Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,144,158 C→A 100% intergenic (+253/+19) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,144,1580CA100.0% 737.0 / NA 219intergenic (+253/+19)yghA/exbDputative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (123/96);  total (123/96)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302
                                                                                                                                                 |                                                                                                                                                
 gCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCa                                                                                                                                               <  2:1635431/148‑1 (MQ=255)
 gCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCa                                                                                                                                               <  2:117688/148‑1 (MQ=255)
  cAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCAt                                                                                                                                              >  2:658282/1‑148 (MQ=255)
   aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             <  2:51423/148‑1 (MQ=255)
   aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             <  2:4527/148‑1 (MQ=255)
   aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             >  2:1843600/1‑148 (MQ=255)
     tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           >  1:1103612/1‑148 (MQ=255)
      aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          >  2:2862298/1‑148 (MQ=255)
      aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          >  1:268564/1‑148 (MQ=255)
         ttttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCaa                                                                                                                                       >  1:2371317/1‑148 (MQ=255)
          tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAt                                                                                                                                      >  2:696195/1‑148 (MQ=255)
            tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTc                                                                                                                                    <  1:914946/148‑1 (MQ=255)
               aGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtac                                                                                                                                 <  1:3333860/148‑1 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                >  1:1525489/1‑148 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                <  1:683641/148‑1 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                >  2:1795817/1‑148 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                <  1:2115820/148‑1 (MQ=255)
                  ccTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttt                                                                                                                              <  2:1064274/148‑1 (MQ=255)
                  ccTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttt                                                                                                                              <  2:1868766/148‑1 (MQ=255)
                      aTAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttactt                                                                                                                          <  1:995989/148‑1 (MQ=255)
                       tAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTc                                                                                                                         >  1:192939/1‑148 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     >  1:806948/1‑148 (MQ=255)
                                 gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCgg                                                                                                               <  1:771224/148‑1 (MQ=255)
                                 gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCgg                                                                                                               <  1:592259/148‑1 (MQ=255)
                                 gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCgg                                                                                                               <  1:437025/148‑1 (MQ=255)
                                   aTCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGtt                                                                                                             >  1:1669293/1‑148 (MQ=255)
                                    tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGttt                                                                                                            <  1:2643313/148‑1 (MQ=255)
                                    tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGttt                                                                                                            <  1:3144864/148‑1 (MQ=255)
                                    tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGACTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGttt                                                                                                            >  2:1372898/1‑148 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  1:2062101/1‑148 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  2:1526987/1‑148 (MQ=255)
                                      aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttct                                                                                                          <  1:593420/148‑1 (MQ=255)
                                       ggCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                         >  1:3079726/1‑148 (MQ=255)
                                       ggCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                         >  1:3079887/1‑148 (MQ=255)
                                           ttGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGcc                                                                                                     >  2:2984333/1‑148 (MQ=255)
                                            tGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCg                                                                                                    >  1:2132297/1‑148 (MQ=255)
                                              aGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGAc                                                                                                  <  2:2655764/148‑1 (MQ=255)
                                                  ccTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGa                                                                                              >  1:1164926/1‑148 (MQ=255)
                                                   cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAc                                                                                             <  1:1203200/148‑1 (MQ=255)
                                                      aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTa                                                                                          <  2:2737605/148‑1 (MQ=255)
                                                         ccGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCt                                                                                       <  1:2339073/148‑1 (MQ=255)
                                                           ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTc                                                                                     >  2:2689006/1‑148 (MQ=255)
                                                           ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTc                                                                                     <  2:795690/148‑1 (MQ=255)
                                                             aTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAg                                                                                   <  1:3498583/148‑1 (MQ=255)
                                                              tGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAgg                                                                                  >  2:1339569/1‑148 (MQ=255)
                                                                cgtcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTa                                                                                >  1:1153622/1‑148 (MQ=255)
                                                                  tcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCACCATGTTGCAATAGTTTTTTTTGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAc                                                                              <  2:3425796/148‑1 (MQ=38)
                                                                     tGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCg                                                                           <  1:2962061/148‑1 (MQ=255)
                                                                      gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGc                                                                          <  1:2097665/148‑1 (MQ=255)
                                                                      gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGc                                                                          <  2:2440327/148‑1 (MQ=255)
                                                                       aaCGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGcc                                                                         >  2:2169302/1‑148 (MQ=255)
                                                                         cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       >  1:2427466/1‑148 (MQ=255)
                                                                         cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       >  2:497444/1‑148 (MQ=255)
                                                                            cTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATg                                                                    >  1:2072887/1‑148 (MQ=255)
                                                                                tCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGc                                                                >  1:491927/1‑148 (MQ=255)
                                                                                tCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGc                                                                >  1:491908/1‑148 (MQ=255)
                                                                                     ccTACAAACGCACGAAGATTCAACATGTTGCAAGAGATATTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATc                                                           <  1:1657436/148‑1 (MQ=255)
                                                                                           aaCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAc                                                     <  1:2862298/148‑1 (MQ=255)
                                                                                             cGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                   <  1:1745695/148‑1 (MQ=255)
                                                                                             cGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                   <  1:2570904/148‑1 (MQ=255)
                                                                                             cGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                   >  1:1084906/1‑148 (MQ=255)
                                                                                               cACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATACTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtc                                                 >  2:634151/1‑148 (MQ=255)
                                                                                                   aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatca                                             >  1:2706923/1‑148 (MQ=255)
                                                                                                     gATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                           >  2:1634001/1‑148 (MQ=255)
                                                                                                     gATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                           >  2:305133/1‑148 (MQ=255)
                                                                                                      aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  1:93465/1‑148 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         <  1:689793/148‑1 (MQ=255)
                                                                                                        tCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                        >  2:1687638/1‑148 (MQ=255)
                                                                                                         cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                       >  1:609103/1‑148 (MQ=255)
                                                                                                         cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                       >  1:1144609/1‑148 (MQ=255)
                                                                                                           aCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCg                                     >  2:2487364/1‑148 (MQ=255)
                                                                                                              tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTaa                                  >  2:3425447/1‑148 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 <  2:3130635/148‑1 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 >  1:1352005/1‑148 (MQ=255)
                                                                                                                ttGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATc                                >  1:3426050/1‑148 (MQ=255)
                                                                                                                 tGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               >  2:2362899/1‑148 (MQ=255)
                                                                                                                   cAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGAc                             <  2:2528351/148‑1 (MQ=255)
                                                                                                                     aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           >  2:1619248/1‑148 (MQ=255)
                                                                                                                      tAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCGTCATCAACGTCTCGTACTCGACGGt                          >  1:1832455/1‑148 (MQ=255)
                                                                                                                       aGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtt                         >  2:1940142/1‑148 (MQ=255)
                                                                                                                        gATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                        <  2:783570/148‑1 (MQ=255)
                                                                                                                        gATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                        <  1:1795803/148‑1 (MQ=255)
                                                                                                                         aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       <  2:2253263/148‑1 (MQ=255)
                                                                                                                         aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       >  2:2962437/1‑148 (MQ=255)
                                                                                                                          ttttGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTg                      <  1:2361621/148‑1 (MQ=255)
                                                                                                                            ttGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                    >  2:1838419/1‑148 (MQ=255)
                                                                                                                              gTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCg                  >  2:222192/1‑148 (MQ=255)
                                                                                                                              gTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCg                  >  2:2018807/1‑148 (MQ=255)
                                                                                                                                 ggCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTc               >  1:3023004/1‑148 (MQ=255)
                                                                                                                                  gCCTGATGAGCGTAGAGCATCAGGCACTTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTCCCTTCATCAACGTCTCGTAAGCGACGGTTTTGTCCGCTCg              >  2:1392300/1‑148 (MQ=255)
                                                                                                                                      gatgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaag          >  2:1192361/1‑148 (MQ=255)
                                                                                                                                      gatgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaag          >  2:2759215/1‑148 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         >  1:13297/1‑148 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         <  2:1103590/148‑1 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         <  1:3116135/148‑1 (MQ=255)
                                                                                                                                        tgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaagaa        <  2:2371317/148‑1 (MQ=255)
                                                                                                                                          aGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAagaaga      >  2:2586268/1‑148 (MQ=255)
                                                                                                                                           gCGTATAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAt     <  2:3030498/148‑1 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    <  1:142951/148‑1 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  1:529871/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144011‑3144306
                                                                                                                                                   |                                                                                                                                                    
GTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGC                                                                                                                                                    >  SRR19973067.607496/1‑150 (MQ=60)
     AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                               >  SRR19973067.1111092/1‑150 (MQ=60)
     AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                               >  SRR19973067.1111114/1‑150 (MQ=60)
      ATAGATTTTGTAGGCCTGATAAGCGTCGCGAATCAGTCATTGAGCACCTAATGCCGGATGCGTCTGTAACGCCTTATCCGGCCTACAAAAGCACGAAGATTCAACATGTTGCAATAGAGTTTGTAGGACTGAAGAGCGTCGAGCATCAGG                                                                                                                                              >  SRR19973067.2077296/1‑150 (MQ=60)
      ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGG                                                                                                                                              >  SRR19973067.2657189/1‑150 (MQ=60)
      ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGG                                                                                                                                              >  SRR19973067.270370/1‑150 (MQ=60)
        AGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCA                                                                                                                                            >  SRR19973067.3148777/1‑150 (MQ=60)
         GATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAA                                                                                                                                           >  SRR19973067.2386437/1‑150 (MQ=60)
         CATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAA                                                                                                                                           >  SRR19973067.2386740/1‑150 (MQ=60)
           TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATT                                                                                                                                         <  SRR19973067.2710548/150‑1 (MQ=60)
            TTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTC                                                                                                                                        <  SRR19973067.2368001/150‑1 (MQ=60)
              TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                      >  SRR19973067.439560/1‑150 (MQ=60)
              TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                      <  SRR19973067.921041/150‑1 (MQ=60)
                TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                    >  SRR19973067.1535739/1‑150 (MQ=60)
                 AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTT                                                                                                                                   <  SRR19973067.1963962/150‑1 (MQ=60)
                 AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTT                                                                                                                                   <  SRR19973067.3354960/150‑1 (MQ=60)
                  GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                  <  SRR19973067.2130037/150‑1 (MQ=60)
                  GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                  <  SRR19973067.2129923/150‑1 (MQ=60)
                  GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                  <  SRR19973067.688334/150‑1 (MQ=60)
                    CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCACTTCTACTTTAC                                                                                                                                >  SRR19973067.3499062/1‑150 (MQ=60)
                     CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                               <  SRR19973067.2938678/150‑1 (MQ=60)
                     CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                               <  SRR19973067.1114111/150‑1 (MQ=60)
                      TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                              >  SRR19973067.2749449/1‑150 (MQ=60)
                      TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                              <  SRR19973067.2665991/150‑1 (MQ=60)
                      TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                              <  SRR19973067.3296392/150‑1 (MQ=60)
                       GATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTC                                                                                                                             >  SRR19973067.194432/1‑150 (MQ=60)
                        ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                            <  SRR19973067.1002843/150‑1 (MQ=60)
                        ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                            >  SRR19973067.465238/1‑150 (MQ=60)
                          AAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCT                                                                                                                          <  SRR19973067.1130210/150‑1 (MQ=60)
                           AGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTT                                                                                                                         >  SRR19973067.868981/1‑150 (MQ=60)
                           AGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTT                                                                                                                         >  SRR19973067.812496/1‑150 (MQ=60)
                              GTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGG                                                                                                                      >  SRR19973067.1429272/1‑150 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 <  SRR19973067.440066/150‑1 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 >  SRR19973067.1680807/1‑150 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 <  SRR19973067.596023/150‑1 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 <  SRR19973067.596323/150‑1 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 <  SRR19973067.313719/150‑1 (MQ=60)
                                   GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                 <  SRR19973067.776569/150‑1 (MQ=60)
                                    CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTT                                                                                                                >  SRR19973067.440395/1‑150 (MQ=60)
                                     ATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTC                                                                                                               >  SRR19973067.2075827/1‑150 (MQ=60)
                                      TCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                              <  SRR19973067.2500571/150‑1 (MQ=60)
                                      TCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                              <  SRR19973067.2055734/150‑1 (MQ=60)
                                      TCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                              <  SRR19973067.2055712/150‑1 (MQ=60)
                                      TCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                              <  SRR19973067.2660386/150‑1 (MQ=60)
                                      TCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                              <  SRR19973067.3164821/150‑1 (MQ=60)
                                       CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                             >  SRR19973067.3099327/1‑150 (MQ=60)
                                       CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                             >  SRR19973067.3099326/1‑150 (MQ=60)
                                       CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                             >  SRR19973067.3099487/1‑150 (MQ=60)
                                        AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                            <  SRR19973067.597194/150‑1 (MQ=60)
                                          GCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGC                                                                                                          <  SRR19973067.1750512/150‑1 (MQ=60)
                                           CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                         >  SRR19973067.588180/1‑150 (MQ=60)
                                           CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                         <  SRR19973067.2104882/150‑1 (MQ=60)
                                            ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                        >  SRR19973067.2146535/1‑150 (MQ=60)
                                            ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                        >  SRR19973067.1661969/1‑150 (MQ=60)
                                               GAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCagatcgg                                                                                                     >  SRR19973067.730273/1‑143 (MQ=60)
                                                  CACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGA                                                                                                  <  SRR19973067.1250674/150‑1 (MQ=60)
                                                  CACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGA                                                                                                  >  SRR19973067.1172668/1‑150 (MQ=60)
                                                  CACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGA                                                                                                  >  SRR19973067.1172593/1‑150 (MQ=60)
                                                   ACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAC                                                                                                 <  SRR19973067.1941905/150‑1 (MQ=60)
                                                   ACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAC                                                                                                 <  SRR19973067.3237840/150‑1 (MQ=60)
                                                     CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               <  SRR19973067.1211066/150‑1 (MQ=60)
                                                     CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               <  SRR19973067.2250676/150‑1 (MQ=60)
                                                     CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                               >  SRR19973067.2401187/1‑150 (MQ=60)
                                                          GCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTT                                                                                          <  SRR19973067.945102/150‑1 (MQ=60)
                                                           CCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTC                                                                                         <  SRR19973067.2354383/150‑1 (MQ=60)
                                                             GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                       >  SRR19973067.1188336/1‑150 (MQ=60)
                                                             GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                       >  SRR19973067.2972883/1‑150 (MQ=60)
                                                               ATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGT                                                                                     <  SRR19973067.3520770/150‑1 (MQ=60)
                                                                TGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTA                                                                                    >  SRR19973067.1161332/1‑150 (MQ=60)
                                                                TGCGTCGTGAACGCCTAATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTA                                                                                    >  SRR19973067.917491/1‑150 (MQ=60)
                                                                       TGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCC                                                                             <  SRR19973067.2980772/150‑1 (MQ=60)
                                                                        GAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCT                                                                            <  SRR19973067.1967465/150‑1 (MQ=60)
                                                                        GAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCT                                                                            <  SRR19973067.2111565/150‑1 (MQ=60)
                                                                        GAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCT                                                                            <  SRR19973067.2111585/150‑1 (MQ=60)
                                                                         AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                           >  SRR19973067.2443393/1‑150 (MQ=60)
                                                                           CGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                         <  SRR19973067.1677958/150‑1 (MQ=60)
                                                                            GCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATG                                                                        >  SRR19973067.2086653/1‑150 (MQ=60)
                                                                                TATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGC                                                                    >  SRR19973067.495291/1‑150 (MQ=60)
                                                                                TATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGC                                                                    >  SRR19973067.495297/1‑150 (MQ=60)
                                                                                TATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGC                                                                    >  SRR19973067.495310/1‑150 (MQ=60)
                                                                                 ATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCG                                                                   <  SRR19973067.1694800/150‑1 (MQ=60)
                                                                                       CCTACAAACGCACGAAGATTCAACATGTTGCAAGAGATATTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCA                                                             <  SRR19973067.1668867/150‑1 (MQ=60)
                                                                                         TACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCGTCAGGTAACCCGCCTGATGCAGCGTATCCATT                                                           <  SRR19973067.496737/150‑1 (MQ=60)
                                                                                             AACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCT                                                       >  SRR19973067.1092328/1‑150 (MQ=60)
                                                                                             AACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCT                                                       <  SRR19973067.2880641/150‑1 (MQ=60)
                                                                                               CGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTC                                                     <  SRR19973067.1757611/150‑1 (MQ=60)
                                                                                               CGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTC                                                     <  SRR19973067.1758327/150‑1 (MQ=60)
                                                                                               CGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTC                                                     <  SRR19973067.2587679/150‑1 (MQ=60)
                                                                                                GCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCA                                                    >  SRR19973067.2541256/1‑150 (MQ=60)
                                                                                                   CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                                 >  SRR19973067.2724356/1‑150 (MQ=60)
                                                                                                      AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                              >  SRR19973067.93271/1‑150 (MQ=60)
                                                                                                      AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                              >  SRR19973067.783821/1‑150 (MQ=60)
                                                                                                      AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                              >  SRR19973067.94448/1‑150 (MQ=60)
                                                                                                      AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                              <  SRR19973067.1856172/150‑1 (MQ=60)
                                                                                                         TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                           >  SRR19973067.1152285/1‑150 (MQ=60)
                                                                                                         TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                           >  SRR19973067.1154976/1‑150 (MQ=60)
                                                                                                         TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                           <  SRR19973067.694545/150‑1 (MQ=60)
                                                                                                         TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                           >  SRR19973067.613036/1‑150 (MQ=60)
                                                                                                         TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                           >  SRR19973067.611883/1‑150 (MQ=60)
                                                                                                          TCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTC                                          <  SRR19973067.1472754/150‑1 (MQ=60)
                                                                                                          TCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTC                                          <  SRR19973067.161286/150‑1 (MQ=60)
                                                                                                           CAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCG                                         <  SRR19973067.662756/150‑1 (MQ=60)
                                                                                                               ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                     >  SRR19973067.3133188/1‑150 (MQ=60)
                                                                                                               ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                     >  SRR19973067.1361244/1‑150 (MQ=60)
                                                                                                               ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                     >  SRR19973067.1361245/1‑150 (MQ=60)
                                                                                                                TGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATC                                    >  SRR19973067.3447850/1‑150 (MQ=60)
                                                                                                                TGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTATGGCGGTTTCTTCGCCGACAAGACCTATCTTAAGGTAACCCCCCATATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATC                                    >  SRR19973067.3447786/1‑150 (MQ=60)
                                                                                                                 GTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCG                                   >  SRR19973067.2994102/1‑150 (MQ=60)
                                                                                                                  TTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGA                                  >  SRR19973067.3073807/1‑150 (MQ=60)
                                                                                                                      AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGT                              >  SRR19973067.1845753/1‑150 (MQ=60)
                                                                                                                      AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCGTCATCAACGTCTCGTACTCGACGGT                              >  SRR19973067.1844926/1‑150 (MQ=60)
                                                                                                                        TAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTT                            <  SRR19973067.2144961/150‑1 (MQ=60)
                                                                                                                         AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                           <  SRR19973067.689757/150‑1 (MQ=60)
                                                                                                                          GATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTG                          <  SRR19973067.1807896/150‑1 (MQ=60)
                                                                                                                          GATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTG                          <  SRR19973067.1807882/150‑1 (MQ=60)
                                                                                                                            TTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTC                        <  SRR19973067.2377010/150‑1 (MQ=60)
                                                                                                                                 TAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTC                   >  SRR19973067.3042252/1‑150 (MQ=60)
                                                                                                                                     CCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAA               >  SRR19973067.231947/1‑150 (MQ=60)
                                                                                                                                      CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG              >  SRR19973067.1193437/1‑150 (MQ=60)
                                                                                                                                       TGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGA             >  SRR19973067.13506/1‑150 (MQ=60)
                                                                                                                                         ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG           >  SRR19973067.236701/1‑150 (MQ=60)
                                                                                                                                         ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG           <  SRR19973067.3135946/150‑1 (MQ=60)
                                                                                                                                              CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      >  SRR19973067.533429/1‑150 (MQ=60)
                                                                                                                                              CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT      <  SRR19973067.144208/150‑1 (MQ=60)
                                                                                                                                                 AGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT   >  SRR19973067.3309645/1‑150 (MQ=60)
                                                                                                                                                  GAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  <  SRR19973067.2050190/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTG  >  CP009273/3144011‑3144306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑