‑3144306

aTGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAgc                                                                                                                                                      >  2:3904790/1‑150 (MQ=255)

       aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                               <  1:2509627/150‑1 (MQ=255)

       aaTAGATTTGGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAAGGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                               <  2:4433058/150‑1 (MQ=255)

        aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCACTAGATTTTGTAGGCCTGAGGAGCGGAGCGCATCAgg                                                                                                                                              >  2:4747110/1‑150 (MQ=255)

        aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAgg                                                                                                                                              >  1:3222664/1‑150 (MQ=255)

        aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAgg                                                                                                                                              <  2:1664085/150‑1 (MQ=255)

        aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                              >  2:875800/1‑150 (MQ=255)

         tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGc                                                                                                                                             >  2:2613747/1‑150 (MQ=255)

             ttttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAAtt                                                                                                                                         >  1:4168409/1‑150 (MQ=255)

               gtGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCt                                                                                                                                       <  1:4272637/149‑1 (MQ=255)

                tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCta                                                                                                                                      >  1:4461146/1‑150 (MQ=255)

                tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCta                                                                                                                                      >  1:2296027/1‑150 (MQ=255)

                   aGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactt                                                                                                                                   >  1:3628821/1‑150 (MQ=255)

                    ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttt                                                                                                                                  >  1:3144139/1‑150 (MQ=255)

                      ccTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCtactttac                                                                                                                                >  1:2088136/1‑150 (MQ=255)

                       cTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttact                                                                                                                               >  1:356242/1‑150 (MQ=255)

                        tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCtactttactt                                                                                                                              >  2:2461877/1‑150 (MQ=255)

                           tAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGc                                                                                                                           >  1:787659/1‑150 (MQ=255)

                               cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTg                                                                                                                       >  1:595624/1‑150 (MQ=255)

                                gTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTgg                                                                                                                      >  1:3357412/1‑150 (MQ=255)

                                gTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCATTATCCGGCCTACAAACGCACTAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTgg                                                                                                                      <  1:4055590/150‑1 (MQ=255)

                                  aGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCg                                                                                                                    >  1:259459/1‑150 (MQ=255)

                                     gcATCAGGCATTGCGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGtt                                                                                                                 <  2:573139/150‑1 (MQ=255)

                                     gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGtt                                                                                                                 >  2:4262825/1‑150 (MQ=255)

                                     gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGtt                                                                                                                 >  1:1662007/1‑150 (MQ=255)

                                     gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGtt                                                                                                                 >  2:3809839/1‑150 (MQ=255)

                                       aTCAGGCATTGAGCACCTATTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                               >  2:1913809/1‑150 (MQ=255)

                                       aTCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                               <  1:4125306/150‑1 (MQ=255)

                                       aTCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                               >  2:2819570/1‑150 (MQ=255)

                                         cAGGCATTGAGCACCTACTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                             <  2:3219969/150‑1 (MQ=255)

                                         cAGGCATTGAGCACCTAAGGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                             <  2:2502518/150‑1 (MQ=255)

                                          aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttcttc                                                                                                            <  1:2818587/150‑1 (MQ=255)

                                                tGAGCCCCTAATGCCGGATGCTTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAGTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGAc                                                                                                      <  2:3471923/150‑1 (MQ=255)

                                                 gAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGAcc                                                                                                     >  2:4208570/1‑150 (MQ=255)

                                                 gAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATGCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCCATTCTCCTTTACTTCGCGTTGGCGGTTTCTTCGCCGAcc                                                                                                     >  2:4208774/1‑150 (MQ=255)

                                                      ccTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                                >  1:834562/1‑150 (MQ=255)

                                                      ccTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                                >  1:193711/1‑150 (MQ=255)

                                                       cTACTGCCGGAGGCGGCGTGACCGCCTTATCCGGCCTACAACCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                               <  1:4580397/150‑1 (MQ=25)

                                                       cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                               >  1:435358/1‑150 (MQ=255)

                                                       cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                               >  2:555265/1‑150 (MQ=255)

                                                          aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATc                                                                                            <  1:2699264/150‑1 (MQ=255)

                                                               ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAg                                                                                       >  1:3407226/1‑150 (MQ=255)

                                                                 aTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGt                                                                                     >  2:1119351/1‑150 (MQ=255)

                                                                     gtcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAcc                                                                                 >  2:1609645/1‑150 (MQ=255)

                                                                          gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTCGCGCTTCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCt                                                                            <  2:3971377/150‑1 (MQ=255)

                                                                          gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCt                                                                            <  2:4281727/150‑1 (MQ=255)

                                                                            aCGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGa                                                                          >  1:1342673/1‑150 (MQ=255)

                                                                             cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAt                                                                         <  2:2177493/150‑1 (MQ=255)

                                                                             cGCCTTATCCGGCCTACAAACGAACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAt                                                                         <  1:1980198/150‑1 (MQ=255)

                                                                             cGCCCTAGCCGGCCCCCCAACGCACGCAGAGTCACCCTGTAGCAATAGATCTTGAAGGCCAGATGAGCGTCGCGCCTCAGGCAATCCTACTTTACGACGCAATGGCGGATTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCGGAt                                                                         <  2:4689960/150‑1 (MQ=255)

                                                                               ccTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                       >  2:2305025/1‑150 (MQ=255)

                                                                               ccTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                       >  2:3293756/1‑150 (MQ=255)

                                                                                 ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAg                                                                     >  2:1498796/1‑150 (MQ=255)

                                                                                 ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAg                                                                     >  1:684347/1‑150 (MQ=255)

                                                                                     ccGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTa                                                                 <  1:1562986/150‑1 (MQ=255)

                                                                                        gCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATcc                                                              <  1:1737324/150‑1 (MQ=255)

                                                                                          cTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                            >  2:1534873/1‑150 (MQ=255)

                                                                                           tACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                           >  2:1856508/1‑150 (MQ=255)

                                                                                            aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTa                                                          >  2:299284/1‑150 (MQ=255)

                                                                                            aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTa                                                          <  1:3418626/150‑1 (MQ=255)

                                                                                             cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAc                                                         >  2:2419211/1‑150 (MQ=255)

                                                                                               aaCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                       >  1:2627033/1‑150 (MQ=255)

                                                                                                aCGCACGAAGATTCAACCTGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCtt                                                      <  1:1096459/150‑1 (MQ=255)

                                                                                                      gAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCaa                                                >  1:3904968/1‑150 (MQ=255)

                                                                                                       aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                               <  1:103064/150‑1 (MQ=255)

                                                                                                        aGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                              >  2:3463205/1‑150 (MQ=255)

                                                                                                        aGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                              <  2:804222/150‑1 (MQ=255)

                                                                                                         gATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                             >  2:2352941/1‑150 (MQ=255)

                                                                                                             cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGAAGCGCAACAGGCAATTCAACATTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCg                                         <  1:1847728/150‑1 (MQ=255)

                                                                                                                 aTGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                     <  2:496546/150‑1 (MQ=255)

                                                                                                                  tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATc                                    <  2:3081294/150‑1 (MQ=255)

                                                                                                                   gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                                   <  1:1993021/150‑1 (MQ=255)

                                                                                                                   gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                                   <  2:4257847/150‑1 (MQ=255)

                                                                                                                    ttGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                                  >  1:4007306/1‑150 (MQ=255)

                                                                                                                     tGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGAc                                 >  2:4252095/1‑150 (MQ=255)

                                                                                                                       cAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                               <  2:375350/150‑1 (MQ=255)

                                                                                                                         aTAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCGTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtt                             >  2:3258998/1‑150 (MQ=255)

                                                                                                                         aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtt                             <  2:3948110/150‑1 (MQ=255)

                                                                                                                          tAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                            >  2:1627833/1‑150 (MQ=255)

                                                                                                                          tAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                            >  2:1648047/1‑150 (MQ=255)

                                                                                                                           aGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                           >  1:1694145/1‑150 (MQ=255)

                                                                                                                              ttttGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACGTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                        <  2:2108334/150‑1 (MQ=255)

                                                                                                                                   tAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTc                   >  2:219680/1‑150 (MQ=255)

                                                                                                                                   tAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTc                   >  1:4053520/1‑150 (MQ=255)

                                                                                                                                   tAGGCCTGATGAGCGTAGAGCATCAGGCAATTCGACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTc                   <  2:1294842/150‑1 (MQ=255)

                                                                                                                                    aGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCg                  >  1:2917552/1‑150 (MQ=255)

                                                                                                                                       ccTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaa               >  1:2965477/1‑150 (MQ=255)

                                                                                                                                        cTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaag              >  1:2665410/1‑150 (MQ=255)

                                                                                                                                         tgatgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga             >  1:460501/1‑150 (MQ=255)

                                                                                                                                         tgatgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga             >  2:910256/1‑150 (MQ=255)

                                                                                                                                           atgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag           >  1:3748208/1‑150 (MQ=255)

                                                                                                                                           atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag           <  2:2736583/150‑1 (MQ=255)

                                                                                                                                             gaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAt         >  1:3506579/1‑150 (MQ=255)

                                                                                                                                              aGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg        >  1:4737448/1‑150 (MQ=255)

                                                                                                                                                cGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt      >  2:2286372/1‑150 (MQ=255)

                                                                                                                                                cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt      >  2:3623525/1‑150 (MQ=255)

                                                                                                                                                cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt      <  1:2284518/150‑1 (MQ=255)

                                                                                                                                                cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt      >  1:1413097/1‑150 (MQ=255)

                                                                                                                                                   aGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATggtggt   <  2:890082/150‑1 (MQ=255)

                                                                                                                                                    gcgcATCAGGCAATTCTACTGTACTTCGCTTTGACGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGgtggtg  <  1:4487184/150‑1 (MQ=255)

ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144009‑3144306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144010‑3144306

TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGAGTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCG                                                                                                                                                     >  SRR19973082.631297/1‑150 (MQ=60)

   TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCAT                                                                                                                                                  >  SRR19973082.2227657/1‑150 (MQ=60)

    GCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATC                                                                                                                                                 <  SRR19973082.1737353/150‑1 (MQ=60)

     CAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCA                                                                                                                                                <  SRR19973082.4458456/150‑1 (MQ=60)

      AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                               <  SRR19973082.4730081/150‑1 (MQ=60)

      AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                               <  SRR19973082.2525289/150‑1 (MQ=60)

      AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                               <  SRR19973082.2525166/150‑1 (MQ=60)

       ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGG                                                                                                                                              >  SRR19973082.3242385/1‑150 (MQ=60)

            TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATT                                                                                                                                         >  SRR19973082.1797041/1‑150 (MQ=60)

            TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATT                                                                                                                                         >  SRR19973082.4193922/1‑150 (MQ=60)

            TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATT                                                                                                                                         >  SRR19973082.3125301/1‑150 (MQ=60)

              GTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCT                                                                                                                                       <  SRR19973082.4298563/150‑1 (MQ=60)

               TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                      >  SRR19973082.4488245/1‑150 (MQ=60)

               TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                      >  SRR19973082.4492723/1‑150 (MQ=60)

               TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                      >  SRR19973082.2310328/1‑150 (MQ=60)

                GTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTAC                                                                                                                                     >  SRR19973082.3643615/1‑150 (MQ=60)

                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACT                                                                                                                                    >  SRR19973082.1642216/1‑150 (MQ=60)

                  AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGTTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTT                                                                                                                                   <  SRR19973082.1952614/150‑1 (MQ=60)

                  AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTT                                                                                                                                   >  SRR19973082.3651119/1‑150 (MQ=60)

                   GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                  >  SRR19973082.3163220/1‑150 (MQ=60)

                   GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                  >  SRR19973082.3163155/1‑150 (MQ=60)

                    GCGTGATAAGCGTAGCGCATCTGGATTGGAGCACCTACTGCGGGTTGCGTGGTGAACGCCTTATCCGGCCTACAAACGCACTAAGATTCAACTTGTTGAAATTGTTTTTGTTGGCCTGATGTGCGTTGCGCTTCAGGCAATTCTACTTTA                                                                                                                                 <  SRR19973082.3263629/150‑1 (MQ=47)

                    GCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTA                                                                                                                                 <  SRR19973082.2823309/150‑1 (MQ=60)

                    GCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTA                                                                                                                                 <  SRR19973082.2823264/150‑1 (MQ=60)

                     CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTAC                                                                                                                                >  SRR19973082.2101428/1‑150 (MQ=60)

                      CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTAGTTTACT                                                                                                                               >  SRR19973082.3405232/1‑150 (MQ=60)

                      CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                               >  SRR19973082.358576/1‑150 (MQ=60)

                       TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                              >  SRR19973082.1674606/1‑150 (MQ=60)

                          TAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGC                                                                                                                           >  SRR19973082.792692/1‑150 (MQ=60)

                          TAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGC                                                                                                                           >  SRR19973082.792636/1‑150 (MQ=60)

                           AAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCT                                                                                                                          >  SRR19973082.2666185/1‑150 (MQ=60)

                           AAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCT                                                                                                                          >  SRR19973082.2665391/1‑150 (MQ=60)

                              CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                       >  SRR19973082.4124097/1‑150 (MQ=60)

                              CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                       >  SRR19973082.599860/1‑150 (MQ=60)

                               GTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGG                                                                                                                      >  SRR19973082.3378167/1‑150 (MQ=60)

                               GTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCATTATCCGGCCTACAAACGCACTAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGG                                                                                                                      <  SRR19973082.4080461/150‑1 (MQ=60)

                               GTAGCGAATCTGGCATTGAGCACCTAATGCCGTTTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGTAGATTCAACTTGTTGCATTTTTTTTTGTTGGCCTGTTGTGCGTTGCGCATCAGGCAATTCTACTTTACTTCGCTTTGG                                                                                                                      <  SRR19973082.2611260/150‑1 (MQ=60)

                                 AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                    >  SRR19973082.3623972/1‑150 (MQ=60)

                                 AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                    >  SRR19973082.261420/1‑150 (MQ=60)

                                 AGCGCATCAGGCATTGAGCACCTAATGCAGGATGCGTCGTGAACGCCTTATCCGGCATACAAACGCACGAAGATTCAACATGTTGCAATATATTTTGTATGCCTGATGAGCGTATAGCATCAGGCAATTCTAATTTACTTCGCTTTGGCG                                                                                                                    >  SRR19973082.260297/1‑150 (MQ=60)

                                    GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 >  SRR19973082.1673073/1‑150 (MQ=60)

                                     CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTT                                                                                                                >  SRR19973082.4670467/1‑150 (MQ=60)

                                      ATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTC                                                                                                               >  SRR19973082.4320091/1‑150 (MQ=60)

                                      ATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTC                                                                                                               <  SRR19973082.4150575/150‑1 (MQ=60)

                                      ATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTC                                                                                                               >  SRR19973082.1923639/1‑150 (MQ=60)

                                         AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                            >  SRR19973082.2048776/1‑150 (MQ=60)

                                         AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                            <  SRR19973082.2835839/150‑1 (MQ=60)

                                         AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                            >  SRR19973082.2276004/1‑150 (MQ=60)

                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                        <  SRR19973082.626617/150‑1 (MQ=60)

                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                        >  SRR19973082.4187980/1‑150 (MQ=60)

                                                 AGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCA                                                                                                    >  SRR19973082.3704082/1‑150 (MQ=60)

                                                     CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                                >  SRR19973082.840114/1‑150 (MQ=60)

                                                     CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                                >  SRR19973082.3859024/1‑150 (MQ=60)

                                                     CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                                >  SRR19973082.840102/1‑150 (MQ=60)

                                                     CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                                >  SRR19973082.195341/1‑150 (MQ=60)

                                                      CTACTGCCGGAGGCGGCGTGACCGCCTTATCCGGCCTACAACCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               <  SRR19973082.4608204/150‑1 (MQ=60)

                                                      CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               >  SRR19973082.438330/1‑150 (MQ=60)

                                                      CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               >  SRR19973082.4478609/1‑150 (MQ=60)

                                                        AATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAAATCTACTTTACTTCGCTTTGTCGGTTTCTTCGCCGACCAGACCTAT                                                                                             >  SRR19973082.2045995/1‑150 (MQ=60)

                                                        AATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTAT                                                                                             >  SRR19973082.3581514/1‑150 (MQ=60)

                                                         ATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCTCCGACCAGACCTATA                                                                                            >  SRR19973082.500083/1‑150 (MQ=60)

                                                         ATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATC                                                                                            <  SRR19973082.2715781/150‑1 (MQ=60)

                                                          TGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCT                                                                                           >  SRR19973082.4681995/1‑150 (MQ=60)

                                                            CCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTC                                                                                         >  SRR19973082.2632285/1‑150 (MQ=60)

                                                              GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                       >  SRR19973082.3428385/1‑150 (MQ=60)

                                                              GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                       >  SRR19973082.3428261/1‑150 (MQ=60)

                                                              GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                       <  SRR19973082.3523489/150‑1 (MQ=60)

                                                                    TTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTTGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACC                                                                                 <  SRR19973082.1990339/150‑1 (MQ=60)

                                                                          AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                           <  SRR19973082.151295/150‑1 (MQ=60)

                                                                           ACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGA                                                                          >  SRR19973082.1351921/1‑150 (MQ=60)

                                                                           ACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGA                                                                          >  SRR19973082.1351884/1‑150 (MQ=60)

                                                                            CGCCTTATCCGGCCTACAAACGAACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                         <  SRR19973082.1992591/150‑1 (MQ=60)

                                                                             GCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATG                                                                        <  SRR19973082.3711903/150‑1 (MQ=60)

                                                                                TTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAG                                                                     >  SRR19973082.689009/1‑150 (MQ=60)

                                                                                    CCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTA                                                                 <  SRR19973082.1573197/150‑1 (MQ=60)

                                                                                     CGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTAT                                                                <  SRR19973082.4350892/150‑1 (MQ=60)

                                                                                      GGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATC                                                               <  SRR19973082.4521595/150‑1 (MQ=60)

                                                                                       GCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCC                                                              <  SRR19973082.1748849/150‑1 (MQ=60)

                                                                                           ACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTA                                                          <  SRR19973082.1041507/150‑1 (MQ=60)

                                                                                           ACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTA                                                          <  SRR19973082.3439714/150‑1 (MQ=60)

                                                                                            CAAACGCACGAAGATTCAACATGTGGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAC                                                         <  SRR19973082.1666405/150‑1 (MQ=60)

                                                                                              AACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCT                                                       >  SRR19973082.2643297/1‑150 (MQ=60)

                                                                                               ACGCACGAAGATTCAACCTGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTT                                                      <  SRR19973082.1103885/150‑1 (MQ=60)

                                                                                                     GAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAA                                                >  SRR19973082.3928832/1‑150 (MQ=60)

                                                                                                      AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                               <  SRR19973082.104069/150‑1 (MQ=60)

                                                                                                            CAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGAAGCGCAACAGGCAATTCAACATTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCG                                         <  SRR19973082.1859739/150‑1 (MQ=60)

                                                                                                             AACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGT                                        >  SRR19973082.1992651/1‑150 (MQ=60)

                                                                                                              ACATGTTGCAATAGATTTTGTTGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTA                                       <  SRR19973082.1954291/150‑1 (MQ=60)

                                                                                                                ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                     <  SRR19973082.4230832/150‑1 (MQ=60)

                                                                                                                  GTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCG                                   <  SRR19973082.2005547/150‑1 (MQ=60)

                                                                                                                   TTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGA                                  >  SRR19973082.4031787/1‑150 (MQ=60)

                                                                                                                   TTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGA                                  >  SRR19973082.4031788/1‑150 (MQ=60)

                                                                                                                    TGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGAC                                 >  SRR19973082.3396564/1‑150 (MQ=60)

                                                                                                                     GCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACG                                >  SRR19973082.1414834/1‑150 (MQ=60)

                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCTGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCTGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                           <  SRR19973082.4288722/150‑1 (MQ=60)

                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                           <  SRR19973082.3972879/150‑1 (MQ=60)

                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                           >  SRR19973082.1705409/1‑150 (MQ=60)

                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                           <  SRR19973082.3973861/150‑1 (MQ=60)

                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGACGACCAGACCTATCTTCAGGTCACCCGCCTGATGCAGCGTATCCATTACCTTCATCCACGTCTCGGTATCGACGGTTTT                           >  SRR19973082.4105431/1‑150 (MQ=60)

                                                                                                                            ATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGT                         >  SRR19973082.716103/1‑150 (MQ=60)

                                                                                                                            ATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGT                         >  SRR19973082.1547312/1‑150 (MQ=60)

                                                                                                                            ATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGT                         >  SRR19973082.4036854/1‑150 (MQ=60)

                                                                                                                             TTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTC                        >  SRR19973082.2390954/1‑150 (MQ=60)

                                                                                                                               TTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCG                      >  SRR19973082.2869661/1‑150 (MQ=60)

                                                                                                                                TGTAGGCCTGATGAGCGTAGCTCATCAGGCAATTCTACTTTACTTCGCTTAGGCGGTTACTTCTCCGAACAGACATATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATAAACTTCACGTAAACGAAGGTTTTGTaagc                     >  SRR19973082.4570647/1‑146 (MQ=60)

                                                                                                                                  TAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTC                   >  SRR19973082.4078376/1‑150 (MQ=60)

                                                                                                                                  TAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTC                   >  SRR19973082.4078368/1‑150 (MQ=60)

                                                                                                                                   AGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCG                  >  SRR19973082.2935587/1‑150 (MQ=60)

                                                                                                                                   AGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCG                  >  SRR19973082.2935598/1‑150 (MQ=60)

                                                                                                                                    GGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGG                 <  SRR19973082.2988125/150‑1 (MQ=60)

                                                                                                                                      CCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAA               >  SRR19973082.2983793/1‑150 (MQ=60)

                                                                                                                                       CTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG              <  SRR19973082.1877244/150‑1 (MQ=60)

                                                                                                                                       CTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG              <  SRR19973082.1717786/150‑1 (MQ=60)

                                                                                                                                       CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG              >  SRR19973082.2681819/1‑150 (MQ=60)

                                                                                                                                       CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG              >  SRR19973082.2682475/1‑150 (MQ=60)

                                                                                                                                        TGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGA             >  SRR19973082.463725/1‑150 (MQ=60)

                                                                                                                                        TGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGA             >  SRR19973082.463692/1‑150 (MQ=60)

                                                                                                                                          ATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG           >  SRR19973082.3771379/1‑150 (MQ=60)

                                                                                                                                            GAGCGTAGAGCATCAGGCTATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAT         <  SRR19973082.1171325/150‑1 (MQ=60)

                                                                                                                                            GAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAT         >  SRR19973082.3526367/1‑150 (MQ=60)

                                                                                                                                            GAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAT         >  SRR19973082.3527996/1‑150 (MQ=60)

                                                                                                                                             AGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATG        >  SRR19973082.4765892/1‑150 (MQ=60)

                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      >  SRR19973082.1422753/1‑150 (MQ=60)

                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      <  SRR19973082.3337652/150‑1 (MQ=60)

                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      <  SRR19973082.2298795/150‑1 (MQ=60)

                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      <  SRR19973082.2298783/150‑1 (MQ=60)

                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG     >  SRR19973082.2860848/1‑150 (MQ=60)

                                                                                                                                                  AGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT   <  SRR19973082.2564452/150‑1 (MQ=60)

                                                                                                                                                   GCGCATCAGGCAATTCTACTTTACTTCGCTTTGACGGTTTCTTCGCCGACCAGACCTATCTTCAGGTACCCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  <  SRR19973082.4514434/150‑1 (MQ=60)

                                                                                                                                                   GCGCATCAGGCAATTCTACTGTACTTCGCTTTGACGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  <  SRR19973082.4514445/150‑1 (MQ=60)

TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144010‑3144306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`